Product Datasheet Anti- H1 [AE-4] antibody ab71594

1 Abreviews 6 References 1 Image

Overview

Product name Anti- [AE-4] antibody Description Mouse monoclonal [AE-4] to Histone H1 Specificity Histone H1 itself is a highly conserved and is practically identical in sequence across most species. Given this, species cross-reactivity may be expected. Tested applications IHC-Fr, IHC-P, ELISA, WB, ICC, ICC/IF, Electron Microscopy Species reactivity Reacts with: Human

Immunogen Nuclei from myeloid leukamia biopsy cells. Properties

Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. Storage buffer Preservative: 0.09% Sodium Azide Constituents: PBS Purity Protein A purified Clonality Monoclonal Clone number AE-4 Isotype IgG2a Research Areas Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Non Homol. End Joining Epigenetics and Nuclear Signaling H1 Unmodified Applications

Our Abpromise guarantee covers the use of ab71594 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes IHC-Fr IHC-Fr: Use at an assay dependent dilution. IHC-P IHC-P: Use at an assay dependent dilution. ELISA ELISA: Use at an assay dependent dilution. WB WB: 1/100. ICC ICC: Use at an assay dependent concentration. ICC/IF ICC/IF: Use at an assay dependent dilution. Electron Microscopy EM: Use at an assay dependent dilution. PubMed: 21063722 Target

Function May play a key role in the control of expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased

http://www.abcam.com/Histone-H1-AE-4-antibody-ab71594.html 1 Product Datasheet instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling. Tissue specificity Oocyte-specific. Sequence similarities Belongs to the histone H1/H5 family. Contains 1 H15 (linker histone H1/H5 globular) domain. Cellular localization Cytoplasm. Nucleus. .

Target information above from: UniProt accession Q8IZA3 The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. 38:D142-D148 (2010) .

Database links Gene: 132243 Human Entrez Gene: 3005 Human Entrez Gene: 3006 Human Entrez Gene: 3007 Human Entrez Gene: 3008 Human Entrez Gene: 3009 Human Entrez Gene: 3010 Human Entrez Gene: 3024 Human Entrez Gene: 341567 Human Entrez Gene: 8971 Human Omim: 142210 Human Omim: 142220 Human Omim: 142708 Human Omim: 142709 Human Omim: 142710 Human Omim: 142711 Human Omim: 602785 Human SwissProt: P07305 Human SwissProt: P10412 Human SwissProt: P16401 Human SwissProt: P16402 Human SwissProt: P16403 Human SwissProt: P22492 Human SwissProt: Q02539 Human SwissProt: Q147U8 Human SwissProt: Q2M2I2 Human SwissProt: Q3MJ34 Human SwissProt: Q3MJ42 Human SwissProt: Q4VB25 Human SwissProt: Q6FG88 Human SwissProt: Q6ISI1 Human SwissProt: Q7Z694 Human SwissProt: Q86WT7 Human SwissProt: Q8IZA3 Human SwissProt: Q8N6R3 Human SwissProt: Q92522 Human Unigene: 226117 Human Unigene: 97358 Human Alternative names dJ221C16.2 antibody dJ221C16.5 antibody DmeH1 antibody H1 0 histone antibody H1 antibody

http://www.abcam.com/Histone-H1-AE-4-antibody-ab71594.html 2 Product Datasheet H1 histone family member 0 antibody H1 histone family member 1 antibody H1 histone family member 3 antibody H1 histone family member 5 antibody H1 histone family member N testis specific antibody H1 histone family member O oocyte specific antibody H1 histone family member X antibody H1(0) antibody H1.1 antibody H1.2 antibody H1.3 antibody H1.4 antibody H1.5 antibody H10 antibody H1A antibody H1f0 antibody H1F0 protein antibody H1F1 antibody H1F2 antibody H1F3 antibody H1F4 antibody H1F5 antibody H1FNT antibody H1FOO antibody H1FOO protein antibody H1FOO_HUMAN antibody H1FT antibody H1FV antibody H1FX antibody H1oo antibody H1t antibody H1T2 antibody H1X antibody HANP1 antibody His1 antibody HisC antibody HIST1 antibody HIST1H1A antibody HIST1H1B antibody HIST1H1C antibody HIST1H1D antibody HIST1H1E antibody HIST1H1T antibody Histone 1 H1a antibody Histone 1 H1b antibody Histone 1 H1c antibody Histone 1 H1d antibody Histone 1 H1e antibody Histone 1 H1t antibody Histone antibody Histone H1' antibody Histone H1.0 antibody Histone H1.1 antibody Histone H1.2 antibody Histone H1.3 antibody Histone H1.4 antibody Histone H1.5 antibody http://www.abcam.com/Histone-H1-AE-4-antibody-ab71594.html 3 Product Datasheet Histone H1oo antibody Histone H1x antibody MGC116819 antibody MGC126630 antibody MGC126632 antibody MGC126642 antibody MGC138176 antibody MGC138345 antibody MGC15959 antibody MGC3992 antibody MGC50807 antibody MGC5241 antibody MGC8350 antibody Oocyte specific histone H1 antibody Oocyte specific linker histone H1 antibody Oocyte-specific histone H1 antibody Oocyte-specific linker histone H1 antibody osH1 antibody Testicular H1 histone antibody Anti-Histone H1 [AE-4] antibody images

ab71594 staining Histone H1 in HeLa cells by Immunocytochemistry/ Electron Microscopy. Cells were frozen under high pressure. Samples were incubated with ab71594 at 5µg/ml in 1% serum, 1% BSA, PBS. The secondary used was a donkey anti-mouse conjugated to 10nM gold, 1/30 dilution.

Immunocytochemistry - Histone H1 antibody [AE-4] (ab71594) Image courtesy of Margarita Sobol by Abreview.

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As standard Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery** Response to your inquiry within 24 hours

In addition We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards http://www.abcam.com/Histone-H1-AE-4-antibody-ab71594.html 4 Product Datasheet If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions

Guarantee only valid for products bought direct from Abcam or one of our authorized distributors **Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand, which operate a 120 day guarantee. Please contact your regional office for further details Antibodies tested only in recombinant samples are not covered for use on endogenous samples. Please check the datasheet for information on tested species "Predicted to react" information on datasheets is based on and provided for reference only "Predicted to react" species are not guaranteed, as they have not been tested Fast Track products are not covered under the Abpromise. Please see Fast Track terms and condition for additional information Abcam biochemicals are novel compounds and we have not tested their biological activity in house. Please use the literature to identify how to use these products effectively. If you require further assistance please contact the scientific support team

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