Fig. S1. Phylogenetic Relationships Among 350 Asparginase Proteins. an Unrooted Phylogenetic Tree Was Calculated Based on an MSA (Described in Material and Methods)

J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Fig. S1. Phylogenetic relationships among 350 asparginase proteins. An unrooted phylogenetic tree was calculated based on an MSA (described in Material and Methods). Main cellular divisions are indicated. The numbers at the nodes are percentages that indicate the levels of bootstrap support, based on 1,000 resample data sets. Only values ≥70% were considered. J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Distribution of type II L-asparaginases. Division Subdivision Total of asparaginases Percentage Actinobacteria 130 34.41 Proteobacteria Alphaproteobacteria 100 26.88 Betaproteobacteria 24 6.45 Deltaproteobacteria 3 0.81 Gammaproteobacteria 3 0.81 Cyanobacteria 38 10.48 Firmicutes Clostridia 13 3.49 Paenibacillus 10 2.69 Alicyclobacillus 20.54 Bacillales 2 0.54 Brevibacillus 20.54 Thermaerobacter 20.54 Carboxydothermus 10.27 Geobacillus 10.27 Halothermothrix 10.27 Heliobacterium 10.27 Lysinibacillus 10.27 Natranaerobius 10.27 Negativicutes 1 0.27 Deinococcus-thermus 10 2.69 Spirochaetes 2 0.54 Chlamydiae 1 0.27 Gemmatimonadetes 1 0.27 Green nonsulfur 1 0.27 Environment sample 4 1.08 Eukarya Ascomycetes 13 3.49 Cercozoa 1 0.27 Eudicots 2 0.54 Mosses 2 0.54

Division Subdivision Organism ID Eukarya Ascomycetes Aspergillus flavus NRRL3357 238506591 Eukarya Ascomycetes Aspergillus fumigatus A1163 159127448 Eukarya Ascomycetes Aspergillus fumigatus Af293 70999426 Eukarya Ascomycetes Aspergillus nidulans FGSC A4 67904416 Eukarya Ascomycetes Aspergillus niger CBS 513.88 145231238 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Eukarya Ascomycetes Aspergillus oryzae 83775992 Eukarya Ascomycetes Aspergillus oryzae RIB40 317158778 Eukarya Ascomycetes Gibberella zeae PH-1 46136617 Eukarya Ascomycetes Nectria haematococca mpVI 77-13-4 302895513 Eukarya Ascomycetes Neosartorya fischeri NRRL 181 119491510 Eukarya Cercozoa Paulinella chromatophora 194477260 Eukarya Ascomycetes Penicillium chrysogenum Wisconsin 54-1255 255941370 Eukarya Mosses Physcomitrella patens subsp. patens 168069789 Eukarya Mosses Physcomitrella patens subsp. patens 168070707 Eukarya Eudicots Ricinus communis 255604380 Eukarya Eudicots Ricinus communis 255604382 Eukarya Ascomycetes Talaromyces stipitatus ATCC 10500 242804081 Eukarya Ascomycetes Verticillium albo-atrum VaMs.102 302410363 Firmicutes Clostridia Acetivibrio cellulolyticus CD2 303240441 Firmicutes Alicyclobacillus Alicyclobacillus acidocaldarius LAA1 218289277 Firmicutes Alicyclobacillus Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 258512825 Firmicutes Bacillales Bacillus sp. 2 A 57 CT2 319652321 Firmicutes Bacillales Bacillus sp. B14905 126650948 Firmicutes Brevibacillus Brevibacillus brevis NBRC 100599 226311347 Firmicutes Brevibacillus Brevibacillus brevis NBRC 100599 226314167 Firmicutes Carboxydothermus Carboxydothermus hydrogenoformans Z-2901 78043850 Firmicutes Clostridia Clostridium cellulovorans 743B 302873329 Firmicutes Clostridia Clostridium cellulovorans 743B 302873330 Firmicutes Clostridia Clostridium kluyveri DSM 555 153953656 Firmicutes Clostridia Clostridium sp. D5 325263516 Firmicutes Clostridia Clostridium sp. L2-50 160895093 Firmicutes Clostridia Clostridium sticklandii DSM 519 310658069 Firmicutes Clostridia Coprococcus eutactus ATCC 27759 163815678 Firmicutes Clostridia Coprococcus sp. ART55 295093541 Firmicutes Clostridia Coprothermobacter proteolyticus DSM 5265 206896230 Firmicutes Clostridia Desulfotomaculum reducens MI-1 134299199 Firmicutes Clostridia Dethiobacter alkaliphilus AHT 1 225174478 Firmicutes Geobacillus Geobacillus sp. G11MC16 196250668 Firmicutes Halothermothrix Halothermothrix orenii H 168 220931024 Firmicutes Heliobacterium Heliobacterium modesticaldum Ice1 167629794 Firmicutes Lysinibacillus Lysinibacillus fusiformis ZC1 299535011 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Firmicutes Negativicutes Megamonas hypermegale ART12 291532430 Firmicutes Natranaerobius Natranaerobius thermophilus JW 188584952 Firmicutes Paenibacillus Paenibacillus curdlanolyticus YK9 304406104 Firmicutes Paenibacillus Paenibacillus larvae subsp. larvae B-3650 322384345 Firmicutes Paenibacillus Paenibacillus larvae subsp. larvae BRL-230010 167462814 Firmicutes Paenibacillus Paenibacillus sp. HGF5 329925748 Firmicutes Paenibacillus Paenibacillus sp. HGF5 329928575 Firmicutes Paenibacillus Paenibacillus sp. oral taxon 786 str. D14 253577384 Firmicutes Paenibacillus Paenibacillus sp. Y412MC10 261407733 Firmicutes Paenibacillus Paenibacillus sp. Y412MC10 261409319 Firmicutes Paenibacillus Paenibacillus vortex V453 315647849 Firmicutes Paenibacillus Paenibacillus vortex V453 315649474 Firmicutes Clostridia Peptoniphilus duerdenii ATCC BAA-1640 304438752 Firmicutes Thermaerobacter Thermaerobacter marianensis DSM 12885 317121113 Firmicutes Thermaerobacter Thermaerobacter subterraneus DSM 13965 313903541 Actinobacteria Actinomyces urogenitalis DSM 15434 227497609 Actinobacteria Actinosynnema mirum DSM 43827 256375702 Actinobacteria Aeromicrobium marinum DSM 15272 311744325 Actinobacteria Amycolatopsis mediterranei U32 300784065 Actinobacteria Amycolatopsis mediterranei U32 300790822 Actinobacteria Arthrobacter arilaitensis Re117 308178622 Actinobacteria Arthrobacter aurescens TC1 119961188 Actinobacteria Arthrobacter aurescens TC1 119961755 Actinobacteria Arthrobacter chlorophenolicus A6 220913930 Actinobacteria Arthrobacter chlorophenolicus A6 220914491 Actinobacteria Arthrobacter phenanthrenivorans Sphe3 325964661 Actinobacteria Arthrobacter phenanthrenivorans Sphe3 325965120 Actinobacteria Arthrobacter sp. FB24 116671950 Actinobacteria Arthrobacter sp. FB24 116672517 Actinobacteria Beutenbergia cavernae DSM 12333 229821888 Actinobacteria Brevibacterium linens BL2 260903924 Actinobacteria Brevibacterium mcbrellneri ATCC 49030 295395678 Actinobacteria Catenulispora acidiphila DSM 44928 256397338 Actinobacteria Cellulomonas flavigena DSM 20109 296130915 Actinobacteria Clavibacter michiganensis subsp. michiganensis NCPPB 382 148273348 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Actinobacteria Clavibacter michiganensis subsp. sepedonicus 170782542 Actinobacteria Corynebacterium glutamicum R 145296908 Actinobacteria Dermacoccus sp. Ellin185 309812570 Actinobacteria Frankia alni ACN14a 111220200 Actinobacteria Frankia sp. CcI3 86739054 Actinobacteria Frankia sp. EAN1pec 158318211 Actinobacteria Frankia sp. EUN1f 288918101 Actinobacteria Geodermatophilus obscurus DSM 43160 284992988 Actinobacteria Intrasporangium calvum DSM 43043 317126049 Actinobacteria Janibacter sp. HTCC2649 84498441 Actinobacteria Jonesia denitrificans DSM 20603 256832386 Actinobacteria Kineococcus radiotolerans SRS30216 152964081 Actinobacteria Kitasatospora setae KM-6054 311897750 Actinobacteria Kocuria rhizophila DC2201 184200073 Actinobacteria Kribbella flavida DSM 17836 284028970 Actinobacteria Kribbella flavida DSM 17836 284030253 Actinobacteria Kytococcus sed-eentarius DSM 20547 256824242 Actinobacteria Leifsonia xyli subsp. xyli str. CTCB07 50954874 Actinobacteria Marine actinobacterium PHSC20C1 88855346 Actinobacteria Microbacterium testaceum StLB037 323356852 Actinobacteria Micrococcus luteus NCTC 2665 239918336 Actinobacteria Micrococcus luteus SK58 289704665 Actinobacteria Microcystis aeruginosa NIES-843 166363295 Actinobacteria Microcystis aeruginosa PCC 7806 159026061 Actinobacteria Micromonospora aurantiaca ATCC 27029 302870148 Actinobacteria Micromonospora sp. ATCC 39149 238061303 Actinobacteria Micromonospora sp. L5 315503575 Actinobacteria Nakamurella multipartita DSM 44233 258651536 Actinobacteria Nakamurella multipartita DSM 44233 258655144 Actinobacteria Nocardia farcinica IFM 10152 54022548 Actinobacteria Nocardioidaceae bacterium Broad-1 326332009 Actinobacteria Nocardioidaceae bacterium Broad-1 326332373 Actinobacteria Nocardioides sp. JS614 119718423 Actinobacteria Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111 297559414 Actinobacteria Pseudonocardia sp. P1 324999719 Actinobacteria Renibacterium salmoninarum ATCC 33209 163840601 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Actinobacteria Rhodococcus equi 103S 312138306 Actinobacteria Rhodococcus equi ATCC 33707 325674776 Actinobacteria Rhodococcus erythropolis PR4 226308309 Actinobacteria Rhodococcus jostii RHA1 111021814 Actinobacteria Rhodococcus opacus B4 226364336 Actinobacteria Rothia dentocariosa ATCC 17931 311112177 Actinobacteria Rothia dentocariosa M567 300742261 Actinobacteria Rothia mucilaginosa ATCC 25296 255326689 Actinobacteria Rothia mucilaginosa DY-18 283457373 Actinobacteria Rubrobacter xylanophilus DSM 9941 108803916 Actinobacteria Saccharomonospora viridis DSM 43017 257057716 Actinobacteria Saccharopolyspora erythraea NRRL 2338 134101679 Actinobacteria Saccharopolyspora erythraea NRRL 2338 134103539 Actinobacteria Saccharopolyspora erythraea NRRL 2338 291003151 Actinobacteria Salinispora arenicola CNS-205 159036045 Actinobacteria Salinispora tropica CNB-440 145592881 Actinobacteria Stackebrandtia nassauensis DSM 44728 291303565 Actinobacteria Streptomyces albus J1074 239980443 Actinobacteria Streptomyces avermitilis MA-4680 29827857 Actinobacteria Streptomyces avermitilis MA-4680 29830568 Actinobacteria Streptomyces bingchenggensis BCW-1 297162188 Actinobacteria Streptomyces cf. griseus XylebKG-1 326778403 Actinobacteria Streptomyces clavuligerus ATCC 27064 254388555 Actinobacteria Streptomyces coelicolor A3(2) 21222582 Actinobacteria Streptomyces flavogriseus ATCC 33331 320009446 Actinobacteria Streptomyces ghanaensis ATCC 14672 239930424 Actinobacteria Streptomyces griseoaurantiacus M045 329941805 Actinobacteria Streptomyces griseoflavus Tu4000 302557056 Actinobacteria Streptomyces griseus subsp. griseus NBRC 13350 182437768 Actinobacteria Streptomyces hygroscopicus ATCC 53653 302540620 Actinobacteria Streptomyces lividans TK24 256786347 Actinobacteria Streptomyces pristinaespiralis ATCC 25486 297193549 Actinobacteria Streptomyces roseosporus NRRL 15998 239942374 Actinobacteria Streptomyces scabiei 87.22 290962651 Actinobacteria Streptomyces sp. AA4 302525305 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Actinobacteria Streptomyces sp. AA4 302531019 Actinobacteria Streptomyces sp. ACTE 282860451 Actinobacteria Streptomyces sp. C 302532611 Actinobacteria Streptomyces sp. SA3 actG 318059668 Actinobacteria Streptomyces sp. SPB74 295837498 Actinobacteria Streptomyces sp. SPB78 302520801 Actinobacteria Streptomyces sviceus ATCC 29083 297197644 Actinobacteria Streptomyces thermoluteus subsp. fuscus 311692183 Actinobacteria Streptomyces violaceusniger Tu 4113 307325565 Actinobacteria Streptomyces viridochromogenes DSM 40736 302549289 Actinobacteria Streptosporangium roseum DSM 43021 271962474 Actinobacteria Streptosporangium roseum DSM 43021 271967569 Actinobacteria Synechococcus elongatus PCC 6301 56751349 Actinobacteria Synechococcus sp. BL107 116070927 Actinobacteria Synechococcus sp. CB0101 318041821 Actinobacteria Synechococcus sp. CB0205 317970256 Actinobacteria Synechococcus sp. CC9311 113953369 Actinobacteria Synechococcus sp. CC9605 78213336 Actinobacteria Synechococcus sp. CC9902 78184415 Actinobacteria Synechococcus sp. JA-2-3B'a(2-13) 86608517 Actinobacteria Synechococcus sp. JA-3-3Ab 86605633 Actinobacteria Synechococcus sp. PCC 7002 170077710 Actinobacteria Synechococcus sp. PCC 7335 254423377 Actinobacteria Synechococcus sp. RCC307 148242083 Actinobacteria Synechococcus sp. RS9916 116072932 Actinobacteria Synechococcus sp. RS9917 87125674 Actinobacteria Synechococcus sp. WH 5701 87303377 Actinobacteria Synechococcus sp. WH 7803 148239761 Actinobacteria Synechococcus sp. WH 7805 88808798 Actinobacteria Synechococcus sp. WH 8102 33865368 Actinobacteria Synechococcus sp. WH 8109 260435365 Actinobacteria Synechocystis sp. PCC 6803 16331826 Actinobacteria Thermobispora bispora DSM 43833 296268471 Actinobacteria Thermomonospora curvata DSM 43183 269124922 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Actinobacteria Thermosynechococcus elongatus BP-1 22298606 Actinobacteria Verrucosispora maris AB-18-032 330470352 Actinobacteria Xylanimonas cellulosilytica DSM 15894 269957798 Proteobacteria Alpha Agrobacterium radiobacter K84 222085214 Proteobacteria Alpha Agrobacterium sp. H13-3 325292213 Proteobacteria Alpha Agrobacterium tumefaciens str. C58 159184486 Proteobacteria Alpha Agrobacterium vitis S4 222147878 Proteobacteria Alpha Ahrensia sp. R2A130 304391445 Proteobacteria Alpha Alpha proteobacterium BAL199 163793893 Proteobacteria Alpha Alpha proteobacterium HTCC2255 114769818 Proteobacteria Alpha Aurantimonas manganoxydans SI85-9A1 90418797 Proteobacteria Alpha Brucella abortus bv. 3 str. Tulya 254693323 Proteobacteria Alpha Brucella abortus S19 189023781 Proteobacteria Alpha Brucella abortus str. 2308 A 237815021 Proteobacteria Alpha Brucella ceti M490 256159271 Proteobacteria Alpha Brucella melitensis bv. 1 str. 16M 17987661 Proteobacteria Alpha Brucella ovis ATCC 25840 148559243 Proteobacteria Alpha Brucella pinnipedialis M163 254707728 Proteobacteria Alpha Brucella sp. 83 254718707 Proteobacteria Alpha Brucella sp. BO1 306845169 Proteobacteria Alpha Brucella sp. BO2 306842174 Proteobacteria Alpha Brucella sp. F5 261757776 Proteobacteria Alpha Brucella sp. NF 2653 306837755 Proteobacteria Alpha Brucella sp. NVSL 07-0026 294851924 Proteobacteria Alpha Brucella suis bv. 5 str. 513 254701351 Proteobacteria Alpha Candidatus Puniceispirillum marinum IMCC1322 294084144 Proteobacteria Alpha Fulvimarina pelagi HTCC2506 114704307 Proteobacteria Alpha Gluconacetobacter diazotrophicus PAl 5 162147045 Proteobacteria Alpha Gluconacetobacter diazotrophicus PAl 5 209544107 Proteobacteria Alpha Gluconobacter oxydans 621H 58039275 Proteobacteria Alpha Hoeflea phototrophica DFL-43 163760490 Proteobacteria Alpha Jannaschia sp. CCS1 89052757 Proteobacteria Alpha Labrenzia alexandrii DFL-11 254500917 Proteobacteria Alpha Loktanella vestfoldensis SKA53 84515770 Proteobacteria Alpha Magnetospirillum magnetotacticum MS-1 23005789 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Proteobacteria Alpha Mesorhizobium ciceri biovar biserrulae WSM1271 319784597 Proteobacteria Alpha Mesorhizobium loti MAFF303099 13476327 Proteobacteria Alpha Mesorhizobium opportunistum WSM2075 260462430 Proteobacteria Alpha Mesorhizobium sp. BNC1 110633208 Proteobacteria Alpha Oceanibulbus indolifex HEL-45 163745020 Proteobacteria Alpha Oceanicola batsensis HTCC2597 84500530 Proteobacteria Alpha Oceanicola granulosus HTCC2516 89070633 Proteobacteria Alpha Octadecabacter antarcticus 238 254449948 Proteobacteria Alpha Octadecabacter antarcticus 307 254439294 Proteobacteria Alpha Paracoccus denitrificans PD1222 119385230 Proteobacteria Alpha Pelagibaca bermudensis HTCC2601 114764494 Proteobacteria Alpha Phaeobacter gallaeciensis 2.10 163742504 Proteobacteria Alpha Phaeobacter gallaeciensis BS107 163738100 Proteobacteria Alpha Polymorphum gilvum SL003B-26A1 328544612 Proteobacteria Alpha Pseudovibrio sp. JE062 254471870 Proteobacteria Alpha Rhizobium etli Brasil 5 218508418 Proteobacteria Alpha Rhizobium etli CFN 42 6007496 Proteobacteria Alpha Rhizobium etli CFN 42 86356786 Proteobacteria Alpha Rhizobium etli CFN 42 86360624 Proteobacteria Alpha Rhizobium etli CIAT 652 190890847 Proteobacteria Alpha Rhizobium etli CIAT 652 190895595 Proteobacteria Alpha Rhizobium etli CIAT 894 218678528 Proteobacteria Alpha Rhizobium etli CIAT 894 218678558 Proteobacteria Alpha Rhizobium etli CNPAF512 327192924 Proteobacteria Alpha Rhizobium etli CNPAF512 327194327 Proteobacteria Alpha Rhizobium etli IE4771 218661204 Proteobacteria Alpha Rhizobium etli Kim 5 218461260 Proteobacteria Alpha Rhizobium leguminosarum bv. trifolii WSM1325 241203639 Proteobacteria Alpha Rhizobium leguminosarum bv. trifolii WSM2304 209546137 Proteobacteria Alpha Rhizobium leguminosarum bv. trifolii WSM2304 209548387 Proteobacteria Alpha Rhizobium leguminosarum bv. viciae 3841 116251047 Proteobacteria Alpha Rhodobacter capsulatus SB 1003 294675796 Proteobacteria Alpha Rhodobacter sp. SW2 260574033 Proteobacteria Alpha Rhodobacter sphaeroides 2.4.1 77464961 Proteobacteria Alpha Rhodobacter sphaeroides ATCC 17025 146276101 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Proteobacteria Alpha Rhodobacter sphaeroides ATCC 17029 126460830 Proteobacteria Alpha Rhodobacter sphaeroides KD131 221640882 Proteobacteria Alpha Rhodobacteraceae bacterium KLH11 254509869 Proteobacteria Alpha Rhodobacterales bacterium HTCC2083 254459860 Proteobacteria Alpha Rhodobacterales bacterium HTCC2150 126724737 Proteobacteria Alpha Rhodobacterales bacterium Y4I 254464983 Proteobacteria Alpha Rhodospirillum centenum SW 209966011 Proteobacteria Alpha Roseibium sp. TrichSKD4 307946095 Proteobacteria Alpha Roseobacter denitrificans OCh 114 110677602 Proteobacteria Alpha Roseobacter litoralis Och 149 163733194 Proteobacteria Alpha Roseobacter sp. AzwK-3b 149913211 Proteobacteria Alpha Roseobacter sp. CCS2 126733855 Proteobacteria Alpha Roseobacter sp. GAI101 254488049 Proteobacteria Alpha Roseobacter sp. MED193 86139437 Proteobacteria Alpha Roseobacter sp. SK209-2-6 126738621 Proteobacteria Alpha Roseomonas cervicalis ATCC 49957 296533358 Proteobacteria Alpha Roseovarius nubinhibens ISM 83952598 Proteobacteria Alpha Roseovarius sp. 217 85705891 Proteobacteria Alpha Roseovarius sp. TM1035 149203072 Proteobacteria Alpha Ruegeria pomeroyi DSS-3 56698173 Proteobacteria Alpha Ruegeria sp. R11 254476702 Proteobacteria Alpha Ruegeria sp. TM1040 99082856 Proteobacteria Alpha Sagittula stellata E-37 126729968 Proteobacteria Alpha Silicibacter lacuscaerulensis ITI-1157 260432253 Proteobacteria Alpha Silicibacter sp. TrichCH4B 259417440 Proteobacteria Alpha Sinorhizobium fredii NGR234 227821283 Proteobacteria Alpha Sinorhizobium medicae WSM419 150395794 Proteobacteria Alpha Sinorhizobium meliloti 1021 15964708 Proteobacteria Alpha Stappia aggregata IAM 12614 118593243 Proteobacteria Alpha Sulfitobacter sp. EE-36 83941900 Proteobacteria Alpha Sulfitobacter sp. NAS-14.1 83855376 Proteobacteria Alpha Thalassiobium sp. R2A62 255262104 Proteobacteria Alpha Uncultured alpha proteobacterium HF0010 30A23 297180865 Proteobacteria Beta Aromatoleum aromaticum EbN1 56478061 Proteobacteria Beta Burkholderia ambifaria AMMD 115358205 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Proteobacteria Beta Burkholderia ambifaria IOP40-10 170697773 Proteobacteria Beta Burkholderia ambifaria MC40-6 172062992 Proteobacteria Beta Burkholderia ambifaria MEX-5 171319095 Proteobacteria Beta Burkholderia cenocepacia AU 1054 107026652 Proteobacteria Beta Burkholderia cenocepacia J2315 206563041 Proteobacteria Beta Burkholderia cenocepacia MC0-3 170735843 Proteobacteria Beta Burkholderia dolosa AUO158 254253804 Proteobacteria Beta Burkholderia gladioli BSR3 330817060 Proteobacteria Beta Burkholderia glumae BGR1 238027266 Proteobacteria Beta Burkholderia graminis C4D1M 170694629 Proteobacteria Beta Burkholderia phymatum STM815 186473037 Proteobacteria Beta Burkholderia phytofirmans PsJN 187923420 Proteobacteria Beta Burkholderia sp. 383 78062835 Proteobacteria Beta Burkholderia sp. CCGE1001 323524970 Proteobacteria Beta Burkholderia sp. CCGE1002 295676123 Proteobacteria Beta Burkholderia sp. CCGE1003 307728727 Proteobacteria Beta Burkholderia sp. H160 209520244 Proteobacteria Beta Burkholderia sp. TJI49 325529471 Proteobacteria Beta Burkholderia ubonensis Bu 167589845 Proteobacteria Beta Burkholderia vietnamiensis G4 134293321 Proteobacteria Beta Burkholderia xenovorans LB400 91782800 Proteobacteria Beta Curvibacter putative symbiont of Hydra magnipapillata 260222630 Proteobacteria Gamma Marinomonas sp. MED121 87120917 Proteobacteria Gamma Marinomonas sp. MWYL1 152997724 Proteobacteria Gamma Reinekea sp. MED297 88797291 Chlamydiae Waddlia chondrophila WSU 86-1044 297620972 Cyanobacteria A. marina MBIC11017 158336571 Cyanobacteria Anabaena variabilis ATCC 29413 75906956 Cyanobacteria Arthrospira maxima CS-328 209526418 Cyanobacteria Arthrospira platensis NIES-39 291568392 Cyanobacteria Arthrospira platensis str. Paraca 284054162 Cyanobacteria Crocosphaera watsonii WH 8501 67921826 Cyanobacteria Cyanobacterium UCYN-A 284929646 Cyanobacteria Cyanobium sp. PCC 7001 254431133 Cyanobacteria Cyanothece sp. ATCC 51142 172035973 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Cyanobacteria Cyanothece sp. CCY0110 126657781 Cyanobacteria Cyanothece sp. PCC 7424 218438758 Cyanobacteria Cyanothece sp. PCC 7425 220909914 Cyanobacteria Cyanothece sp. PCC 7822 307150002 Cyanobacteria Cyanothece sp. PCC 8801 218247886 Cyanobacteria Cyanothece sp. PCC 8802 257060793 Cyanobacteria Cylindrospermopsis raciborskii CS-505 282898909 Cyanobacteria Gloeobacter violaceus PCC 7421 37522508 Cyanobacteria Lyngbya sp. PCC 8106 119493578 Cyanobacteria Microcoleus chthonoplastes PCC 7420 254412124 Cyanobacteria Nodularia spumigena CCY9414 119513470 Cyanobacteria Nostoc azollae' 0708 298491358 Cyanobacteria Nostoc punctiforme PCC 73102 186684459 Cyanobacteria Nostoc sp. PCC 7120 17231663 Cyanobacteria Oscillatoria sp. PCC 6506 300869174 Cyanobacteria Prochlorococcus marinus str. AS9601 123968582 Cyanobacteria Prochlorococcus marinus str. MIT 9202 254525906 Cyanobacteria Prochlorococcus marinus str. MIT 9211 159903487 Cyanobacteria Prochlorococcus marinus str. MIT 9215 157413417 Cyanobacteria Prochlorococcus marinus str. MIT 9301 126696387 Cyanobacteria Prochlorococcus marinus str. MIT 9303 124023086 Cyanobacteria Prochlorococcus marinus str. MIT 9312 78779363 Cyanobacteria Prochlorococcus marinus str. MIT 9313 33863097 Cyanobacteria Prochlorococcus marinus str. MIT 9515 123966129 Cyanobacteria Prochlorococcus marinus str. NATL1A 124025595 Cyanobacteria Prochlorococcus marinus str. NATL2A 72382062 Cyanobacteria Prochlorococcus marinus subsp. marinus str. CCMP1375 33240343 Cyanobacteria Prochlorococcus marinus subsp. pastoris str. CCMP1986 33861378 Cyanobacteria Raphidiopsis brookii D9 282898076 Cyanobacteria Trichodesmium erythraeum IMS101 113478024 Deinococcus-thermus Deinococcus deserti VCD115 226355283 Deinococcus-thermus Deinococcus deserti VCD115 315271257 Deinococcus-thermus Deinococcus proteolyticus MRP 325283819 Deinococcus-thermus Marinithermus hydrothermalis DSM 14884 328949938 Deinococcus-thermus Meiothermus ruber DSM 1279 291296446 J. Microbiol. Biotechnol. (2010), 20(11), 1301–1305 http://dx.doi.org/10.4014/jmb.1107.07047 First published online January 5, 2012 pISSN 1017-7825 eISSN 1738-8872

Table S1. Continued. Division Subdivision Organism ID Deinococcus-thermus Meiothermus silvanus DSM 9946 297567394 Deinococcus-thermus Thermus aquaticus Y51MC23 218296219 Deinococcus-thermus Thermus scotoductus SA-01 320449150 Deinococcus-thermus Thermus thermophilus HB27 46200240 Deinococcus-thermus Thermus thermophilus HB8 55980036 Deltaproteobacteria Bdellovibrio bacteriovorus HD100 42523915 Deltaproteobacteria Desulfarculus baarsii DSM 2075 302344566 Deltaproteobacteria Desulfobacca acetoxidans DSM 11109 328953638 Environment sample Uncultured bacterium 310772408 Environment sample Uncultured marine bacterium EB0 39F01 119713563 Environment sample Uncultured marine bacterium EB000 55B11 161170316 Environment sample Uncultured marine bacterium EB000 55B11 297183843 Gemmatimonadetes Gemmatimonas aurantiaca T-27 226227310 Green nonsulfur Anaerolinea thermophila UNI-1 320159722 Spirochaetes Treponema denticola ATCC 35405 42525716 Spirochaetes Treponema denticola F0402 325475170