Fold Adjusted Gene_Name UniProtKB_Accession Change P value lipocalin 2 18.04 3.10E-05 P11672 /// Q3UE34 /// Q60842 6B 10.93 0.004548 Q9Z331 /// Q05B38 /// Q0VDR7 interleukin 12b 9.58 7.60E-05 P43432 /// Q3ZAX5 formyl peptide receptor 1 8.65 6.90E-05 P33766 /// Q3UV01 8.35 0.000825 P04104 7.75 0.000869 Q922U2 /// Q32P04 /// Q9CRS6 nitric oxide synthase 2, inducible, macrophage 7.41 8.40E-05 P29477 interleukin 1 alpha 7.04 2.00E-06 P01582 /// Q3U0Y6 /// Q62161 serum amyloid A 3 6.83 0.000116 P04918 C-type lectin domain family 4, member e 6.36 7.60E-05 Q9R0Q8 /// Q3U271 /// Q4FK29 interleukin 1 receptor antagonist 6.24 3.90E-05 P25085 /// A2AIU1 /// Q3TBV5 chemokine (C-C motif) ligand 3 6.21 2.00E-06 P10855 /// Q3U6F9 /// Q5QNW0 6.05 0.000869 Q61781 /// Q61782 /// Q99LE0 5.69 0.001499 Q9Z2K1 /// Q3SYP5 /// Q3ZAW8 interleukin 10 5.54 0.000779 P18893 /// Q0VBJ1 /// Q3U879 TNFAIP3 interacting 3 5.41 2.00E-06 A5HU64 /// Q3MI11 /// Q3SXA6 keratinocyte differentiation associated protein 5.28 1.30E-05 Q3V2T4 5.11 0.005067 P50446 /// Q9CV13 desmoglein 1 beta 5.09 0.00039 Q7TSF1 /// B2RQH0 /// Q9CUH7 HtrA serine peptidase 4 4.92 0.000499 A2RT60 desmocollin 3 4.89 0.000701 P55850 interleukin 1 beta 4.85 5.10E-05 P10749 /// Q2M4J6 /// Q3TI17 stefin A3 4.76 0.012138 P35175 /// Q540I0 small proline-rich protein 2A 4.68 0.037384 Q9CQK8 /// Q9CQK8 /// Q4KL71 S100 calcium binding protein A8 (calgranulin A) 4.37 0.001262 P27005 /// Q53X15 RIKEN cDNA 9930022N03 gene 4.36 0.000152 --- chemokine (C-C motif) ligand 4 4.34 2.00E-06 P14097 /// Q5QNV9 CD14 antigen 4.33 9.90E-05 P10810 /// Q3TE16 /// Q3U6A3 4.14 0.004159 Q9QWL7 4.13 0.000829 P02535 /// A2A513 4.05 0.005937 P11088 /// P11088 /// Q61570 3.92 0.000825 Q8BP77 /// Q9CV95 /// Q9QZX5 chemokine (C-X-C motif) ligand 2 3.84 0.003817 P10889 /// Q3U1J5 /// Q3U1L9 immunoresponsive gene 1 3.75 0.001118 P54987 interleukin 1 family, member 9 3.68 0.000829 Q8R460 /// Q14BB6 /// Q3U0P4 G protein-coupled receptor 109A 3.59 0.000193 Q9EP66 /// Q0VBA6 serine (or cysteine) peptidase inhibitor, clade B, member 2 3.47 0.018225 P12388 /// Q542A3 6-phosphofructo-2-kinase/fructose-2,6- biphosphatase 3 3.46 0.000184 A2AUP1 /// A2AUP3 /// A2AUP4 lipase, endothelial 3.34 0.003456 Q9WVG5 /// Q3TA44 /// Q3U5K0 dual specificity phosphatase 1 3.33 5.10E-05 P28563 /// Q3U8K3 /// Q3UCQ6 aspartic peptidase, retroviral-like 1 3.33 0.001207 Q09PK2 small proline-rich protein 1A 3.30 0.005936 Q62266 /// Q3UMN4 serine (or cysteine) peptidase inhibitor, clade B, member 5 3.28 5.30E-05 P70124 /// Q3ULZ0 interleukin 8 receptor, beta 3.11 0.001701 P35343 /// Q53X27 histidine decarboxylase 3.06 0.000379 P23738 /// Q54AE6 /// Q7TMW5 predicted gene, EG622976 3.05 0.001501 A2ALN9 /// Q8BHV4 G protein-coupled receptor 84 3.05 0.003205 Q8CIM5 SLAM family member 7 3.05 0.001601 Q8BHK6 /// Q18PG4 /// Q18PI2 arginase type II 3.03 0.001204 O08691 /// Q8R1Q7 solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 3.01 2.00E-06 Q9WTR6 /// Q3TN05 /// Q542C8 PERP, TP53 apoptosis effector 2.96 0.001093 Q9JK95 tripartite motif-containing 29 2.93 0.000825 Q8R2Q0 dermokine 2.93 0.000651 Q6P253 lymphocyte antigen 6 complex, locus D 2.88 0.015713 P35459 interferon activated gene 205 2.84 0.000651 Q8CGE8 solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3 2.84 0.000115 Q91Y63 /// A2A4U9 /// Q3UUJ6 CD38 antigen 2.80 0.007108 P56528 /// Q3UCS6 /// Q4FJL8 dual specificity phosphatase 2 2.79 7.60E-05 Q05922 /// Q2M4K0 /// Q80ZN1 chemokine (C-X-C motif) ligand 3 2.76 0.001981 Q3UNK9 /// Q6W5C0 desmoglein 3 2.74 0.001262 O35902 /// Q3UFC6 small proline-rich protein 2A 2.74 0.074454 Q9CQK8 /// Q9CQK8 /// Q4KL71 small proline-rich protein 2A 2.74 0.074454 Q9CQK8 /// Q9CQK8 /// Q4KL71 S100 calcium binding protein A9 (calgranulin B) 2.74 0.003085 P31725 /// Q3UP42 superoxide dismutase 2, mitochondrial 2.72 7.60E-05 P09671 /// Q3TJA2 /// Q3U8W4 tumor necrosis factor receptor superfamily, member 9 2.71 0.000701 P20334 /// A2A886 /// A2A887 endothelin 1 2.68 0.002456 P22387 /// Q544E0 growth arrest and DNA-damage-inducible 45 beta 2.66 9.30E-05 P22339 formyl peptide receptor 2 2.62 7.60E-05 O88536 gap junction protein, beta 2 2.61 0.034389 Q00977 /// Q3TZE5 /// Q3UJE9 chemokine (C-C motif) receptor-like 2 2.60 0.000828 O35457 calmodulin 5 2.56 0.001426 Q9JM83 /// Q9JM83 /// Q6W3E0 chloride channel calcium activated 2 2.55 0.002373 Q3UZ16 /// Q8C9E1 /// Q9EQR4 CD274 antigen 2.54 0.003264 Q9EP73 /// Q3U472 matrix metallopeptidase 13 2.53 0.000492 P33435 /// Q3U2N6 /// Q3U5R7 suppressor of cytokine signaling 3 2.52 1.70E-05 O35718 /// Q3U9J8 late cornified envelope 1C 2.49 0.007839 Q9D1C5 tumor necrosis factor, alpha-induced protein 3 2.48 0.000651 Q60769 /// Q3T9G9 /// Q3TBB9 C-type lectin domain family 4, member d 2.45 0.001597 Q9Z2H6 /// Q3UBK0 /// Q4KL29 small proline-rich protein 1B 2.45 0.141887 Q62267 /// Q0VEC0 glucosaminyl (N-acetyl) transferase 2, I-branching enzyme 2.44 0.001345 P97402 /// A2IQH4 /// A2IQH5 cDNA sequence BC100530 2.43 0.012225 Q497J0 interferon induced transmembrane protein 1 2.41 0.006212 Q8R2S7 /// Q9D103 solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 2.41 0.011599 O88627 /// Q7TMQ0 /// Q8BJP1 myxovirus (influenza virus) resistance 2 2.41 0.00082 Q9WVP9 /// Q61924 /// Q61925 CD40 antigen 2.40 0.000651 P27512 /// A2A5L5 /// A2A5L7 keratin 77 2.36 0.017146 Q6IFZ6 /// Q08EK4 /// Q08EK5 CD69 antigen 2.36 0.000876 P37217 /// Q3U6A8 desmocollin 1 2.35 0.017585 P55849 /// Q2VPA9 /// Q32ME9 kallikrein related-peptidase 7 (chymotryptic, stratum corneum) 2.35 0.012316 Q91VE3 aquaporin 3 2.34 0.016106 Q8R2N1 /// Q3TU75 Dmx-like 2 2.33 0.001597 Q8BPN8 /// Q05CM4 annexin A8 2.32 0.000319 O35640 /// Q8K2N9 /// Q921D0 Epstein-Barr virus induced gene 3 2.32 0.005209 O35228 /// Q3U1K3 TNFAIP3 interacting protein 1 2.30 0.000651 Q9WUU8 /// Q3UXR9 /// Q6A0C8 keratin 79 2.28 0.021304 Q8VED5 solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 2.27 0.004915 P18581 /// Q38RA6 /// Q3TB99 expressed sequence AA467197 2.27 0.000825 Q810Q5 /// A2AK37 /// Q3UCF3 interleukin 7 receptor 2.26 0.005029 P16872 /// Q3U3Z0 /// Q5FW78 prostaglandin-endoperoxide synthase 2 2.25 0.000825 Q05769 /// Q3UMR6 /// Q543K3 killer cell lectin-like receptor subfamily B member 1B 2.25 0.004365 P27812 /// P27812 /// Q99JB4 predicted gene, 381484 2.25 0.002224 Q1AN92 granzyme G 2.24 0.314135 P13366 /// Q4KL28 interleukin-1 receptor-associated kinase 3 2.23 0.003825 Q8K4B2 /// Q0VB14 interleukin 6 2.21 0.003623 P08505 /// A2RTD1 /// Q0PMN1 pyrin and HIN domain family, member 1 2.21 0.010514 Q8BV49 haptoglobin 2.21 0.005628 Q61646 /// Q3UBS3 schlafen 4 2.20 0.000276 B2RWZ6 /// Q3UV10 /// Q3UV66 metallothionein 2 2.18 0.002257 P02798 pentraxin related gene 2.18 0.022516 P48759 adenosine A3 receptor 2.18 0.00022 Q61618 /// Q3U4C5 /// Q497R5 Fas (TNF receptor superfamily member 6) 2.17 0.001426 P25446 /// A1Y9B2 /// Q6GT31 mitogen-activated protein kinase kinase kinase 8 2.16 0.00073 Q07174 /// Q3T9M0 /// Q3U086 heat shock protein 1B 2.15 0.251439 P17879 /// Q61698 colony stimulating factor 3 receptor (granulocyte) 2.14 0.002147 P40223 /// A2A8Y3 /// Q14AY3 MAX dimerization protein 1 2.14 0.000303 P50538 /// Q4FK19 /// Q8CB04 cDNA sequence BC094916 2.12 0.031006 Q504N7 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 2.12 0.011454 A0PJG6 /// A2NTK5 /// A2NTK6 gene model 885, (NCBI) 2.10 0.007008 Q3TB92 /// B2RTB7 RIKEN cDNA I830012O16 gene 2.09 0.008234 Q3UAB1 /// Q8BPC3 adenosine A2b receptor 2.08 0.000452 Q60614 /// Q8BK41 /// Q8BXI2 matrix metallopeptidase 19 2.08 0.001426 Q9JHI0 /// Q2KHP2 /// Q3UW97 tumor necrosis factor receptor superfamily, member 1b 2.07 0.001574 P25119 /// Q3U2A9 /// Q545P4 phosphoinositide-3-kinase, regulatory subunit 5, p101 2.07 0.005029 Q5SW28 /// A1A4T4 chemokine (C-X-C motif) ligand 11 2.07 0.004033 Q9JHH5 /// Q8R392 protein C receptor, endothelial 2.07 0.000256 Q64695 /// A2AUV5 /// Q4FK76 radical S-adenosyl methionine domain containing 2 2.06 0.000869 Q8CBB9 /// Q3UAK4 triggering receptor expressed on myeloid cells 1 2.06 0.000832 Q9JKE2 /// Q14DT9 Rho family GTPase 1 2.06 0.002423 Q8BLR7 guanylate binding protein 5 2.05 0.003575 Q8CFB4 /// Q8BMN7 /// Q8BU78 nuclear factor, interleukin 3, regulated 2.05 0.009582 O08750 /// O89065 premature ovarian failure 1B 2.03 0.003623 Q8K4L4 /// A2ANY1 /// Q8R4R8 interferon induced transmembrane protein 1 2.02 0.007447 Q8R2S7 /// Q9D103 ELOVL family member 7, elongation of long chain fatty acids (yeast) 2.02 0.001462 Q9D2Y9 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha 2.01 7.60E-05 Q9Z1E3 /// Q3U9W9 /// Q3UB40 granzyme F 2.01 0.323026 P08883 /// Q497Z7 inhibitor of kappaB kinase epsilon 2.01 0.002107 Q9R0T8 /// Q3U6P9 /// Q5DU46 basic leucine zipper transcription factor, ATF-like 2.01 0.023772 O35284 /// A2RT86 RIKEN cDNA E430024C06 gene 2.00 0.098587 Q3UEQ2 RIKEN cDNA E430024C06 gene 2.00 0.098587 Q3UEQ2 CD180 antigen -2.00 0.000256 Q62192 /// Q3U0U7 /// Q8C251 G-protein coupled receptor 65 -2.00 0.002515 Q61038 /// Q3UQ35 /// Q3UZ41 NLR family, pyrin domain containing 1C -2.03 0.009381 Q2LKU8 regulator of G-protein signaling 2 -2.10 0.002515 O08849 chemokine (C-X3-C) receptor 1 -2.32 0.000869 Q9Z0D9 /// Q543X3 /// Q8BR50 leukocyte-associated Ig-like receptor 1 -2.45 0.003362 Q8BG84

Supplemental Table 1. Genes upregulated or downregulated in tumors by >2fold following CpG 1826 injection. DNA microarray analysis was performed on established MC38 tumors 6 hours after intratumoral injection of CpG 1826. Fold induction of gene expression of CpG-treated tumors relative to PBS-treated tumors is listed