Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article

ABSTRACT Fig 2: Transmission electron photomicrograph of monocyte of dog Present study comprised of 72 crossbred cows (group I= 60 endometritic and group II=12 healthy) at 30±2days postpartum. The showing heterochromatin (a), euchromatin (b), cytoplasmic process (c), polymorphonuclear neutrophils (PMN) cell coun  Vacuole and nuclear membrane. Uranyl acetate and lead citrate × 25500

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

ASSOCIATION OF SEMEN TRAITS IN CONSECUTIVE EJACULATES WITH FSH-β GENE POLYMORPHISM IN HOLSTEIN-FRIESIAN CROSSBRED BULLS FROM INDIA VIJAY KADAM, ABH

trus synchronizathod that synchronizes ovulations is Corresponding author: [email protected] Fig. 1. Histogram depicting frequency distribution of animal named briefly as “Ovsynch” (Pursley et al., 1995). The right score of respondents Clinical Article study was aimed to evaluate the efficacy of different methods of estrus sync

Fig. 1. Semilogarithmic plot of plasma concentration time profile of amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and i.m. administration in sheep (n=4)

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article Haryana Vet. (March, 2020) 59(SI), 134-135 Clinical Article Research Article

COMPARATIVE EFFICACY OF SYNCHRONIZATION PROTOCOLS FOR IMPROVING SUCCESSFUL SURGICAL EXCISION OF OVARIAN REMNANT IN A QUEEN CAT FERTILITY IN POSTPARTUM CROSSBRED DAIRY COWS 1 2 J. UMAMAGESWARI*, G. APARNA, N. ARUNMOZHI, S. RANGASAMY, P. SRIDEVI RATNAPARKHI A.R., DESHMUKH S.G.*, BIRADE H.S., KALE V.B., HARKAL S.B. and JADHAO A.D. and S. BALASUBRAMANIAN Fig. 1. Lateral view of ribs (13 numbers) of left side of adult female Bule bull (Boselaphus tragocamelus) Department of Animal Reproduction, Gynaecology and Obstetrics, Department of Clinics, Madras Veterinary College, Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India TamilNadu Veterinary and Animal Sciences University, Chennai-600 007, India Corresponding author: [email protected] Received: 20.03.2019; Accepted: 13.05.2019 Received: 03.08.2019; Accepted: 15.11.2019 182 ABSTRACT SUMMARY The objective of present research work was to study the efficacy of two different synchronization protocols for improving the fertility in A 2-year-old domestic short haired female cat was presented with intermittent estrus signs after being spayed three months back. A hormone A B postpartum crossbred cows. A totah protocol induces better estrus response and first service conception rate in postpartum cows. challenge assay was done to confirm the diagnosis of ovarian remnant syndrome and the case was managed by surgical excision of ovarian remnant. Keywords: Conception rate, Double PG, Ovsynch protocol Keywords: Cat, Ovarian Remnant Syndrome Fig. 1. (A) Resection of ovarian remnant in the queen Cat and (B) Ovarian remnant after Fig. 2. Ovarian remnant exhibiting formation process excision of the (10X magnification) CONTRIBUTORS MAY NOTE Ovariohysterectomy (OHE) is widely performed in confirmative of the presence of a functional corpora lutea queens as a means of population control and to eliminate on the (Wallace,1991). Based on the hormone assay, risk of incomplete resection of the ovary. Bilateral medical therapy or surgical removal of the ovarian unwanted behaviours associated with estrus. However, the case was confirmed as ovarian remnant syndrome. remnants are more common and in those animals with remnant. Medical therapy includes administration of l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians due to incomplete removal of ovarian tissue during unilateral remnants, right ovarian remnants are more synthetic progestogens or androgens or GnRH analogue engaged in Veterinary Profession. An exploratory celiotomy was performed using ovariohysterectomy, a long term complication that arises is injectable anaesthesia. The queen was premedicated with common, due to the anatomical position of the ovary (Olivara et al., 2012). Surgical therapy includes the ovarian remnant syndrome (ORS). The diagnosis of cranial to the kidney. However, in the present case it was examination of both the ovarian pedicles for retained or The estrus synchronizathod that synchronizes l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript Inj. Xylazine (@ 1mg/kg IM) and Inj. Ketamine (@5 ORS in queens involves overt signs of estrus including found to be left ovarian remnant. accessory . Thus, ovarian remnants in a cat could be ovulations is named briefly as “Ovsynch” (Pursley et al., writing/submission. mg/kg IM), and anaesthesia was maintained using a 1995). The study was aimed to evaluate the efficacy of vocalization, sexual receptivity, kneading and head combination of Inj. Ketamine (@ 5 mg/kg IV) and Inj. The diagnosis of ORS in queens involves a suspected based on history of previous OHE followed by behavioural evidence of estrus and hormonal assay may THE HARYANA VETERINARIAN different methods of estrus synchronisation protocols in l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of rubbing. On the other hand, some queens may show subtler Diazepam (@ 0.5mg/kg IV). By mid ventral approach, a combination of behavioural signs, vaginal cytology, and support the presence of ovarian remnants. crossbred postpartum cows. Veterinary Sciences, along with each article. signs of estrus compared to the bitch. Despite the signs of large celiotomy incision was made to improve the chance blood hormonal determinations. In an ovariohy- MATERIAL AND METHODS estrus being variable among queens with ORS, a of identification of ovarian tissue and the left ovary was sterctomized queen, vaginal smears will resemble those of REFERENCES Editors/Editorial Board Members are highly thankful to l After revision, please return the revised manuscript and rebuttal at the earliest. retrospective study from the Athens Veterinary Diagnostic a queen in anestrus whereas, presence of cornified cells A total of 20 healthy crossbred cows at 60-90 days ligated and excised. Post-operatively, the cat was treated Heffelfinger, D.J. (2006). Ovarian remnant in a 2-year-old queen. Can. Laboratory at the University of Georgia, 46 cases of with Tab. Cefpodoxime @ 20mg/kg PO for 5 days and she with absent or pyknotic nuclei indicate estrogenic Vet. J. 47: 165–167. all the distinguished referees who helped us in the l Please mention your article reference number in all correspondence for a quick ovarian remnants were submitted from 1988 to 1992; ORS recovered uneventfully. Histological examination of influence. Since, the changes in the smear are very subtle, Johnson, C.A. (2009). In: Nelson Reproductive system disorders RW th response. was found to be more common in cats (29 cases) than in excised tissue confirmed the presence of corpus luteum in it cannot be used to rule out ORS. Similarly, and Couto C.G. (Edts). Small Anim. Inter. Med. (4 Edn.) St. evaluation of articles. We request them to continue to Louis, p. 907. dogs (17 cases), with an average of 17 days to 9 years after the ovarian remnant (Fig. 2). ultrasonography is a poor diagnostic tool in small animal l We solicit your co-operation. OHE (Miller, 1995; Johnson, 2009). The present case with remnant ovary due to its small hypoechoic ovarian Miller, D.M. (1995).Ovarian remnant syndrome in dogs and cats: 46 extend their co-operation and be prompt in future to give Ovarian remnant syndrome is a disorder characterized cases (1988–1992). J. Vet. Diagn. Invest. 7: 572–574. l study discusses about the diagnosis and surgical mass. Hence, the most efficient diagnostic aid for ORS is a All correspondence should be addressed to 'The Editor', Haryana Veterinarian, by the presence of ovarian function in a previously Nelson, R.W. and Couto, C.G. (2003) Small Animal Internal Medicine. management of ORS in a queen cat. GnRH challenge test. The goal of this was to cause rd Department of Veterinary Public Health and Epidemiology, College of Veterinary ovariohysterctomized bitch or queen. According to Nelson (3 Edn.) St. Louis, Missouri: Mosby, pp. 851–869. their valuable comments on the articles for timely ovulation of a follicle by exogenous LH-like compounds, CONTRIBUTORS MAY NOTE Sciences, LUVAS, Hisar-125004. A 2-year-old domestic short hair queen cat was and Couto (2003), the most common cause of behavioural Olivara, K.S., Machado Silva, M.A, Brun,M.V., Jose Felix and Gilson THE HARYANA VETERINARIAN publication of the journal. presented to the Small Animal Obstetrics and Gynaecological and physical signs of estrus after ovario- in a thus confirming the presence of functional ovarian tissue H.T.(2012). Ovarian remnant syndrome in small animals. Editors unit of Madras Veterinary College with the history of cat is remnant ovarian tissue that has regained (Heffelfinger, 2006). Ciencias Agrarias, Londrina. 33: 363-380. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians intermittent estrus behaviour and increased vocalization folliculogenesis and production of estrogen. It occurs due Treatment of ORS includes leaving the ovarian Wallace, M.S. (1991). The ovarian remnant syndrome in the bitch and queen. Vet. Clin. North Am. 21: 501–507. Editors/Editorial Board Members are highly thankful to engaged in Veterinary Profession. for the past three months after being spayed. The owner to errors in surgical technique with the incomplete removal of remnant intact if the owner can tolerate estrus behaviour, also reported that spaying was done on the right flank one or both ovaries. The revascularization of ovarian l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript all the distinguished referees who helped us in the region. On clinical examination, all vital parameters were remnants or the presence of supernumerary or ectopic writing/submission. THE HARYANA VETERINARIAN within the normal range. The was shrunken with no ovaries in the abdomen can cause ORS (Miller, 1995). The evaluation of articles. We request them to continue to visible vaginal discharge. Due to high variability in length l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of ovarian tissue can remain hormonally functional and even of the estrous cycle in queens and due to subtle changes in Veterinary Sciences, along with each article. ovulate. Performing OHE via the left flank may have extend their co-operation and be prompt in future to give Editors/Editorial Board Members are highly thankful to the vaginal smears, vaginal exfoliative cytology was not limited access to the right ovary, thereby increasing the good technique to stage the cycle. Suspecting the case to be l After revision, please return the revised manuscript and rebuttal at the earliest. Table 1 their valuable comments on the articles for timely all the distinguished referees who helped us in the an ORS, challenge test was done wherein the cat was Serum Progesterone and Estrogen values in the queen cat l Please mention your article reference number in all correspondence for a quick injected with 2µg of GnRH analogue, Buserelin Acetate after GnRH Challenge test publication of the journal. evaluation of articles. We request them to continue to response. (Gynarich®) IM, single serum sample collected on day 7 and 14 post injection and analyzed for progesterone and Days post GnRH Progesterone Estrogen injection (ng/ml) (pg/ml) extend their co-operation and be prompt in future to give l We solicit your co-operation. CONTRIBUTORS MAY NOTE estrogen level (Table 1). Progesterone levels of more than 2ng/ml and estrogen levels of less than 20 pg/ml were 7 6 12.9 Corresponding author: [email protected] l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, *Corresponding author: [email protected] 14 25 9.2 their valuable comments on the articles for timely Department of Veterinary Public Health and Epidemiology, Collegel Research/Clinical of Veterinary articles are invited for next issue from the Scientists/Veterinarians publication of the journal. Sciences, LUVAS, Hisar-125004. engaged in Veterinary Profession. 134 135 l Please followEditors strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary CONTRIBUTORS MAY NOTE Sciences, LUVAS, Hisar-125004. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. THE HARYANA VETERINARIAN l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick Editors/Editorial Board Members are highly thankful to response. all the distinguished referees who helped us in the l We solicit your co-operation. evaluation of articles. We request them to continue to l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary extend their co-operation and be prompt in future to give Sciences, LUVAS, Hisar-125004. their valuable comments on the articles for timely Editors publication of the journal.

THE HARYANA VETERINARIAN THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the Editors/Editorial Board Members are highly thankful to evaluation of articles. We request them to continue to all the distinguished referees who helped us in the extend their co-operation and be prompt in future to give evaluation of articles. We request them to continue to their valuable comments on the articles for timely extend their co-operation and be prompt in future to give publication of the journal. their valuable comments on the articles for timely publication of the journal. THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the 39 CONTRIBUTORS MAY NOTE evaluation of articles. We request them to continue to extend their co-operation and be prompt in future to give l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. their valuable comments on the articles for timely l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript publication of the journal. writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article

ABSTRACT Fig 2: Transmission electron photomicrograph of monocyte of dog Present study comprised of 72 crossbred cows (group I= 60 endometritic and group II=12 healthy) at 30±2days postpartum. The showing heterochromatin (a), euchromatin (b), cytoplasmic process (c), polymorphonuclear neutrophils (PMN) cell coun  Vacuole and nuclear membrane. Uranyl acetate and lead citrate × 25500

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

ASSOCIATION OF SEMEN TRAITS IN CONSECUTIVE EJACULATES WITH FSH-β GENE POLYMORPHISM IN HOLSTEIN-FRIESIAN CROSSBRED BULLS FROM INDIA VIJAY KADAM, ABH

trus synchronizathod that synchronizes ovulations is Corresponding author: [email protected] Fig. 1. Histogram depicting frequency distribution of animal named briefly as “Ovsynch” (Pursley et al., 1995). The right score of respondents Clinical Article study was aimed to evaluate the efficacy of different methods of estrus sync

Fig. 1. Semilogarithmic plot of plasma concentration time profile of amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and i.m. administration in sheep (n=4)

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article Haryana Vet. (March, 2020) 59(SI), 134-135 Clinical Article Research Article

COMPARATIVE EFFICACY OF SYNCHRONIZATION PROTOCOLS FOR IMPROVING SUCCESSFUL SURGICAL EXCISION OF OVARIAN REMNANT IN A QUEEN CAT FERTILITY IN POSTPARTUM CROSSBRED DAIRY COWS 1 2 J. UMAMAGESWARI*, G. APARNA, N. ARUNMOZHI, S. RANGASAMY, P. SRIDEVI RATNAPARKHI A.R., DESHMUKH S.G.*, BIRADE H.S., KALE V.B., HARKAL S.B. and JADHAO A.D. and S. BALASUBRAMANIAN Fig. 1. Lateral view of ribs (13 numbers) of left side of adult female Bule bull (Boselaphus tragocamelus) Department of Animal Reproduction, Gynaecology and Obstetrics, Department of Clinics, Madras Veterinary College, Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India TamilNadu Veterinary and Animal Sciences University, Chennai-600 007, India Corresponding author: [email protected] Received: 20.03.2019; Accepted: 13.05.2019 Received: 03.08.2019; Accepted: 15.11.2019 182 ABSTRACT SUMMARY The objective of present research work was to study the efficacy of two different synchronization protocols for improving the fertility in A 2-year-old domestic short haired female cat was presented with intermittent estrus signs after being spayed three months back. A hormone A B postpartum crossbred cows. A totah protocol induces better estrus response and first service conception rate in postpartum cows. challenge assay was done to confirm the diagnosis of ovarian remnant syndrome and the case was managed by surgical excision of ovarian remnant. Keywords: Conception rate, Double PG, Ovsynch protocol Keywords: Cat, Ovarian Remnant Syndrome Fig. 1. (A) Resection of ovarian remnant in the queen Cat and (B) Ovarian remnant after Fig. 2. Ovarian remnant exhibiting formation process excision of the Corpus Luteum (10X magnification) CONTRIBUTORS MAY NOTE Ovariohysterectomy (OHE) is widely performed in confirmative of the presence of a functional corpora lutea queens as a means of population control and to eliminate on the ovary (Wallace,1991). Based on the hormone assay, risk of incomplete resection of the ovary. Bilateral medical therapy or surgical removal of the ovarian unwanted behaviours associated with estrus. However, the case was confirmed as ovarian remnant syndrome. remnants are more common and in those animals with remnant. Medical therapy includes administration of l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians due to incomplete removal of ovarian tissue during unilateral remnants, right ovarian remnants are more synthetic progestogens or androgens or GnRH analogue engaged in Veterinary Profession. An exploratory celiotomy was performed using ovariohysterectomy, a long term complication that arises is injectable anaesthesia. The queen was premedicated with common, due to the anatomical position of the ovary (Olivara et al., 2012). Surgical therapy includes the ovarian remnant syndrome (ORS). The diagnosis of cranial to the kidney. However, in the present case it was examination of both the ovarian pedicles for retained or The estrus synchronizathod that synchronizes l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript Inj. Xylazine (@ 1mg/kg IM) and Inj. Ketamine (@5 ORS in queens involves overt signs of estrus including found to be left ovarian remnant. accessory ovaries. Thus, ovarian remnants in a cat could be ovulations is named briefly as “Ovsynch” (Pursley et al., writing/submission. mg/kg IM), and anaesthesia was maintained using a 1995). The study was aimed to evaluate the efficacy of vocalization, sexual receptivity, kneading and head combination of Inj. Ketamine (@ 5 mg/kg IV) and Inj. The diagnosis of ORS in queens involves a suspected based on history of previous OHE followed by behavioural evidence of estrus and hormonal assay may THE HARYANA VETERINARIAN different methods of estrus synchronisation protocols in l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of rubbing. On the other hand, some queens may show subtler Diazepam (@ 0.5mg/kg IV). By mid ventral approach, a combination of behavioural signs, vaginal cytology, and support the presence of ovarian remnants. crossbred postpartum cows. Veterinary Sciences, along with each article. signs of estrus compared to the bitch. Despite the signs of large celiotomy incision was made to improve the chance blood hormonal determinations. In an ovariohy- MATERIAL AND METHODS estrus being variable among queens with ORS, a of identification of ovarian tissue and the left ovary was sterctomized queen, vaginal smears will resemble those of REFERENCES Editors/Editorial Board Members are highly thankful to l After revision, please return the revised manuscript and rebuttal at the earliest. retrospective study from the Athens Veterinary Diagnostic a queen in anestrus whereas, presence of cornified cells A total of 20 healthy crossbred cows at 60-90 days ligated and excised. Post-operatively, the cat was treated Heffelfinger, D.J. (2006). Ovarian remnant in a 2-year-old queen. Can. Laboratory at the University of Georgia, 46 cases of with Tab. Cefpodoxime @ 20mg/kg PO for 5 days and she with absent or pyknotic nuclei indicate estrogenic Vet. J. 47: 165–167. all the distinguished referees who helped us in the l Please mention your article reference number in all correspondence for a quick ovarian remnants were submitted from 1988 to 1992; ORS recovered uneventfully. Histological examination of influence. Since, the changes in the smear are very subtle, Johnson, C.A. (2009). In: Nelson Reproductive system disorders RW th response. was found to be more common in cats (29 cases) than in excised tissue confirmed the presence of corpus luteum in it cannot be used to rule out ORS. Similarly, and Couto C.G. (Edts). Small Anim. Inter. Med. (4 Edn.) St. evaluation of articles. We request them to continue to Louis, p. 907. dogs (17 cases), with an average of 17 days to 9 years after the ovarian remnant (Fig. 2). ultrasonography is a poor diagnostic tool in small animal l We solicit your co-operation. OHE (Miller, 1995; Johnson, 2009). The present case with remnant ovary due to its small hypoechoic ovarian Miller, D.M. (1995).Ovarian remnant syndrome in dogs and cats: 46 extend their co-operation and be prompt in future to give Ovarian remnant syndrome is a disorder characterized cases (1988–1992). J. Vet. Diagn. Invest. 7: 572–574. l study discusses about the diagnosis and surgical mass. Hence, the most efficient diagnostic aid for ORS is a All correspondence should be addressed to 'The Editor', Haryana Veterinarian, by the presence of ovarian function in a previously Nelson, R.W. and Couto, C.G. (2003) Small Animal Internal Medicine. management of ORS in a queen cat. GnRH challenge test. The goal of this was to cause rd Department of Veterinary Public Health and Epidemiology, College of Veterinary ovariohysterctomized bitch or queen. According to Nelson (3 Edn.) St. Louis, Missouri: Mosby, pp. 851–869. their valuable comments on the articles for timely ovulation of a follicle by exogenous LH-like compounds, CONTRIBUTORS MAY NOTE Sciences, LUVAS, Hisar-125004. A 2-year-old domestic short hair queen cat was and Couto (2003), the most common cause of behavioural Olivara, K.S., Machado Silva, M.A, Brun,M.V., Jose Felix and Gilson THE HARYANA VETERINARIAN publication of the journal. presented to the Small Animal Obstetrics and Gynaecological and physical signs of estrus after ovario-hysterectomy in a thus confirming the presence of functional ovarian tissue H.T.(2012). Ovarian remnant syndrome in small animals. Editors unit of Madras Veterinary College with the history of cat is remnant ovarian tissue that has regained (Heffelfinger, 2006). Ciencias Agrarias, Londrina. 33: 363-380. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians intermittent estrus behaviour and increased vocalization folliculogenesis and production of estrogen. It occurs due Treatment of ORS includes leaving the ovarian Wallace, M.S. (1991). The ovarian remnant syndrome in the bitch and queen. Vet. Clin. North Am. 21: 501–507. Editors/Editorial Board Members are highly thankful to engaged in Veterinary Profession. for the past three months after being spayed. The owner to errors in surgical technique with the incomplete removal of remnant intact if the owner can tolerate estrus behaviour, also reported that spaying was done on the right flank one or both ovaries. The revascularization of ovarian l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript all the distinguished referees who helped us in the region. On clinical examination, all vital parameters were remnants or the presence of supernumerary or ectopic writing/submission. THE HARYANA VETERINARIAN within the normal range. The vulva was shrunken with no ovaries in the abdomen can cause ORS (Miller, 1995). The evaluation of articles. We request them to continue to visible vaginal discharge. Due to high variability in length l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of ovarian tissue can remain hormonally functional and even of the estrous cycle in queens and due to subtle changes in Veterinary Sciences, along with each article. ovulate. Performing OHE via the left flank may have extend their co-operation and be prompt in future to give Editors/Editorial Board Members are highly thankful to the vaginal smears, vaginal exfoliative cytology was not limited access to the right ovary, thereby increasing the good technique to stage the cycle. Suspecting the case to be l After revision, please return the revised manuscript and rebuttal at the earliest. Table 1 their valuable comments on the articles for timely all the distinguished referees who helped us in the an ORS, challenge test was done wherein the cat was Serum Progesterone and Estrogen values in the queen cat l Please mention your article reference number in all correspondence for a quick injected with 2µg of GnRH analogue, Buserelin Acetate after GnRH Challenge test publication of the journal. evaluation of articles. We request them to continue to response. (Gynarich®) IM, single serum sample collected on day 7 and 14 post injection and analyzed for progesterone and Days post GnRH Progesterone Estrogen injection (ng/ml) (pg/ml) extend their co-operation and be prompt in future to give l We solicit your co-operation. CONTRIBUTORS MAY NOTE estrogen level (Table 1). Progesterone levels of more than 2ng/ml and estrogen levels of less than 20 pg/ml were 7 6 12.9 Corresponding author: [email protected] l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, *Corresponding author: [email protected] 14 25 9.2 their valuable comments on the articles for timely Department of Veterinary Public Health and Epidemiology, Collegel Research/Clinical of Veterinary articles are invited for next issue from the Scientists/Veterinarians publication of the journal. Sciences, LUVAS, Hisar-125004. engaged in Veterinary Profession. 134 135 l Please followEditors strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary CONTRIBUTORS MAY NOTE Sciences, LUVAS, Hisar-125004. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. THE HARYANA VETERINARIAN l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick Editors/Editorial Board Members are highly thankful to response. all the distinguished referees who helped us in the l We solicit your co-operation. evaluation of articles. We request them to continue to l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary extend their co-operation and be prompt in future to give Sciences, LUVAS, Hisar-125004. their valuable comments on the articles for timely Editors publication of the journal.

THE HARYANA VETERINARIAN THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the Editors/Editorial Board Members are highly thankful to evaluation of articles. We request them to continue to all the distinguished referees who helped us in the extend their co-operation and be prompt in future to give evaluation of articles. We request them to continue to their valuable comments on the articles for timely extend their co-operation and be prompt in future to give publication of the journal. their valuable comments on the articles for timely publication of the journal. THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the 39 CONTRIBUTORS MAY NOTE evaluation of articles. We request them to continue to extend their co-operation and be prompt in future to give l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. their valuable comments on the articles for timely l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript publication of the journal. writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors