Product Information Sheet for NR-612

Bacillus mycoides, Strain NRS 273 Microbiological and Biomedical Laboratories. 5th ed. Washington, DC: U.S. Government Printing Office, 2007; see www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm. Catalog No. NR-612 (Derived from ATCC® 6462™) Disclaimers: You are authorized to use this product for laboratory research purposes only. It is not intended for use in humans. For research only. Not for human use.

Use of this product is subject to the terms and conditions of Contributor: ® the BEI Resources Material Transfer Agreement (MTA). The ATCC MTA is available on our Web site at www.beiresources.org.

Product Description: While BEI Resources uses reasonable efforts to include Classification: , accurate and up-to-date information on this product sheet, Species: Bacillus mycoides neither the ATCC nor the U.S. Government make any Type Strain: NRS 273 (CIP 103472, DSM 2048, NCTC warranties or representations as to its accuracy. Citations 12974) from scientific literature and patents are provided for informational purposes only. Neither ATCC nor the U.S. Material Provided: Government warrants that such information has been Each vial contains approximately 0.5 mL of bacterial culture confirmed to be accurate. in 0.5X Tryptic Soy Broth supplemented with 10% glycerol. This product is sent with the condition that you are Packaging/Storage: responsible for its safe storage, handling, use and disposal. NR-612 was packaged aseptically, in screw-capped plastic ATCC and the U.S. Government are not liable for any cryovials. The product is provided frozen and should be damages or injuries arising from receipt and/or use of this stored at -60°C or colder immediately upon arrival. For product. While reasonable effort is made to ensure long-term storage, the vapor phase of a liquid nitrogen authenticity and reliability of materials on deposit, the U.S. freezer is recommended. Freeze-thaw cycles should be Government, ATCC, their suppliers and contributors to BEI avoided. Resources are not liable for damages arising from the misidentification or misrepresentation of products. Growth Conditions: Media: Use Restrictions: Tryptic Soy Broth or equivalent This material is distributed for internal research, non- Tryptic Soy Agar or equivalent commercial purposes only. This material, its product or its Incubation: derivatives may not be distributed to third parties. Except as Temperature: 30°C performed under a U.S. Government contract, individuals Atmosphere: Aerobic contemplating commercial use of the material, its products or Propagation: its derivatives must contact the contributor to determine if a 1. Keep vial frozen until ready for use; then thaw. license is required. U.S. Government contractors may need 2. Transfer the entire thawed aliquot into a single tube of a license before first commercial sale. Tryptic Soy Broth. 3. Use several drops of the suspension to inoculate a References: Tryptic Soy Agar slant and/or plate. 1. Smith, N. R., R. E. Gordon and F. E. Clark. “Aerobic 4. Incubate the slant and/or plate at 30°C for 24 hours. Spore Forming Bacteria.” U.S. Dep. Agric. Monogr. 16 (1952): 1–148. Citation: 2. Demezas, D. H. and J. Bell. “Evaluation of Low Molecular Acknowledgment for publications should read “The following Weight RNA Profiles and Ribotyping to Differentiate reagent was obtained through the NIH Biodefense and Some Bacillus species.” Syst. Appl. Microbiol. 18 (1995): Emerging Infections Research Resources Repository, NIAID, 582–589. NIH: Bacillus mycoides, Strain NRS 273, NR-612.” 3. Bergeron, M. G., et al. “Highly Conserved Genes and Their Use to Generate Probes and Primers for Detection Biosafety Level: 1 of Microorganisms.” Infectio Diagnostic (I.D.I.) Inc., Appropriate safety procedures should always be used with assignee. International Patent WO/2001/023604. 05 Apr. this material. Laboratory safety is discussed in the following 2001. publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and ATCC® is a trademark of the American Type Culture Prevention, and National Institutes of Health. Biosafety in Collection.

Biodefense and Emerging Infections Research Resources Repository 800-359-7370 P.O. Box 4137 Fax: 703-365-2898 Manassas, VA 20108-4137 USA E-mail: [email protected] www.beiresources.org © 2005/2007 American Type Culture Collection (ATCC). All rights reserved. NR-612_18MAY2007 Page 1 of 1