Maine Department of Environmental Protection Biological Monitoring Program Wetland Aquatic Life Classification Attainment Report Station Information Station Number: W-083 Trip ID:2002-083 River Basin: Maine Coastal Waterbody: PETTENGILL STREAM HUC8 Name: St. George-Sheepscot Town: Appleton Latitude: 44 17 59.1 N Mitigation Monitoring Site: No Longitude: 69 16 57.04 W Sample Information Sample ID: DN-2002-083 Type of Sample: DIPNET Sampling Organization: BIOMONITORING UNIT Date Sampled: 7/1/2002 Replicates: 3 Taxonomist: LOTIC INC. Subsample Factor: X1 Classification Attainment Statutory Class: A Final Determination: A Date: 12/18/2012 Model Result with P≥0.6: A Reason for Determination: Model Date Last Calculated: 12/18/2018 Comments: Model Probabilities First Stage Model C or Better Model Class A: 0.95 Class C: 0.00 Class A, B, or C 1.00 Class B: 0.05 NA: 0.00 Non-Attainment 0.00 B or Better Model A Model Class A or B 1.00 Class A 0.95 Class C or Non-Attainment 0.00 Class B or C or Non-Attainment 0.05 Model Variables Reference Range (10th or 90th percentile value) Abundance of Taxa in the Order Ephemeroptera 2.000 ≥ 0.000 Relative Richness of Taxa in the Orders Ephemeroptera, Odonata and Tricoptera 0.209 ≥ 0.140 Shannon-Wiener Diversity Index 4.080 ≥ 2.497 Relative Abundance of Collector-Gatherer Taxa 0.390 ≥ 0.131 Abundance of Sensitive Taxa 58.333 ≥ 3.424 Richness of Sensitive Taxa 9.000 ≥ 3.000 Maine Tolerance Index Score For Wetland Macroinvertebrates 24.409 ≤ 29.776 Ratio of MTI Sensitive to Eurytopic Taxa Abundance 12.500 ≥ 0.334

Other Variables Five Most Dominant Taxa Total Mean Abundance 176 Rank Taxon Name Percent Generic Richness: 43 1 Psectrocladius 22.73 Hilsenhoff Biotic Index: 7.66 2 Hyalella azteca 13.07 3 Lymnaeidae 10.23 4 Ablabesmyia 9.85 5 Procladius 6.44

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Surface Water Chemistry Sample Date: 7/1/2002 10:00:00 AM Collection Method Parameter Value Units Qualifier Grab Sample 3.3 mg/l Grab Sample Chloride 3 mg/l Grab Sample Chlorophyll A 0.0122 mg/l Grab Sample Dissolved Organic Carbon 21.8 mg/l Grab Sample 0.93 mg/l Grab Sample Nitrate As Nitrogen mg/l U Grab Sample pH 6 Grab Sample 0.44 mg/l Grab Sample 2 mg/l Grab Sample Specific Conductance 28.3 us/cm Grab Sample Sulfate mg/l U Grab Sample Total 6 mg/l Grab Sample Total Hardness 12 mg/l Grab Sample Total Kjeldahl Nitrogen (organic And Nh3) As Nitrogen 1 mg/l Grab Sample Total Phosphorus 0.041 mg/l Grab Sample True Color 200 unit In-situ Dissolved Oxygen 4.6 mg/l In-situ pH 6.75 In-situ Specific Conductance 28.8 us/cm In-situ Temperature 22.2 deg c Landcover Summary - 2004 Data

Total Area (ac) 3739 High Int. Dev. % 0.0 Water % 1.1 Non-vegetated % 0.9 Med Int. Dev. % 0.1 Wetland % 29.9 Tilled Agriculture % 0.1 Low Int. Dev. % 1.4 Upland Woody % 64.5 Grassland % 2.9 Development % 1.5 Natural % 92.7 Human Altered % 6.2 Impervious % 1.5 Total Land (ac) 3697 High Int. Dev. % 0.0 Water % N/A Non-vegetated % N/A Med Int. Dev. % 0.1 Wetland % 30.2 Tilled Agriculture % 0.1 Low Int. Dev. % 1.4 Upland Woody % 65.3 Grassland % 2.9 Development % 1.5 Natural % 93.8 Human Altered % 6.2 Impervious % 1.5

Monday, December 9, 2019 Page 2 Maine Department of Environmental Protection Biological Monitoring Program Wetland Aquatic Life Classification Attainment Report Summary of Habitat Characteristics Human Disturbance Landscape-level Cowardin Classification Hydrogeomorphic Setting Total Score: 5 System: PALUSTRINE Landscape Position: LOTIC STREAM Hydrologic Modifications: 2 Subsystem: Lotic Gradient: DAMMED REACH Vegetative Modifications: 1 Class 1: UNCONSOLIDATED Flow Path: THROUGHFLOW Chemical Contaminants 0 BOTTOM Impervious Surface: 2 Subclass 1: MUD Land Form: FRINGE Non-point Sources: 0 Class 2: Land Form Type: LOTIC STREAM FRINGE POND Subclass 2: Waterbody Type: POND Class 3: Waterbody Subtype: Subclass 3: Comments: Dominant Plant Species: PICKERELWEED (PONTEDERIA CORDATA), SEDGES, WATERSHIELD (BRASENIA), NUPHAR, POTAMOGETON SP. Additional Plant Community Observations: Habitat Classification: Substrate Classification: EMERGENT NON-PERSISTENT VEGETATION BOULDER SUBSTRATE DETRITUS SUBSTRATE SILT/MUCK SUBSTRATE Average Depth: 20 cm Sample Comments: TWO CONDUCTIVITY METERS USED, QUESTIONABLE METER READING IS 29. SULFATE SAMPLE DILUTED DUE TO INTERFERENCES. TOTAL ORGANIC CARBON RUN AS DISSOLVED ORGANIC CARBON.

Monday, December 9, 2019 Page 3 Maine Department of Environmental Protection Biological Monitoring Program Wetland Aquatic Life Classification Attainment Report Additional Summary Variables Station Number: W-083 Waterbody: PETTENGILL STREAM Town: Appleton Log Number: DN-2002-083 Subsample Factor: X1 Replicates: 3 Calculated: 12/18/2018 Abundance Relative Abundance Richness Relative Richness EOT Taxa: 11.67 0.066 9 0.21 EPT Taxa: 4.33 0.025 5 0.12 Insects: 125.67 0.714 34 0.79 Non-Insects: 50.33 0.286 9 0.21 Leeches: 0.67 0.004 2 0.05 Oligochaetes: 6.67 0.038 2 0.05 Snails: 19.00 0.108 2 0.05 Bivalves: 0.67 0.004 1 0.02 Isopods: 0.33 0.002 1 0.02 Amphipods: 23.00 0.131 1 0.02 Mites: 0.00 0.000 0 0.00 Stoneflies: 0.00 0.000 0 0.00 Mayflies: 2.00 0.011 3 0.07 Odonates: 7.33 0.042 4 0.09 Caddisflies: 2.33 0.013 2 0.05 Diptera: 107.67 0.612 19 0.44 Hemiptera: 5.00 0.028 4 0.09 Beetles: 1.33 0.008 2 0.05 Chironomids: 96.33 0.547 15 0.35 Tanypodinae Tribe: 39.00 0.222 5 0.12 Chironomiinae Tribe: 17.33 0.098 9 0.21 Orthoclodiinae Tribe: 40.00 0.227 1 0.02 Collector-Filterers: 5.33 0.030 2 0.05 Collector-Gatherers: 68.67 0.390 5 0.12 Predators: 63.00 0.358 16 0.37 Piercers: 1.33 0.008 1 0.02 Shredders: 5.00 0.028 4 0.09 Scrapers: 1.00 0.006 1 0.02 Maine Tolerance: Sensitive: 58.33 0.397 9 0.29 Intermediate: 84.00 0.571 16 0.52 Eurytopic: 4.67 0.032 6 0.19 Ratio of MTI 12.50 12.500 1.50 1.50 Sensitive to Eurytopic

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Aquatic Life Taxonomic Inventory Report Station Number: W-083 Waterbody: PETTENGILL STREAM Town: Appleton Log Number: DN-2002-083 Subsample Factor: X1 Replicates: 3 Calculated: 12/18/2018 Count Hilsen- Func- Maine Maine (Mean of hoff tional Toler- Taxonomic Samples) Biotic Feeding ance Taxon Code Actual Adjusted Index Group Index Tribe Taxa Group Naididae 08020202 3.33 3.33 ------Worm Stylaria 08020202014 3.33 3.33 -- CG 18-S -- Worm Glossiphoniidae 08030101 0.33 0.33 ------Leech Helobdella 08030101005 0.00 0.33 -- -- 43-E -- Leech Helobdella modesta 08030101005004 0.33 0.00 ------Leech Caecidotea 09010101001 0.33 0.33 8 SH 51.9-E -- Isopod Hyalella 09010203006 0.00 23.00 8 CG 24.5-I -- Amphipod Hyalella azteca 09010203006011 23.00 0.00 ------Amphipod Leucorrhinia 09020306034 2.33 2.33 9 PR 24.7-I -- Dragonfly/damselfly Lestes 09020308045 3.33 3.33 9 PR 32.6-I -- Dragonfly/damselfly Coenagrionidae 09020309 0.33 0.33 ------Dragonfly/damselfly Enallagma 09020309051 1.33 1.33 9 PR 26.2-I -- Dragonfly/damselfly Baetidae 09020401 0.33 0.33 ------Mayfly Siphlonuridae 09020405 0.67 0.67 ------Mayfly Caenis 09020412040 1.00 1.00 7 CG 22.1-I -- Mayfly Corixidae 09020501 2.33 2.33 ------True Bug Belostoma 09020502009 1.67 1.67 -- PR 41.7-I -- True Bug Notonectidae 09020505 0.67 0.67 ------True Bug Hydrometra 09020508023 0.33 0.33 -- PR -- -- True Bug Polycentropus 09020603010 1.00 1.00 6 PR 15.4-S -- Caddisfly Oxyethira 09020607028 1.33 1.33 3 P 22-S -- Caddisfly Culicoides 09021010037 0.33 0.33 10 PR 42.1-I -- Fly: Biting Midge Bezzia/palpomyia 09021010043 9.00 9.00 6 PR 26.9-I -- Fly: Biting Midge Ablabesmyia 09021011001 17.33 17.33 8 PR 23.6-I T Fly: Midge Guttipelopia 09021011006 6.00 6.00 5 PR 19.4-S T Fly: Midge Labrundinia 09021011008 2.33 2.33 7 PR 18.1-S T Fly: Midge Larsia 09021011009 2.00 2.00 6 PR 15.6-S T Fly: Midge Procladius 09021011015 11.33 11.33 9 PR 25.1-I T Fly: Midge Psectrocladius 09021011056 40.00 40.00 8 CG 22-S -- Fly: Midge Paratanytarsus 09021011071 1.67 1.67 6 -- 43-E Y Fly: Midge Tanytarsus 09021011076 4.67 4.67 6 CF 25.7-I Y Fly: Midge Cladopelma 09021011081 1.33 1.33 9 CG 27.9-I C Fly: Midge Cryptochironomus 09021011082 0.67 0.67 8 PR 31.3-I C Fly: Midge Cryptotendipes 09021011083 1.33 1.33 6 -- 7.1-S C Fly: Midge Endochironomus 09021011087 0.67 0.67 10 SH 50.2-E C Fly: Midge

Monday, December 9, 2019 Page 5 Maine Department of Environmental Protection Biological Monitoring Program Wetland Aquatic Life Classification Attainment Report Aquatic Life Taxonomic Inventory Report Station Number: W-083 Waterbody: PETTENGILL STREAM Town: Appleton Log Number: DN-2002-083 Subsample Factor: X1 Replicates: 3 Calculated: 12/18/2018 Count Hilsen- Func- Maine Maine (Mean of hoff tional Toler- Taxonomic Samples) Biotic Feeding ance Taxon Code Actual Adjusted Index Group Index Tribe Taxa Group Glyptotendipes 09021011088 0.67 0.67 10 SH 43-E C Fly: Midge Parachironomus 09021011097 3.00 3.00 10 PR 28.6-I C Fly: Midge Polypedilum 09021011102 3.33 3.33 6 SH 24.2-I C Fly: Midge Tabanidae 09021014 1.67 1.67 ------Fly: Horse And Deer Sciomyzidae 09021020 0.33 0.33 ------Fly: Marsh Dineutus 09021104032 0.33 0.33 2 -- 23.2-I -- Beetle Tropisternus 09021105038 1.00 1.00 -- PR 61.8-E -- Beetle Amnicola 10010104013 1.00 1.00 -- SC 18.7-S -- Snail Lymnaeidae 10010201 18.00 18.00 ------Snail Sphaeriidae 10020201 0.67 0.67 -- CF -- -- Clam

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