Catalog # Aliquot Size

T893-40N-25 25 µg T893-40N-500 500 µg

Recombinant Human TWEAK (TNFSF12) Recombinant human expressed in E. coli cells

Catalog # T893-40N Lot # G3223-22

Product Description Purity

Recombinant Human TWEAK (TNFSF12) was expressed in E. coli Recombinant Human TWEAK cells. The protein accession number is O43508 (TNFSF12) resolved on a 15% SDS-

PAGE gel under reducing Aliases conditions and stained with Coomassie Brilliant Blue G-250 TWEAK, ligand superfamily member 12, TNFSF12, DR3LG, Apo3-Ligand, Recombinant Human TNF-Related Approx. MW 17.2 kDa Weak Inducer of (TNFSF12)

Endotoxin Level

<1.0 EU/µg of recombinant protein as determined by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein

Reconstitution Protocol

Reconstitute at 0.1mg/ml/mL in sterile PBS containing at least 0.1% human or bovine serum albumin

Storage and Stability

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Scientific Background Recombinant Human TWEAK (TNFSF12) TWEAK is a TNF superfamily transmembrane protein which is Recombinant human protein expressed in E. coli cells expressed in many different cells and tissues, such as the heart, spleen and small intestine. Two different receptors have been Catalog Number T893-40N identified that bind TWEAK; the Death Receptor 3 (DR3) and the Specific Lot Number G3223-22 TWEAK Receptor (TWEAKR). While DR3 is expressed mainly in tissues Purity 95% that have high concentration of lymphocytes, the mitogen- Stability 6 mos. at –20oC from date of shipment inducible TWEAKR is has been shown to be expressed in fibroblasts, Storage & Shipping The lyophilized protein is stable for at hepetocellular carcinomas and endothelial cells. Recombinant least one year from date of receipt at - human TWEAK comprises the TNF homologous (secreted region) 70°C. Upon reconstitution, this cytokine of TWEAK. can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com

FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.