HBA2 Antibody Cat. No.: 63-935

Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-speci

Western Blot at 1:2000 dilution + human fetal heart lysate Western blot analysis of HBA2 Antibody in mouse liver Lysates/ at 20 ug per lane. tissue lysates (35ug/lane)

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

HOST SPECIES: Rabbit

October 1, 2021 1 https://www.prosci-inc.com/hba2-antibody-63-935.html SPECIES REACTIVITY: Human, Mouse

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Monkey

This HBA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 100-128 amino acids from the Central region of human HBA2.

TESTED APPLICATIONS: Flow, IHC-P, WB

For FACS starting dilution is: 1:25

APPLICATIONS: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

PREDICTED MOLECULAR 15 kDa WEIGHT:

Properties

This antibody is purified through a A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: HBA1

ALTERNATE NAMES: subunit alpha, Alpha-, Hemoglobin alpha chain, HBA1

ACCESSION NO.: P69905

PROTEIN GI NO.: 57013850

GENE ID: 3039, 3040

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

October 1, 2021 2 https://www.prosci-inc.com/hba2-antibody-63-935.html HBA2 located on 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life BACKGROUND: comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF () makes up the remaining 3% of adult hemoglobin. Alpha result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

REFERENCES: 1) Sessa, R., et al. Am. J. Hematol. 85(2):143-144(2010)

2) Sharma, V., et al. Hematology 14(5):297-300(2009)

3) Waye, J.S., et al. Hemoglobin 33(6):519-522(2009)

4) Roy, P., et al. Hemoglobin 33(6):486-491(2009)

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.

October 1, 2021 3 https://www.prosci-inc.com/hba2-antibody-63-935.html