Angela N. Koehler

Broad Institute of MIT and Harvard Office: 617-714-7364 Chemical Biology Program Cell: 617-501-1834 Office 3029 Fax: 617-714-8943 7 Cambridge Center Email: [email protected] Cambridge, MA 02142 Website: www.broadinstitute.org/node/2445

Education

Ph.D. Chemistry, Harvard University, 2003 B.A. Biochemistry and Molecular Biology, Reed College, 1997

Professional Appointments

Assistant Professor, Department of Biological Engineering, Massachusetts Institute of Technology 2014-present Intramural Faculty, David. H. Koch Institute for Integrative Cancer Research at MIT

Investigator, Chemical Biology Program, Broad Institute of MIT and Harvard 2009-2013 Director, Transcriptional Chemical Biology, Broad Chemical Biology Program 2013 Project & Center Manager, Broad NCI Cancer Target Discovery and Development (CTD2) Center 2010-2012

Institute Fellow, Chemical Biology Program, Broad Institute 2003-2009 Director, Ligand Discovery, NCI Initiative for Chemical Genetics (ICG) at Harvard 2003-2009

Research Experience

Graduate Student, Department of Chemistry and Chemical Biology, Harvard University 1998-2003 Laboratory of Professor Stuart L. Schreiber Thesis: Small molecule microarrays: A high-throughput tool for discovering protein-small molecule interactions

Researcher, Department of Chemistry, California Institute of Technology 1997-1998 Laboratory of Professor Barbara Imperiali Project: Biochemical reconstitution of the oligosaccharyltransferase (OST) complex

Undergraduate Researcher, Department of Chemistry, Reed College 1995-1997 Laboratory of Professor Arthur Glasfeld Co-mentored by Professor Richard G. Brennan at Oregon Health Sciences University Thesis: Biochemical and structural characterization of the tRNA-modifying enzyme QueA from Escherichia coli

Teaching Experience

Research Advisor to 32 trainees, including postdoctoral fellows, visiting students, undergraduates, etc. 2003-present Broad Institute, MIT, Harvard University

Instructor, Biochemical Sciences Research for Undergraduates (BS91r) 2004-2006, 2010-2011 Harvard University

Associated Faculty, Experimental Research in the Life Sciences (LS100r) 2009 Harvard University

Associated Faculty, Experimental Molecular and Cellular Biology (MCB100) 2004-2006 Harvard University

Teaching Fellow, Chemical Biology (Chem 170) 1999, 2000 Harvard University, Professor David R. Liu

Teaching Fellow, The Organic Chemistry of Life (Chem 27) 1999 Harvard University, Professor Stuart L. Schreiber

Teaching Assistant, Techniques of Experimental Chemistry 1997-1998 California Institute of Technology, Dr. Jane Raymond

Teaching Assistant, General Chemistry and Biochemical Methods Laboratory Sections 1995-1997 Reed College, Professors Ronald W. McClard and Arthur Glasfeld

Professional Service

Standing Member, NIH Drug Discovery and Molecular Pharmacology (DMP) Study Section 2013-2019 Lawrence H. Summers Fellowship Selection Committee 2012 Ad Hoc Member, NIH Drug Discovery and Molecular Pharmacology (DMP) Study Section 2012 Dana Farber/HCC CURE Program 2011, 2013 Ad Hoc Member, NIH Synthetic and Biological Chemistry B (SBCB) Study Section 2011 Principal Advisor, STEMid 2011-2013 Science of Therapeutics (SofT) Organizing Committee, (Chair starting 2013), Broad Institute 2011-present Chemical Biology Program Leadership Committee, Broad Institute 2010-present Advisory Panel, NRC Canada, Genomics and Health Initiative, BioChips Project 2008-2011 Broad Institute Educational Outreach Program Admissions Panel and Research Mentor 2007-2012 Intel National Science Talent Search Mentor (Megan Blewett, 7th-place out of 1,700) 2006-2007 High-Throughput Screening Review Board, Broad Institute 2005-2012 Chembank Steering Committee, NCI Initiative for Chemical Genetics 2005-2006 Technology Platform Committee, Broad Institute 2004-2006

Honors and Awards

Genome Technology Young Investigator 2012-2013 Kavli Frontiers of Science Scholar, US National Academy of Sciences 2011 Indo-US Frontiers of Science Exchange Award 2011 Eli Lilly Predoctoral Fellowship in Organic Chemistry, Harvard University 2001-2002 MIT 50K Entrepreneur Team Competition, Finalist 2001 Excellence in Scholarship Award, Reed College 1995-1997 William and Jane Einzig Memorial Scholarship, Reed College 1993-1997 ESCO Undergraduate Scholarship, Reed College 1993-1997

Publications

1. Marius Pop, Nicolas Stransky, Colin W. Garvie, Jean-Philippe Theurillat, Timothy A. Lewis, Elizabeth K. Culyba, Fallon Lin, Doug S. Daniels, Raymond Pagliarini, Lucienne Ronco, Angela N. Koehler, Levi. A. Garraway. “A small molecule that binds and inhibits the ETV1 transcription factor oncoprotein.” Submitted, July 2013. 2. Amir Masoud Sadaghiani, Justin Odegaard, Dennis Leveson-Gower, Sang Min Lee, Paul Novick, Olivia M. McPherson, Angela N. Koehler, Robert Negrin, Ricardo E. Dolmetsch, Chan Young Park. “Identification of Orai1 channel inhibitors using minimal functional domains to screen small molecule microarrays.” Submitted, July 2013. 3. Yikai Wang, Jean-Yves Wach, Patrick Sheehan, Cheng Zhong, Chenyang Zhan, Richard Harris, Steven C. Almo, Joshua Bishop, Stephen J. Haggarty, Alexander Ramek, Kayla Berry, Conor O’Herin, Angela N. Koehler, Alvin W. Hung, Damian W. Young. “Fragment-based approach using diversity-oriented synthesis yields a GSK3β inhibitor.” Submitted, July 2013. 4. Izhack Cherny, Maria Korolev, Angela N. Koehler, Michael H. Hecht. “Proteins from an unevolved library of de novo designed sequences bind a range of small molecules.” ACS Synth. Biol., 1, 130-138, 2012. 5. Melissa M. Kemp, Michel Weïwer, Angela N. Koehler.* “Unbiased binding assays for discovering small- molecule probes and drugs.” Bioorg. Med. Chem., 20, 1979-1989, 2012. 6. Dominick E. Casalena, Dina Wassaf, Angela N. Koehler.* “Ligand discovery using small-molecule microarrays.” Chapter, Methods Mol. Biol., 803, 249-263, 2012. 7. Melissa M. Kemp, Qiu Wang, Jason H. Fuller, Nathan West, Nicole Martinez, Elizabeth M. Morse, Michel Weïwer, Stuart L. Schreiber, James E. Bradner, Angela N. Koehler,* “A novel HDAC inhibitor with a hydroxy- pyrimidine scaffold.” Bioorg. Med. Chem. Lett., 21, 4164-4169, 2011. 8. Paul A. Clemons, J. Anthony Wilson, Vlado Dancik, Sandrine Muller, Hyman A. Carrinski, Bridget K. Wagner, Angela N. Koehler, Stuart L. Schreiber. “Quantifying structural property distributions and patterns of performance among sets of small molecules comprising small-molecule screening collections.” Proc. Natl. Acad. Sci. USA, 108, 6817-6822, 2011. 9. Myung-Soo Kang, Eun Kyung Lee, Vishal Soni, Timothy A. Lewis, Angela N. Koehler, Viswanathan Srinivasana, Elliott Kieff. ‘Roscovitine inhibits EBNA1 Serine 393 phosphorylation, nuclear localization, transcription, and episome maintenance.” J. Virol. 85, 2859-2868, 2011. 10. Jermont M. Chen, Anne H. Armstrong, Angela N. Koehler, Michael H. Hecht. “Small-molecule microarrays enable the discovery of compounds that bind the Alzheimer’s Aβ peptide and reduce its cytotoxicity.” J. Am. Chem. Soc., 132, 17015-17022, 2010. This article was featured in the Research Highlights section of Nature Chemistry, November 26 2010. 11. Paul A. Clemons, Nicole E. Bodycombe, Hyman A. Carrinski, J. Anthony Wilson, Alykhan F. Shamji. Bridget K. Wagner, Angela N. Koehler, Stuart L. Schreiber. “Small molecules of different synthetic and natural origins have distinct distributions of structural complexity that correlate with protein-binding profiles.” Proc. Natl. Acad. Sci. USA, 107, 18787-18792, 2010. 12. The Cancer Target Discovery and Development Network: Stuart L. Schreiber, Alykhan F. Shamji, Paul A. Clemons, Cindy Hon, Angela N. Koehler, Benito Munoz, Michelle Palmer, Andrew M. Stern, Bridget K. Wagner, Scott Powers, Scott W. Lowe, Xuecui Guo, Alex Krasnitz, Eric T. Sawey, Raffaella Sordella, Lincoln Stein, Lloyd C. Trotman, Andrea Califano, Riccardo Dalla-Favera, Adolfo Ferrando, Antonio Iavarone, Laura Pasqualucci, José Silva, Brent R. Stockwell, William C. Hahn, Lynda Chin, Ronald A. DePinho, Jesse S. Boehm, Shuba Gopal, Alan Huang, David E. Root, Barbara A. Weir, Daniela S. Gerhard, Jean Claude Zenklusen, Michael G. Roth, Michael A. White, John D. Minna, John B. MacMillan, Bruce A. Posner. “Towards patient-based cancer therapeutics.” Nat. Biotechnol. 28, 904-906, 2010. 13. Angela N. Koehler.* “A complex task? Direct modulation of transcription factors with small molecules.” Curr. Opin. Chem. Biol. 14, Curr. Opin. Chem. Biol., 14, 331-340, 2010. 14. Arturo J. Vegas, Angela N. Koehler.* “Detecting protein-small molecule interactions using fluorous small- molecule microarrays.” Methods Mol. Biol., 669, 43-55, 2010. 15. Carlos Tassa, Jay L. Duffner, Timothy A. Lewis, Ralph Weissleder, Stuart L. Schreiber, Angela N. Koehler, Stanley Y. Shaw. “Binding affinity and kinetic analysis of targeted small molecule-modified nanoparticles.” Bioconjugate Chemistry, 21, 14-19, 2010. 16. Benjamin Z. Stanton, Lee F. Peng, Nicole Maloof, Kazuo Nakai, Xiang Wang, Jay L. Duffner, Kennedy M. Taveras, Joel M. Hyman, Sam W. Lee, Angela N. Koehler, James K. Chen, Julia L. Fox, Anna Mandinova, Stuart L. Schreiber. “A small molecule that binds Hedgehog and blocks signaling in human cells.” Nature Chemical Biology, 5, 154-156, 2009. This article was featured in the Science & Technology Concentrates section of Chemical and Engineering News, 87, 4, 31, 2009. 17. Yingwei Mao, Xuecai Ge, Christopher L. Frank, Jon Madison, Angela N. Koehler, Mary K. Doud, Carlos Tassa, Erin M. Berry, Tracey L. Petryshen, Takahiro Soda, Travis Biechele, Randall T. Moon, Stephen J. Haggarty, Li- Huei Tsai. “DISC1 regulates neural progenitor proliferation via modulation of GSK3β/β-catenin signaling.” Cell, 136, 1017-1031, 2009. Cover image. 18. Shao-En Ong, Monica Schenone, Adam A. Margolin, Xiaoyu Li, Kathy Do, Mary K. Doud, Denkanikota R. Mani, Letian Kuai, Xiang Wang, John L. Wood, Nicola J. Tolliday, Angela N. Koehler, Lisa A. Marcaurelle, Todd R. Golub, Robert J. Gould, Stuart L. Schreiber, Steve A. Carr. “Identifying the proteins to which small-molecule probes and drugs bind in cells.” Proc. Natl. Acad. Sci. USA, 106, 4617-4622, 2009. 19. Angela N. Koehler.* “Microarrays in chemical biology.” Chapter, Wiley Encyclopedia of Chemical Biology, Tadhg Begley, Editor, ISBN: 978-0-471-75477-0, 2009. 20. Arturo J. Vegas, Jason H. Fuller, Angela N. Koehler.* “Small-molecule microarrays as tools in ligand discovery.” Chem. Soc. Rev., 37, 1385-1394, 2008. 21. Thomas J. F. Nieland, Jared T. Shaw, Firoz A. Jaipuri, Jay. L. Duffner, Angela N. Koehler, Sotirios Banakos, Vassilis I. Zannis, Tom Kirchhausen, Monty Krieger. “Identification of the molecular target for small molecule inhibitors of HDL receptor SR-BI activity.” Biochemistry, 47, 460-472, 2008. 22. Katja Schmitz, Stephen J. Haggarty, Olivia M. McPherson, Jon Clardy, Angela N. Koehler.* “Detecting binding interactions using microarrays of natural product extracts.” J. Am. Chem. Soc., 129, 11346-11347, 2007. 23. Arturo J. Vegas, James E. Bradner, Weiping Tang, Olivia M. McPherson, Edward F. Greenberg, Angela N. Koehler, Stuart L. Schreiber. “Fluorous-based small-molecule microarrays for the discovery of histone deacetylase inhibitors.” Angew. Chem. Int. Ed. 46, 7960-7964, 2007. This article was featured in the Science & Technology Concentrates section of Chemical and Engineering News, 85, 40, 30, 2007. 24. Thomas J. F. Nieland, Jared T. Shaw, Firoz A. Jaipuri, Zoltan Maliga, Jay L. Duffner, Angela N. Koehler, Monty Krieger. “Influence of clinical and experimental HDL-cholesterol elevating drugs on the activity of the HDL receptor SR-BI.” J. Lipid Res., 48, 1832-1845, 2007. 25. Hua Miao, John A. Tallarico, Hiroyuki Hayakawa, Karl Münger, Jay L. Duffner, Angela N. Koehler, Stuart L. Schreiber, Timothy A. Lewis. “Ring-opening and ring-closing reactions of a shikimic acid-derived substrate leading to diverse small molecules.” J. Comb. Chem. 9, 245-253, 2007. 26. Jay L. Duffner, Paul A. Clemons, Angela N. Koehler.* “A pipeline for ligand discovery using small-molecule microarrays.” Curr. Opin. Chem. Biol. 11, 74-82, 2007. 27. James E. Bradner, Olivia M. McPherson, Angela N. Koehler.* “A method for the covalent capture and screening of diverse small molecules in a microarray format.” Nature Protocols, 1, 2344-2352, 2006. 28. Nicola Tolliday, Paul A. Clemons, Paul Ferraiolo, Angela N. Koehler, Timothy A. Lewis, Xiaohua Li, Stuart L. Schreiber, Daniela S. Gerhard, Scott Eliasof. “Small molecules, big players: The National Cancer Institute’s Initiative for Chemical Genetics.” Cancer Res. 66, 1-8, 2006. Cover image. 29. James E. Bradner, Olivia M. McPherson, Ralph Mazitschek, David Barnes-Seeman, John P. Shen, Jasmeet Dhaliwal, Jay L. Duffner, Kristen E. Stevenson, Seung Bum Park, Donna S. Neuberg, Paul Nghiem, Stuart L. Schreiber, Angela N. Koehler.* “A robust small-molecule microarray platform for screening cell lysates.” Chem. Biol. 13, 493-504, 2006. This article was featured in the Research Highlights section of Nature Biotechnology, 24, 799, 2006.

Graduate and Undergraduate Studies

30. Angela N. Koehler, Alykhan F. Shamji, Stuart L. Schreiber. “Discovery of an inhibitor of a transcription factor using small molecule microarrays and diversity-oriented synthesis.” J. Am. Chem. Soc. 125, 8420-8421, 2003. 31. David Barnes-Seeman, Seung Bum Park, Angela N. Koehler, Stuart L. Schreiber. “Expanding the functional group compatibility of small-molecule microarrays: discovery of novel calmodulin ligands.” Angew. Chem. Int. Ed. 42, 2376-2379, 2003. Cover image. 32. Paul A. Clemons, Angela N. Koehler, Bridget K. Wagner, Timothy G. Sprigings, David R. Spring, Randall W. King, Stuart L. Schreiber, Michael A. Foley. “A one bead, one stock solution approach to chemical genetics: Part 2.” Chem. Biol. 8, 1183-1195, 2001. 33. Gavin MacBeath, Angela N. Koehler, Stuart L. Schreiber. “Printing small molecules onto microarrays and detecting protein-ligand interactions en masse.” J. Am. Chem. Soc. 121, 7697-7698, 1999. 34. Arthur Glasfeld, Angela N. Koehler, Maria A. Schumacher, Richard G. Brennan. “The role of lysine 55 in determining the specificity of the purine repressor for its operators through minor groove interactions.” J. Mol. Biol. 291, 347-361, 1999.

Patent Applications

1. “Methods for modulating NF-κB.” A. N. Koehler. Harvard University. (61/110,728). 2. “Selective histone deacetylase inhibitors.” A. N. Koehler, J. E. Bradner, J. H. Fuller, N. West. Harvard University. (61/105,589). 3. “Inhibition of Aurora A kinase with spirooxindoles for treatment of cancer, inflammatory, and autoimmune diseases.” K. Münger, A. N. Koehler, H. Hayakawa, C. S. Neumann, M. M. C. Lo, T. A. Lewis, S. L. Schreiber. P.M. Howley. Harvard University. (60/938,362). 4. “Shikimic acid-derived compounds for inhibition and detection of Aurora A-associated tumors.” K. Münger, H. Hayakawa, P. M. Howley, A. N. Koehler, T. A. Lewis, H. Miao, S. L. Schreiber, J. A. Tallarico. Harvard University. (60/841,035) 5. Therapeutic methods using WRN binding molecules.” B. A. Gilchrest, Mark S. Eller, A. N. Koehler, O. M. McPherson, C. S. Neumann, T. A. Lewis. Boston University. (60/823,876). Exclusively licensed. 6. “Small molecule printing.” D. Barnes-Seeman, J. E. Bradner, R. Mazitschek, S. L. Schreiber, A. N. Koehler. Harvard University. (60/755,946). Exclusively licensed. 7. "1,3-Dioxane small molecules that bind with high affinity to human papillomavirus type 16 E2 protein." P. Meneses, A. N. Koehler, J. C. Wong, S. L. Schreiber, P. M. Howley. Harvard Medical School. US 2005/0123902. 8. "Printing small molecule microarrays and detecting protein-ligand interactions en masse." S. L. Schreiber, G. MacBeath, A. N. Koehler, P. J. Hergenrother, K. M. Depew. Harvard University. US Patent 6,824,987. Nonexclusively licensed to three companies.

Invited Seminars

1. Gordon Conference Research Seminar: Bioorganic Chemistry, Proctor Academy, Andover, NH. 6/9/13 2. Yale Chemical Biology Symposium, New Haven, CT. 5/10/13 3. AACR National Meeting, New Paradigms in Molecular Pharmacology Session, Washington, DC, 4/6/13 4. National Cancer Institute, Molecular Discovery Program Seminar Series, Frederick, MD. 4/4/13 5. Massachusetts Institute of Technology, Department of Biological Engineering, Cambridge, MA. 3/21/13 6. Institute for Neurodegenerative Diseases, University of California, San Francisco, CA. 2/28/13 7. RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA. 12/17/12 8. Harvard University, Chemical Biology Practitioner Series, Cambridge, MA. 10/23/12 9. AACR Special Conference on Chemical Systems Biology: Assembling and Interrogating Computational Models of the Cancer Cell by Chemical Perturbations, Boston, MA. 6/30/12 10. Cold Spring Harbor Laboratory, Transcription and Cancer Banbury Meeting, Cold Spring Harbor, NY. 4/10/12 11. Baylor College of Medicine, Department of Pharmacology Seminar, Houston, TX. 2/27/12 12. Harvard Medical School Chemical Biology Boot Camp, Cambridge, MA. 1/19/12 13. Harvard University, Chemical Biology Practitioner Series, Cambridge, MA. 10/13/11 14. National Academy of Sciences, Indo-American Kavli Frontiers of Science Symposium, Irvine, CA. 4/21/11 15. GlaxoSmithKline, Adventures in Chemical Biology Seminar Series, Waltham, MA. 12/3/10 16. Georgetown University Medical Center, Lombardi Comprehensive Cancer Center, Tumor Biology Visiting Professorship Seminar, Washington, DC. 10/22/10 17. Gordon Research Conference Seminar: High-Throughput Chemistry & Chemical Biology, Les Diablerets, Switzerland. 6/22/10 18. St. Lawrence University, Merck/AAAS Seminar in Chemical Biology, Canton, NY. 04/22/10 19. University of Colorado at Boulder, Organic Chemistry Division Seminar, Boulder, CO. 11/16/09 20. Steacie Institute for Molecular Sciences, Institute Seminar, National Research Council of Canada, Ottawa, ON. 10/15/09 21. Midsummer Nights’ Science at the Broad Institute Series, Cambridge, MA. 7/22/09 22. The Museum of Science, Inspiring Minds: Meet Women in Science Program, Boston, MA. 4/30/09 23. Huntsman Cancer Institute, University of Utah, Stem Cell Symposium on Nuclear Control of Cell Growth and Differentiation, Salt Lake City, UT. 2/23/09 24. Stanford University, Joint Seminar for the Departments of Chemistry and Pathology, Stanford, CA. 2/17/09 25. Princeton University, Department of Chemistry Seminar, Princeton, NJ. 12/2/08 26. MIT, Chemistry-Biology Interface Training Program, Cambridge, MA. 11/24/08 27. Harvard Business School, 9th Annual HBS Health Industry Alumni Seminar. Boston, MA. 11/8/08 28. Schering-Plough, Lead Discovery Group Seminar, Kenilworth, NJ. 4/1/08 29. The Museum of Science, The 13th Annual Symposium on Biotechnology Education, Boston, MA. 3/31/08 30. Massachusetts General Hospital, Center for Cancer Research Seminar, Charlestown, MA. 10/22/07 31. National Research Council of Canada, Genomics and Health Initiative Seminar, Halifax, NS. 5/24/07 32. Harvard University, The 4th Annual National Symposium on the Advancement of Women in Science, Panel Discussion on Drug Discovery, Cambridge, MA. 4/14/07 33. The Salk Institute, Salk/Nature/Ipsen Foundation Symposium on Biological Complexity: Diseases of Transcription, Short Talk, La Jolla, CA. 1/13/07 34. University of Wisconsin at Madison, Genome Center Seminar, Madison, WI. 10/19/06 35. University of Michigan, Life Sciences Institute, Chemical Genomics Colloquium, Ann Arbor, MI. 9/15/06 36. Memorial Sloan-Kettering Cancer Center, Molecular Pharmacology Seminar, New York, NY. 2/20/06 37. University of California, Los Angeles, Department of Chemistry and Biochemistry Seminar, Los Angeles, CA. 1/30/06 38. University of California, Berkeley, Organic Chemistry Seminar, Berkeley, CA. 1/27/06 39. Yale University, Organic Chemistry Seminar, New Haven, CT. 1/9/06 40. Massachusetts General Hospital, Cutaneous Biology Seminar, Charlestown, MA. 3/31/03 41. National Cancer Institute, ICMIC/SAIRP Cancer Imaging Meeting, Herndon, VA. 7/24/02 42. Bauer Center for Genomics, Harvard University, Genomics Research Seminar, Cambridge, MA. 4/17/02

Active Federal Funding

1R01 CA160860-01A1 (Koehler, A.N.) 07/01/12-04/30/16 NIH, NCI Developing Direct Small-Molecule Probes of Myc-Dependent Transcription The transcription factor c-Myc is involved in regulating expression of 15% of all genes including several that control cell cycle, growth, proliferation, and differentiation. Deregulation of c-Myc occurs through several mechanisms and is one of the most common oncogenic events in human malignancies. c-Myc, like many oncogenic transcription factors, is a promising yet untested target for cancer therapy due to the lack of potent small molecules that directly modulate Myc function in cells. The overall aim of this project is to develop direct small-molecule probes of c-Myc that will be used to study Myc-dependent transcription in normal and neoplastic cells with the goal of clarifying the potential of c-Myc as a therapeutic target. Role: PI

2R01 HD032067-14A2 (Matzuk, M.M.) 08/17/94-06/30/15 NIH, NICHD Bone Morphogenic Protein Signaling Pathways in Uterine Biology The transforming growth factor 2 (TGF2) superfamily is the largest family of secreted proteins in mammals, and TGF2 signaling pathways have been implicated in a diversity of developmental and pathophysiological processes including preeclampsia. The objectives of this project are to mechanistically define the unique and redundant roles of the bone morphogenetic protein (BMP) type 1 and type 2 receptors during implantation and post-implantation and to identify new drugs for inhibiting or mimicking the functions of BMPs, activins, and myostatin for treatments of clinically important reproductive and non-reproductive diseases. Role: Project Director, PI of subaward from Baylor College of Medicine