SAN TA C RUZ BI OTEC HNOL OG Y, INC . OR11H6 (V-12): sc-109985

BACKGROUND APPLICATIONS Olfactory receptors are G -coupled receptors that localize to the cilia OR11H6 (V-12) is recommended for detection of OR11H6 of human origin of olfactory sensory neurons where they display affinity for and bind to a by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), variety of odor molecules. The encoding olfactory receptors comprise immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and the largest family in the . The binding of solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000); non to odor molecules triggers a signal transduction that propagates cross-reactive with other OR11 family members. nerve impulses throughout the body, ultimately leading to transmission of the OR11H6 (V-12) is also recommended for detection of OR11H6 in additional signal to the brain and the subsequent perception of smell. OR11H6 (olfac - species, including equine, canine and porcine. tory receptor 11H6) is a 330 amino acid protein. The encoding OR411H6 maps to human 14. Suitable for use as control antibody for OR11H6 siRNA (h): sc-92447, OR11H6 shRNA Plasmid (h): sc-92447-SH and OR11H6 shRNA (h) Lentiviral Particles: REFERENCES sc-92447-V. 1. Malnic, B., Hirono, J., Sato, T. and Buck, L.B. 1999. Combinatorial receptor codes for odors. Cell 96: 713-723. RECOMMENDED SECONDARY REAGENTS To ensure optimal results, the following support (secondary) reagents are 2. Glusman, G., Bahar, A., Sharon, D., Pilpel, Y., White, J. and Lancet, D. 2000. recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 The olfactory receptor gene superfamily: data mining, classification, and (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey nomenclature. Mamm. Genome 11: 1016-1023. anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ 3. Gaillard, I., Rouquier, S. and Giorgi, D. 2004. Olfactory receptors. Cell. Mol. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: Life Sci. 61: 456-469 . sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- 4. Buck, L.B. 2004. Olfactory receptors and odor coding in mammals. Nutr. Rev. 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) 62: S184-S188. with UltraCruz™ Mounting Medium: sc-24941. 5. Malnic, B., Godfrey, P.A. and Buck, L.B. 2004. The human olfactory receptor gene family. Proc. Natl. Acad. Sci. USA 101: 2584-2589. PROTOCOLS 6. Khafizov, K., Anselmi, C., Menini, A. and Carloni, P. 2007. Ligand specificity See our web site at www.scbt.com or our catalog for detailed protocols and of odorant receptors. J. Mol. Model. 13: 401-409. support products. 7. Rinaldi, A. 2007. The scent of life. The exquisite complexity of the sense of smell in animals and humans. EMBO Rep. 8: 629-633.

CHROMOSOMAL LOCATION Genetic locus: OR11H6 (human) mapping to 14q11.2.

SOURCE OR11H6 (V-12) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an extracellular domain of OR11H6 of human origin.

PRODUCT Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-109985 P, (100 µg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).

STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.

RESEARCH USE For research use only, not for use in diagnostic procedures.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com