Product datasheet Anti-NCKAP1 Antibody Catalog Number: A09238-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-NCKAP1 Antibody Name NCKAP1 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human NCKAP1 recombinant (Position: S2-K201). Human NCKAP1 shares 98.5% and 98% amino acid (aa) sequence identity with mouse and rat NCKAP1, respectively. Purification Immunogen affinity purified. Observed MW 129KD Dilution Ratios Western blot: 1:500-2000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Nck-associated protein 1 is a protein that in humans is encoded by the NCKAP1 gene. By somatic cell hybrid analysis, the NCKAP1 gene was mapped to 2. NCKAP1 is a predicted type II transmembrane protein, with a C-terminal transmembrane domain. It was demonstrated that suppression of NCKAP1 expression using an antisense NCKAP1 oligonucleotide induced apoptosis of neuronal cells.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-NCKAP1 Antibody Catalog Number: A09238-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot analysis of NCKAP1 using anti- NCKAP1 antibody (A09238-1). Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: MCF-7 whole Cell lysates, Lane 4: 293T whole cell lysates. anti- NCKAP1 antigen affinity purified polyclonal antibody (Catalog # A09238-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for NCKAP1 at approximately 129KD. The expected band size for NCKAP1 is at 129KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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