Catalogue # Aliquot Size
S347-30G-20 20 µg S347-30G-50 50 µg
SETMAR Protein Full length recombinant protein expressed in Sf9 cells
Catalog # S347-30G
Lot # N343 -3
Product Description Purity
Recombinant full-length human SETMAR was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC011635 .
The purity of SETMAR was Gene Aliases determined to be >85% by densitometry. METNASE Approx. MW 66-69 kDa .
Formulation
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Storage and Stability
Store product at –70 oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background
SETMAR or SET domain and mariner transposase fusion gene is a nonhomologous end-joining repair protein that regulates genomic integration of exogenous DNA by opening chromatin and facilitating joining of DNA ends
(1). SETMAR has histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. SETMAR also has DNA nicking activity and may play a role in DNA SETMAR Protein repair (2). Human Pso4 forms a stable complex with Full length recombinant protein expressed in Sf9 cells SETMAR that regulates Metnase function in DNA repair. SETMAR has sequence-specific DNA-binding activity and Catalog Number S347-30G recognizes the 19-mer core of the 5'-terminal inverted Specific Lot Number N343-3 repeats (TIRs) of the Hsmar1 element. Purity >85% Concentration 0.2 µ g/ µl Stability 1yr At –70 oC from date of shipment References Storage & Shipping Store product at –70 oC. For optimal storage, aliquot target into smaller 1. Lee, S.-H.et.al: The SET domain protein Metnase mediates quantities after centrifugation and foreign DNA integration and links integration to store at recommended temperature. nonhomologous end-joining repair. Proc. Nat. Acad. Sci. For most favorable performance, 102: 18075-18080, 2005. avoid repeated handling and multiple freeze/thaw cycles. Product shipped 2. Liu, D.et.al: The human SETMAR protein preserves most of the on dry ice. activities of the ancestral Hsmar1 transposase. Molec. Cell. Biol. 27: 1125-1132, 2007.
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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.