HBA2 Antibody Cat. No.: 63-935
Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-speci
Western Blot at 1:2000 dilution + human fetal heart lysate Western blot analysis of HBA2 Antibody in mouse liver Lysates/proteins at 20 ug per lane. tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Specifications
HOST SPECIES: Rabbit
October 1, 2021 1 https://www.prosci-inc.com/hba2-antibody-63-935.html SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Monkey
This HBA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 100-128 amino acids from the Central region of human HBA2.
TESTED APPLICATIONS: Flow, IHC-P, WB
For FACS starting dilution is: 1:25
APPLICATIONS: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
PREDICTED MOLECULAR 15 kDa WEIGHT:
Properties
This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: HBA1
ALTERNATE NAMES: Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain, HBA1
ACCESSION NO.: P69905
PROTEIN GI NO.: 57013850
GENE ID: 3039, 3040
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
October 1, 2021 2 https://www.prosci-inc.com/hba2-antibody-63-935.html HBA2 located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life BACKGROUND: comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
REFERENCES: 1) Sessa, R., et al. Am. J. Hematol. 85(2):143-144(2010)
2) Sharma, V., et al. Hematology 14(5):297-300(2009)
3) Waye, J.S., et al. Hemoglobin 33(6):519-522(2009)
4) Roy, P., et al. Hemoglobin 33(6):486-491(2009)
ANTIBODIES FOR RESEARCH USE ONLY.
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