Bhardwaj et al., Vegetos 2017, 30:1

Plant DOI: 10.4172/2229-4473.1000218 or Re F s y e t a e i r c c h Vegetos- An International o

S  

E 8 8 Journal of Research std. 19

Research Article a SciTechnol journal

investigation was undertaken to study the effect of number of nodes Effect of Node Number and Auxin per cutting and plant growth regulators on sprouting, rooting ability Concentration on Propagation of and establishment of rooted cuttings in ivy gourd. Material and Methods Ivy gourd ( cordifolia Field experiment was conducted at Research Farm, Indian Cogn) through Stem Cuttings Institute of Vegetable Research, Varanasi, () during 2010-11. The experiment was laid out in factorial randomized block design with Bhardwaj DR*, Rameshwar Singh, Hira Lal, Vishwa Nath and three replications. The treatments comprised of four nodes number Singh AK viz. 1, 2, 3 and 4 nodes per cutting and six concentrations of NAA and IBA viz. 25, 50, 75, 100, 125 and 150 ppm each. One year old pencil thickness stem cuttings were used. There were 144 nursery beds and Abstract 16 cuttings were planted in each nursery bed containing a mixture of Field experiment was conducted during 2010-2011 to study the soil: sand: compost (1:2:1) as per the treatments. Three cuttings were effect of number of nodes per cutting and concentrations of plant selected randomly for observations in each treatment and replications growth regulators (IBA and NAA) on regeneration of ivy gourd at and data were recorded on sprouting, shoot and root growth and Indian Institute of Vegetable Research, Varanasi. Cuttings with four regeneration of cuttings at 60 days after planting. nodes sprouted earliest (8.8 DAP) and enhanced the sprouting per cent, length and diameter of , number of branches per shoot, Results and Discussion number of leaves per cutting, length and diameter of root and regeneration per cent than one, two and three nodes per cutting. Ivy Effect of number of nodes per cutting gourd cuttings treated with 75 ppm IBA took minimum days to sprout and improved sprouting per cent, increased length and diameter of Number of nodes per cutting significantly influenced the vine, number of branches per shoot, number of leaves per cutting, sprouting, growth characters and regeneration of ivy gourd cuttings length and diameter of root and regeneration per cent than 75 ppm (Table 1). Cuttings with four nodes sprouted earliest (8.8 DAP) and NAA and 25, 50, 100, 125 and 150 ppm IBA and NAA each. had maximum percentage of sprouting (89.42%) than one, two and Keywords three nodes per cutting. In general, more the number of nodes per cutting, higher were the percentage of sprouting (Figure 1). Better Ivy gourd, Stem cutting, NAA, IBA, Regeneration performance of cuttings with higher nodes may be due to more food reserves which might have enhanced the sprouting percent. Similar Introduction results have been reported by Pandey and Ram in pointed gourd and Tripathi et al. in spine gourd. Similarly, cuttings with four nodes enhanced Ivy gourd (Coccinia cordifolia Cogn) is one of the important the length and diameter of vine, increased the number of branches per underexploited dioecious cucurbits, grown for its tender immature shoot, number of leaves per cutting, length and diameter of root and fruits for vegetable throughout the Asian countries. It is a semi- improved the regeneration per cent than one, two and three nodes per perennial crop and highly accepted by the people due to its availability cutting. These results are in close conformity with the findings of Pandey for nine months (February - October) in a year. In , ivy and Ram in pointed gourd and Sahu et al. in spine gourd. gourd is grown for its edible young shoots and fruits [1]. Being very rich in protein and , it has unique medicinal property viz. Effect of growth regulators (NAA and IBA) controlling diabetes, bronchitis, skin disorders, seeks fever [2] possess Concentration of growth regulators on ivy gourd cuttings cell-stabilizing, anti-anaphylactic and anti-histaminic potential [3]. significantly influenced the sprouting, growth characters and The survival of plant in nature is due to seed [4] but, propagation regeneration of ivy gourd cuttings (Table 1). In general, IBA proved through seed is avoided for commercial cultivation due to poor seed more effective at each concentration as compared to NAA (Figure 2). viability and establishment of , resulting more than 50 per cent Similar results were also reported by Ahmad in Kantola and Pandey non-fruiting i.e. male plants [5]. Vegetative propagation through stem and Ram in pointed gourd. However, cuttings treated with 75 ppm cuttings offers an excellent mean for commercial cultivation of this IBA took minimum days to sprout and improved the percentage of crop [6]. Success of vine cuttings varies with the number of nodes sprouted cuttings than 75 ppm NAA and 25, 50, 100, 125 and 150 ppm per cutting in spine gourd [7] and pointed gourd [8]. Further, use IBA and NAA each. However, stem cuttings treated with 75 ppm IBA of IBA enhances the sprouting, rooting ability and establishment enhanced the length and diameter of vine, increased the number of of rooted cuttings when treated with optimum concentrations in branches per shoot, number of leaves per cutting, length and diameter Kantola [9] spine gourd [10] and pointed gourd [8]. Thus, the present of root and improved the regeneration percent than 75 ppm NAA and 25, 50, 100, 125 and 150 ppm IBA and NAA each (Figure 3). The beneficial effects of auxin treatments may be due to the stimulation *Corresponding author: D. R. Bhardwaj, ICAR-Indian Institute of Vegetable Research, Varanasi, U.P., India, Tel: 9415301823; E-mail: dram_iivr@yahoo. of hydrolysis and mobilization of nutritional reserves to the region com of root formation [11]. These results are in close conformity with the Received: July 28, 2016 Accepted: February 16, 2017 Published: February findings of Pandey and Ram in pointed gourd and Tripathi et al. in 21, 2017 spine gourd.

All articles published in Vegetos: International Journal of Plant Research are the property of SciTechnol, and is protected by International Publisher of Science, copyright laws. Copyright © 2017, SciTechnol, All Rights Reserved. Technology and Medicine Citation: Bhardwaj DR, Singh R, Lal H, Nath V, Singh AK (2017) Effect of Node Number and Auxin Concentration on Propagation of Ivy gourd (Coccinia cordifolia Cogn) through Stem Cuttings. Vegetos 30:1.

doi:10.4172/2229-4473.1000218

Table 1: Effect of node number and plant growth regulators on regeneration of Ivy gourd cuttings. Diameter of Leaves/ Days to Length of vine Branches/ Root length Root diameter Regeneration Treatments Sprouting (%) vine cutting sprouting (No) (cm) shoot (No) (cm) (cm) (%) (cm) (No) Nodes /cutting One 64.56 10.9 13.1 0.40 4.0 34.5 15.2 0.08 55.06 Two 74.94 9.8 16.5 0.45 4.9 44.0 17.1 0.10 68.39 Three 82.89 9.1 19.3 0.46 5.0 52.4 17.9 0.13 75.97 Four 89.42 8.8 19.7 0.51 6.0 64.9 20.8 0.14 82.61 CD at 5% 1.99 0. 66 0.81 0.01 0.17 1.05 0.65 0.004 1.85 PGR concentration NAA 25 ppm 73.08 10.5 17.5 0.43 4.8 49.6 17.2 0.10 68.75 50 ppm 73.08 9.7 17.3 0.45 4.7 49.5 17.2 0.11 66.83 75 ppm 80.08 8.5 19.5 0.48 5.4 49.8 18.3 0.11 68.83 100 ppm 77.17 9.0 15.0 0.43 5.0 48.4 17.8 0.11 69.08 125 ppm 78.83 10.1 15.2 0.44 4.9 47.3 17.8 0.11 71.08 150 ppm 77.00 9.6 15.2 0.47 4.7 47.5 16.8 0.11 69.42 IBA 25 ppm 75.75 10.7 15.7 0.45 4.5 48.6 17.8 0.12 71.42 50 ppm 78.67 9.8 17.4 0.45 4.7 49.7 17.7 0.12 70.08 75 ppm 85.17 8.7 20.3 0.50 5.7 50.6 18.6 0.12 74.67 100 ppm 77.67 9.5 18.4 0.47 5.2 48.9 17.9 0.11 74.50 125 ppm 80.00 10.0 17.1 0.46 5.3 49.2 17.8 0.12 70.58 150 ppm 78.92 9.5 16.7 0.43 5.0 48.8 17.9 0.11 70.83 CD at 5% 3.45 1.20 1.40 0.02 0.29 1.81 NS 0.007 3.20

Figure 1: Number of node number per cutting.

Figure 2: Effect of NAA.

Volume 30 • Issue 1 • 1000218 • Page 2 of 3 • Citation: Bhardwaj DR, Singh R, Lal H, Nath V, Singh AK (2017) Effect of Node Number and Auxin Concentration on Propagation of Ivy gourd (Coccinia cordifolia Cogn) through Stem Cuttings. Vegetos 30:1.

doi:10.4172/2229-4473.1000218

Figure 3: Effect of IBA.

Refrences Sci. Pub Wageningen 150-151. 1. Linney G (1986) (L.) Voight: A new cucurbitaceous weed in 7. Sahu KC, Maharana T, Tripathy P, Parthi G (1995) Effect of type of cuttings Hawaii. Hawaii Botanical Society Newsletter 25: 3-5. and node number in spine gourd. Curr Res 24: 197-198.

2. Maurya RK (2013) Farmer-friendly ivy gourd. Indian Hort 58: 14-15. 8. Pandey AK, Ram D (2000) Effect of IBA, NAA and Node number in regeneration of pointed gourd ( dioica Roxb.) by stem cuttings. 3. Taur DJ, Patil RY (2011) Mast cell stabilizing, antianaphylactic and Progressive Horticulture 32: 172-175. antihistaminic activity of Coccinia grandis fruits in asthma. Chinese J. Natural Medicines 9: 359-362. 9. Ahmed T, Shukla PT, Chovatia VP, Makati JP (1992) Propagation of Kantola (Momordica dioica Roxb.) through cuttings by use of Indole Butyric Acid. 4. Lin LJ, Hsiao YY, Huang YS, Lu HL, Kuo CG (2006) Ivy gourd: development Gujrat Agrii Univ. J 17: 99-101. and promotion of tropical indigenous vegetables. Country Road 32: 2426. 10. Tripathi P, Maharana T, Dora DK (1996) Propagation of spine gourd 5. Singh K (1989) Pointed gourd ( Roxb.). Indian Hort 34: (Momordica dioca Roxb.) in relation to types of cutting, node number and IBA 34-37. concentration. Haryana J hort Sci 25: 92-94.

6. Boonkerd T, Songkhla BN, Thephuttee W (1993) Siemonsma JS and Piluek 11. Gregory FC, Samantarai B (1950) Factors concerned in the rooting responses K (eds) Prosea-plant resources of south-east Asia, No. 8, Vegetables. Pudoc of isolated leaves. J Exp Bot 1: 159-193.

Author Affiliations Top ICAR-Indian Institute of Vegetable Research, Varanasi, U.P., India

Submit your next manuscript and get advantages of SciTechnol submissions ™™ 80 Journals ™™ 21 Day rapid review process ™™ 3000 Editorial team ™™ 5 Million readers ™™ More than 5000 ™™ Quality and quick review processing through Editorial Manager System

Submit your next manuscript at ● www.scitechnol.com/submission

Volume 30 • Issue 1 • 1000218 • Page 3 of 3 •