MATERIAL DATA SHEET
Recombinant Human His6 UBE2R2 Cat. # E2715 Ubiquitinconjugating Enzyme E2R 2 (UBE2R2), also known as CDC34B and UBC3B, is a 238 amino acid (aa) member of the Ubiquitinconjugating (E2) enzyme family with a predicted molecular weight of 27 kDa. Human UBE2R2 shares 100% aa sequence identity with its mouse ortholog and 78% aa sequence identity with its paralog, UBCH3. UBE2Q2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The UBE1 Ubiquitin activating (E1) enzyme, but not the UBA6 E1 enzyme, is capable of charging UBE2R2 with Ubiquitin (2). UBE2R2 can be phosphorylated on Ser233 by Casein Kinase 2, which promotes an interaction with the β TrCP Ubiquitin ligase (E3) and the subsequent ubiquitination and degradation of βCatenin (1). This protein contains an Nterminal 6His tag.
Product Information Quantity: 50 µg | 100 µg
MW: 28 kDa
Source: E. coliderived Contains an Nterminal 6His tag Accession # Q712K3
Stock: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Purity: >95%, by SDSPAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Rev. 5/7/2014 Page 1 of 2 www.bostonbiochem.com
Boston Biochem products are available via the R&D Systems distributor network. USA & CANADA Tel: (800) 343-7475 EUROPE Tel: +44 (0)1235 529449 CHINA Tel: +86 (21) 52380373 Use & Storage Use: Recombinant Human His6UBE2R2 is a member of the Ubiquitinconjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitinactivating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6UBE2R2 concentration of 0.1 1 μM.
Storage: Use a manual defrost freezer and avoid repeated freezethaw cycles. l 12 months from date of receipt, 70 °C as supplied. l 3 months, 70 °C under sterile conditions after opening.
Literature References: 1. Semplici, F. et al. (2002) Oncogene 21:3978. 2. Jin, J. et al. (2007) Nature 447:1135.
For research use only. Not for use in humans.
Rev. 5/7/2014 Page 2 of 2 www.bostonbiochem.com
Boston Biochem products are available via the R&D Systems distributor network. USA & CANADA Tel: (800) 343-7475 EUROPE Tel: +44 (0)1235 529449 CHINA Tel: +86 (21) 52380373