Product Datasheet

Keratin 20 Antibody

Catalog No: #33418

Package Size: #33418-1 50ul #33418-2 100ul Orders: [email protected] Support: [email protected]

Description

Product Name 20 Antibody

Host Species Rabbit

Clonality Polyclonal

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific

immunogen.

Applications WB IHC

Species Reactivity Hu Ms Rt

Specificity The antibody detects endogenous levels of total Keratin 20 .

Immunogen Type Peptide

Immunogen Description Synthesized peptide derived from human Keratin 20.

Target Name Keratin 20

Other Names Keratin type I cytoskeletal 20; -20; CK-20; K20;

Accession No. Swiss-Prot: P35900NCBI ID: 54474

SDS-PAGE MW 49kd

Concentration 1.0mg/ml

Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide

and 50% glycerol.

Storage Store at -20°C

Application Details

Western blotting: 1:500~1:3000

Immunohistochemistry: 1:50~1:100

Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 20 antibody #33418.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Western blot analysis of extracts from HeLa cells, using Keratin 20 antibody #33418.

Western blot analysis of extracts from HepG2 cells (Lane 2), using Keratin 20 antiobdy #33418. The lane on the left is treated with synthesized peptide.

Background

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine By similarity. Qin Zhou, Mol. Biol. Cell, Jul 2003; 14: 2959. Qin Zhou, J. Biol. Chem., Jun 2006; 281: 16453 - 16461. F Smedts, Am. J. Pathol., Feb 1993; 142: 403. RK Miller, J. Cell Biol., May 1991; 113: 843.

Note: This product is for in vitro research use only and is not intended for use in humans or animals.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2