21.10.2014APC7 antibody (pAb)

Rabbit Anti-Human/Mouse/Rat Anaphase Promoting Complex 7 (ANAPC7)

Instruction Manual

Catalog Number PK-AB718-5731 Synonyms APC7 Antibody: Anaphase promoting complex 7, ANAPC7 Description regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC7 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC7 is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with various transcription coactivators. Quantity 100 µg Source / Host Rabbit Immunogen APC7 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human APC7. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. APC7 antibody can be used for detection of APC7 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunoflourescence starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL. Images Available upon request. References JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol. 2006; 7:644-56. Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. 2001; 262:51-9.

Images Available upon request. Related Products Cat.No. PK-AB718-5731P - APC7 Peptide Cat. No. PK-AB718-1465 - Rat Kidney Tissue Lysate

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGN OSTIC OR THERAPEUTIC PROCEDURES.

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