Datasheet: MCA2118

Description: MOUSE ANTI HUMAN Specificity: GRANZYME B Format: Purified Product Type: Monoclonal Antibody Clone: GB7 Isotype: IgG2a Quantity: 0.1 mg

Product Details

Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Flow Cytometry Immunohistology - Frozen Immunohistology - Paraffin (1) 1/50 - 1/200 ELISA Immunoprecipitation Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Target Species Human

Product Form Purified IgG - liquid

Preparation Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution TRIS buffered saline

Preservative 0.01% Thiomersal Stabilisers

Approx. Protein IgG concentration 1.0 mg/ml Concentrations

Immunogen Recombinant Granzyme B.

External Database Links UniProt: P10144 Related reagents

Page 1 of 3 Entrez : 3002 GZMB Related reagents

Synonyms CGL1, CSPB, CTLA1, GRB

Fusion Partners Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Specificity Mouse anti Human Granzyme B antibody, clone GB7 recognizes the Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs)

Granzyme B plays a key role in the induction of by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.

Mouse anti Human Granzyme B antibody, clone GB7 has been reported to be suitable for Western Blotting.

References 1. Kummer, J.A. et al. (1993) Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins. J Immunol Methods. 163 (1): 77-83. 2. Nield, L.E. et al. (2002) Endocardial fibroelastosis associated with maternal anti-Ro and anti-La antibodies in the absence of atrioventricular block. J Am Coll Cardiol. 40: 796-802. 3. Himmelein, S. et al. (2015) Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia. J Virol. 89 (10): 5747-50. 4. Cohen, M. et al. (2017) Epstein-Barr virus lytic cycle involvement in diffuse large B cell lymphoma. Hematol Oncol. Jul 14 [Epub ahead of print]. 5. Sedelies, K.A. et al. (2004) Discordant regulation of granzyme H and granzyme B expression in human lymphocytes. J Biol Chem. 279 (25): 26581-7.

Storage Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life 18 months from date of despatch.

Health And Safety Material Safety Datasheet documentation #10098 available at: Information 10098: https://www.bio-rad-antibodies.com/uploads/MSDS/10098.pdf

Regulatory For research purposes only

Related Products Recommended Secondary Antibodies

Goat Anti Mouse IgG (STAR76...) RPE Goat Anti Mouse IgG IgA IgM (STAR87...) Alk. Phos., HRP Rabbit Anti Mouse IgG (STAR9...) FITC

Page 2 of 3 Goat Anti Mouse IgG (STAR77...) HRP Rabbit Anti Mouse IgG (STAR12...) RPE Goat Anti Mouse IgG (Fc) (STAR120...) FITC, HRP Rabbit Anti Mouse IgG (STAR8...) DyLight®800 Goat Anti Mouse IgG (STAR70...) FITC Human Anti Mouse IgG2a (HCA037...) FITC, HRP Rabbit Anti Mouse IgG (STAR13...) HRP Goat Anti Mouse IgG (H/L) (STAR117...) Alk. Phos., DyLight®488, DyLight®549, DyLight®649, DyLight®680, DyLight®800, FITC, HRP Recommended Negative Controls

MOUSE IgG2a NEGATIVE CONTROL (MCA929)

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'M317939:180717'

Printed on 01 Aug 2018

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