SANTA CRUZ BIOTECHNOLOGY, INC. RBM33 shRNA (h) Lentiviral Particles: sc-89462-V

BACKGROUND APPLICATIONS containing RNA recognition motifs, including various hnRNP proteins, RBM33 shRNA (h) Lentiviral Particles is recommended for the inhibition of are implicated in the regulation of alternative splicing and components RBM33 expression in human cells. of snRNPs. The RBM (RNA-binding motif) family encodes proteins with an RNA binding motif that have been suggested to play a role in the modula- SUPPORT REAGENTS tion of apoptosis. RBM33 (RNA-binding motif protein 33) is a 1,170 amino Control shRNA Lentiviral Particles: sc-108080. Available as 200 µl frozen acid protein that contains one RRM (RNA recognition motif) domain. Existing viral stock containing 1.0 x 106 infectious units of virus (IFU); contains an as two alternatively splice isoforms, the RBM33 gene is conserved in chim- shRNA construct encoding a scrambled sequence that will not lead to the panzee, cow, mouse, rat, chicken and zebrafish, and maps to human chromo- specific degradation of any known cellular mRNA. some 7q36.3. 7 is about 158 million bases long, encodes over 1000 and makes up about 5% of the . The deletion of RT-PCR REAGENTS a portion of the q arm of is associated with Williams-Beuren syndrome, a condition characterized by mild mental retardation, an unusual Semi-quantitative RT-PCR may be performed to monitor RBM33 gene expres- comfort and friendliness with strangers and an elfin appearance. Deletions of sion knockdown using RT-PCR Primer: RBM33 (h)-PR: sc-89462-PR (20 µl). portions of the q arm of chromosome 7 are also seen in a number of myeloid Annealing temperature for the primers should be 55-60° C and the extension disorders including cases of acute myelogenous leukemia and myelodysplasia. temperature should be 68-72° C.

REFERENCES BIOSAFETY 1. Varani, G., et al. 1998. RNA recognition by RNP proteins during RNA pro- Lentiviral particles can be employed in standard Biosafety Level 2 tissue cessing. Annu. Rev. Biophys. Biomol. Struct. 27: 407-445. culture facilities (and should be treated with the same level of caution as with any other potentially infectious reagent). Lentiviral particles are repli- 2. Hillier, L.W., et al. 2003. The DNA sequence of human chromosome 7. cation-incompetent and are designed to self-inactivate after transduction Nature 424: 157-164. and integration of shRNA constructs into genomic DNA of target cells. 3. Scherer, S.W., et al. 2003. Human chromosome 7: DNA sequence and biology. Science 300: 767-772. STORAGE 4. Maris, C., et al. 2005. The RNA recognition motif, a plastic RNA-binding Store lentiviral particles at -80º C. Stable for at least one year from the platform to regulate post-transcriptional gene expression. FEBS J. 272: date of shipment. Once thawed, particles can be stored at 4º C for up to 2118-2131. one week. Avoid repeated freeze thaw cycles. 5. Sutherland, L.C., et al. 2005. RNA binding motif (RBM) proteins: a novel RESEARCH USE family of apoptosis modulators? J. Cell. Biochem. 94: 5-24. The purchase of this product conveys to the buyer the nontransferable right 6. Eckert, M.A., et al. 2006. The neurobiology of Williams syndrome: cascading to use the purchased amount of the product and all replicates and derivatives influences of visual system impairment? Cell. Mol. Life Sci. 63: 1867-1875. for research purposes conducted by the buyer in his laboratory only (whether 7. Fukuda, T., et al. 2009. hnRNP K interacts with RNA binding motif pro- the buyer is an academic or for-profit entity). The buyer cannot sell or other- tein 42 and functions in the maintenance of cellular ATP level during wise transfer (a) this product (b) its components or (c) materials made using stress conditions. Genes Cells 14: 113-128. this product or its components to a third party, or otherwise use this product or its components or materials made using this product or its components CHROMOSOMAL LOCATION for Commercial Purposes. Genetic locus: RBM33 (human) mapping to 7q36.3. PROTOCOLS PRODUCT See our web site at www.scbt.com for detailed protocols and support products. RBM33 shRNA (h) Lentiviral Particles is a pool of concentrated, transduction- ready viral particles containing 3 target-specific constructs that encode 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 200 µl frozen stock containing 1.0 x 106 infectious units of virus (IFU) in Dulbecco’s Modified Eagle’s Medium with 25 mM HEPES pH 7.3. Suitable for 10-20 transductions. Also see RBM33 siRNA (h): sc-89462 and RBM33 shRNA Plasmid (h): sc-89462-SH as alternate gene silencing products.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com