New Records of Non-Biting Midges (Diptera: Chironomidae) From

ZOOSYSTEMATICA ROSSICA, 23(1): 150–157 25 JUNE 2014

New records of non-biting midges (Diptera: Chironomidae) from springs and streams of the Ukrainian Carpathians (Gorgany Massif) Новые находки хирономид (Diptera: Chironomidae) из родников и ручьев Украинских Карпат (массив Горганы)

V.A. BARANOV & A.A. PRZHIBORO*

В.А. БАРАНОВ, А.А. ПРЖИБОРО

V.A. Baranov, I.I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, 15 B. Khmelnytskogo, 01601 Kiev, Ukraine; Leibniz Institute for Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany. E-mail: [email protected] A.A. Przhiboro, Zoological Institute, Russian Academy of Sciences, 1 Universitetskaya Emb., St Petersburg 199034, Russia. E-mail: [email protected] *Corresponding author.

Seven species of Chironomidae (Diptera) are recorded from Ukraine for the first time: Kreno- pelopia binotata (Wiedemann, 1817), Chaetocladius gracilis Brundin, 1956, Limnophyes asquamatus Andersen, 1937, Paraphaenocladius exagitans monticola Strenzke, 1950, Thienemannia gracei (Edwards, 1929), T. gracilis Kieffer, 1909, and Micropsectra notescens (Walker, 1856). The record of C. laminatus Brundin, 1947 from Ukraine is confirmed. Adults of all species emerged from semiaquatic substrata (moss, litter) collected from mountain springs and streams in the Gorgany Massif of the Ukrainian Carpathians; C. gracilis, P. exagitans monticola and T. gracei are for the first time recorded from springs. The type specimens of C. gracilis are reexamined, and the lectotype is designated. Emendations are proposed to the diagnosis of the genus Thienemannia Kieffer, 1911 and to the diagnostic characters of the male of T. gracei. Семь видов хирономид (Diptera: Chironomidae) впервые отмечены с территории Украины: Krenopelopia binotata (Wiedemann, 1817), Chaetocladius gracilis Brundin, 1956, Limnophyes asquamatus Andersen, 1937, Paraphaenocladius exagitans monticola Strenzke, 1950, Thienemannia gracei (Edwards, 1929), T. gracilis Kieffer, 1909 и Micropsectra notescens (Walker, 1856). Подтверждена находка C. laminatus Brundin, 1947 на Украине. Имаго всех видов были выведены из полуводных субстратов (мхи, листовой опад), которые были собраны из горных родников и ручьев массива Горганы (Украинские Карпаты); C. gracilis, P. exagitans monticola и T. gracei впервые отмечены из родников. Переисследован типовой материал C. gracilis; обозначен лектотип этого вида. Предложены исправления диагноза рода Thienemannia Kieffer, 1911 и диагностических признаков самца T. gracei. Key words: non-biting midges, distribution, diagnostic characters, larval habitats, springs, streams, Ukraine, Carpathians, Diptera, Chironomidae, Chaetocladius, Thienemannia, new records Ключевые слова: хирономиды, распространение, диагностические признаки, биотопы личинок, родники, ручьи, Украина, Карпаты, Diptera, Chironomidae, Chaetocladius, Thien- emannia, новые находки

INTRODUCTION small running waters, especially springs and hygropetric habitats, are almost unstudied Non-biting midges (Diptera: Chiron- (Baranov, 2011). The Carpathian region is omidae) are among the most abundant mac- very interesting in biogeographical aspects roinvertebrates in aquatic and semiaquatic of aquatic fauna, taking into account its environments. In Ukraine, chironomids of connections with several other large Eu-

© 2014 Zoological Institute, Russian Academy of Scienсes V.A. BARANOV & A.A. PRZHIBORO. NEW RECORDS OF CHIRONOMIDAE FROM CARPATHIANS 151 ropean mountain systems (Polishchuk & River, 3 km NE of central part of Bystritsa- Gerasevich, 1986). Our study was focused Nadvirnyanska Vill., 710 m. Habitats: thick on the chironomids from semiaquatic habi- moss cushions on limestones and on wood. tats in the Gorgany Massif of the Ukrainian Site 5B: spring, a tributary of Studenyi Carpathians. Stream 200 m upstream of the confluence with Bystritsa River, same locality, 750 m. STUDY LOCALITIES AND HABITATS Habitats: same as at site 5A. A more detailed description of study The springs and streams studied are sites and their conditions will be provid- situated in the vicinity of Bystritsa-Nad- ed in a paper on chironomid assemblages virnyanska Village (48°27´N 24°15´E, (Przhiboro & Baranov, in preparation). ca. 30 km SW of Nadvirna Town, Ivano- Frankovsk Province of Ukraine). The study MATERIAL AND METHODS sites are situated in five localities in the ba- sins of Dzhurdzhii (=Derdinets) and Stude- All Carpathian chironomids considered nyi, two cold mountain streams (water tem- in this paper are adult specimens reared in perature 4–10 °C in the sampling period), the laboratory from semiaquatic substrates both being the tributaries of the Bystritsa collected on August 15–21, 2004. Rearing River. The material was taken at the shore- techniques were used as described by Przhi- lines, only from semiaquatic habitats repre- boro & Shamshev (2007) and Przhiboro & sented by two major substrate types, mosses Paasivirta (2011), but the temperature usu- and beech litter. Below, the study sites and ally did not exceed 15 °C. The Carpathian habitats are briefly characterized. material was collected by A. Przhiboro. The Site 1: a small unnamed shaded stream Chironomidae adults were determined in in forest (spruce, fir and beech predomi- permanent euparal slides or in temporary nant), tributary of Dzhurdzhii Stream in its water/glycerole slides. Morphological ter- middle course; ca. 4 km NEE of central part minology and measurements follow Sæther of Bystritsa-Nadvirnyanska Vill., ca. 850 m. (1980). The photographs were taken from Habitats: mosses on limestone gravel/sto- permanent slides with a Leica DFC320 nes, beech litter with rotten wood. digital camera on a Leica DM5000B mi- Site 2A: spring (rheocrene) at the shore croscope with Nomarski contrast. Series of of Dzhurdzhii Stream some hundred meters photos taken at different focal planes were of site 1; ca. 800 m. Habitat: mosses with stacked using Helicon Focus 5.1 and the re- Cardamine amara on limestones. sulting images were further enhanced using Site 2B: water margin of Dzhurdzhii Adobe Photoshop CS software. Most part Stream, same locality. Habitat: mosses on of the material is housed at the Zoological limestones. Institute, St Petersburg; some slides are Site 3: upper reach of Dzhurdzhii Stream kept in the collection of V. Baranov. (narrow canyon in spruce forest); ca. 4.5 km E of central part of Bystritsa-Nadvirnyan- RESULTS ska Vill., ca. 1300 m. Habitat: mosses on limestones. Among the chironomids collected, 19 Site 4: spring, head of Studenyi Stream species were recorded (Przhiboro & Ba- in beech forest; ca. 3 km NNE of central part ranov, in preparation). Seven species and of Bystritsa-Nadvirnyanska Vill., 1180 m. the genera Thienemannia Kieffer, 1911 and Habitats: mosses on limestone gravel/ Krenopelopia Fittkau, 1962 were recorded stones, beech litter with rotten wood. for the first time from Ukraine. An an- Site 5A: Studenyi Stream 100 m up- notated list of new records is given below. stream of the confluence with Bystritsa Diagnostic characters of five species are il-

© 2014 Zoological Institute, Russian Academy of Scienсes, Zoosystematica Rossica 23(1): 150–157 152 V.A. BARANOV & A.A. PRZHIBORO. NEW RECORDS OF CHIRONOMIDAE FROM CARPATHIANS lustrated in Figs 1–10. For the Carpathian types are kept at the Swedish Museum of Natu- localities, only the numbers of sites are giv- ral History in Stockholm. en (for details, see Material and Methods), Notes. Species diagnostics in Chaeto- as well as the dates of emergence of adults. cladius is problematic, and the genus needs The habitat (substrate) type is moss if not a revision. Chaetocladius gracilis was de- specified as litter. scribed from the males collected in two localities in northern Sweden. In the origi- Family CHIRONOMIDAE nal description, Brundin (1956: 124–125) listed one male from Riksgränsfjället and Subfamily TANYPODINAE numerous males from Vassijaure. Upon our Krenopelopia binotata request, Dr. Yngve Brodin kindly examined (Wiedemann, 1817) the collection of the Swedish Museum of Natural History and found two males from Material. Ukraine, Ivano-Frankovsk Prov.: the first locality and 37 ones, from the sec- 15 males, 17 females, site 4, 1.X.2004; site 2A, ond, with the Brundin’s identification la- site 4 (litter), site 5B, all 8.III.2005. Crimea: 1 bels “Chaetocladius gracilis” and other label larva, Yalta, Uchan-Su River, Polyana Skazok, 8.V.2011, V. Baranov leg. data corresponding to those in the original Notes. The species and the genus are description. All the material was kept in recorded for the first time from Ukraine. ethanol. In one male from the first locality, Krenopelopia binotata is widespread in west- the abdomen was separated and cleared. ern Europe and Siberia (Ashe & O’Connor, The second author examined both the ä ä 2009; Sæther & Spies, 2013). specimens from Riksgr nsfj llet and five The adults in our material are paler and best preserved specimens from Vassijaure, less contrastingly coloured as compared to and slide-mounted in euparal all specimens the published descriptions of K. binotata from Vassijaure and a specimen with sepa- ä ä (Goetghebuer, 1936; Fittkau, 1962; Lang- rated abdomen from Riksgr nsfj llet (some ton & Pinder, 2007) and to the specimens parts of one specimen from Vassijaure were from the collection of the Zoological Insti- slide-mounted in Berlese fluid). tute (collected by Chernovskii, 1938, Len- Brundin (1956) did not designate the ingrad Prov.). holotype, hence all the specimens should be considered as syntypes. The only specimen with separated and cleared abdomen Subfamily ORTHOCLADIINAE was probably used for drawing the hypo- Chaetocladius gracilis Brundin, 1956 pygium, according to the Brundin’s draw- (Figs 1–4) ing technique described in the same paper (Brundin, 1956: 14–15). This specimen fits Material. Ukraine, Ivano-Frankovsk Prov.: 1 male, site 3, 1.X.2004. well to the original description, so we des- Type material (reexamined). Lectotype (designate it as a lectotype. The hypopygium of ignated here). Male; “Schwedisch-Lappland, the lectotype (Fig. 4) slightly differs from Torneträskgebiet: am 9.9.1950 an der Nordbösc- the Brundin’s drawing (Brundin, 1949: 123, hung des Riksgränsfjället fliegend” [Sweden, Fig. 87) in the shape of the gonostyle but Lapplandia, area of Torneträsk, flying at northern the differences may be explained by the dif- ä ä slope of Riksgr nsfj llet Mountain near border ferent position of the gonostyles on the slide with Norway, 9 September 1950, Brundin leg.] and in ethanol. The paralectotypes mostly (no. NHRS-BYWS000000803). Paralectotypes. share the characters of the lectotype and fit Sweden: 1 male, same data as in lectotype (no. NHRS-BYWS000000804); 5 males, same area, to the original description, but differ from stream mouth at shore of Vassijaure Lake [near these in the inferior volsella, which looks Riksgränsfjället], 11 September 1950, Brundin wider and differently-shaped (Fig. 3). How- leg. (nno. NHRS-BYWS000000805-809). The ever, an examination of the specimens in

Figs 1–6. Chaetocladius spp., male hypopygia: 1, C. gracilis, specimen from Carpathians (left, dorsal part; right, ventral part); 2, same specimen, anal point; 3, C. gracilis, paralectotype from Vassijaure (Sweden); 4, C. gracilis, lectotype (Sweden); 5–6, C. laminatus, specimens from Carpathians.

Figs 7–10. 7, Paraphaenocladius exagitans monticola, male, hypopygium (left, dorsal part; right, ventral part); 8–9, Thienemannia gracei, male: 8, hypopygium (left, dorsal part; right, ventral part); 9, apical segments of antenna; 10, setation on eye of males of Thienemannia gracei (left) and T. gracilis (right).

© 2014 Zoological Institute, Russian Academy of Scienсes, Zoosystematica Rossica 23(1): 150–157 V.A. BARANOV & A.A. PRZHIBORO. NEW RECORDS OF CHIRONOMIDAE FROM CARPATHIANS 155 ethanol revealed that the differences mostly not illustrated their species and do not have resulted from the different position of the a photo of its hypopygium (E. Stur, pers. volsella on the slides. comm.). Based on the Brundin’s original The male in our material slightly dif- description (Brundin, 1947) and the brief fers from the Brundin’s original description diagnosis of “C. sp. 2” (Ekrem et al., 2010) in some characters of the gonostyle (outer we consider that our material fits well to C. margin with distal angle more pronounced laminatus (see Figs 5–6; in Fig. 5, anal point and inner margin with a rather strong seta is not horizontal and so it looks shorter). before the megaseta; Fig. 1), in the anal point without hairs except for the base (Fig. Limnophyes asquamatus Andersen, 1937 2), and in the number of setae on vein R (13 vs. “about 10”). However, the comparison Material. Ukraine, Ivano-Frankovsk Prov.: 1 of our specimen with the lectotype has re- male, site 4 (litter), 5.XI.2004. vealed that they are very similar in the de- Notes. The species is recorded for the tails of the gonostyle and differ only in the first time from Ukraine, widespread in Eu- hairs on the anal point. The lectotype has 10 rope (Ashe & O’Connor, 2012; Sæther & setae on R, but paralectotypes, 10–12 ones. Spies, 2013). Chaetocladius gracilis is recorded from Ukraine for the first time. It is a rare species Paraphaenocladius exagitans monticola previously known from Finland, France, Strenzke, 1950 Norway, Novaya Zemlya, Romania and (Fig. 7) Sweden (Ashe & O’Connor, 2012; Sæther & Spies, 2013). The species is recorded for Material. Ukraine, Ivano-Frankovsk Prov.: 2 the first time from springs (Lindegaard, males, site 1, 8.III.2005; 5 males, site 4 (litter), 8.III.2005; 1 male, site 5B, 9.IX.2004. 1995; Paasivirta, 2007). Notes. The subspecies and species are recorded for the first time from eastern Chaetocladius laminatus Brundin, 1947 Europe; it was known from eight countries (Figs 5–6) in western and northern Europe (Ashe & Material. Ukraine, Ivano-Frankovsk Prov.: 1 O’Connor, 2012; Sæther & Spies, 2013). female, site 1, 1.X.2004; 4 males, 1 female, site The species is recorded for the first time 2A, 1.X.2004; 1 female, site 2B, 9.IX.2004; 5 from springs (Lindegaard, 1995). males, 24 females, site 3, 1.X.2004; 1 female, site 4, 1.X.2004; 1 female, site 5A, 1.X.2004; 2 females, site 5B, 9.IX.2004. Thienemannia gracei (Edwards, 1929) Notes. The occurrence of Chaetocladius (Figs 8–10) laminatus in Ukraine is confirmed. The Material. Ukraine, Ivano-Frankovsk Prov.: 5 previous record (Baranov, 2011) was based males, site 1, 7.IX.2004; 1 male, site 1, 5.XI.2004; on the larvae only, and thus could be con- 9 males, site 1 (litter), 1.X.2004; 4 males, site 2B, sidered as unreliable, due to difficulties 9.IX.2004; 1 male, site 3, 9.IX.2004; 1 male, site in distinguishing larvae in the Chaetocla- 4, 9.IX.2004; 1 male, 2 females, site 4 (litter), dius dentiforceps-aggregate (Moller Pil- 1.X.2004; 1 male, site 5A, 7.IX.2004; 2 males, site lot, 2013). The species is widespread in the 5B, 9.IX.2004; 3 males, site 5B, 1.X.2004. Western Palaearctic (Ashe & O’Connor, Notes. The species and the genus are re- 2012; Sæther & Spies, 2013). corded for the first time from Ukraine. The Ekrem et al. (2010) recorded from Nor- species was previously recorded from eight way a very similar undescribed species European countries, Lebanon and China (“Chaetocladius sp. 2”) differing from C. (Ashe & O’Connor, 2012; Sæther & Spies, laminatus in fine details of the hypopygium 2013). The species is recorded for the first and COI sequences. However, they have time from springs (Lindegaard, 1995).

The males in our material correspond Notes. The species is recorded for the well to the redescription of T. gracei pro- first time from Ukraine, widespread in vided by Sæther (1985) in the structure of the Western Palaearctic (Sæther & Spies, the hypopygium (Fig. 8), the anteprono- 2013). tum and wing chaetotaxy. At the same time, our specimens differ from the redescription ACKNOWLEDGEMENTS in the lower AR (0.35–0.40; n=6), smaller wing length (1.10–1.47 mm; n=6) and lower A. Przhiboro is grateful to Andrey Susulo- number of setae on squama (4–6; n=6). Both vsky (Lviv) and the administration of the Gor- gany Nature Reserve for the substantial assis- males examined by Sæther (1985) were col- tance during the field work in the Carpathians in lected in the Great Britain. We suppose 2004. The research of V. Baranov was supported that the above three characters are variable; by a grant for young scientists from the Russian hence, they may be used for the identifica- Foundation for Basic Research, Ref. no. 13-04- tion of T. gracei only with reservations. 90918 mol_in_nr. The research of A. Przhiboro In all males of T. gracei in our material, was partially supported by a grant from the Rus- the eyes are pubescent (with microtrichia sian Scientific Foundation, Ref. no. 14-14-01134. slightly shorter than the height of omma- Both authors are grateful to Yngve Brodin tidial lenses) only at the lower margin (Fig. (Stockholm) for loaning the types of Chaetocla- dius gracilis and additional information on these, 10). We could not see hairs throughout the to Martin Spies (München), Joel Moubayed- eye surface. Moubayed-Breil described a Breil (Montpellier) and Elisabeth Stur (Trond- similar arrangement of hairs for T. fulvo- heim) for valuable comments and discussion on fasciata (Kieffer, 1921) (Moubayed-Breil, different issues of Chironomidae taxonomy, and 2013: 81, Fig. 3). Hairs or pubescence on to Henk Moller Pillot (Tilburg) for reviewing eyes were considered one of the diagnos- the manuscript. tic characters of the genus Thienemannia æ (S ther, 1985: 112–113; Cranston et al., REFERENCES 1989: 244). We propose the following emen- dation to the diagnosis of the genus: Eyes Ashe P. & O’Connor J.P. 2009. A World cata- hairy or pubescent over the surface or only logue of Chironomidae (Diptera). Part 1. Bu- at lower margin. cho nomyiinae, Chilenomyiinae, Podonominae, Aphroteniinae, Tanypodinae, Usambaromyi- inae, Diamesinae, Prodiamesinae and Telmat- Thienemannia gracilis Kieffer, 1909 ogetoninae. Dublin: Irish Biogeographical (Fig. 10) So ciety. 445 p. Ashe P. & O’Connor J.P. 2012. A World cata- Material. Ukraine, Ivano-Frankovsk Prov.: 2 logue of Chironomidae (Diptera). Part 2. Or- males, site 1, 7.IX.2004; 1 male, site 4, 9.IX.2004; thocladiinae. Dublin: Irish Biogeographical 5 males, site 5A, 1.X.2004. Society. xvi + 968 p. Notes. The species is recorded for the Baranov V.A. 2011. A preliminary annotated first time from Ukraine, widespread in Eu- checklist of non-biting midges (Diptera, rope (Ashe & O’Connor, 2012; Sæther & Chironomidae) of Ukraine. Ukrainska Ento- Spies, 2013). mofaunistyka, 2(1): 7–24. (In Ukrainian). Brundin L. 1956. Zur Systematik der Ortho- cladiinae (Dipt. Chironomidae). Institute of Subfamily CHIRONOMINAE Freshwater Research, Drottningholm, Report 37: 5–185. Tribe TANYTARSINI Cranston P.S., Oliver D.R. & Sæther O.A. Micropsectra notescens (Walker, 1856) 1989. The adult males of Orthocladiinae (Diptera: Chironomidae) of the Holarctic Material. Ukraine, Ivano-Frankovsk Prov.: 5 region – Keys and diagnoses. In: Wieder- males, 3 females, site 2A, 8.III.2005. holm T. (Ed.). The adult males of Chironomi-

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