Product Datasheet
Keratin 20 Antibody
Catalog No: #33418
Package Size: #33418-1 50ul #33418-2 100ul Orders: [email protected] Support: [email protected]
Description
Product Name Keratin 20 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
immunogen.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of total Keratin 20 protein.
Immunogen Type Peptide
Immunogen Description Synthesized peptide derived from human Keratin 20.
Target Name Keratin 20
Other Names Keratin type I cytoskeletal 20; Cytokeratin-20; CK-20; K20;
Accession No. Swiss-Prot: P35900NCBI Gene ID: 54474
SDS-PAGE MW 49kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide
and 50% glycerol.
Storage Store at -20°C
Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Images
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 20 antibody #33418.
Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Western blot analysis of extracts from HeLa cells, using Keratin 20 antibody #33418.
Western blot analysis of extracts from HepG2 cells (Lane 2), using Keratin 20 antiobdy #33418. The lane on the left is treated with synthesized peptide.
Background
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine By similarity. Qin Zhou, Mol. Biol. Cell, Jul 2003; 14: 2959. Qin Zhou, J. Biol. Chem., Jun 2006; 281: 16453 - 16461. F Smedts, Am. J. Pathol., Feb 1993; 142: 403. RK Miller, J. Cell Biol., May 1991; 113: 843.
Note: This product is for in vitro research use only and is not intended for use in humans or animals.
Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2