Catalog # Aliquot Size
T893-40N-25 25 µg T893-40N-500 500 µg
Recombinant Human TWEAK (TNFSF12) Recombinant human protein expressed in E. coli cells
Catalog # T893-40N Lot # G3223-22
Product Description Purity
Recombinant Human TWEAK (TNFSF12) was expressed in E. coli Recombinant Human TWEAK cells. The protein accession number is O43508 (TNFSF12) resolved on a 15% SDS-
PAGE gel under reducing Gene Aliases conditions and stained with Coomassie Brilliant Blue G-250 TWEAK, Tumor necrosis factor ligand superfamily member 12, TNFSF12, DR3LG, Apo3-Ligand, Recombinant Human TNF-Related Approx. MW 17.2 kDa Weak Inducer of Apoptosis (TNFSF12)
Endotoxin Level
<1.0 EU/µg of recombinant protein as determined by the LAL method.
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
Reconstitution Protocol
Reconstitute at 0.1mg/ml/mL in sterile PBS containing at least 0.1% human or bovine serum albumin
Storage and Stability
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Scientific Background Recombinant Human TWEAK (TNFSF12) TWEAK is a TNF superfamily transmembrane protein which is Recombinant human protein expressed in E. coli cells expressed in many different cells and tissues, such as the heart, spleen and small intestine. Two different receptors have been Catalog Number T893-40N identified that bind TWEAK; the Death Receptor 3 (DR3) and the Specific Lot Number G3223-22 TWEAK Receptor (TWEAKR). While DR3 is expressed mainly in tissues Purity 95% that have high concentration of lymphocytes, the mitogen- Stability 6 mos. at –20oC from date of shipment inducible TWEAKR is has been shown to be expressed in fibroblasts, Storage & Shipping The lyophilized protein is stable for at hepetocellular carcinomas and endothelial cells. Recombinant least one year from date of receipt at - human TWEAK comprises the TNF homologous (secreted region) 70°C. Upon reconstitution, this cytokine of TWEAK. can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.