GTF2H2 Antibody Cat. No.: 27-253

GTF2H2 Antibody

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human GTF2H2.

TESTED APPLICATIONS: ELISA, WB

GTF2H2 antibody can be used for detection of GTF2H2 by ELISA at 1:62500. GTF2H2 APPLICATIONS: antibody can be used for detection of GTF2H2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate

PREDICTED MOLECULAR 44 kDa WEIGHT:

Properties

PURIFICATION: Antibody is purified by peptide affinity chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

September 24, 2021 1 https://www.prosci-inc.com/gtf2h2-antibody-27-253.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: GTF2H2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: GTF2H2

ALTERNATE NAMES: GTF2H2, BTF2, BTF2P44, MGC102806, T-BTF2P44, TFIIH, p44

ACCESSION NO.: NP_001506

PROTEIN GI NO.: 6681762

GENE ID: 2966

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

GTF2H2 is part of a 500 kb inverted duplication on 5q13. This duplicated region contains at least four and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. GTF2H2 is the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene.This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it BACKGROUND: prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-15 BM083743.1 1-15 16-1121 AF078847.1 1-1106 1122-1537 BG283896.1 128-543 1538-1951 AC044797.5 132110-132523 c

September 24, 2021 2 https://www.prosci-inc.com/gtf2h2-antibody-27-253.html REFERENCES: 1) Ren, P., (2007) Am. J. Respir. Crit. Care Med. 175 (11), 1151-1157.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 24, 2021 3 https://www.prosci-inc.com/gtf2h2-antibody-27-253.html