MATERIAL DATA SHEET

Recombinant Human His6 UBE2R2 Cat. # E2­715 ­conjugating Enzyme E2R 2 (UBE2R2), also known as CDC34B and UBC3B, is a 238 amino acid (aa) member of the Ubiquitin­conjugating (E2) enzyme family with a predicted molecular weight of 27 kDa. Human UBE2R2 shares 100% aa sequence identity with its mouse ortholog and 78% aa sequence identity with its paralog, UBCH3. UBE2Q2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The UBE1 Ubiquitin­ activating (E1) enzyme, but not the UBA6 E1 enzyme, is capable of charging UBE2R2 with Ubiquitin (2). UBE2R2 can be phosphorylated on Ser233 by , which promotes an interaction with the β­ TrCP (E3) and the subsequent ubiquitination and degradation of β­Catenin (1). This contains an N­terminal 6­His tag.

Product Information Quantity: 50 µg | 100 µg

MW: 28 kDa

Source: E. coli­derived Contains an N­terminal 6­His tag Accession # Q712K3

Stock: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP

Purity: >95%, by SDS­PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Rev. 5/7/2014 Page 1 of 2 www.bostonbiochem.com

Boston Biochem products are available via the R&D Systems distributor network. USA & CANADA Tel: (800) 343-7475 EUROPE Tel: +44 (0)1235 529449 CHINA Tel: +86 (21) 52380373 Use & Storage Use: Recombinant Human His6­UBE2R2 is a member of the Ubiquitin­conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin­activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate . Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6­UBE2R2 concentration of 0.1­ 1 μM.

Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. l 12 months from date of receipt, ­70 °C as supplied. l 3 months, ­70 °C under sterile conditions after opening.

Literature References: 1. Semplici, F. et al. (2002) Oncogene 21:3978. 2. Jin, J. et al. (2007) Nature 447:1135.

For research use only. Not for use in humans.

Rev. 5/7/2014 Page 2 of 2 www.bostonbiochem.com

Boston Biochem products are available via the R&D Systems distributor network. USA & CANADA Tel: (800) 343-7475 EUROPE Tel: +44 (0)1235 529449 CHINA Tel: +86 (21) 52380373