PADI2 Antibody

CATALOG NUMBER: 30-805

Antibody used in WB on Human Hela at 1 ug/ml.

Specifications SPECIES REACTIVITY: Human TESTED APPLICATIONS: ELISA, WB APPLICATIONS: PADI2 antibody can be used for detection of PADI2 by ELISA at 1:12500. PADI2 antibody can be used for detection of PADI2 by western blot at 0.5 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. USER NOTE: Optimal dilutions for each application to be determined by the researcher. POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate PREDICTED MOLECULAR 75 kDa WEIGHT: IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PADI2. HOST SPECIES: Rabbit

Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. PHYSICAL STATE: Lyophilized BUFFER: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 uL of distilled water. Final antibody concentration is 1 mg/mL. CONCENTRATION: 1 mg/ml STORAGE CONDITIONS: For short periods of storage (days) store at 4˚C. For longer periods of storage, store PADI2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. CLONALITY: Polyclonal CONJUGATE: Unconjugated

Additional Info ALTERNATE NAMES: PADI2, KIAA0994, PAD-H19, PAD2, PDI2 ACCESSION NO.: NP_031391 PROTEIN GI NO.: 122939159 OFFICIAL SYMBOL: PADI2 ID: 11240

Background BACKGROUND: PADI2 encodes a member of the peptidyl deiminase family of , which catalyze the post- translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II is the most widely expressed family member. Known substrates for this enzyme include basic protein in the and in skeletal muscle and . PADI2 is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and , and it has also been implicated in glaucoma pathogenesis.This gene encodes a member of the peptidyl family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous . Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. REFERENCES: 1) Foulquier, C., (2007) Arthritis Rheum. 56 (11), 3541-3553.

FOR RESEARCH USE ONLY

December 12, 2016