Monoclonal Anti-Tis7 Clone IRT537 Purified Mouse Immunoglobulin

Product Number T 2576 Reagent The antibody is supplied as a solution in 0.01 M phos- Product Description phate buffered saline, pH 7.4, containing 15 mM sodium Monoclonal Anti-Tis7 (mouse IgG2a isotype) is derived azide. from the hybridoma IRT537 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Antibody Concentration: ~2 mg/mL Balb/c mice immunized with a synthetic peptide corresponding to amino-acids 30-47 of mouse Tis7, Precautions and Disclaimer conjugated to KLH. The isotype is determined using a Due to the sodium azide content, a material safety data double diffusion immunoassay using Mouse Monoclonal sheet (MSDS) for this product has been sent to the Antibody Isotyping Reagents (Sigma ISO-2). attention of the safety officer of your institution. Consult the MSDS for information regarding hazardous and Monoclonal Anti-Tis7 recognizes human and mouse Tis7 safe handling practices. (~50 kDa). The antibody may be used in ELISA, immunoblotting, and immunocytochemistry. Storage/Stability For continuous use, store at 2-8 °C for up to one month. Tis7 [also known as TPA-Induced Sequence 7 (in mice), For extended storage, freeze in working aliquots. PC4 (in rat) and Interferon-Related Developmental Repeated freezing and thawing is not recommended. Regulator 1 (IFRD1 in humans)] was cloned by differ- Storage in “frost-free” freezers is also not recom- ential screening for cDNAs that are up regulated during mended. If slight turbidity occurs upon prolonged nerve growth factor (NGF) induced neural differentiation storage, clarify the solution by centrifugation before 1 in rat PC12 cells. In adult rats, Tis7 is expressed in use. Working dilution samples should be discarded if skeletal muscle, heart and spleen but not in kidney, liver not used within 12 hours. and brain. In the embryo, the is expressed in the brain, placenta and amnion at 13.5 days of develop- Product Profile ment. Human Tis7 (IFRD1) is 90% identical to By immunoblotting, a working antibody concentration of both rat and mouse , and 48% identical to 0.25-0.5 mg/mL is recommended using extracts of human SKMc15. Tis7 gene is up regulated upon Jun- COS7 cells transfected with mouse Tis7. induced loss of polarity in a mouse mammary epithelial cell line. In the un-polarized cells, the Tis7 protein Note: In order to obtain the best results using various translocates into the nucleus. This protein was found to techniques and preparations, we recommend deter- interact with several proteins of the SIN3 complex, mining optimal working dilutions by titration. including Sin3b, HDAC1, Ncor, and Sap30. This interaction is important for the repression of transcription References 2 of other . Tis7 knockout mice were fertile and 1. Tirone, F., and Shooter, E.M., Proc. Nat. Acad. showed no gross histologic abnormalities. However, Sci. USA, 86, 2088-2092 (1989). there was delayed skeletal muscle regeneration and 2. Vietor, I., et al., EMBO J., 21, 4621-4631 altered skeletal muscle isometric contractile properties (2002). after muscle crush damage and down regulation of 3. Vadivelu, S.K., et al., Molec. Cell. Biol., 24, expression of several muscle-specific genes. Cultured 3514-3525 (2004). primary myogenic satellite cells from Tis7-/- mice KAA/EK 10/05 showed reduced differentiation potential and fusion 3 index.

Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip.