Affinity Biosciences website:www.affbiotech.com order:[email protected] GIPC2 Antibody

Cat.#: DF6357 Concn.: 1mg/ml Mol.Wt.: 34kDa Size: 100ul,200ul Source: Rabbit Clonality: Polyclonal

Application: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000 *The optimal dilutions should be determined by the end user. Reactivity: Human,Mouse,Rat

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity: GIPC2 Antibody detects endogenous levels of total GIPC2.

Immunogen: A synthesized peptide derived from human GIPC2, corresponding to a region within C-terminal amino acids.

Uniprot: Q8TF65

Description: The eukaryotic PDZ domain is a multifunctional - protein interacting motif that is found in a variety of and is involved in both the clustering of signaling molecules and the organization of protein networks. GIPC2 (GIPC PDZ domain containing family, member 2), also known as SEMCAP2, is a 315 amino acid protein that localizes to the cytoplasm and contains one PDZ domain. Expressed at high levels in kidney and colon and at lower levels in adult liver, GIPC2 interacts with SEMA5A and is thought to function as a scaffold protein, possibly modulating cell adhesion and growth factor signaling and playing a role in tumorigenesis. The encoding GIPC2 maps to human 1, which spans 260 million base pairs, contains over 3,000 and comprises nearly 8% of the .

Storage Condition and Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM Buffer: NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Western blot analysis of extracts from Myeloma cells, using GIPC2 Antibody. The lane on the left was treated with blocking peptide.

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Western blot analysis of 293 cell lysates, using GIPC2 Antibody. The lane on the left was treated with the antigen- specific peptide.

DF6357 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

For Research Use Only. Not for use in diagnostic and therapeutic procedures. Not for resale without express authorization.

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