Datasheet: MCA2118
Description: MOUSE ANTI HUMAN GRANZYME B Specificity: GRANZYME B Format: Purified Product Type: Monoclonal Antibody Clone: GB7 Isotype: IgG2a Quantity: 0.1 mg
Product Details
Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Flow Cytometry Immunohistology - Frozen Immunohistology - Paraffin (1) 1/50 - 1/200 ELISA Immunoprecipitation Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Target Species Human
Product Form Purified IgG - liquid
Preparation Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution TRIS buffered saline
Preservative 0.01% Thiomersal Stabilisers
Approx. Protein IgG concentration 1.0 mg/ml Concentrations
Immunogen Recombinant Granzyme B.
External Database Links UniProt: P10144 Related reagents
Page 1 of 3 Entrez Gene: 3002 GZMB Related reagents
Synonyms CGL1, CSPB, CTLA1, GRB
Fusion Partners Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity Mouse anti Human Granzyme B antibody, clone GB7 recognizes the serine protease Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs)
Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.
Mouse anti Human Granzyme B antibody, clone GB7 has been reported to be suitable for Western Blotting.
References 1. Kummer, J.A. et al. (1993) Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins. J Immunol Methods. 163 (1): 77-83. 2. Nield, L.E. et al. (2002) Endocardial fibroelastosis associated with maternal anti-Ro and anti-La antibodies in the absence of atrioventricular block. J Am Coll Cardiol. 40: 796-802. 3. Himmelein, S. et al. (2015) Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia. J Virol. 89 (10): 5747-50. 4. Cohen, M. et al. (2017) Epstein-Barr virus lytic cycle involvement in diffuse large B cell lymphoma. Hematol Oncol. Jul 14 [Epub ahead of print]. 5. Sedelies, K.A. et al. (2004) Discordant regulation of granzyme H and granzyme B expression in human lymphocytes. J Biol Chem. 279 (25): 26581-7.
Storage Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life 18 months from date of despatch.
Health And Safety Material Safety Datasheet documentation #10098 available at: Information 10098: https://www.bio-rad-antibodies.com/uploads/MSDS/10098.pdf
Regulatory For research purposes only
Related Products Recommended Secondary Antibodies
Goat Anti Mouse IgG (STAR76...) RPE Goat Anti Mouse IgG IgA IgM (STAR87...) Alk. Phos., HRP Rabbit Anti Mouse IgG (STAR9...) FITC
Page 2 of 3 Goat Anti Mouse IgG (STAR77...) HRP Rabbit Anti Mouse IgG (STAR12...) RPE Goat Anti Mouse IgG (Fc) (STAR120...) FITC, HRP Rabbit Anti Mouse IgG (STAR8...) DyLight®800 Goat Anti Mouse IgG (STAR70...) FITC Human Anti Mouse IgG2a (HCA037...) FITC, HRP Rabbit Anti Mouse IgG (STAR13...) HRP Goat Anti Mouse IgG (H/L) (STAR117...) Alk. Phos., DyLight®488, DyLight®549, DyLight®649, DyLight®680, DyLight®800, FITC, HRP Recommended Negative Controls
MOUSE IgG2a NEGATIVE CONTROL (MCA929)
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'M317939:180717'
Printed on 01 Aug 2018
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