PRODUCT INSERT

PREMIXED ANTIBODIES TO THE HUMAN CD3, CD79a, AND MYELOPEROXIDASE ANTIGENS

Product Code Form Volume Tests

CD3-79a-MPO R-PE / PE-Cy™5.5 / FITC 0.75 ml 50 min.

PRODUCT DESCRIPTION Leukocyte Workshop Status:

Premixed antibodies to human CD3, CD79a, and Myeloperoxidase (MPO) for UCHT1: Leukocyte Typing I, III, & IV three-color flow cytometry: HM47: Leukocyte Typing V 8E6: N/A Antibody Clone Isotype Mouse anti-human CD3 R-PE UCHT1 Mouse IgG1 PRODUCT QUALITY CONTROL Mouse anti-human CD79a PE-Cy5.5 HM47 Mouse IgG1 Mouse anti-human MPO FITC 8E6 Mouse IgG1 Each lot is tested by flow cytometry using human peripheral blood leukocytes (PBL). This testing was performed using 15 µl 6 Lot No.: See label Expiration: See label of antibody per 1 x 10 cells in a 100 µl staining volume. Because conditions may vary, it is suggested that each investigator Buffer: Phosphate buffered saline (PBS) determine the optimal amount of antibody to be used for each application. See reverse for representative flow cytometry data. Preservative: 0.1% sodium azide. Sodium azide is an extremely toxic and dangerous compound particularly when combined with acids or metals. REFERENCES: Solutions containing sodium azide should be disposed of properly. 1. Knapp, W., B. Dörken, W. R. Gilks, E. P. Rieber, R. E. Stabilizer: A highly purified grade of BSA has been added as a stabilizing Schmidt, H. Stein, and A. E. G. K. Von dem Borne eds. 1989. agent to bring the final concentration to 4-5 mg/ml after conjugation. Leukocyte Typing IV. Oxford University Press Inc., New York. STORAGE & HANDLING 2. Schlossman, S. F., L. Boumsell, W. Gilks, J. M. Harlan, T.

o Kishimoto, C. Morimoto, J. Ritz, S. Shaw, R. Silverstein, T. Store reagents at 2-8 C. Light exposure should be avoided. Use dim Springer, T. F. Tedder, and R. F. Todd eds. 1995. Leukocyte during handling, incubation with cells and prior to analysis. It is recommended Typing V. Oxford University Press Inc., New York. that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be 3. Mason D. Y., J. L. Cordell, A. G. D. Tse, J. Van Dongen, C. diluted. J. M. Van Noesel, K. Micklem, K. A. F. Pulford, F. Valensi, W. M. Comans-Bitter, J. Borst and K. C. Gatter. 1991. The PRODUCT CHARACTERIZATION IgM-associated protein mb-1 as a marker of normal and neoplastic B Cells. J. Immunol. 147: 2474. Antigen Specificity: 4. Van Noesel, C. J. M., R. A. W. Van Lier, J. L. Cordell, A. G. D. Tse, G. M. W. Van Schijndel, E. F. R. De Vries, D. Y. CD3: This antibody recognizes the ε chain of the CD3/ Mason and J. Borst. 1991. The membrane IgM-associated signaling complex1. This antigen is expressed on T cells, 10-20% heterodimer on human B cells is a newly defined of and a small subset of peripheral blood antigen that contains the protein product of the mb-1 gene. J. with activity. Immunol. 146: 3881.

5. Lanza F., A. Latorraca, S. Moretti, B. Castagnari, L. Ferrari, CD79a: This antibody recognizes the cytoplasmic domain of the CD79a and G. Castoldi. 1997. Cytometry 30: 134. antigen, also known as MB12-4. This protein is expressed early in B cell development and can be found in B cells throughout their 6. Groeneveld, K., J. G. te Marvelde, M. W. M. van den Beemd, development as well as in plasma cells. H. Hooijkaas, and J. J. M. van Dongen. 1996. 10: 1383.

MPO: This antibody detects myeloperoxidase (MPO) in monocytes and granulocytes.

ANALYTE SPECIFIC REAGENT. ANALYTICAL AND PERFORMANCE CHARACTERISTICS ARE NOT ESTABLISHED.

www.invitrogen.com Invitrogen Corporation • 1600 Faraday Avenue • Carlsbad • CA 92008 • Tel: 800.955.6288 • E-mail: [email protected] P/N L11539 Rev. 12/06 DCC-06-0177

PRODUCT INSERT

PREMIXED ANTIBODIES TO THE HUMAN CD3, CD79a, AND MYELOPEROXIDASE ANTIGENS

Lymphocytes:

4 4 4 10 10 10 3 3 3 10 10 10 2 2 2 10 10 10 CD3 R-PE 1 1 1 10 10 10 CD79a PE-Cy5.5 CD79a PE-Cy5.5 0 0 0

10 0 1 2 3 4 10 0 1 2 3 4 10 0 1 2 3 4 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10

MPO FITC CD3 R-PE MPO FITC

Granulocytes:

4 4 4 10 10 10

3 3 3 10 10 10

2 2 2 10 10 10 CD3 R-PE 1 1 1 10 10 10 CD79a PE-Cy5.5 CD79a PE-Cy5.5 0 0 0

10 0 1 2 3 4 10 0 1 2 3 4 10 0 1 2 3 4 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10

MPO FITC CD3 R-PE MPO FITC

The above data were generated using a FACSCalibur™ flow cytometer analyzed with CellQuest™ software, BD Biosciences, San Jose, CA. Quadrant markers were set based on the fluorescence profiles of unstained cells.

Note: Flow cytometric data shown may not necessarily have been generated using the enclosed lot of reagent. For this reason, and due to differences in flow cytometers and cytometer settings, results may vary from those illustrated above. It is suggested that investigators titrate reagents to determine optimal conditions for use in their systems.

ANALYTE SPECIFIC REAGENT. ANALYTICAL AND PERFORMANCE CHARACTERISTICS ARE NOT ESTABLISHED.

www.invitrogen.com Invitrogen Corporation • 1600 Faraday Avenue • Carlsbad • CA 92008 • Tel: 800.955.6288 • E-mail: [email protected] P/N L11539 Rev. 12/06 DCC-06-0177