Supplementary table-2
Pin1-WW bound phosphoproteins which showed at least five times intensity enhancement from FSK/IBMX treated HeLa cell lysates:
Phospho- Gene names UniProtKB Protein names Sequence window site position
Apoptotic chromatin condensation inducer in ACIN1 Q9UKV3 561 KEGRRASHTLLPS the nucleus Apoptotic chromatin condensation inducer in ACIN1 Q9UKV3 563 GRRASHTLLPSHR the nucleus ATN1 P54259 Atrophin-1 653 GAYKTATPPGYKP
ATN1 P54259 Atrophin-1 661 PGYKPGSPPSFRT
ATXN2 Q99700 Ataxin-2 667 PPVARTSPSGGTW
ATXN2 Q99700 Ataxin-2 684 SGVPRLSPKTHRP
ATXN2 Q99700 Ataxin-2 758 QDQRQNSPAGNKE
CBR1 P16152 Carbonyl reductase [NADPH] 1 2 _____MSSGIHVA
CBR1 P16152 Carbonyl reductase [NADPH] 1 3 ____MSSGIHVAL
CDC27 P30260 Cell division cycle protein 27 homolog 432 QPNINDSLEITKL Chromosome alignment-maintaining CHAMP1 Q96JM3 204 KLAPVPSPEPQKP phosphoprotein 1 Chromosome alignment-maintaining CHAMP1 Q96JM3 214 QKPAPVSPESVKA phosphoprotein 1 Chromosome alignment-maintaining CHAMP1 Q96JM3 382 SPSSWKSPPASPE phosphoprotein 1 Chromosome alignment-maintaining CHAMP1 Q96JM3 405 KTAPTLSPEHWKA phosphoprotein 1 Chromosome alignment-maintaining CHAMP1 Q96JM3 459 PDQRKTSPASLDF phosphoprotein 1 Chromosome alignment-maintaining CHAMP1 Q96JM3 603 QEELLASPKKLLE phosphoprotein 1 IRS2 Q9Y4H2 Insulin receptor substrate 2 306 FRPRSKSQSSGSS
IRS2 Q9Y4H2 Insulin receptor substrate 2 560 RSYRRVSGDAAQD
IRS2 Q9Y4H2 Insulin receptor substrate 2 594 PQPSSASLDEYTL
KIAA1429 Q69YN4 Protein virilizer homolog 1578 SFLSEPSSPGRTK
KIAA1429 Q69YN4 Protein virilizer homolog 1579 FLSEPSSPGRTKT Mediator of RNA polymerase II transcription MDC1 Q14676 1057 TPPGVATPPIPKI subunit 1 Mediator of DNA damage checkpoint protein MDC1 Q14676 1608 NRSSVKTPEPVVP 1 Mediator of RNA polymerase II transcription MED24 F5GY88 892 DANILSSPTDRSM subunit 24 MKI67 P46013 Antigen KI-67 579 AQSLVISPPAPSP
MKI67 P46013 Antigen KI-67 584 ISPPAPSPRKTPV
MKI67 P46013 Antigen KI-67 1071 IRTFKESPKQILD
MKI67 P46013 Antigen KI-67 1329 TENLTGSKRRPQT Phospho- Gene names UniProtKB Protein names Sequence window site position
MKI67 P46013 Antigen KI-67 2223 TKIACRSPQPDPV
MKI67 P46013 Antigen KI-67 2231 QPDPVGTPTIFKP
NIPBL Q6KC79 Nipped-B-like protein 2658 SLLGGGSPKNNTA
NPM1 P06748 Nucleophosmin 4 ___MEDSMDMDMS
NPM1 P06748 Nucleophosmin 125 VEEDAESEDEEEE Nuclear fragile X mental retardation- NUFIP2 Q7Z417 571 YELEKRTSPQVLG interacting protein 2 Nuclear fragile X mental retardation- NUFIP2 Q7Z417 572 ELEKRTSPQVLGS interacting protein 2 NUP107 P57740 Nuclear pore complex protein Nup107 10 SGFGEISSPVIRE
NUP35 Q8NFH5 Nucleoporin NUP53 73 PLLAGGSPPQPVV
NUP35 Q8NFH5 Nucleoporin NUP53 265 SPSLAFTPPIKTL
RANBP2 P49792 E3 SUMO-protein ligase RanBP2 1144 GKSVFGTPTLETA
RANBP2 P49792 E3 SUMO-protein ligase RanBP2 1509 QNPRKQSLPATSI
RANBP2 P49792 E3 SUMO-protein ligase RanBP2 2805 ITKSISSPSVSSE
RPS3 P23396 40S ribosomal protein S3 237 DEILPTTPISEQK
SEC16A J3KNL6 Protein transport protein Sec16A 2083 QPPLSLSPAPETK
SYNPO Q8N3V7 Synaptopodin 833 VSPRAASPAKPSS
TCOF1 E7ETY2 Treacle protein 153 TVANLLSGKSPRK
TCOF1 E7ETY2 Treacle protein 156 NLLSGKSPRKSAE Thyroid hormone receptor-associated protein THRAP3 Q9Y2W1 248 LSPRERSPALKSP 3 Thyroid hormone receptor-associated protein THRAP3 Q9Y2W1 253 RSPALKSPLQSVV 3 TICRR Q7Z2Z1 Treslin 838 RRSVSGSPESDEL
TLE3 H0YL70 Transducin-like enhancer protein 3 296 KKDAPTSPASVAS
TNKS1BP1 Q9C0C2 182 kDa tankyrase-1-binding protein 893 EFGKRDSLGAYAS
ZNF768 Q9H5H4 Zinc finger protein 768 83 PRFEPESPGFESR CHEK2-FHA bound phosphoproteins which showed at least five times intensity enhancement from FSK/IBMX treated HeLa cell lysates:
Phospho- Gene names UniProtKB Protein names Sequence window site position
Angiogenic factor with G patch and FHA AGGF1 Q8N302 7 MASEAPSPPRSPP domains 1 Angiogenic factor with G patch and FHA AGGF1 Q8N302 11 APSPPRSPPPPTS domains 1 AHCTF1 Q8WYP5 Protein ELYS 2261 LISPLASPADGVK
ANAPC1 Q9H1A4 Anaphase-promoting complex subunit 1 362 PALGVHSFSGVQR
APOB P04114 Apolipoprotein B-100 1949 SHDYKGSTSHHLV
APOB P04114 Apolipoprotein B-100 1950 HDYKGSTSHHLVS
ARHGEF7 Q14155 Rho guanine nucleotide exchange factor 7 645 SSSRKESAPQVLL
ATRX P46100 Transcriptional regulator ATRX 1527 IEIEDASPTKCPI
ATXN2L Q8WWM7 Ataxin-2-like protein 594 TSEPMGSPVSSKT
CBR1 P16152 Carbonyl reductase [NADPH] 1 2 _____MSSGIHVA
CBR1 P16152 Carbonyl reductase [NADPH] 1 3 ____MSSGIHVAL
CCNL1 Q9UK58 Cyclin-L1 335 STLGGFSPASKPS
CCNL1 Q9UK58 Cyclin-L1 341 SPASKPSSPREVK
CCNL2 Q96S94 Cyclin-L2 327 QVLDGTSGFSPAP
CDC27 P30260 Cell division cycle protein 27 homolog 432 QPNINDSLEITKL
CDK11A Q9UQ88 Cyclin-dependent kinase 11A 577 LAREYGSPLKAYT
CDK11A Q9UQ88 Cyclin-dependent kinase 11A 583 SPLKAYTPVVVTQ
CDK11B P21127 Cyclin-dependent kinase 11B 47 RDSKRDSLEEGEL
CDK11B P21127 Cyclin-dependent kinase 11B 285;273 LQDISDSERKTSS
CEP170 Q5SW79 Centrosomal protein of 170 kDa 354 ERTEEDSKSIKSD
CEP170 Q5SW79 Centrosomal protein of 170 kDa 356 TEEDSKSIKSDVP
CEP170 Q5SW79 Centrosomal protein of 170 kDa 881 IRERSESLDPDSS
CEP170 Q5SW79 Centrosomal protein of 170 kDa 1522 PPKQKSSPVNNHH
CEP170 Q5SW79 Centrosomal protein of 170 kDa 1529 PVNNHHSPGQTPT
CTNNB1 P35222 Catenin beta-1 551 QDTQRRTSMGGTQ
DAXX Q9UER7 Death domain-associated protein 6 739 IIVLSDSD_____
DSP P15924 Desmoplakin 2825 MSSAPGSRSGSRS
EIF5B O60841 Eukaryotic translation initiation factor 5B 214 PGPNIESGNEDDD
GRAMD3 Q96HH9 GRAM domain-containing protein 3 267 DFNDEFSDLDGVV
HDAC1 Q13547 Histone deacetylase 1 393 DAIPEESGDEDED
KIF21A Q7Z4S6 Kinesin-like protein KIF21A 1663 LQDGQISDTGDLG
MADD Q8WXG6 MAP kinase-activating death domain protein 1237 LASSRGTLSDSEI
MCM2 P49736 DNA replication licensing factor MCM2 139 RGLLYDSDEEDEE Mediator of DNA damage checkpoint protein MDC1 Q14676 168 LLLAEDSEEEVDF 1 Phospho- Gene names UniProtKB Protein names Sequence window site position
Mediator of DNA damage checkpoint protein MDC1 Q14676 1466 STDQPVTPEPTSQ 1 Mediator of DNA damage checkpoint protein MDC1 Q14676 176 EEVDFLSERRMVK 1 Mediator of DNA damage checkpoint protein MDC1 Q14676 1608 NRSSVKTPEPVVP 1 NCOR1 O75376 Nuclear receptor corepressor 1 2184 QRNDARSPGSISY
NCOR1 O75376 Nuclear receptor corepressor 1 1981 VISPASSPAPPQE
NCOR2 Q9Y618 Nuclear receptor corepressor 2 2065 EGVEPVSPVSSPS
NPM1 P06748 Nucleophosmin 70 AMNYEGSPIKVTL Nuclear fragile X mental retardation- NUFIP2 Q7Z417 652 GLERNDSWGSFDL interacting protein 2
PCM1 Q15154 Pericentriolar material 1 protein 1776 QEEDEESEGCPVS
cGMP-inhibited 3',5'-cyclic PDE3B Q13370 295 IRPRRRSSCVSLG phosphodiesterase B cGMP-inhibited 3',5'-cyclic PDE3B Q13370 296 RPRRRSSCVSLGE phosphodiesterase B PLK1 P53350 Serine/threonine-protein kinase PLK1 214 KKTLCGTPNYIAP
RBM39 Q14498 RNA-binding protein 39 136 PFRKDKSPVREPI
RPLP2 P05387 60S acidic ribosomal protein P2 102 DEKKEESEESDDD
RSL1D1 O76021 Ribosomal L1 domain-containing protein 1 358 KAQVKATNESEDE
RSL1D1 O76021 Ribosomal L1 domain-containing protein 1 361 VKATNESEDEIPQ C-Jun-amino-terminal kinase-interacting SPAG9 O60271 183 TKLHQLSGSDQLE protein 4 TCOF1 E7ETY2 Treacle protein 906 LAPAKESPRKGAA
TP53BP1 Q12888 Tumor suppressor p53-binding protein 1 776 SPRVDVSCEPLEG
TPR P12270 Nucleoprotein TPR 1187 PLNVSLSEEGKSQ
TRIM28 Q13263 Transcription intermediary factor 1-beta 19 ASAASGSPGPGEG
UBE2O Q9C0C9 Ubiquitin-conjugating enzyme E2 O 515 GTSRKKSIPLSIK
UBE2O Q9C0C9 Ubiquitin-conjugating enzyme E2 O 839 LTGSPTSPTVEPE
UBR4 Q5T4S7 E3 ubiquitin-protein ligase UBR4 178 DQKELASPVSPEL
UBR4 Q5T4S7 E3 ubiquitin-protein ligase UBR4 2724 SSPRSNTPMGDKD
UBR5 O95071 E3 ubiquitin-protein ligase UBR5 1728 QHQRAASTAPSST
UHRF1BP1 Q6BDS2 UHRF1-binding protein 1 1101 FFSMKRTVSQQSF
USP47 Q96K76 Ubiquitin carboxyl-terminal hydrolase 47 832 VLLPEQSPVSYSK
XRCC1 P18887 DNA repair protein XRCC1 446 TQAAGPSSPQKPP
XRCC1 P18887 DNA repair protein XRCC1 447 QAAGPSSPQKPPT
XRCC1 P18887 DNA repair protein XRCC1 453 SPQKPPTPEETKA
YTHDC1 J3QR07 YTH domain-containing protein 1 308 KRARGISPIVFDR 14-3-3ζ bound phosphoproteins which showed at least five times intensity enhancement from FSK/IBMX treated HeLa cell lysates:
Phospho- Gene names UniProtKB Protein names Sequence window site position
ARHGEF4 E7EV07 Rho guanine nucleotide exchange factor 4 9 ALVRAHSIAGFSP Calcium/calmodulin-dependent protein kinase CAMKK1 Q8N5S9 496 SMLRKRSFGNPFE kinase 1 Calmodulin-regulated spectrin-associated CAMSAP2 Q08AD1 464 GITRSISNEGLTL protein 2 CDK18 Q07002 Cyclin-dependent kinase 18 128 DVSKRLSLPMDIR
DENND4C R4GN35 DENN domain-containing protein 4C 1017 AKHSQPSPEPHSP
DENND4C R4GN35 DENN domain-containing protein 4C 1022 PSPEPHSPTEPPA
DSP P15924 Desmoplakin 2825 MSSAPGSRSGSRS
FAM122B Q7Z309 Protein FAM122B 134 IDFTPVSPAPSPT
FAM122B Q7Z309 Protein FAM122B 138 PVSPAPSPTRGFG
FAM53B Q14153 Protein FAM53B 118 RQCRSLSFSDEMS
FAM83G A6ND36 Protein FAM83G 4 ___MAFSQVQCLD
IRS2 Q9Y4H2 Insulin receptor substrate 2 915 LPPEPKSPGEYIN
IRS2 Q9Y4H2 Insulin receptor substrate 2 1174 NPKRHNSASVENV
KLC1 Q07866 Kinesin light chain 1 631 SRALSASHTDLAH
KLC2 Q9H0B6 Kinesin light chain 2 582 RMKRASSLNFLNK
LMO7 Q8WWI1 LIM domain only protein 7 531 TEIQLPSQSPVEE
LMO7 Q8WWI1 LIM domain only protein 7 533 IQLPSQSPVEEQS Leucine-rich repeat and fibronectin type-III LRFN4 Q6PJG9 585 RPQRSCSLDLGDA domain-containing protein 4 Leucine-rich repeat and fibronectin type-III LRFN4 Q6PJG9 610 WARRSHSVHGGLL domain-containing protein 4 LSR Q86X29 Lipolysis-stimulated lipoprotein receptor 493 RRPRARSVDALDD
MARK2 E9PC69 Serine/threonine-protein kinase MARK2 409 PKQRRFSDQAGPA
MARK3 J3KNR0 MAP/microtubule affinity-regulating kinase 3 442 QKQRRYSDHAGPA
Microtubule-associated serine/threonine- MAST2 Q6P0Q8 74 LLFRKLSNPDIFS protein kinase 2 Microtubule-associated serine/threonine- MAST2 Q6P0Q8 148 NQSLGQSAPSLTA protein kinase 2
MPRIP H0Y2S9 Myosin phosphatase Rho-interacting protein 1772 RNIRSKSLKEGLT
MTSS1L Q765P7 MTSS1-like protein 612 GPTRAGSEECVFY
MYCBP2 O75592 Probable E3 ubiquitin-protein ligase MYCBP2 2873 ERSKSDSYTLDPD
NAV1 Q8NEY1 Neuron navigator 1 142 SKTLSKSEHSLFQ
NAV1 Q8NEY1 Neuron navigator 1 199 VSEDGKSDDELLS
NEDD4L Q96PU5 E3 ubiquitin-protein ligase NEDD4-like 342 SRLRSCSVTDAVA
NF1 P21359 Neurofibromin 876 VSERKGSMISVMS
NOLC1 Q14978 Nucleolar and coiled-body phosphoprotein 1 708 NSIKFDSE_____ Phospho- Gene names UniProtKB Protein names Sequence window site position
OSBPL3 Q9H4L5 Oxysterol-binding protein-related protein 3 303 GPVRLHSSNPNLS
OSBPL3 Q9H4L5 Oxysterol-binding protein-related protein 3 410 ESLLLDSPAVAKS
PARD3 Q8TEW0 Partitioning defective 3 homolog 852 KTRKSKSMDLGIA cGMP-inhibited 3',5'-cyclic PDE3A Q14432 312 KSHRRTSLPCIPR phosphodiesterase A cGMP-inhibited 3',5'-cyclic PDE3A Q14432 520 GALAKISPLSSPC phosphodiesterase A 6-phosphofructo-2-kinase/fructose-2,6- PFKFB2 O60825 466 VRMRRNSFTPLSS bisphosphatase 2 6-phosphofructo-2-kinase/fructose-2,6- PFKFB2 O60825 483 RRPRNYSVGSRPL bisphosphatase 2 6-phosphofructo-2-kinase/fructose-2,6- PFKFB2 O60825 493 RPLKPLSPLRAQD bisphosphatase 2 PPFIBP1 Q86W92 Liprin-beta-1 602 LRRSQSTTFNPDD RAF proto-oncogene serine/threonine-protein RAF1 P04049 315 SPSALSSSPNNLS kinase RAF proto-oncogene serine/threonine-protein RAF1 P04049 316 PSALSSSPNNLSP kinase RBM39 Q14498 RNA-binding protein 39 136 PFRKDKSPVREPI
REEP4 Q9H6H4 Receptor expression-enhancing protein 4 152 GRLRSFSMQDLRS
TNK1 Q13470 Non-receptor tyrosine-protein kinase TNK1 502 MKGISRSLESVLS
TNK1 Q13470 Non-receptor tyrosine-protein kinase TNK1 519 PTGGGSSPPEIRQ
TRIM32 Q13049 E3 ubiquitin-protein ligase TRIM32 335 PEEVVASPRASPA
TRIM32 Q13049 E3 ubiquitin-protein ligase TRIM32 339 VASPRASPAKQRG
YAP1 P46937 Transcriptional coactivator YAP1 105 PEPKSHSRQASTD
YAP1 P46937 Transcriptional coactivator YAP1 109 SHSRQASTDAGTA
YAP1 P46937 Transcriptional coactivator YAP1 110 HSRQASTDAGTAG
YAP1 P46937 Transcriptional coactivator YAP1 163 AQHLRQSSFEIPD
YAP1 P46937 Transcriptional coactivator YAP1 164 QHLRQSSFEIPDD