Icofab2020 International Conference on Food, Agriculture and Biotechnology

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Preface The Faculty of Technology, Mahasarakham University (MSU), Thailand initiated the 1st International Postgraduate Symposium on Food, Agriculture and Biotechnology in 2014 (IPSFAB 2014) with the main aims to provide a stage for Thai and international postgraduates to present their research at the international stage. In 2018, the symposium had been renamed as ‘International Conference on Food, Agriculture and Biotechnology (ICoFAB)’ to include postgraduates, researchers and lecturers as primary participants and this has continued so till this day. Our conferences in the past six years (2014 – 2019) have been successful and continued to grow in terms of turn outs and partnerships with international institutes. We have been honored by distinguished scientific committees who kindly have contributed to our Proceedings continually, audiences and renowed keynote/invited speakers from the fields of Food, Agriculture and Biotechnology from around the world. Every year, the potential academic networks or research collaborations amongst Thai and international researchers have been developed. Due to the unprecedented coronavirus pandemic around the globe in 2020, our conference has been transformed to the virtual online format to encourage continuous sharing of research knowledge and close academic networking in spite of social physical distancing. We hope that the 7th ICoFAB2020 virtual conference would bring you fruitful discussions and future research collaborative networks amongst national and international researchers while you can stay safe at home or at workplace during this difficult time.

(Assoc. Prof. Dr. Anuchita Moongngarm) Dean of the Faculty of Technology Mahasarakham University

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Organizing Committee 1. Assoc. Prof. Dr. Anuchita Moongngarm 2. Asst. Prof. Dr. Sirirat Deeseenthum 3. Asst. Prof. Dr. Khanitta Pengmeesri 4. Asst. Prof. Dr. Manatchaya Sungsriin 5. Asst. Prof. Dr. Chakrapong Chaikong 6. Asst. Prof. Dr. Pariyaporn Itsaranuwat 7. Asst. Prof. Dr. Duljira sukboonyasatit 8. Asst. Prof. Dr. Waranyoo Kaewduangta 9. Asst. Prof. Dr. Eakapol Wangkahart 10. Mr. Pongthep Charoensak

MSU Editorial Board for Proceeding 1. Asst. Prof. Dr. Sirirat Deeseenthum 2. Asst. Prof. Dr. Kedsirin Sakwiwatkul 3. Asst. Prof. Dr. Luchai Butkhup 4. Asst. Prof. Dr. Eakapol Wangkahart 5. Asst.Prof. Dr. Ruchuon Wanna 6. Dr. Wanida Chuenta 7. Asst. Prof. Dr. Wasan Duangkhamchan 8. Asst. Prof. Dr. Pheeraya Chottanom 9. Asst. Prof. Dr. Panarat Phadee 10. Assoc. Prof. Dr. Maratree Plainsirichai 11. Assoc. Prof. Prasit Chutichudech 12. Assoc. Prof. Dr. Anut Chantiratikul Associate Editor of Journal of sustainability science and management 1. Assoc. Prof. Dr. Anuchita Moongngarm 2. Asst. Prof. Dr. Vijitra Luang-In 3. Asst. Prof. Dr. Ruchuon Wanna 4. Asst. Prof. Dr. Eakapol Wangkahart 5. Assoc. Prof. Dr. Anut Chantiratikul Editor of Science Technology and Engineering Journal Assoc. Prof. Dr. Sirithon Siriamornpun

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Scientific Committee 1. Prof. Yongqi Shao, Zhejiang University, China 2. Prof. He Yueqiu, Yunnan Agricultural University, China 3. Prof. He Chaoxing, Institute of Vegetable and Flowers. Chines Academy of Agricultural Sciences, China 4. Prof. Ping Zhang, Xishuangbanna Tropical Botanical Garden, China 5. Prof. Jun Zou, College of Fisheries and Life Science, Shanghai Ocean University, China 6. Assoc. Prof. Dr. Bei Wang, College of Fishery, Guangdong Ocean University, China 7. Dr. Song Xiaoming, Zhejiang Academy of Medical Science, China 8. Prof. Dr. Cao Liting, Zhejiang Academy of Medical Science, China 9. Prof. Dr. Li Yutao, Zhejiang Academy of Medical Science, China 10. Chen Jian, Zhejiang Academy of Medical Science, China 11. Asst. Prof. Dr. R. Ranjith Kumar, Kongunadu Arts and Science College, India 12. Assoc. Prof. Dr. V. Selvi, Kongunadu Arts and Science College, India 13. Asst. Prof. Dr. Narendhirakannan R.T., Kongunadu Arts and Science College, India 14. Dr. Indira A. Jayraaj, Kongunadu Arts and Science College, India 15. Dr. K. Kalaivani, Kongunadu Arts and Science College, India 16. Dr. K. Surekha, Kongunadu Arts and Science College, India 17. Asst. Prof. Dr. K. Vel Murugan, Kongunadu Arts and Science College, India 18. Dr. Parasuraman Aiya Subramani, Centre for fish immunology, Vels institute for science, technology and advanced studies, India 19. Asst. Prof. Dr. V. Meiyalagan, Kongunadu Arts and Science College, India 20. Prof. Hanny Wijaya, IPB, Indonesia 21. Assoc. Prof. Suprayogi, Jederal Soedieman University, Indonesia 22. Dr. Condro Wibowo, Jederal Soedieman University, Indonesia 23. Prof. Satoru Kondo, Chiba University, Japan 24. Asst. Prof. Dr. Abdulhadi Albaser, University of Sebha, Libya 25. Assoc. Prof. Dr. Khamsah Suryati Mohd, Universiti Sultan Zainal Abidin, Terengganu, Malaysia 26. Assoc. Prof. Dr. Nyuk Ling Ma, Universiti Malaysia Terengganu, Malaysia 27. Dr. Nurul Huda Abd Kadir, Universiti Malaysia Terengganu, Malaysia 28. Asst. Prof. Dr. Sinouvassane Djearamane, Universiti Tunku Abdul Rahman, Malaysia 29. Dr. Mohd Naqiuddin Husri, Malaysian Palm Oil Board, Malaysia 30. Dr. Nor Omaima Harun, Faculty of Science and Marine Environment, University of Malaysia teregganu, Malaysia 31. Dr. Steve Bird, School of Science, Faculty of Science and Engineering, University of Waikato, New Zealand 32. Dr. Miguel Bao, Department of Seafood Safety and Health, Contaminants and Biohazards, Institute of Marine Research, Norway 33. Dr. Ong Yek Cheng, National University of Singapore, Singapore 34. Assoc. Prof. Dr. Ko Tung Chang, National Pingtung University of Science and Technology, Taiwan 35. Asst. Prof. Dr. Benjawan Chutichudet, Mahasarakham University, Thailand

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

36. Asst. Prof. Dr. Waranyoo Kaewduangta, Mahasarakham University, Thailand 37. Asst.Prof.Dr. Pongsak Khunrae, Microbiology Department, King Mongkut's University of Technology Thonburi, Thailand 38. Assoc. Prof. Dr. Pawinee Chaiprasert, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Thailand 39. Asst.Prof.Dr. Thanaset Senawong, Faculty of Technology, Khon Kaen University, Thailand 40. Asst. Prof. Dr. Vichai Leelavatcharamas, Faculty of Technology, Khon Kaen University, Thailand 41. Asst. Prof. Dr. Rujikan Nasanit, Biotechnology Department, Silpakorn University, Thailand 42. Dr. Supaporn Chunchom, Rajamangala University of Technology Isan, Thailand, Thailand 43. Asst.Prof.Dr. Noppakun Pakdeenarong, Faculty of Science, Mahasarakham University, Thailand 44. Asst. Prof. Dr. Saranyu Khammuang, Faculty of Science, Mahasarakham University, Thailand 45. Asst. Prof. Dr. Rakrudee Sarnthima, Faculty of Science, Mahasarakham University, Thailand 46. Assoc. Prof. Dr. Ratchaneewan Aunpad, Faculty of Allied Health Science, Thammasat University, Thailand 47. Dr. Adisak Romsang, Faculty of Science, Mahidol University, Thailand 48. Asst. Prof. Dr. Surachai Rattanasuk, Science and Technology Department, Roi Et Rajabhat University, Thailand 49. Asst. Prof. Dr. Rossaporn Jiamjariyatam, Faculty of Science, Srinakharinwirot University, Thailand 50. Asst. Prof. Dr. Duangjai Pisuttharachai, King Mongkut’s Institute of Technology Ladkrabang, Prince of Chumphon Campus, Thailand 51. Dr. Supachai Suttijalean, Faculty of Technology, Mahasarakham University, Thailand 52. Mr. Pattana Pasorn, Walairukhavej Botanical Research Institute Mahasarakham University, Thailand 53. Dr. Ahmed Attaya, Department of Biological sciences/University of Massachusetts, Lowell, Massachusetts, USA 54. Dr. Pipat Piewngam, National Institutes of Health, USA

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Program of ICoFAB2020

DAY 1: Wed 29th JULY 2020 MC: Asst. Prof. Dr. Vijitra Luang-In Venue: Webex webinar, live from B412 room, ComCenter, Mahasarakham University, Thailand Time Activities 08.45 – 09.00 Registration

09.00 – 09.15 Report remark by Dean, Faculty of Technology, MSU Welcome speech by MSU President

09.15 – 9.55 Keynote talk 1 ‘Improvement of Grain Protein Content of Rice through Breeding Program’ by Prof. Dr. Totok Agung Dwi Haryanto, Jenderal Soedirman University, Indonesia

9.55 – 10.35 Keynote talk 2 on ‘Tropical plants for functional foods’ by Prof. Dr. Ping Zhang, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, China 10.35 – 10.45 Break

10.45 – 11.10 Invited talk 1 on ‘DNA Aptamers: An emerging tool for biotechnology and drug discovery’ by Assoc. Prof. Dr. Ktittawee Choowongkomon, Kasetsart University, Thailand

11.10 – 11.55 Oral presentations session 1 Chair: Asst. Prof. Dr. Vijitra Luang-In

11.10 – 11.25 1_OBT001 Preparation and characterization of water hyacinth cellulose/keratose composite films Patcharida Chaosri

11.25 – 11.40 2_OBT004 Effects of various process parameters on single cell oil production by Rhodotorula mucilaginosa BJMK43 in molasses cultivation medium Kusumawadee Thancharoen

11.40 – 11.55 3_OAT001 Factors affecting the implementation of GAP among banana (Gros Michel) growers in Ban Lat district, Phetchaburi province, Thailand Almerice Enold

11.55 – 13.00 Lunch

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

13.00 – 13.25 Invited talk 2 on ‘Microalgae as a potential cell factory for biomanufacturing of value added metabolites’ by Assoc. Prof. Dr. Jibu Thomas, Karunya Institute of Technology and Sciences, India 13.25 – 13.50 Invited talk 3 on ‘Water quality monitoring using photosynthetic microbes’ by Prof. Wong Ling Shing, Faculty of Health and Life Sciences, INTI International University & Colleges, Malaysia

13.50 – 14.15 Invited talk 4 on ‘Beneficial feed additives, organic selenium and a nutritional emulsifier, for commercial finfish aquaculture’ by Mr. Brecht Bruneel, ORFFA, Netherlands

14.15 – 14.25 Break

14.45 – 16.00 Oral presentations session 2 Chair: Asst. Prof. Dr. Ekapol Wangkahart

14.45 – 15.00 4_OAT003 Holding capability of different coating material on xylanase and phytase in shrimp feed Nisachon Uniyom

15.00 – 15.15 5_OAT004 In vitro digestibility of fishmeal reduction diet in combination with protease enzyme by Nile tilapia (Oreochromis niloticus) digestive enzyme Pornpot Putnuan

15.15 – 15.30 6_OAT005 Effect of trace minerals supplementation in low fish meal diets on growth performance and immune response of white shrimp (Litopenaeus vannamei) Kanin Patrachotpakinkul

15.30 – 15.45 7_OAT006 Effect of background color and nitrite stress on cooked shrimp color of pacific white shrimp (Litopenaeus vannamei) Kritch Poomkaew

15.45 – 16.00 8_OAT008 Effects of mash feed, sinking and floating feed on growth performance, feed utilization and hematology of Nile tilapia (Oreochromis niloticus) Kamruzzaman Muhammad 16.00 – 17.30 Poster presentation session Poster will be displayed on the screen and can be seen at https://techno2.msu.ac.th/icofab/

END OF DAY 1

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

DAY 2: Thu 30th JULY 2020

MC: Dr. Sunisa Roidoung Venue: Webex webinar, live from B412 room, ComCenter, Mahasarakham University, Thailand

Time Activities 08.45 – 09.00 Registration

09.00 – 09.40 Keynote talk 3 on ‘Meat and meat products as alternative functional foods of animal origins’ by Prof. Dr. Sanchai Jaturasitha, Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Thailand

09.40 – 10.05 Invited talk 5 on ‘Logistics and AI to drive Thai agricultural productivity’ by Asst. Prof. Dr. Kriengkri Kaewtrakulpong, Department of Farm Mechanics, Kasetsart University, Thailand

10.05 – 10.30 Invited talk 6 on ‘Thailand Research Direction’ by Dr. Wiparat De-ong, Deputy Secretary-General, National Research Council of Thailand: NRCT 10.30 – 10.40 Break

10.40 – 11.55 Oral presentations session 3 Chair: Dr. Sunisa Roidoung

10.40 – 10.55 9_OAT007 The effected of temperature humidity index (THI) on egg production in Pradu-Hangdum Chaing Mai high egg production strain chickens Kamonnate Pimrueng 10.55 – 11.10 10_OAT002 Effect of seed quality stimulation in Khao Dawk Mali 105 paddy during the dormancy period by using infrared radiation Chanat Vipattanaporn

11.10 – 11.25 11_OBT003 Probiotic bacteria isolated from Thai fermented foods alleviated anxiety and enhanced anti-stress activities in rats Vijitra Luang-In

11.25 – 11.40 12_OFT001 Development of Kombucha and its functional property from agricultural waste (fermented tea broth) Sawarin Wispen

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

11.40 – 11.55 13_OFT002 The effect of drying with controlling relative humidity of drying air on the color and texture of mango sheet products Kwanchanok Prachunchonakorn

11.55 – 13.00 Lunch

13.00 – 13.25 Invited talk 7 on ‘Microalgae as nutraceuticals and nutritional supplements’ by Asst. Prof. Dr. Sinouvassane Djearamane, Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Malaysia

13.25 – 13.50 Invited talk 8 on ‘Food security – Before, during and post crisis scenario in Malaysia’ By Asst. Prof. Dr. Anto Cordelia T.A.D., Universiti Tunku Abdul Rahman, Malaysia

13.50 – 14.50 Oral presentations session 4 Chair: Dr. Sunisa Roidoung

13.50 – 14.05 14_OFT003 Melatonin, its precursors, total phenolic content and antioxidant activity in different types of legumes germinated under salinity condition Supap Nontasan

14.05 – 14.20 15_OFT004 Drying of ‘Phulae’ pineapple by impingement drying Nattapol Poomsa-ad

14.20 – 14.35 16_OFT005 The management of food boxes packing problems in rectangular container using heuristic method Penpark Sirimark

14.35 – 14.50 17_OFT006 Evaluation of phytochemical constituents and antioxidant activities of different formula from heart herbal teas Rachanee Nammatra

14.50 – 15.00 Closing remarks

THE END

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Contents

Abstracts: Page

Keynote talks: Keynote Talk 1 Improvement of Grain Protein Content of Rice through 3 Breeding Program Prof. Dr. Totok Agung Dwi Haryanto Keynote Talk 2 Development of Functional Foods from Tropical Plants 4 Prof. Dr. Ping Zhang Keynote Talk 3 Meat and meat products as functional foods 5 Prof. Dr. Sanchai Jaturasitha

Invited talks: Invited talk 1 DNA Aptamers: An emerging tool for biotechnology 9 and drug discovery Dr. Ktittawee Choowongkomon Invited talk 2 Microalgae as a potential cell factory for 10 biomanufacturing of value added metabolites Dr. Jibu Thomas Invited talk 3 The Detection of Heavy Metals in Water using 11 Photosynthetic Microbes Prof. Wong Ling Shing Invited talk 4 Beneficial feed additives, organic selenium and a 12 nutritional emulsifier, for commercial finfish aquaculture Mr. Brecht Bruneel Invited talk 5 Logistics and AI to drive Thai agricultural productivity 13 Dr. Kriengkri Kaewtrakulpong Invited talk 6 Direction of Future Research and Innovation in 14 Thailand Dr. Wiparat De-ong Invited talk 7 Microalgae as nutraceuticals and nutritional 15 supplements Dr. Sinouvassane Djearamane Invited talk 8 Food security – Before, during and post crisis scenario 16 in Malaysia Asst. Prof. Dr. Anto Cordelia T.A.D.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Contents

Abstracts: Page

Oral presentation: OBT001 Preparation and Characterization of Water Hyacinth 19 Cellulose/Keratose Composite Films OBT003 Probiotic Bacteria Isolated from Thai Fermented Foods Alleviated 20 Anxiety and Enhanced Anti-Stress Activities in Rats

OBT004 Effects of Various Process Parameters on Single Cell Oil Production 21 by Rhodotorula Mucilaginosa Bjmk43 in Molasses Cultivation Medium

OFT001 Development of Kombucha and Its Functional Property from 22 Agricultural Waste (Fermented Tea Broth)

OFT002 The Effect of Drying with Controlling Relative Humidity of Drying 23 Air on The Color and Texture of Mango Sheet Products

OFT003 Melatonin, Its Precursors, Total Phenolic Content and Antioxidant 24 Activity in Different Types of Legumes Germinated under Salinity Condition

OFT004 Drying of ‘Phulae’ Pineapple by Impingement Drying 25

OFT005 The Management of Food Boxes Packing Problems in Rectangular 26 Container Using Heuristic Method

OFT006 Evaluation of Phytochemical Constituents and Antioxidant 27 Activities of Different Formula from Heart Herbal Teas

OAT001 Factors Affecting the Implementation of GAP among Banana (Gros 28 Michel) Growers in Ban Lat District, Phetchaburi Province, Thailand

OAT002 Effect of Seed Quality Stimulation in Khao Dawk Mali 105 Paddy 29 during the Dormancy Period by Using Infrared Radiation

OAT003 Holding Capability of Different Coating Material on Xylanase and 30 Phytase in Shrimp Feed

OAT004 In Vitro Digestibility of Fishmeal Reduction Diet in Combination 31 with Protease Enzyme by Nile Tilapia (Oreochromis niloticus) Digestive Enzyme

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Contents

Abstracts: Page OAT005 Effect of Trace Minerals Supplementation in Low Fish Meal Diets 32 on Growth Performance and Immune Response of White Shrimp (Litopenaeus vannamei)

OAT006 Effect of Background Color and Nitrite Stress on Cooked Shrimp 33 Color of Pacific White Shrimp (Litopenaeus vannamei)

OAT007 The Effect of Temperature Humidity Index (THI) on Egg 34 Production in Pradu-Hangdum Chaing Mai High Egg Production Strain Chickens

OAT008 Effects of Mash Feed, Sinking Feed and Floating Feed on Growth 35 Performance, Feed Utilization and Hematology of Nile Tilapia (Oreochromis niloticus)

Poster Presentations: PBT001 Rice Straw Hydrolysate as a Promising Culture Medium for 39 Astaxanthin Production by the Red Yeast Xanthophyllomyces dendrorhous

PBT002 Diversity of Halotolerant and Halophilic Bacteria in Na Si Nuan 40 Secondary Forest Soil at Maha Sarakham, Thailand

PBT003 Enhancement of Cordycepin, Adenosine in the Cultivation of 41 Cordyceps militaris by Elicitation

PBT004 Improvement of Ethanol Production from Yam Bean by 42 Thermotolerant Yeast Saccharomyces Cerevisiae Rmu Y-10 Using an Orthogonal Array Design

PBT005 Phytochemicals and Antioxidation of Fractionated 43 Sugarcane Extracts: Suphanburi 50 Variety

PBT006 Neuroblastoma Cell-Line Toxicity due to Increased 44 Alzheimer’s-Related Proteins and Cell Death Subsequent to High-Dose Palmitic Acid Exposure

PBT007 In Vitro Cytotoxicity of Cordyceps militaris Extracts on Different 45 Human Cancer Cell Lines

PFT001 Quality Characteristics of Reduced-Fat Vienna Sausage Using Rice 46 Flour and Skim Milk Powder Mixture as a Fat Replacer

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Contents

Abstracts: Page PFT002 Pro-Inflammation Cytokine Secretion of Peripheral Blood 47 Mononuclear Cells by Edible Mushroom

PFT003 Method Validation for Quantitative Determination of Gallic Acid 48 from Acacia concinna (Willd.) D.C.

PFT004 Effect of Gelation Addition on Physico-Chemical Characteristics 49 of Bastard Oleaster Gummy Jelly

PFT006 Development of Ready-To-Drink Powder from Carrot, Orange and 50 Lemon Using Foam Mat Drying

PFT007 Efficacy of Gamma Irradiation on Sprout Inhibition, Insect 51 Infestation and Antioxidant Activity of Fresh Black Galingale (Kaempferia parviflora) for Prolonged Storage

PFT008 Investigation the Physical, Mechanical Properties of Edible Film 52 from Riceberry flour

PFT009 Effect of Storage and Packaging Conditions on Physical Properties 53 and Carotenoid Content of Mango Tablet

PFT010 Impact of Lactic Acid and Hot Water Treatment Combined on 54 Quality Change of Storage Shucked Hard Clam (Meretrix casta)

PFT011 Optimization of Pulsed Vacuum Osmotic Dehydration of Model 55 Food and Application for Important Compounds Enrichment

PFT012 Production of High Melatonin and Antioxidant Activity Mungbean 56 Sprout by Germination of Mungbean Seeds under Salinity and Exogenous Melatonin

PFT013 Unripe Banana Flour Improved Resistant Starch Content and 57 Quality Characteristics of Gluten-Free Rice Cookies

PFT014 Physicochemical Properties of Dried Guizhou as Affeced by 58 Pretreatments, Drying Techniuques and Storage Period

PFT015 Formulation Optimization of Purple Rice-Coconut Milk Drink 59 Based on Physicochemical Properties using Mixture Design

PAT001 Development of Rice Quality to Good Agricultural Practice 60 Standards of Farmers in Mukdahan Province

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Contents

Abstracts: Page PAT002 Potential of Bitter Bush Against Maize Weevil 61 (Sitophilus zeamais)

PAT003 The Nutritive Value and Bioactive Compounds of Alfalfa 62 (Medicago sativa) Grown at Burapha University, Sa Kaeo Campus

PAT004 Effect of Pectic Oligosaccharides from Fruit Peels as Prebiotic in 63 Animal Feed

PAT005 Toxicity and Bioactivity of Powder and Essential Oil of Climbing 64 Wedelia Against Maize Weevil

PAT006 Effects of Alternate Wetting and Drying Techniques on Grain Yield 65 and Water Use Efficiency in Irrigated Rice

PAT007 Sprout Contain Higher Phenolic, Flavonoids, and Antioxidant 66 Activity than Seed in Different Thai Varieties of Mungbeans (Vigna radiata L.)

PAT008 The Comparative of Growth and Carcass Performance in Native 67 Chickens (Chee): KKU 12 and Chee N

PAT009 The Effect of Oil and Non Structural Carbohydrate (NSC) Sources 68 in Different Ratios of Roughage to Concentrate in Total Mixed Ration (TMR) Diet on Total Gas Production and Nutrient Digestibility on In Vitro System

PAT010 Effect of Dietary Papaya Peel and Baker’s Yeast Supplementation 69 on Growth Performance, Hematological Value of Silver Barb (Barbonymus gonionotus) and Its Resistance to Aeromonas hydrophila

PAT011 The Effect of the Seasons to the Changes of Water Quality and 70 Sediment Quality in the Coastal Area of the Andaman Sea in Trang Province, Thailand

PAT012 Microplastic Contamination Assessment in Freshwater Bivalves: A 71 Case Study of Ubolratana Reservoir

PAT013 Dietary Fishmeal Replacement with Crickets and Bamboo 72 Caterpillar Meal for Cobia (Rachycentron canadum)

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Contents

Abstracts: Page PAT014 Immune Response Analysis of Fairy Shrimp Branchinella 73 thailandensis Against Bacterial Black Disease by de novo Transcriptome Analysis

PAT015 Using the K-Nn Weather Generator Algorithms in Predicting 74 Evaporation for Reference Crop Evapotranspiration

PAT016 Growth of Caesalpinia sappan L. by Using Different Growing 75 Media and Evaluation of Antioxidant Activity of Foliage

PAT017 Effects of Probiotics on Survival, Blood Chemical of Nile Tilapia 76 under Transportation Stress

PAT018 A Survey of Cattle Rumen Fluke at Butcher Shops in 77 Maha Sarakham Province, Thailand

PAT019 Utilzation of Melon Husk on Productive Performance, Egg Quality 78 and Bone Quality of Laying Hens

PAT020 Antimicrobial Activity of Some Herbal Plant Extraction and 79 Biological Fermented Products from Nasinuan Sub-District, Maha Sarakham, Thailand

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Keynote talks

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Keynote Talk 1

Improvement of Grain Protein Content of Rice through Breeding Program

Totok Agung Dwi Haryanto*, Agus Riyanto and Dyah Susanti

Laboratory of Plant Breeding and Biotechnology Faculty of Agriculture, Jenderal Soedirman University

Corresponding Author: [email protected]

For the next 100 years, food will remain a global issue besides energy. Therefore, each country continues to make every effort to facilitate food for its country. Especially at this time, the co-19 pandemic afflicts all countries, and encouraging all to improve food production. Indonesia as one of the agrarian countries and as a world food barn committed to improve rice production through the development of superior varieties of rice as a source of food and nutrition. In line with the government’s effort, Jenderal Soedirman University developed rice breeding program to gain superior varieties. Four superior national varieties of rice have been released as national superior varieties, namely Inpago Unsoed 1 (aromatic upland rice), Inpari Unsoed 79 Agritan (high salinity tolerant rice), Inpago Unsoed Parimas (drought tolerant upland rice), and this year has passed the trial of releasing varieties, Inpago Unsoed Protani (drought tolerant upland rice with high protein content). Breeding program to improve the yield and grain protein content of rice in Laboratory of Plant Breeding and Biotechnology, Faculty of Agriculture, Jenderal Soedirman University was conducted from 2007. The objective of the research was to improve grain protein content of rice through breeding program. It started by germplasm characterization to provide database for parentals screening. In 2008, base population of lowland and upland rice varieties with high yield, high grain protein content, and early maturity character was generated from hybridization. Genetic study of inheritance pattern of grain protein content character was conducted on the second generation of Ciherang x Milky Rice crosses to provide information of heritability, gene number and gene actions. Half diallel analysis was used for grain protein content genetic study using Hayman method. Lines selection using pedigree method carried out in F2 to F5 population, followed by selection on high-protein content, high-yielding, and early maturity rice lines. To study yield stability and genotype x environment interaction, multi location test were conducted. Result of these research showed that grain protein content of rice is a character controlled by polygenes located in nucleus without sitoplasmic maternal effect, and has high broadsense heritability. Grain protein content was not correlated with yield components. Genotype x environment interaction influenced grain protein content of rice. Locations with lower monthly rainfall produce higher protein content, depend on genotypes. A genotype already released as a new cultivar of rice with high grain protein content (9.8 %) and high yielding potential (9.06 t/ha) in upland.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Keynote Talk 2

Development of Functional Foods from Tropical Plants

Ping Zhang

Professor, Group leader, Nutritional Immunology and Functional Foods, CAS Key Lab of Tropical Plant Resources and Sustainable Use, Chinese Academy of Sciences, Menglun, Yunnan, P.R.China 666303

Tropical plants represent a huge repertoire for exploring novel nutrients or phytochemicals ideal for the development of functional foods. In the past several years, we have successfully identified important plant resources from xishuangbanna tropical area in southwest China for their extraordinary beneficial effects on health, particularly on metabolic disease. One example is Zanthoxylum myriacanthum var. pubescens Huang, which is known as maqian in xishuangbanna, an aromatic plant used as a spice and also in Dai folk medicine. We showed that the essential oil extracted from the fruits of maqian (MQEO) exhibited very strong anti-diabetic effects in three different mice models. MQEO not only reversed diabetic symptoms rapidly but also restored damaged pancreatic β-cells. By using MIN-6 pancreatic β-cells, we proved that MQEO was highly effective in β-cell protection by suppressing endoplasmic reticulum stress. Another example is bamboo shoot. We isolated insoluble dietary fiber from bamboo shoot Dendrocalamus hamiltonii and investigated its anti-obesity effect in high-fat diet fed mice. Our results showed that this bamboo shoot fiber was the most effective in suppressing high-fat diet induced body weight gain compared with commonly consumed dietary fibers in China. With next generation sequencing we showed that bamboo shoot fiber prevented high-fat diet induced loss of diversity in the gut microbiota and highly promoted the growth of Bacteroidetes. Furthermore, we also found that dietary fiber isolated from bamboo shoot D. latiforus exhibited similar anti- obesity effect. Both bamboo shoot fibers were highly effective in maintaining insulin sensitivity. Our work with two typical tropical plants in xishuangbanna proved that tropical plants have huge potential for the development of functional foods for the prevention or treatment of metabolic diseases and they deserve more attention from academics and food industry.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Keynote Talk 3

Meat and Meat Products as Functional Foods

Sanchai Jaturasitha

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

Meat and meat products are sources of diets contain important component of nutrients with a selective positive effect on one or more functions of the organism. Food from animal origin demonstrate to beneficially effects on the target functions in body beyond proper nutritional effects. Meat is rich in protein and particularly essential amino acids which contains numerous endogenous bioactive compounds. Regarding meat products, are mainly directed transformation by modifying and reformation of added active ingredients. Meat industry are more concerned the potential health- promoting and bioactive characteristics as functional foods.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited talks

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited Talk 1

DNA Aptamers: An Emerging Tool for Biotechnology and Drug Discovery

Kiattawee Choowongkomon

Associate Professor, Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 11000, Thailand

The aptamers are short single-stand DNA or RNA or short peptide that can bind with targets in three-dimensional structure with high affinity and specificity. Aptamers, that can be bound the target molecule with high specificity and affinity, have been generated from small molecules such as ions, drugs, and toxins, to large molecules, such as peptides, proteins, and even whole cells, bacteria, and viruses. Advantages of aptamers are high affinity and specificity, chemical synthesis, long-term storage, and ease of modification. The aptamers also provide an economic and a superior alternative to antibodies. In addition, the aptamers show minimal or no immune response in vivo. Aptamers can be used as a drug against cancer and viruses and as biosensors. Colorimetric method that is a technique for develop aptamer to biosensor because colorimetric use the property of gold nanoparticles for detect the interaction of target and sensor. In our research, we used capillary electrophoresis (CE) technique in SELEX for selected specific aptamers of several targets such as paraquat, organophosphate, HIV-1 reverse transcriptase, and porcine respiratory syndrome virus (PRRSV).

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited Talk 2

Microalgae as a Potential Cell Factory for Biomanufacturing of Value Added Metabolites

Jibu Thomas

Lab Head -Algae Biomass Research Laboratory and Deputy Director, Centre for Food Science and Technology, Department of Biotechnology, School of Agriculture and Biosciences, Karunya Institute of Technology and Sciences (Deemed University), Coimbatore 642114, Tamil Nadu, India

E-mail: [email protected] Mobile: +91 9443017893

Microalgae are present in all existing earth ecosystems, not just aquatic but also terrestrial, representing a big variety of species living in a wide range of environmental conditions. It is estimated that more than 50,000 species exist, but only a limited number have been studied and analyzed. They are prokaryotic or eukaryotic photosynthetic microorganisms with unicellular or simple multicellular structures that can grow rapidly. Their uses are reported to be plenty, ranging from chemicals including vitamins, pigments, antioxidants or oils (omega-3 fatty acids) and proteins as well as animal feed. Much attention was gained on biomass for the production of biofuels from microalgae. Our study was focused on exploring the unexplored area and isolate, identify and characterize the micralgal community using various analytical tools. Primary objective was that native algae provide an ideal platform for additional strain development and optimization. Exploring this biota offers a diverse base of organism naturally adapted to regions that have potential like waste treatment/phycoremediation and bioresource production. Our review will have a focus on ecophysiological diversity of fresh water microalgae isolated from catchment region of river Noyyal of Western ghats India, phycoremediation potential of isolated microalgae species on domestic and industrial wastewaters, biomolecules, intracellular lipids and fatty acids profiles of the isolates.

Keywords: Microlagae; Biomolecules; GC-MS; Fresh water; FTIR; Diversity; Metabolites

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited Talk 3

The Detection of Heavy Metals in Water using Photosynthetic Microbes

Wong Ling Shing

Professor, Faculty of Health and Life Sciences INTI International University Malaysia

Environmental pollution is a serious global issue. Pollutants, such as heavy metals flow into water resources threaten the well-being of living organisms. The issue was worsen by the increasing pace of urbanization, agriculture, and mining activities. Therefore the development of low cost, rapid, and easy to use biological-based tools to assist the current available analytical tools is important, to maintain the quality of our water resources. Photosynthetic cells have been proven a promising bio-element for biosensors, especially for heavy metals detection in environment. The presence of heavy metals changes many metabolic pathways in the cells, including the photosynthetic pathways. These changes can be used to indicate the presence of heavy metals. The biological signals trigger by these changes can be transformed and amplified using electronic transducers. Some of the biosensors are able to detect the presence of heavy metals to part per billion (ppb) level, within a short period of time. The storability and selectivity of these microbe based biosensor however, remain a big challenge in biosensor development.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited Talk 4

Beneficial Feed Additives, Organic Selenium and a Nutritional Emulsifier, for Commercial Finfish Aquaculture

Brecht Bruneel1 and Eakapol Wangkahart2

1ORFFA, The Netherlands, 2 Research Unit of Excellence for Tropical Fisheries and Technology, Division of Fisheries, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Khamriang Sub-District, Kantarawichai, Mahasarakham, Thailand

The fast expansion of aquaculture during the last decades has resulted in excessive demands for aquafeed and key ingredients such as fish meal. Functional feed additives are nutritive/nonnutritive compounds supplemented in the diet of fish and shrimp for specific purposes such as enhancement of the physicochemical properties of feed or performance of target species. There are numerous functional feed additives serving for these purposes, each with a different chemical composition and origin that can be categorized in different groups. Selection of the potential feed additives should be based on their effects, contribution price, and commercial availability. In the present study we selected organic selenium and a nutritional emulsifier, as potential feed additives in fish. These feed additives have shown enhancement effects on growth, immune system, survival rate (after pathogenic challenge), digestive enzyme activities and fatty amino acids contents of aquatic species.

Keywords: Nile tilapia; Lipid level; Nutritional emulsifier; Growth performance; Body composition; Digestive enzyme; Fatty acids

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited Talk 5

Logistics and AI in Agriculture to Drive Thai Agricultural Productivity

Kriengkri Kaewtrakulpong

Department of Farm Mechanics, Kasetsart University, Bangkok, Thailand

Unpredictable climate change, environmental impacts, labor shortages, and adaptation behaviour are the major problems to maintain the optimum agricultural production in Thailand. To solve these crisis, a transboundary approach is required in the field of agricultural machinery using smart agriculture, logistics, and AI to drive the sustainable goals for Thai agriculture. Also, big data obtained from sensor, IoT, and computer vision is utilised via machine learning algorithms and high performance computing in attempt to create new opportunity to unravel, quantify, and understand data intensive process in agricultural operational environment, leading to more accurate and faster decision making, and evolving into intelligent agriculture to increase agricultural production level and bio-product quality. Therefore, the aim of the session is to focus on the recent achievements, challenges and future research directions to achieve food security and safety for Thailand via logistics and AI in agriculture.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited Talk 6

Direction of Future Research and Innovation in Thailand

Wiparat De-ong

Deputy Secretary-General, National Research Council of Thailand: NRCT

Research and Innovation System in Thailand refers to innovative activities undertaken by stakeholders including academic institution or University as a knowledge producer, corporations or community as a knowledge consumer, and governments as a facilitator. As a result of restructuring Thailand Research and Innovation System, Ministry of Higher Education, Science, Research and Innovation (MHESI) is formulated by integrating Science and Technology Ministry, Office of the Higher Education Commission and Office of National Research Council of Thailand (NRCT). Therefore, MHESI, has determine the direction of Thailand research and innovation system, including 4 possible missions as follow:  Smart Worker/Citizen Development by setting human development platform for 3 groups, including firstly, quality bachelor production to respond 21st century jobs market; secondly, future skill development both upskill and reskill for new labourforce; thirdly, education preparation for aging population.  Shifting Thai society to value based economy by promoting demand driven of research and development in order to reduce social and economic disparity or improve quality of life; to increase Thailand competitiveness focusing on 10 S-curve manufacture target, as well as responding to grand tatent (for example, Zero waste, Al for All and so on)  Promoting Thailand to be innovation nation in order to change economic structure from made in Thailand to innovated in Thailand such as setting young startup fund, creating community innovation system, and so on. - Reinventing University in order to increase its role to create frontier research and frontier knowledge and to be R&D center of community. - In order to drive 4 possible missions above, MHESI has innovated new funding management by setting Science, Research and Innovation Promotion Fund and Program Management Unit of Thailand (PMU). According to National Strategy and the Twelfthand National Economic and Social Development plan, NRCT is the main of PMU and innovation organization dealing with and supporting research for developing economy, industry, society, the environment and for driving the country into prosperity, security and sustainability according to the National Strategy.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited Talk 7

Microalgae as Nutraceuticals and Nutritional Supplements

Sinouvassane Djearamane

Assistant Professor, Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Malaysia

While the human population continues to grow, there is a concomitant rise in demand for healthy food and health products. Sources of nutritious biomass capable of fulfilling this demand are rampantly pursued. Thanks to their rapid growth, health benefits, and enriched compounds they produce, algae have shown viability in meeting nutritious demands. Microalgae have been utilized as food and supplement by humans for thousands of years due to their high nutritional properties such as proteins, essential fatty acids, vitamins, minerals, carotenoids and other essential nutrients. Currently, microalgae are being grown and harvested much more intensively as the algae's role in human health and nutrition continues to increase. The use of currently developed algae includes primarily as food, food additives, aquaculture, dyes, cosmetics, pharmaceuticals, and nutraceuticals. For human usage, only a small fraction of the total number of algal organisms is cultivated. There are possibly more species of algae which have not yet been identified and characterized for their nutritional values. Therefore, in the coming years, the potential for algal use in food intake, nutritional supplements, energy production, and much more is likely to intensify. Further, geographical use of algae for human nutrition will offer parallel distribution and unique characteristics and ability of the ecosystem for growth. Recently, increased research and developmental activities are being carried out to increase the production and usage of these algae to satisfy the ever-increasing demand and to recognize the benefits of additional algae types.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Invited Talk 8

Food Security - Before, During and Post Crisis Scenario in Malaysia

Anto Cordelia Tanislaus Antony Dhanapal

Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, Malaysia

United Nation defines food security as the availability of safe, sufficient and nutritious food to maintain an active and healthylife. Malaysia is ranked 28 out of 113 countries in the Global Food Security Index, and is also among the lowest in expenditure for agricultural research and development. The Covid-19 pandemic signalled an alarm evoking worldwide concern about food security because of border closures, closure of food outlets and restaurants and countries imposing ban of exports that affected the food supply chain. As of today, Malaysia is more food secure, meeting various internationally-accepted criteria, unlike in the past, especially during the colonial period. Malaysia is self-sufficient in all major foods to meet market demand, which is not equated with human needs. Production has improved for poultry and vegetables, while rice production is still supplemented by imports. The access for food is no longer an issue for most Malaysians with better amenities for processing, logistics, storage and distribution systems. Yet, the affordability of food has remained a very serious issue, with the weakening ringgit against the US dollar, which inflates the food price.Malaysia has witnessed a nearly five-fold leap in both food and exports and imports in the last decade whereby we not presently at food crisis. Covid-19 pandemic is a wake-up call for countries to prepare for future food crises. Empowering farmers to increase production will simultaeously empower consumers and also improve the supply chain upstream and downstream along the way.All countries and governments including Malaysia should revamp their agricultural sector and must be prepared to ensure food and supply chain continuity despite any future hindrance in the system.

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Oral presentations

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OBT001

Preparation and Characterization of Water Hyacinth Cellulose/Keratose Composite Films

Patcharida Chaosri and Prasong Srihanam*

Department of Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

This research aimed to extract cellulose from water hyacinth for preparation of composite films with different ratios of hair keratose. The keratose film has more transparency than other films. All films were then investigated their morphology, secondary structure, and thermal properties using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectrophotometer, and Thermogravimetric analyzer (TGA), respectively. The results found that the native keratose film has smooth surfaces without phase separation, while the cellulose film has rough and some fibers appeared on its surface. The cellulose/keratose composite films have specific absorption peaks of each polymer functional groups. The secondary structure of the keratose film has a β- pleated sheet resulting in the fragile texture and brittle. Adding cellulose increased flexibility and thermal properties of the keratose film. This was due to the keratose and cellulose formed interaction via functional groups. The result suggested that cellulose and keratose are well compatible together. The finding results might be advantaged to use as basic information to prepare for the cellulose/keratose composite films obtaining desire properties for applications.

Keywords: Film; Cellulose; Keratose; Secondary structure; Thermal property

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OBT003

Probiotic Bacteria Isolated from Thai Fermented Foods Alleviated Anxiety and Enhanced Anti-Stress Activities in Rats

Vijitra Luang-In1,*, Sutisa Nudmamud-Thanoi2 and Arjan Narbad3

1Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand 2Centre of Excellence in Medical Biotechnology, Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand 3Quadram Institute Bioscience, Norwich Research Park, Colney, Norwich NR4 7UA, UK

*Corresponding Author: [email protected]

This research investigated the safety and probiotic attributes of six bacteria maninly isolated from Thai fermented foods and also their effects on anxiety-like behaviors in Wistar rats pretreated with antibiotics 7 days prior to probiotic supplementation for 14 days. These bacteria all possessed safety and probiotic properties based on various assessments. In a rat behaviour model, the results of marble burying test and nestlet shredding test relating to anxiety behaviours showed that PRO group (rats received probiotics for 14 days) had the least anxiety level which was significantly less than those in ANT group (rats without probiotics). Similarly, anti- stress and antioxidant activities results showed that PRO group had the highest anti- stress CAT activity and FRAP antioxidant activity. PRO group showed the highest recognition index which was significantly higher than those in ANT group based on novel recognition test indicating probiotics enhanced short-term memory. Our probiotics cocktail can modulate rat behaviours.

Keywords: Anxiety; Neurotransmitter; Stress; Probiotics; Thai fermented foods

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OBT004

Effects of Various Process Parameters on Single Cell Oil Production by Rhodotorula Mucilaginosa Bjmk43 in Molasses Cultivation Medium

Kusumawadee Thancharoen*, Aussama Karatoom, Wantana Tanwawwaew and Piyatida Budwong

Department of Biology, Faculty of Science and Technology, Rajabhat MahaSarakham University, Nakronsawan Road, Mueng, Maha Sarakham 44000, Thailand

*Corresponding Author: [email protected]

Rhodotorula mucilaginosa BJMK43, an oleaginous yeast isolated from Baby jack fruit (Momordica cochinchinensis, Lour), was grown on molasses under nitrogen- limited media. The capacity this with respect to biomass production and lipid content was evaluated. The influence of molasses and ammonium sulfate concentration were investigated. The results illustrated that molasses as a carbon source at 20% (w/w) and 1.0 g/L ammonium sulfate under incubation temperature of 30 °C, 150 rpm and 144 h for lipid production. Under the optimized condition the yeast gave maximum biomass 12.89±0.33 g/L and lipid content 56.53±3.98%. The dominant fatty acids exhibited in order of their relative abundance (%w/w), were stearic (C18:0), palmitic (C16:0), myristic (C14:0), and pentadecanoic (C15:0) acids. This suggests that this strain could be used as a good feedstock for biodiesel production.

Keywords: Ammonium sulfate; Biodiesel; Fatty acid; Molasses; Oleaginous yeast

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OFT001

Development of Kombucha and Its Functional Property from Agricultural Waste (Fermented Tea Broth)

Sawarin Wispen1, Chalat Santivrangkana1, Pimpinan Somsong2, Pimsiri Tiyayon2 and Warangkana Srichamnong1,*

1Food Science Unit, Institute of Nutrition, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand 2School of Agricultural Resources, Chulalongkorn University, Bangkok, Thailand

*Corresponding Author: [email protected]

The aim of this project was to develop a functional product from fermented tea broth which is a by-product of fermented tea (Miang). The broth that was from the 15th day fermented tea was used. The broth was boiled with the additional of 10% sugar for brix adjustment to make original and pineapple kombucha. Original fermented broth kombucha were produced form mixing with water and fermented broth (1:1.5) and add (1:1) pineapple for pineapple favor. After that, they were mixed with scoby and 3% of previous kombucha which produced from fermentation of Komagataeibacter saccharivorans, Zygosaccharomyces bailii, Dekkera bruxellensis. The analysis of antioxidant activity, total phenolic content and flavonoids content were performed at interval of 0, 1, 3, 5, 7, 9 and 11 day. The result showed that total phenolic of both original and pineapple kombucha were reduced. Total phenolic contents of original kombucha was in ranged of 32.1 - 42.05 µmol GAE/ 100ml. Total phenolic contents of pineapple favour was in ranged of 23.1 ± 0.67 - 29.57 ± 1.19 µmol GAE/ 100ml. While antioxidant (FRAP & ORAC) slightly increased in both batched. Antioxidant activity on FRAP of original kombucha was 311,527.8 ± 38,978.9 µmol TE / 100ml. Antioxidant activity on ORAC of original kombucha and pineapple favor were highest at day 7 (620,442.1 ± 79,695.4 and 506,842 ± 212,017.1 µmol TE / 100ml respectively). Total flavonoids were decreased from 0th day fermented tea kombucha as it formed dimer and complex structure (10.15 – 6.92 µmol CE / 100ml). Therefore, per serving size the developed kombuchas contained high amount of both phenolics and flavonoids. Fermented broth can be developed into Kombucha that containing functional property compounds.

Keywords: Fermented tea; Kombucha; Antioxidant; Value added

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OFT002

The Effect of Drying with Controlling Relative Humidity of Drying Air on The Color and Texture of Mango Sheet Products

Kwanchanok Praphunchonakorn and Weerachet Jittanit*

Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Road, Chatuchak, Bangkok 10900, Thailand

*Corresponding Author: [email protected]

Mango sheet is one of the traditional mango products that is produced for preservation and market-value addition purposes. Currently, the factory encounters the problem about the long drying time requirement of this product. Therefore, this research aimed to increase the efficiency of drying mango sheets by proposing the hot air drying method that controlled the relative humidity (RH) of drying air. In this study, three drying schemes namely conventional hot air drying (60 C), single-stage drying (60 C/15% RH), and two-stage drying (80 C for 1 h 5 min in the 1st stage and then adjusted to 60 C/15% RH in the 2nd stage) were applied in the mango sheet drying until reaching the moisture content of approximately 16.8% wet basis (w.b.) which is comparable to the commercial product. The color and textural properties, consisting of firmness and adhesiveness of mango sheets dried by these methods were determined and compared with those of the commercial product. It appeared that all of the drying schemes required shorter drying time than that of the current practice in the factory, especially when the relative humidity of drying air was controlled. The color intensity of the sample dried by conventional hot air drying product was the lowest, followed by the two-stage, and single-stage drying respectively whereas all the color values of commercial product was greater than its counterparts. In addition, the firmness and adhesiveness of conventionally-dried samples were higher than the two-stage dried sample, commercial product, and single-stage dried mango sheets respectively. This research proved that the drying process that applied the relative humidity control which is the similar concept to the heat pump dryer could noticeably shorten the drying time compared to the general hot air drying methods while its product quality was not much different from the commercial product.

Keywords: Drying; Heat pump; Hot air drying; Mango sheet; Relative humidity control

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OFT003

Melatonin, Its Precursors, Total Phenolic Content and Antioxidant Activity in Different Types of Legumes Germinated under Salinity Condition

Supap Nontasan, Pheeraya Chotthanom and Anuchita Moongngarm*

Research Unit of Nutrition for Life, Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

Legumes are a potent source of bioactive components that could be enhanced by seed germination under salinity condition. The effect of germination under salinity compared with normal conditions was evaluated for levels of melatonin, its precursors, total phenolic compounds and antioxidant activity of selected legume seeds. Five types of legume seeds including soybeans (Glycine max), black beans (Phaseolus vulgaris), red kidney beans (Phaseolus vulgaris), chickpeas (Cicer arietinum) and lentils (Lens culinaris) were germinated in the presence of sodium chloride (67 mM NaCl) and absence of NaCl for 72 h, while ungerminated legume seeds served as the control. Results indicated that legume seeds were a good dietary source of melatonin, serotonin and free tryptophan as well as total phenolic compounds (TPC). Germination of legume seeds in the presence and absence of NaCl stimulated melatonin, serotonin, and tryptophan content, and also exhibited stronger antioxidant activity than ungerminated seeds. Levels of melatonin, serotonin, TPC and antioxidant activity were enhanced in soybeans, black beans, and lentils germinated under salinity condition, while NaCl- treated chickpeas and red kidney beans exhibited higher levels of melatonin, TPC and antioxidant activity than seeds germinated without NaCl. Findings demonstrated germination of legume seeds under suitable salinity stress as a promising alternative technique to improve levels of melatonin, serotonin, free tryptophan content and antioxidant activity in legume sprouts.

Keywords: Melatonin; Serotonin; Tryptophan; Total phenolic compounds; Legumes

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OFT004

Drying of ‘Phulae’ Pineapple by Impingement Drying

Nattapol Poomsa-Ad1,* and Lamul Wiset2

1Drying Technology Research Unit, Faculty of Engineering, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, Thailand 2Postharvest Technology and Agricultural Machinery Research Unit, Faculty of Engineering, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

The research aims to study the effects of temperature and nozzle hole diameter by impingement drying technique on drying kinetic of Phulae pineapple, energy consumption and color of product. The pineapple was sliced at a diameter of 6 cm and 0.5 cm thick. The specific air flow rate was fixed throughout the experiment at 0.555 kg s-1/ kg of product. Temperatures of 45, 60 and 75 °C and nozzle holes diameter of 0.75, 1.50 and 3.00 cm were conditions used in this study. Results found that drying at 75 °C with nozzle hole diameter of 1.50 cm had the highest drying rate and the lowest specific energy consumption. The drying time and specific energy consumption were 500 min and 0.23 kWh/kg of evaporated water. However, when consider in color, it was found that the optimal condition was drying at 60 °C with 1.50 cm nozzle hole diameter.

Keywords: Drying characteristics; Hot air drying; Specific energy consumption

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OFT005

The Management of Food Boxes Packing Problems in Rectangular Container Using Heuristic Method

Sirimark Penpark1,*, Boontan Thawatcha2 and Yotmanee Sancha3

1Department of Science and Mathematics, Rajamangala University of Technology Isan, Surin, Thailand 2Faculy of Science and Technology, Rajabhat Maha Sarakham University, Maha Sarakham, Thailand 3Faculy of Science and Technology, Phuket Rajabhat University, Phuket, Thailand

*Corresponding Author: [email protected]

The increasing of consumer demand in foods resulted in their delivery problems, especially the packing, moving and shipping of food boxes into a limited rectangular container from the food manufacturing or postal business. Therefore, this research purposed to manage the problem for three-dimensional food boxes packing, to reduce the spaces, costs and time in the shipments using the heuristic algorithms base on genetic algorithm (GA) and simulated annealing (SA). The computational performance of both models is then compared. The results provide the reduction of costs in shipments with the use minimum number of containers.

Keywords: Management; Food packing; Heuristic method; Genetic algorithm; Simulated annealing

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OFT006

Evaluation of Phytochemical Constituents and Antioxidant Activities of Different Formula from Heart Herbal Teas

Rachanee Nammatra1,*, Thanida Srihawong2 and Chinnaphat Chaloemram1

1Biodiversity Program, Walai-Rukhavej Botanical Research Institute, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150, Thailand 2Veterinary Technology and Veterinary Nursing Program, Faculty of Agricultural Technology, Rajabhat Mahasarakham University, Mueang Maha Sarakham District, Maha Sarakham 44000, Thailand

*Corresponding Author: [email protected]

Heart tonic herbal teas were first developed to select herbal teas for prevent and enhance cardiovascular health. The herbal teas were separated to 2 formulas: first, Pleurotus ostreatus: Pandanus amaryllifolius: Oryza sativa (brown rice): Oryza sativa (leaves): Clitoria ternatea; 1: 1:1:1:1 (w:w:w:w;w) and second, Ganoderma lucidum: Caesalpinia sappan: Aegle marmelos: Chrysanthemum indicum: Carthamus tinctorius; 1: 1:1:1:1 (w:w:w:w;w) were extracted using by aqueous. Phytochemical screening was determination on flavonoid and phenolic acid compounds using the High-Performance Liquid Chromatography (HPLC), total phenolic content (TPC) and total flavonoid content (TFC) of extract. The α-glucosidase inhibitory assay and antioxidant activities were determined by, 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging and Ferric reducing antioxidant Power (FRAP) assay. The results showed that, the 2nd formula contained significantly higher TPC, TFC, flavonoid compounds and phenolic compounds than the 1st formula. Antioxidant activities with DPPH and FRAP assay of the 2nd formula (16.88 ± 0.25 mgTE/g and 62.97 ± 2.45 mM Fe2+ E/g, respectively) was significantly higher than the 1st formula (5.41 ± 0.03 mgTE/g and 13.57 ± 1.10 mM Fe2+ E/g, respectively). This finding indicated that heart tonic herbal tea formula 1 does have inhibitory free radical effect and has a potential to be consumed as a herbal tea for cardiovascular health.

Keywords: Heart tonic herbal teas; CVDs; Phytochemical screening; Antioxidant

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OAT001

Factors Affecting the Implementation of GAP among Banana (Gros Michel) Growers in Ban Lat District, Phetchaburi Province, Thailand

Almerice Enold, Mana Kanjanamaneesathian and Alisa K. Yoshida*

Faculty of Animal Science and Agricultural Technology, Silpakorn University Phetchaburi IT Campus, Cha-Am, Phetchaburi 76120, Thailand

*Corresponding Author: [email protected]

Banana (Gros Michel) or Kluai Hom Thong (Musa acuminata, AAA Group) is an economic crop in Thailand and it is an important product to export under the standard of good agricultural practice (GAP). This study aims to investigate factors affecting the GAP implementation among banana (Gros Michel) growers who adopt GAP for practice in Ban Lat district, Phetchaburi province. This study revealed that most of farmers encountered water scarcity but they realized the importance of recording the data during the GAP practice. Gender, number of family members and farming organization membership were the factors highly impacting the implementation of GAP among these banana growers. The results from this study should direct the staffs in the relevant organizations to focus on these three key issues to improve growing banana with GAP certification.

Keywords: Gros Michel banana; Good agricultural practice; Ban Lat, Phetchaburi

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OAT002

Effect of Seed Quality Stimulation in Khao Dawk Mali 105 Paddy during the Dormancy Period by Using Infrared Radiation

Chanat Vipattanaporn1,*, Cherdpong Chiawchanwattana2, Juckamas Laohavanich1, Phirayot Khaengkan3 and Suphan Yangyuen1

1Mechanical Engineering, Faculty of Engineering, Mahasarakham University, Maha Sarakham 44150, Thailand 2Farm Mechanics Program, Department of Technical Education in Mechanical Engineering, Faculty of Technical Education, Rajamangala University of Technology Isan Khonkaen campus, Khon Kaen 40000, Thailand 3Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

The aim of the study was to break the dormancy of rough rice of Oryza sativa L., cultivar Khao Dawk Mali 105 (KDML105), by using infrared radiation for seed quality (in this research, including seed vigour and seed germination) stimulation under different factors including storage periods of 3, 5, 7 and 9 weeks; infrared wavelengths of 4.00, 3.75 and 3.52 µm; and duty-cycles of radiation of 1, 2 and 3 cycles. The feed rate was controlled at 20 kg.hr-1 and the seed moisture content did not exceed 14% wb. It was found that the storage period, infrared wavelength and duty-cycle of radiation significantly affect the seed quality at P≤0.05. For good performance, we recommend a storage period of 5 weeks, an infrared wavelength of 3.75 µm and a duty-cycle of 2 cycles.

Keywords: Seed dormancy; Germinated rough rice; GABA; Infrared ray; Seed vigour; Seed germination

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OAT003

Holding Capability of Different Coating Material on Xylanase and Phytase in Shrimp Feed

Uniyom Nisachon1, Chumkam Srinoy2 and Jintastaporn Orapint1,*

1Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Thanon Ngamwongwan, Lat Yao, Chatuchak, Bangkok 10900, Thailand 2Faculty of Agricultural Technology, Valaya Alongkorn Rajabhat University, Pathum Thani, Thailand

*Corresponding author: [email protected], [email protected]

The enzyme leaching from different coating materials on white shrimp (litopenaeus vannamei) diets was conducted by focusing on finding the solution of using enzyme on top in aquaculture feed with coating materials. The study was assigned in completely randomized design (CRD). To compare the ability of individual coating materials, diet coated with enzyme, phytase or xylanase, and without coating materials represented as control and 3 coating materials including chitosan, pullulan and seal4 were observed on phytase and xylanase leaching at 15 and 30 min. The result shows the less leaching of phytase were coated by pullulan with 32.88 and 34.45% leaching after immersion 15 and 30 min, respectively. Furthermore, the coating materials that suitable for xylanase enzyme were also pullulan with 0.075% and 0.078% leaching after immersion 15 and 30 min, respectively.

Keywords: Phytase; Xylanase; Chitosan; Pullulan; Seal4

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OAT004

In Vitro Digestibility of Fishmeal Reduction Diet in Combination with Protease Enzyme by Nile Tilapia (Oreochromis niloticus) Digestive Enzyme

Pornpot Putnuan1, Orapint Jintasataporn1,* and Srinoy Chumkam2

1Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand 2 Faculty of Agricultural Technology, Valaya Alongkorn Rajabhat University under the Royal Patronage, Pathum Thani, Thailand

*Corresponding author: [email protected]

The in vitro digestibility of fishmeal reduction diet in combination with protease enzyme by Nile tilapia (Oreochromis niloticus) digestive enzyme was conducted and assigned in Factorial in Complete Randomized Design (Factorial 2x2 in CRD). Factor A was fishmeal levels of 5%Fishmeal(5%FM) and 0%Fishmeal(0%FM). Factor B was protease enzyme supplementation of no protease enzyme supplementation and with protease enzyme 100 unit/Kg feed supplementation. The results showed that both of the 5%FM and 0%FM inclusion rate and exogenous protease enzyme supplementation without Nile tilapia digestive enzyme crude extract supplementation showed no significantly differences (p>0.05) on protein, carbohydrate and phosphorus digestibility. Incubated mash feed with enzyme from Nile Tilapia liver showed the significantly increasing of protein digestion (p<0.05) in diets of 0%FM both in diet of without and with exogenous protease enzyme supplementation but no significantly differences (p>0.05) on carbohydrate and phosphorus digestibility. Incubated mash feed with enzyme from Nile Tilapia intestine showed no significantly differences (p>0.05) on protein, carbohydrate and phosphorus digestibility. Therefore, digestive enzyme in Nile tilapia itself has high efficacy to digest feed materials than exogenous protease enzyme, however, the enzyme efficacy depended on the quality and concentration of exogenous enzyme.

Keywords: in vitro digestibility; Exogenous protease; Tilapia digestive enzyme; Fishmeal reduction diet

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OAT005

Effect of Trace Minerals Supplementation in Low Fish Meal Diets on Growth Performance and Immune Response of White Shrimp (Litopenaeus vannamei)

Kanin Patrachotpakinkul1,*, Orapint Jintasataporn2 and Srinoy Schumkam3

1,2 Department of Aquaculture, Faculty of Fisheries, Kasetsart University 3Faculty of Agricultural Technology, Valaya Alongkorn Rajabhat University under the Royal Patronage

*Corresponding Author: [email protected], [email protected]

Low fish meal diets is the hot issue in the animal industry including aquafeed since thirty years ago because an insufficient amount of fish meal production, unsustainable fishery, and labors problems. To reduce fish meal in the diets that may lack off some essential minerals especially trace minerals such as zinc, copper, manganese, and selenium then effect to the animal performance. Minerals act as cofactors of a variety of enzymes and improving growth performance, metabolism, and immune system of shrimp especially in case of low fish meal feed because fish meal is the rich source of minerals from nature. Hence, several trace mineral concentration in the Litopenaeus vannamei diets was studied in the topic of growth performance and immune response of white shrimps. The trial was assigned in CRD with 3 treatments and each of 10 replicates. The diets with 37% crude protein and 5% crude fat were formulated and top up with different trace mineral levels of 1x, 2x, and 3x. All diets were fed to shrimp for 4 weeks and the results showed no significant differences (P>0.05) on all growth performance parameters. Anyway, the numerical values of growth performance had an improving trend when increasing mineral concentrations. Furthermore, an immune response such as total haemocyte count, phenoloxidase activity, and glutathione showed no significant difference between treatments (P>0.05) but phenoloxidase activity showed increasing numerical values when mineral concentration had a higher dose. Therefore, the results of this study showed that an increasing dose of trace minerals in the white shrimp diet trend to promote growth performance and could improve the immune response.

Keywords: Trace minerals supplementation; Low fish meal diets; Litopenaeus vannamei; Growth performance; Immune response

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OAT006

Effect of Background Color and Nitrite Stress on Cooked Shrimp Color of Pacific White Shrimp (Litopenaeus vannamei)

Kritch Poomkaew1, Orapin Jintasataporn1,* and Srinoy Chumkam2

1Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand 2Faculty of Agricultural Technology, Valaya Alongkorn Rajabhat University, Pathum Thani, Thailand

*Corresponding Author: [email protected]

The effect of background color and nitrite stress on cooked shrimp color of pacific white shrimp (litopenaeus vannamei) was conducted. Two factors of A: back ground color, black and white and B: no nitrite stress and with nitrite stress condition at 10 ppm were assigned in the complete randomized factorial designed (Factorial 2x2 in CRD) then 4 treatments and 3 replicates were studied. Shrimp with average weight of 7.43-8.21 gr./ind. were exposure in treatment condition for 1 hr. until 24 hr. then sampling for boiled and color measurement by colorimeter and salmofan to compare the red intensity of cooked shrimp color. The results showed that in short term periods of 1 hr. after exposure to new environment of black and white background and nitrite stress condition at 10 ppm, the black background condition could promote the cooked shrimp color both in normal condition and under nitrite stress condition better than white background condition. The nitrite stress condition at 10 ppm could enhance the cooked shrimp color in short term periods of 1 hr. better than normal condition. For long term exposure to nitrite stress condition at 10 ppm for 24 hr., the stress condition reduce the redness of cooked shrimp color. These contradicted with the short term exposure that enhance cooked shrimp color.

Keywords: Cooked shrimp color; Background color; Nitrite stress

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OAT007

The Effect of Temperature Humidity Index (THI) on Egg Production in Pradu-Hangdum Chaing Mai High Egg Production Strain Chickens

Kamonnate Pimrueng1, Doungnapa Promket2,*, Khanitta Pengmeesri2 and Jennarong Kammongkun3

1Graduate student, Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasalakham University, Maha Sakham 44000, Thailand 2Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasalakham University, Mahasakham 44000, Thailand 3Chiang Mai Livestock Research and Breeding Center, San Pa Tong District, Chiang Mai 50120, Thailand

*Corresponding Author: [email protected]

The purpose of this research was to study the effects of climate changes on egg production under Chiang Mai Livestock Research and Breeding Center in Chiang Mai Province. This study was conducted from 300 Pradu-Hangdum Chiang Mai high Egg Production strain chickens. The data record collected from April 2019 until March 2020. The study indicated that, the weather was hot and humid. The temperature ranged from 22.28 - 31.23 °C and humidity range were 46.70 - 81.80%. The highest temperature in May (31.12°C) and April (31.23 °C) the lowest temperature in December (22.28 °C). Daily temperature and humidity data from meteorological measurements in Chiang Mai were recorded daily over the duration of the trial period were used to calculate the temperature humidity index (THI). The THI group for effect on egg production composition was set to 4 groups (THI1 is THI ≤ 70, THI2 is ≤ 74 THI > 70, THI3 is ≤ 78 THI >74 and THI4 is THI > 78). The highest THI values (THI 4) effect on age at first egg (AFE), THI4 (144.59 day) was lower than THI1 (161.40 day), THI2 (158.22 d) and THI3 (156.17 d). AFE in summer season was lower than raining season. The correlation between TEP270 and average egg at 1 mouth (AEM) traits were found the highest correlation (0.90). Moreover, the correlation between weight at first egg (HWFE) and age at first egg (AFE) was 0.38, it meaning that HWFE resulting in AFE faster.

Keywords: Temperature humidity index (THI); Egg production; Pradu-Hangdum Chiang Mai high Egg Production Strain

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

OAT008

Effects of Mash Feed, Sinking Feed and Floating Feed on Growth Performance, Feed Utilization and Hematology of Nile Tilapia (Oreochromis niloticus)

Muhammad Kamruzzaman1,3, Orapin Jintasataporn1,* and Srinoy Chumkam2

1Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand 2Faculty of Agricultural Technology, Valaya Alongkorn Rajabhat University, Pathum Thani, Thailand 3Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh.

*Corresponding Author: [email protected]

The present study was conducted to evaluate the effects of mash feed, sinking feed and floating feed on growth performance, feed utilization and hematology of Nile tilapia (O. niloticus). The study was assigned in CRD with three treatments and four replicates. Fish with average weight 13.30± 0.85 g. (means ± SD) were fed three experimental diets mash feed, sinking feed and floating feeds that had same feed formula. The fish were fed experimental diets at the rate of 3.0-5.0 % of their body weight per day, twice daily for four weeks. At the end of the experiment, there were no significant (P>0.05) differences among the treatments in weight gain (WG), average daily gain (ADG), feed conversion ratio (FCR). Specific Growth rate (SGR) was significantly (P< 0.05) different among the treatments. The high SGR was found in fish fed floating feed, followed by fish fed sinking feed, whereas lower value was found in mash feed. Fish fed floating and sinking feed exhibited a significant (p<0.05) higher level of serum protein. In this case, the level of serum protein was lower in mash feed. However, no significant differences (P>0.05) were observed for red blood cell (RBC) count, hemoglobin (Hb) concentration, hematocrit (Ht) percentage and immunoglobin (IgM) level. Results from this study demonstrated that fish fed floating and sinking feed both improved growth performance better than mash feed.

Keywords: Feed type; Growth performance; Hematology; Nile tilapia

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Poster Presentations

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PBT001

Rice Straw Hydrolysate as a Promising Culture Medium for Astaxanthin Production by the Red Yeast Xanthophyllomyces dendrorhous

Pasinee Phoproek1 and Jidapha Tinoi2,3*

1Interdisciplinary Program of Biotechnology, Graduate School, Chiang Mai University, Chiang Mai, Thailand 2Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Faculty of Science, Chiang Mai University 3Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand

*Corresponding Author: [email protected]

This research aimed to utilize rice straw as a low-cost substrate for astaxanthin production by Xanthophyllomyces dendrorhous TISTR 5730. Rice straw composed of high content of cellulose (35.92±0.51 %) and hemicellulose (21.13±1.93 %). After enzymatic hydrolysis with commercial cellulase, the rice straw hydrolysate contained the maximum of total reducing sugar content of 89.82±0.39 g/L. X. dendrorhous TISTR 5730 was cultivated on rice straw hydrolysate with 20 g/L of initial total reducing sugar as a culture medium. The maximum cell dry weight (5.47±1.45 g/L) with astaxanthin concentration (2.49±0.04 mg/L) was achieved. The kinetics parameters demonstrate the astaxanthin productivity of 0.50±0.01 mg/L/day with the astaxanthin coefficient of 0.13±0.00 mg/g sugar consumed. X. dendrorhous TISTR 5730 was capable to consume the reducing sugar in rice straw hydrolysate up to 97.08±0.03 %. The absorption spectra, HPLC profile and FTIR spectrum of the produced astaxanthin X. dendrorhous TISTR 5730 were characterized and compared to standard astaxanthin. This research indicated that rice straw hydrolysate could be used as a promising culture medium for astaxanthin production.

Keywords: Rice straw; Culture medium; Enzymatic hydrolysis; Astaxanthin production; Xanthophyllomyces dendrorhous yeast

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PBT002

Diversity of Halotolerant and Halophilic Bacteria in Na Si Nuan Secondary Forest Soil at Maha Sarakham, Thailand

Kannika Chookietwattana* and Thalisa Yuwa-Amornpitak

Department of Biotechnology, Faculty of Technology, Mahasarakham University, Thailand

*Corresponding Author: [email protected]

The Na Si Nuan Secondary Forest at Maha Sarakham, Thailand, can be considered as a distinct ecosystem due to a non-saline property among saline soil of the other areas of the province. Soil samples were collected seasonally from 14 plots within the Na Si Nuan Secondary Forest. The halotolerant-, slightly halophilic-, and moderately halophilic-bacteria were isolated from soil samples using the halobacteria medium containing 0, 3, and 6 % w/v sodium chloride, respectively. Fifty-five representative bacterial isolates were selected for characterization using the combination of phenotypic characterization, and 16S rDNA sequence analysis. They belonged to 16 different species within 5 genera namely: Bacillus, Enterobacter, Janibacter, Rhodococcus, and Staphylococcus. The dominant species belonged to the genus Bacillus.

Keywords: Bacterial diversity; Bacterial flora; Soil bacteria; Saline soil; Salt-affected areas

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PBT003

Enhancement of Cordycepin, Adenosine in the Cultivation of Cordyceps militaris by Elicitation

Jitsuda Kullawat1, Pheeraya Chottanom2, and Luchai Butkhup1*

1Natural Antioxidant Innovation Research Unit (NAIRU), Department of Biotechnology, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, Thailand 2Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, Thailand

*Corresponding Author: [email protected]

Cordyceps militaris is a medicinal mushroom that has been widely used in Asia for the treatment of various diseases. The pharmacological activity of this mushroom is primarily attributable to the cordycepin and adenosine. In this study, to obtain the fruit bodies with improved content of active constituents, we examined the effect of 7 elicitor 2+ 2+ 2+ 2+ include CaCl2 (Ca ), CuSO4.5H2O (Cu ), ZnSO4(Zn ), FeSO4.7H2O(Fe ), salicylic acid, gibberellic acid and L-phenylalanine on the biosynthesis of cordycepin and adenosine by added to cultivation medium and using Plackett–Burman design for experimental. Results showed that the cordycepin, adenosin production could be effect 2+ by all elicitor but that the CaCl2 (Ca ), CuSO4.5H2O and L-phenylalanine addition had highest effect to the C. militaris growth and increased cordycepin, adenosine production. The maximum cordycepin content (10,071.40 ± 8.97 mg/kg dw) was observed in run 8, whereas minimum cordycepin content was revealed in run 6 (1,850.04 ± 6.11 mg/kg dw). The information obtained could helpful for the hyperproduction of cordycepin by cultivation of C. militaris on a large scale.

Keywords: Codyceps militaris; Cordycepin; Adenosine; Elicitor; Plackett–Burman design

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PBT004

Improvement of Ethanol Production from Yam Bean by Thermotolerant Yeast Saccharomyces cerevisiae RMU Y-10 Using an Orthogonal Array Design

Kanlayani Charoensopharat*1 and Kitipong Wechgama2

1Department of Biology, Faculty of Science and Technology, Rajabhat Maha Sarakham University, Maha Sarakham 44000, Thailand 2Department of Agricultural Technology and Environment, Faculty of Sciences and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima 30000, Thailand

*Correspondent Author: [email protected]

The objective of the present study was to investigate the optimum condition for ethanol production from Yam bean juice by thermotolerant yeast Saccharomyces 4 cerevisiae RMU Y-10 using L9 (3 ) an Orthogonal array design. Three parameters: yeast extract (YE; 3, 6 and 9 g/L), diammonium hydrogen phosphate (DAP; 0.25, 0.50 and

0.75 g/L) and magnesium sulfate (MgSO4; 0.5, 1.0 and 1.5 g/L) were optimized, all batch fermentations were carried out at 37 C. The results reveal that the order of these parameters influences on ethanol concentration was YE > DAP > MgSO4, respectively. The optimal condition for ethanol production was yeast extract 9 g/L, diammonium hydrogen phosphate 0.75 g/L and magnesium sulphate 0.5 g/L. The highest concentration of ethanol (51.2 g/L) was obtained at 72 h of fermentation time, while the ethanol yield and productivity were 0.51 and 0.71 g/L.h, respectively. The results also showed the ethanol concentration under the optimal condition which was 29.78  higher than those in YM medium (control 1) and Yam bean juice only (control 2).

Keywords: Ethanol production; Yam bean; Thermotolerant yeast; Orthogonal array design

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PBT005

Phytochemicals and Antioxidation of Fractionated Sugarcane Extracts: Suphanburi 50 Variety

Phongsathorn Motham, Ansaya Thonpho and Prasong Srihanam*

Department of Chemistry, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]; [email protected]

The objective of this work is to fractionate the Suphanburi 50 sugarcane extract using silica gel column chromatography and screen its phytochemical contents and antioxidation in each fraction. The phytochemicals including total phenolic, flavonoids, saponin, proanthocyanidin and condensed-tannin were varied by the fractions depending on the eluting solvents. Moreover, the antioxidation of the fractionated extracts were also varied following the eluting solvents. All phytochemicals were positively corelated to all antioxidation methods, but in variable values. The obtained results indicated that the sugarcane, Suphanburi 50 variety is a natural good source of phytochemicals and exposed antioxidant activity which would be supported health benefit.

Keywords: Sugarcane; Suphanburi 50; Fractionation; Phytochemical; Antioxidation

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PBT006

Neuroblastoma Cell-Line Toxicity due to Increased Alzheimer’s-Related Proteins and Cell Death Subsequent to High-Dose Palmitic Acid Exposure

Phansa Phitthayaphong1,2,3, Sirinart Kumfu1,2,3, Nipon Chattipakorn1,2,3 and Siriporn C. Chattipakorn1,3,4*

1Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand 2Cardiac Electrophysiology Unit, Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand 3Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai 50200, Thailand 4Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand

*Corresponding Author: [email protected]; [email protected]

Obesity caused by high-fat diet (HFD) consumption is one of the risk factors for the development of Alzheimer’s disease (AD). Previous studies have reported that saturated fatty acids from an HFD, particularly palmitic acid (PA), promotes Alzheimer’s pathologies, neuroinflammation and cognitive impairment. The pathological hallmarks of AD are characterized by the accumulation of amyloid-β (Aβ) peptides and phosphorylated Tau (pTau) proteins. However, the expression of Aβ peptides, and Tau proteins levels, as well as the viability of cell in neuronal cells induced by PA remain unclear. Therefore, this study aims to determine the effect of high concentrations of PA on cell viability and Alzheimer’s-related proteins of neuronal cells. In this study, SH-SY5Y was used as a neuronal cell model in order to investigate the effect of PA at a concentration of 400 and 800 µM. The results showed that neuronal cell exposed to PA at doses of 400 and 800 µM for 24 and 48 hours had decreased cell viability in a dose-dependent manner. In addition, receiving PA for 48 hours induced Aβ peptides and p-Tau proteins production. Our study suggests that high concentration of PA exposure can lead to increased risk of the development of Alzheimer’s pathologies and neuronal cell death.

Keywords: Palmitic acid; Alzheimer’s disease; Neuronal cell; Amyloid beta; Tau

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PBT007

In Vitro Cytotoxicity of Cordyceps militaris Extracts on Different Human Cancer Cell Lines

Jitsuda Kullawat1, Pheeraya Chottanom2 and Luchai Butkhup1*

1Natural Antioxidant Innovation Research Unit (NAIRU), Department of Biotechnology, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, Thailand. 2Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, Thailand.

*Corresponding Author: [email protected]

The present study was conducted to evaluate the in vitro cytotoxicity of C. militaris extracted against cancer cells. The cytotoxic activity was tested using Resazurin reduction microplate assay (REMA). The in vitro assay for the C. militaris extracted was measured using three human cancer cells: Human hepatocellular carcinoma (HepG2), Human breast adenocarcinoma (MCF-7), and Cervical adenocarcinoma (HeLa). The REMA assay indicated that Ethyl alcohol (EtOH) and Ethyl acetate (EtOAc) extract of C. militaris exhibited significant cytotoxic affected on MCF-7, HepG-2 and HeLa cancer cells in the dose-dependent manner. C. militaris extracted with EtOAc exhibited stronger cytotoxicity on MCF-7 than HepG2 and HeLa. The results obtained indicated that the probable use of the C. militaris extract in preparing recipes for cancer-related ailments. Further studies will identify bioactive compounds and elucidate more detailed molecular mechanisms of cell death.

Keywords: Codyceps militaris; Cordycepin; Adenosine; Cytotoxicity

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT001

Quality Characteristics of Reduced-Fat Vienna Sausage Using Rice Flour and Skim Milk Powder Mixture as a Fat Replacer

Sakunwiwat, W., Sripui, J., and Rojanakorn, T.*

Department of Food Technology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

*Corresponding Author: [email protected]

The present work investigated the quality characteristics of Vienna sausage containing a mixture of rice flour and skim milk powder (RFSKM) as a fat replacer. Six formulations of Vienna sausage with different amounts of RFSKM (0, 20, 40, 60, 80 and 100%) were produced and evaluated for physicochemical properties and sensory quality. The addition of RFSKM to the sausage significantly increased emulsion stability, viscosity, moisture, and protein content and lowered fat content, calorific value, and cooking loss (p≤0.05). A rise in RFSKM level led to a significant increase in hardness and chewiness and a reduction of lightness of the sausages. Most sensory likability scores of sausage samples with up to 40% RFSKM were comparable to those of the full-fat control sample. The reduced-fat Vienna sausage with acceptable sensory attributes and lower calories can, therefore, be successfully developed with the incorporation of 40% RFSKM.

Keywords: Vienna sausage; Rice flour; Skim milk powder; Fat replacer

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT002

Pro-Inflammation Cytokine Secretion of Peripheral Blood Mononuclear Cells by Edible Mushroom

Pattra Ahmadi Pirshahid1, Sinee Siricoon1,*, Chiramet Auranwiwat1, Wiriyaporn Sumsakul1, Waraporn Sorndech1, Thongkorn Ploypetchara1 and Kwanchai Rattanamanee2

1Expert Centre of Innovative Health Food, Thailand Institute of Scientific and Technological Research, Pathum Thani 12120, Thailand. 2Naresuan University, Phitsanulok 65000, Thailand

*Corresponding Author: [email protected]

Inflammation is a natural process of the innate immune system that associated with the increase in the level of proinflammatory cytokines including tumor necrosis factor alpha (TNFα), interleukin 1β (IL-1β) and interleukin 6 (IL-6). Prolonged inflammation, known as chronic inflammation, related to many chronic diseases such as autoimmune diseases, wound healing, rheumatoid arthritis and cardiovascular disease. The present study aims to investigate the effect of mushroom extracts on the cytokines secretion from lipopolysaccharide (LPS) stimulated peripheral blood mononuclear cells (PBMCs) involved in immune regulation. The secretion of TNF-α, IL-1β and IL-6 was measured by ELISA. The ethanol extract of all mushroom significantly reduced the production of TNF- α. Furthermore, Lentinula edodes, Isaria tennipes, Pleurotus osttreatus and Lentinus squarrosulas Mont. were decreased IL-1β level and Pleurotus osttreatus, Isaria tennipes and Lentinus squarrosulas Mont. were significantly suppressed IL-6 secretion in LPS-treated PBMCs. The application of these extracts showed important anti-inflammatory activity which involved in the treatment and prevention of inflammation and associated diseases.

Keywords: Cytokine; Anti-inflammatory activity; ELISA; Mushroom

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT003

Method Validation for Quantitative Determination of Gallic Acid from Acacia concinna (Willd.) D.C.

Pattra Ahmadi Pirshahid*, Chiramet Auranwiwat, Wiriyaporn Sumsakul, Sinee Siricoon and Waraporn Sorndech

Expert Center of Innovative Health Food, Thailand Institute of Scientific and Technological Research, Pathumthani 12120, Thailand

*Corresponding Author: [email protected]

Gallic acid is one of the most antioxidative components found in Acacia genus. Acacia concinna (Willd.) D.C. belongs to Acacia genus and Fabaceae family, this genus has been used as traditional medicine. The development and validation of RP-HPLC method to determine gallic acid from the ethanolic extract of Acacia concinna. The chromatographic condition was performed on Symmetry Shield RP18 (4.6 x 250 mm) with ACN and 0.5% H3PO4 as mobile phases, flow rate 1.0 mL/min and detection at 272 nm. The quantitative results using external standard, standard gallic acid showed the linear range between 5-50 µg/mL while LOD and LOQ exhibited the values at 0.87 and 2.65 µg/mL, respectively. The accuracy and precision also examined. In the real sample, the ethanolic extract of A. concinna consisted of gallic acid 1.042 %w/w.

Keywords: Gallic acid; Acacia concinna; Method validation

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT004

Effect of Gelation Addition on Physico-Chemical Characteristics of Bastard Oleaster Gummy Jelly

Panida Banjongsinsiri, Nowwapan Donrung and Krittalak Pasakawee*

The Expert Centre of Innovative Health Food (InnoFood), Thailand Institute of Scientific and Technological Research (TISTR) 35 Moo 3 Technopolis, Khlong Luang, Khlong Ha, Pathum Thani 12120 Thailand

*Corresponding Author: [email protected]

Gummy jelly, a gelling-based confectionary product, was prepared by the combination of sugar, gelling agent, and other components. The objective of this present studies was to investigate the effect of different levels of gelation on physico-chemical characteristics of fruit gummy jelly product prepared from Bastard oleaster fruits. Bastard oleaster juice was mixed with water, sugar, citric acid, and the varied concentration of gelatin (at 8%, 9%, and 10% by weight of totally ingredients), then formed in molds and placed at 25C for 2-4 hours until it dried. The physico-chemical characteristics of gummy jelly products were analysed. It was found that pH value, total soluble solid, total acidity, and springiness of gummy jelly products showed statistically significant increase as the increasing of gelatin addition )p≤0.05), in the following ranges, 3.51-3.66, 66.4-69.8Brix, 1.19-1.65% ,8.41-9.19 N, respectively. In contrast, the content of moisture and vitamin C decreased )p≤0.05). Moreover, the increasing levels of gelatin resulted in potentially increased of hardness, gumminess, and chewiness. The results revealed that gelatin addition was effective in improving the physico-chemical texture characteristics of gummy jelly products, especially texture qualities.

Keywords: Bastard oleaster fruit; Fruit gummy jelly; Physico-chemical characteristic

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT006

Development of Ready-To-Drink Powder from Carrot, Orange and Lemon Using Foam Mat Drying

Wattana Wirivutthikorn*

Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Pathumthani 12130, Thailand

*Corresponding Author: [email protected]

Foam mat drying is an economical alternative to drum, spray and freeze drying for the production of food powders. The major advantages of Thai fruits are good sources of bioactive ingredients which can be used as functional ingredients in ready- to- drink beverages. The objective of this research was to study the optimum ratios of carrot, orange and lemon for producing ready- to- drink powder using foam mat drying. Ratio of carrot, orange and lemon was varied into 6 treatments, which were Treatment 1 (control): 100:0:0, Treatment 2: 75:20:5, Treatment 3: 70:20:10, Treatment 4: 50:40:10, Treatment 5: 50:45:5 and Treatment 6: 62:31:7. The results showed that color (L*, a*, b*) and solubility of samples in all treatments were not significant different. The study chemical of measurement as pH, moisture, total soluble solid, vitamin C, - carotene and antioxidant were performed. The results showed that Experiment 4 had the highest antioxidant and -carotene values of 3.68 mg Trolox eq/g and 0.14 µg/100g FW, respectively. The sensory evaluation on taste and overall acceptability by using 9- point hedonic scale revealed that panelists accepted Experiment 4 which gave the highest scores of 5.70 and 6.33, respectively. From the information obtained, the formulation of powdered ready to drink produced from Thai fruits can be developed to be accepted by consumers, which has expanded in the large future level in beverage industry.

Keywords: Carrot; Foam mat drying; Lemon; Orange; Powder

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT007

Efficacy of Gamma Irradiation on Sprout Inhibition, Insect Infestation and Antioxidant Activity of Fresh Black Galingale (Kaempferia parviflora) for Prolonged Storage

Panchalee Prakongsil1,*, Thitima Kongrataporn1, Surasak Sajjabut1, Todsapol Tanrin1, Wachiraporn Pewlong1 and Nuatawan Thamrongsiripak2

1Research and development section, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok, Thailand. 2Irradiation Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok, Thailand.

*Corresponding Author: [email protected]

A study on the method for improving shelf life and phytochemical activity of fresh black galingale rhizome (Kaempferia parviflora) or Krachai-dum in Thai from Phetchabun province, lower northern of Thailand by gamma irradiation at dose of 180 Gy in prolonged storage at ambient temperature (33.0+1.0C, 52.5% to 66.5% relative humidity) were investigated. This study explained how the use of gamma radiation has beneficial application in agriculture. The results showed that gamma irradiation at dose of studied could effectively inhibit sprouts and decrease weight loss of fresh rhizome without any propagation of insects for longer than 60 days. The level of total phenolic content (TPC), DPPH scavenging activities (DPPH) and ferric reducing potentials (FRAP)of irradiated rhizome in prolonged storage were ranged from 1.86+0.22 - 1.68+0.13 mg gallic acid equivalent/g, 0.61+0.17 - 0.54+0.11 mg ascorbic acid equivalent/g, 9.18+0.98 - 9.35+0.86 µmol FeSO4/g, respectively. There were no significant differences in TPC, DPPH and FRAP between non-irradiated samples and the irradiated samples for all of storage time. The results revealed that this method is an alternative for sprout inhibition, could be extended shelf life within remaining and keeping in antioxidant activity of fresh herbal black galingale rhizome for prolonged storage.

Keywords: Gamma; Sprout inhibition; Insect infestation; Antioxidant activity; Black galingale

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT008

Investigation the Physical, Mechanical Properties of Edible Film from Riceberry Flour

Walaiporn Hemso*, Wannee Suttivattanavet and Thongkorn Ploypetchara

Expert Center of Innovative Health Food, Thailand Institute of Scientific and Technological Research 35 Mu 3 Technopolis, Tambon Khlong Ha, Amphoe Khlong Luang, Pathum Thani 12120, Thailand

*Corresponding Author: [email protected]

Edible film samples containing riceberry flour at 15, 20, 25 and 30% (w/w) with adding glycerol as a plasticizer were extruded. The film properties were investigated. The results showed that the thickness and opacity of film samples decreased while moisture content, aw, and water solubility increased with an increase in the concentration of glycerol. The colors (L, a*, b*) values of the films were changed with glycerol concentration. The mechanical properties in terms of tensile strength and load at the maximum of the film decreased but elongation at break increased with an increase in the concentration of glycerol. Adding glycerol as a plasticizer affected the physical and mechanical properties of riceberry film depending the concentration of glycerol.

Keywords: Riceberry; Film; Glycerol; Physical properties; Mechanical properties

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT009

Effect of Storage and Packaging Conditions on Physical Properties and Carotenoid Content of Mango Tablet

Thorung Pranil1, Anuchita Moongngarm1,*, Greepol Manwiwattanagul2 and L. Kerr William3

1Research Unit of Nutrition for Life, Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand 2Faculty of Pharmacy, Mahasarakham University, Maha Sarakham 44150, Thailand 3Department of Food Science and Technology, University of Georgia, 100 Cedar Street, Athens, GA 30602-2610, USA

*Corresponding Author: [email protected]

Storage conditions play an important role in the physical and chemical stability of food tablets. Mango tablets were prepared and then stored under controlled in either polyethylene or laminated plastic bags with relative humidity of 32 and 75 % at temperatures of 4, 25, and 35 °C for six months. Moisture content, water activity, carotenoid content, weight, shape, color, and texture were regularly assessed. Both storage conditions and storage time influenced mango tablet properties. Combination of high temperature (35 °C), high relative humidity (75 %) and packaging in polyethylene plastic bags significantly increased moisture content and water activity of mango tablets. This led to changes in other properties including decreased carotenoid content, diameter, thickness, L*, b* and hardness, and also increased weight, a* and fracturability. No significant changes were observed in tablets packed in laminated plastic bags (non-permeable to oxygen and humidity) irrespective of temperature and relative humidity. Results showed that mango tablet stability was influenced by packaging and storage conditions.

Keywords: Stability; Mango tablet; Temperature; Relative humidity; Packaging

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT010

Impact of Lactic Acid and Hot Water Treatment Combined on Quality Change of Storage Shucked Hard Clam (Meretrix casta)

Supraewpan Lohalaksanadech* and Chutinut Sujarit

Department of Food Industry and Fishery Product, Faculty of Science and Fishery Technology, Rajamangala University of Technology Srivijaya, Trang Campus,Trang 92150, Thailand

*Corresponding Author: [email protected]

This research aimed to investigate the quality and shelf life extension of shucked hard clam (Meterix casta) dipped into hot water and lactic acid. The samples were treated with hot water and lactic acid (LA) combined with various concentrations as follow: 1) control sterile tap water, 2) dipped into hot water at 65ºC followed by dipping in 2.0%LA 3) dipped into the 2.0% LA alone respectively. The samples were dipped into the hot water for 30 s. followed by dipping in LA for 30 s. The results indicated that the dipping in hot water and followed by dipping in LA was efficient against the proliferation of microorganisms including total variable count, the inhibitory effect on bacterial growth was pronounced when the temperature of hot water increased. The results from chemical analysis indicated that the treated samples underwent a significant decrease (P<0.05) in the term of pH value and total volatile base nitrogen content. However, the results of sensory attributes show that the high scores for the color, texture and odor demonstrated that dipping in hot water at 65ºC followed by dipping in 2.0% LA solution can help delay the proliferation of microorganisms spoilage, prevent the generation of undesirable chemicals, improve the levels of sensory attributes and extend the shelf life of shucked hard clam during refrigerated storage.

Keywords: Hard Clam (Meretrix casta); Total volatile base nitrogen; Shelf life; Lactic acid; Hot water, Dipping

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT011

Optimization of Pulsed Vacuum Osmotic Dehydration of Model Food and Application for Important Compounds Enrichment

Kulab Sittisuanjik1, Pheeraya Chottanom1,*, Anuchita Moongngarm1, and Sirirat Deeseenthum2

1Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, Thailand 2Department of Biotechnology, Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

Pulsed vacuum osmotic dehydration (PVOD) is an application of vacuum pressure at the beginning of osmotic dehydration (OD) in food processing. The objectives of this study were to apply response surface methodology for optimizing of PVOD process to achieve sufficient solids in a gel matrix using a diluted OD solution. Its application for the enrichment of antioxidant compounds and nutritive compounds was also investigated. The process variables were agar-gel bulk density (0.65-0.85 g/ml), sucrose concentration (30-60 % w/w) and vacuum impregnation time (10-30 min). The pulse vacuum was applied at 150 mbar followed by an atmospheric pressure operation up to 180 min. The results showed that the process variables, especially the concentration contributed to moisture content, water loss, solid gain, weight reduction and total soluble solids. A quadratic model was fitted for WL, SG, WR and TSS prediction (푅20.997−0.999). The experimental verification was adequately reproducible for all responses variables. The prediction of TSS was the most precise (푥20.095). Optimum conditions were 32.58 %w/w, 0.8 g/ml and 14.34 min of sucrose content, bulk density and time, respectively. The physiological compounds could be sufficiently introduced into the model food with a diluted solution. The results can be applied to the development of new innovative products that provide the desired element level and functionality according to the consumer requirements.

Keywords: Functional food; Mass transfer; Osmotic dehydration; PVOD; Response surface methodology

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT012

Production of High Melatonin and Antioxidant Activity Mungbean Sprout by Germination of Mungbean Seeds under Salinity and Exogenous Melatonin

Anuchita Moongngarm1,*, Arisa Chaiyarak2, Kanyapuk Prachanun2, and Supap Nontasan1,2

1Research Unit of Nutrition for Life, Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand 2 Department of Food Science and Technology, University of Georgia, 100 Cedar Street, Athens, GA 30602-2610, USA

*Corresponding Author: [email protected]

Melatonin is a natural hormone produced by the pineal gland in the brain to regulate human circadian rhythms. It is often used to treat sleep disorders and also as an antioxidant. High melatonin and antioxidant mungbean sprouts were produced. Effects of germination time, sodium chloride (NaCl) stress, exogenous melatonin treatment on improvement of endogenous melatonin, total phenolic compounds (TPC) and antioxidant profiles of mungbean sprouts were evaluated using response surface methodology (RSM). Eleven conditions of germination were generated using fractional factorial design. Germination characteristics, endogenous melatonin content, TPC and antioxidant profiles of sprouts were determined. Results indicated that all conditions led to over 92% germination rate. Increase in germination time, NaCl concentration and exogenous melatonin resulted in elevation of endogenous melatonin, TPC and antioxidant activity of sprouts. Optimal conditions for germination of mungbean sprouts were 0.50% NaCl, 30 µM melatonin and germination time 72 h. Predicted values under these conditions were 958.92 ng/g for melatonin, 1.90 mg/g for total phenolic content, 0.99 mg TE/g, 0.65 mg VCE/g, 0.30 mg GAE/g for DPPH radical scavenging assay, and 10.86 mg TE/g, 5.19 mg VCE/g and 101.61 mg GAE/g for total antioxidant capacity. Results suggested that germination under salinity and exogenous melatonin significantly improved melatonin content, total phenolic compounds and antioxidant activity. Germination under these conditions is considered a potential process that could be adopted to produce ready to eat sprouts or functional ingredients with high concentration of melatonin for the food industry.

Keywords: Melatonin; Antioxidant activity; Mungbean; Germination; Phenolic compounds

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT013

Unripe Banana Flour Improved Resistant Starch Content and Quality Characteristics of Gluten-Free Rice Cookies

Pakkawat Detchewa1,*, Patcharee Prasajak1, Wichien Sriwichai2 and Anuchita Moongngarm3

1Department of Agro-Industry Technology and Management, Faculty of Agro-Industry, King Mongkut’s University of Technology North Bangkok, Prachinburi Campus, Thailand 2Department of Innovation and Product Development Technology, Faculty of Agro-Industry, King Mongkut’s University of Technology North Bangkok, Prachinburi Campus, Thailand 3Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Thailand

*Corresponding Author: [email protected]

Rice (Oryza sativa L.) as one of the most important global cereals is considered to be a gluten-free food. However, using rice flour to prepare cookies still has several limitations such as poor texture, poor color, low sensory quality acceptance by consumers and high glycemic index. Effects of banana flour substitution on resistant starch (RS) contents and qualities of gluten-free rice cookies were investigated. Gluten- free rice cookies were replaced with banana flour at 0, 50, 60, 70, 80 and 100% dry basis (db). Physical properties, pasting properties, textural properties, resistant starch content and sensory evaluation of the cookies were determined. As the degree of banana flour substitution increased, peak, breakdown, final and setback viscosities of blended flour samples also increased. Physical properties including spread ratio, color, and texture of gluten-free rice-based cookies improved when rice flour was replaced by unripe banana flour. Resistant starch content of gluten-free rice cookies ranged from 1.90 to 8.50% with replacement by banana flour, while wheat cookies contained 2.85% resistant starch. Gluten-free rice cookies were also subjected to sensory evaluation. Results indicated that gluten-free rice cookies with 70% unripe banana flour gave highest overall scores and were comparable to wheat cookies. Partially replacing rice flour with unripe banana flour has the potential to produce gluten-free rice cookies with high RS content.

Keywords: Gluten-free; Rice cookie; Wheat cookie; Resistant starch; Unripe banana flour

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT014

Physicochemical Properties of Dried Guizhou as Affeced by Pretreatments, Drying Techniuques and Storage Period

Chanthima Phungamngoen1,*, Pakkawat Detchewa1, Yardfon Tanongkankit2 and Anuchita Moongngarm3

1Department of Agro-Industry Technology and Management, Faculty of Agro-Industry, King Mongkut’s University of Technology North Bangkok, Prachinburi Campus, Thailand 2Department of Agricultural and Food Engineering, Faculty of Engineering and Agro- Industry, Maejo University, Thailand 3Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Thailand

*Corresponding Author: [email protected]

Guizhou (Artemisia lactiflora) is a widely consumed vegetable worldwide that contains high concentration of phenolic acids and flavonoids, and demonstrates antioxidant activity. Guizhou is normally processed and stored as dried leaves which affects the concentration of bioactive compounds and physical properties. The effects of pretreatment methods (hot water blanching, steam blanching and acetic acid soaking) and drying techniques (hot air drying, vacuum drying and freeze drying) on the physical properties and antioxidant activities of dried Guizhou were investigated. Results showed that blanching improved the drying rate. Blanched samples dried faster than untreated and acetic acid treated samples. Vacuum drying gave slower drying rates than hot air drying. Samples dried by hot air drying and vacuum drying exhibited more shrinkage (wrinkles) than those subjected to freeze drying. Dried blanched samples had greener and darker color than acetic acid pretreated and untreated dried samples. Freeze dried samples had highest total phenolic compounds and antioxidant activity determined by DPPH assay. Moisture content, water activity, E of hot air dried, vacuum dried and freeze dried samples gradually increased throughout the 6 month storage period, while total phenolic compounds and DPPH radical scavenging activity showed a decreasing trend. Aluminum foil provided better protection against moisture uptake by dried samples than polypropylene containers. Freeze dried samples kept in aluminum foil at room temperature gave the longest shelf life. Results suggested that pretreatment by blanching, suitable drying methods and aluminum packaging improved physical properties and protected phenolic acid content of dried Guizhou.

Keywords: Pretreatment; Guizhou; Drying method; Antioxidant; Shelf life

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PFT015

Formulation Optimization of Purple Rice-Coconut Milk Drink Based on Physicochemical Properties using Mixture Design

Tatdao Paseephol*, Chonticha Khahakhasit, Chalida Chaisuwan, and Wanida Chuenta

Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

This study aimed to determine the optimum formulation of purple rice-coconut milk drink. Extreme vertices design of mixture experiments for the three components was performed. The proportions of the three components were 20.00-28.78% boiled purple rice (BPR), 10.00-18.78% grated coconut meat (GCM) and 55.00-63.78% warm water (WW) equivalent to a total of 93.78%. Four formulations were generated. The experimental data were analyzed using analysis of variance (ANOVA) and were fitted to a linear model equation. The results revealed that the increase in BPR produced a significant increase in antioxidant capacity, total phenolic content (TPC), total anthocyanin content (TAC), and the redness of the coconut milk drink. Besides, an increase of GCM caused greater values for TPC and L* and lower value for a* value. The physicochemical properties of the drink were least affected by WW. The statistically significant predictive models with satisfactory coefficients of determination (R2> 0.75) were used to plot the contour plot and determine the optimum values of responses. It was found that a formulation containing 27.92% BPR, 10.86% GCM, and 55.00% WW led to the desirable physicochemical properties with combined desirability equals to 0.753. Confirmatory trial results achieved reasonably close between the observed and predicted values. The data presented could be useful in developing a plant- based functional drink for health-conscious consumers.

Keywords: Coconut milk; Black glutinous rice; Mixture design; Gum arabic

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT001

Development of Rice Quality to Good Agricultural Practice Standards of Farmers in Mukdahan Province

Suwit Pengkeaw*

Department of Agriculture and Cooperatives Sukhothai Thammathirat Open House, Mukdahan 49000, Thailand

*Corresponding Author: [email protected]

The purpose of this research is to study the basic personal, social, and economic conditions. Knowledge and sources of knowledge about rice production in accordance with the Good Agricultural Practices Standard (GAP), rice production in accordance with GAP standards, opinions of farmers who want to promote GAP standards and problems, recommendations and procedures for promotion and development of rice quality to GAP standards for farmers. The sampling procedure was 145 people from 300 population by simple sample selection using interview form. Data were analyzed using descriptive statistics. The results showed that (1) Most of the farmers were female, with an average age of 55.70 years and an average of 2.76 household laborers. The average participation area of the project was 7.81 rai. (2) Farmers had knowledge about GAP standards for rice. High level Get the knowledge from the agriculturalists at the highest level. (3) All farmers grow rice by using rain water. Soil preparation by plowing 2 times, rake 1 time 89.7%, sowing rice fields 62.1%, planting rice with RD 6 and 86.2% using labor and harvesting rice together. The average yield of rice after participating in the project is 445.03 kilograms per rai. (4) Farmers have the highest level of opinions and demand for rice according to GAP standards. The opinions of rice quality development in accordance with GAP standards are at the highest level. Opinions about the promotion of rice production in accordance with GAP standards are at a high level. The opinion about the motivation for rice production according to the GAP standard was at the highest level. The opinion about the need to promote knowledge about rice production in accordance with GAP standards is at the highest level. (5) Most farmers have problems with lack of funds for rice production due to high rice production costs and farmers lacking training and demonstration. The suggestion is that the government agencies should support the production factors sufficiently and should actually implement every step.

Keywords: Rice production; Standards; Good agricultural practices; Mukdahan province

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT002

Potential of Bitter Bush against Maize Weevil (Sitophilus zeamais)

Ruchuon Wanna*

Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding Author :[email protected]

Maize weevil (Sitophilus zeamais Motschulsky), is an important insect pest of seeds and grains in the silo that stores agricultural products. In order to control this insect, synthetic insecticides are used. However, it has side effects to human health and environment. Therefore, using of substances or products from natural plants that are easily disintegrated is a good choice. The aim of this research is to study the potential of local natural plants in the saline soil area of forest community at Ban Na Si Nuan, Na Si Nuan subdistrict, Kantharawichai district, Maha Sarakham province, Thailand to control maize weevil. Chemical compositions of plant powder from Bitter bush (Chromolaena odorata), Wild daisy (Tridax procumbens) and Little ironweed (Cyanthillium cinereum) were analysed by using Gas Chromatograph-Mass Spectrometer (GC-MS). Data on the amount of F1 maize weevil adult and shelf life of F1 maize weevil adult were collected. The results showed that Bitter bush powder contained 90 compounds and hexanal (7.73%), alpha- guaiene (3.61%) and alpha-pinene (2.27%) were the main components. Bitter bush powder

10 g/100 g grains was the best efficiency to control the amount of F1 maize weevil adult.

The inhibition percentage of F1 maize weevil adult emergence was 45% and grains of Khao Dawk Mali 105 rice were destroyed less than 4%. Bitter bush powder 10 g/100 g grains was the best efficiency to protect the damage of Khao Dawk Mali 105 rice from the adult of F1 maize weevil. It could work as a residue to protect the adult of F1 maize weevil for

14 days. There was no adult of F1 maize weevil and Khao Dawk Mali 105 rice grains was not damaged.

Keywords: Natural plant products; Weeds; Chemical compositions; Stored insect pests; Rice

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT003

The Nutritive Value and Bioactive Compounds of Alfalfa (Medicago sativa) Grown at Burapha University, Sa Kaeo Campus

Supreena Srisaikham and Quanjai Rupitak*

Faculty of Agricultural Technology, Burapha University, Sa Kaeo Campus, Sa Kaeo 27160, Thailand

*Corresponding Author: [email protected]

Legume is a good quality protein source that provides nutritive value for livestock animals, particularly ruminants. It also contains bioactive compounds which can be work against oxidants that prevent diseases. Alfalfa (Medicago sativa) is a perennial flowering plant in the legume family Fabaceae with a high protein content and moderate contents of vitamin B, C, phosphorus, and zinc. The aim of this research was to evaluate the nutritive value and bioactive compounds of alfalfa at harvesting intervals of 120 days at Sa Kaeo province. Alfalfa was grown at the agricultural research facility of the Faculty of Agricultural Technology, Burapha University, Sa Kaeo Campus, Thailand. They experiment was done from September 2018–March 2019. Chemical composition and bioactive compounds (tannin, total phenolic, total flavonoids, and isoflavone) were measured. The results showed that dry matter, ash, crude protein, fat and crude fiber were 91.44%, 9.20%, 17.68%, 2.46%, and 26.48%, respectively. Whereas, tannin, total phenolic, total flavonoids, daidzein and genistein in dry alfalfa were 2.34%, 2.17 mg RE/g sample, 2.02 mg RE/g sample, 46.72 and 15.38 µg/g sample, respectively. The highest phenolic compounds were 203.60 µg/g sample. The results suggest that the alfalfa plant can be developed as supplement that is benefit to health, for dairy cattle to achieve alternative products that contains high-flavonoids level in goat or dairy cow’s milk that will be useful for ruminant and human health as well.

Keywords: Legumes; Alfalfa; Medicago sativa; Nutritive value; Bioactive compound

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT004

Effect of Pectic Oligosaccharides from Fruit Peels as Prebiotic in Animal Feed

Pornpan Saenphoom*, Pattaraporn Poommarin, Suphavadee Chimtong, Wasunan Nimanong and Sudarat Artkidkan

Faculty of Animal Science and Agricultural Technology, Silpakorn University, Phetchaburi 76120, Thailand

*Corresponding Author: [email protected]

The objective of this study was to improve fruit peels (pomelo peel, lime peel and mango peel) with enzyme (Hemicell®) as prebiotics in animal feed. There were 2 experiments designed in completely randomized design (CRD). In the experiment 1, it consisted of 6 treatments with 3 types of fruit peels (pomelo peel, lime peel and mango peel) and 2 levels of enzymes (0 and 1% (w/w)) with 3 replications. The samples were taken to measure chemical compositions, reducing sugar content and oligosaccharides analysis. The results were showed that chemical compositions were significantly different among treatments (P<0.01). Enzyme treated lime peel had higher ash, crude protein and ether extract content but lower cellulose than other treatments (P<0.01), 4.98, 6.40, 8.87% and 9.92%, respectively. Enzyme treated and untreated mango peel contained higher gross energy than other treatments (P<0.01), 4,100.54 and 4,069.14 kcal/kg, respectively. In addition, reducing sugar content was significantly different among treatments (P<0.05). Enzyme treated mango peel contained higher reducing sugar content than other treatments (P<0.05). There were 42.04, 28.86, 177.06, 77.28, 37.09 and 265.91 mg/g, respectively. Oligosaccharides analysis by Thin layer chromatography method showed that all treatments releasing oligosaccharides. In experiment 2, it was examined prebiotic properties (concentration of sugar 1.5 mg/ml), the results showed that glucose product from all treatments could increase growth of Lactobacillus plantarum but enzyme treated lime peel could decrease growth of Escherichai coli. In conclusion, lime peel treated enzyme can be used as prebiotics as it could increase probiotics and decrease growth of pathogenic bacteria.

Keywords: Fruit peels; Enzyme; Oligosaccharides; Prebiotics

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT005

Toxicity and Bioactivity of Powder and Essential Oil of Climbing Wedelia against Maize Weevil

Ruchuon Wanna*, Darika Bunphan, Waranyoo Kaewduangta, Mongkol Wongsawas and Phirayot Khaengkhan

Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

Many powders and essential oils from plant products are used for traditional protection of stored products against insect pests. These are relatively non-toxic to mammals and meet the criteria for reduced risk pesticides. Toxicity and bioactivity of powder and essential oil from the climbing wedelia, Wedelia trilobata (L.) A.S. Hitchcock were determined against maize weevil, Sitophilus zeamais Motschulsky. Powder and essential oil were obtained from the aerial parts of W. trilobata and tested for their toxicity, insecticidal and residual activities against S. zeamais. The LC50 value of W. trilobata powder to S. zeamais was 582.86 g/kg grain within 72 hours, while essential oil was 1,146.19 μL/L air within 24 hours. Mixing powder with grain at a dosage of 80 g/kg grain killed S. zeamais at 7.50% after 12 days of exposure and reduced progeny of S. zeamais by 89.96%. After fumigation, concentrations of 150, 200 and 250 μL/L air of W. trilobata essential oil showed 2.50, 5.00 and 12.50% adult mortality of S. zeamais after 24 hours of exposure with reduction in progeny of 81.97, 83.07 and 97.65%, respectively. Powder and essential oil of W. trilobata products showed efficacy for newly emerged protection of progeny of S. zeamais (>80%). W. trilobata essential oil exhibited a moderate to strong residual activity. Results suggest that powder and essential oil from W. trilobata may be effectively used in the management of S. zeamais populations in grain storage.

Keywords: Plant products; Toxicity; Insect pests; Insecticidal activity; Residual activity

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT006

Effects of Alternate Wetting and Drying Techniques on Grain Yield and Water Use Efficiency in Irrigated Rice

Surasit Punyawansiri1,*, Kantasinee Chaengpui2 and Bancha Kwanyuen1

1Department of Irrigation Engineering, Faculty of Engineering, Kasetsart University, Nakhon Pathom, Thailand 2Phitsanulok Irrigation Water Management Research Station, Royal Irrigation Department, Phitsanulok, Thailand.

*Corresponding Author: [email protected]

Alternate wetting drying method (AWD) is considered as a water management technique to reduce the water use in paddy field. The effect assessment of AWD on plant growth, yield and water management in Thailand is still in the preliminary stage and few studies have been conducted in this region. The objective of this study was to determine whether the AWD technology could maintain grain yield with reduced water input as compared to the conventional farmer’s practice or continuous flooding method (CF) in north of Thailand. The implementation of water management strategies under AWD was tested in comparison with CF. The experiment was conducted during dry season in 2016 and 2017, in the irrigated rice fields using Phitsanulok-2 rice in Phitsanulok irrigation district. From the results, it was found that the water input to the plots with AWD techniques used less water than the continuous water supply method at approximately 27.20 % in 2016 and about 18.20 % in 2017 in the dry seasons and the average water productivity (WP) of AWD and CF were 14.83 and 11.75 (kg/m3) of paddy, respectively. Therefore, the water-saving irrigation techniques by AWD is the appropriate water management strategy for cultivation of irrigated rice and it is an effective way to reduce of water used in the agricultural sector and methodologies used were adequate to support irrigation management advising for farmers.

Keywords: Water management; irrigation; AWD; Paddy yield; Phitsanulok-2 rice

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT007

Sprout Contain Higher Phenolic, Flavonoids, and Antioxidant Activity than Seed in Different Thai Varieties of Mungbeans (Vigna radiata L.)

Maratree Plainsirichai*, Chutima Khramsungnoen and Jiraporn Krasaetep

Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Khamring, Kantarawichai, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

It has been reported that mungbean seed contained fat, protein, lecithin, and amino acid while the study on antioxidants and antioxidant activity in seed particulary comparing to sprout of Thai mungbean varieties is rare. This research studied antioxidants and antioxidant activity in seed and sprout of 5 varieties of Thai mungbean (Vigna radiata L.), cv. Chainat 36, Chainat 72, Chainat 84-1, SUT1 and KPS 2.Total phenolic, total flavonoids, chlorophyll a, b, total chlorophyll, total carotenoids and antioxidant activity (DPPH) in seed and sprout were analyzed. The experiment was arranged in CRD (Completely Randomized Design) with 4 replications. The results demonstrated that seed of mungbean variety Chinat 36, 72, 84-1, contained the highest concentration of total phenolic at 50.57-57.47 mg GAE/100 g dry weight. Sprout of mungbean cv. Chainat 36 contained the highest total flavonoids, chlorophyll a, b, total chlorophyll and antioxidant activity (3,372.8 mg GAE/100g of DW, 6,808.6 mg QE/100g of DW, 0.38 µg/mL, 1.20 µg/mL, 1.58 µg/mL and 0.90 µg/mL, respectively). Sprout of mungbean at day 4 of every mungbean variety contained significantly higher total phenolic, total flavonoids and antioxidant activity than other germination stages and higher than those in seed. In conclusion, antioxidant and antioxidant activity in seed were low and there was low variation among mungbean seed varieties. During germination germination especially at day 4, total phenolics, total flavonoids, and antioxidant activity were very high particulary in mungbean CV. Chainat 36. Therefore it could be suggested that sprout of mungbean CV. Chainat 36 at day 4 was best for consumption as total phenolic content and total flavonoid content were almost 10 and 15 fold respectively of seed.

Keywords: Phenolic; Flavonoid; Antioxidant activity; Mungbean; Seed; Sprout

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT008

The Comparative of Growth and Carcass Performance in Native Chickens (Chee): KKU 12 and Chee N

Doungnapa Promket* and Khanitta Pengmeesri

Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Thailand

*Corresponding Author: [email protected]

The objective of this study was to compare growth and carcass performance of native chickens (Chee): between population selected for economic traits (Chee KKU12) and population of natural selection (Chee N). Thai native chickens are most popular local breed for rural people because its relative with adaptation in community. The native chicken (Chee) being developed to be more suitable for commercial production, which may affect the natural behaviour of native chickens. The completely randomized design (CRD) was used to analyse growth and carcass performance. Two hundred of Chee KKU12 and Chee N were divided into 5 replicates, 20 chickens per replication. Weigh every 2 weeks for 12 weeks. Male and female chickens were randomized from each replication to evaluate carcass quality. The results showed that during 0-12 weeks of age, Chee KKU12 (1,246.78 g) had higher body weights more than Chee N (1,148.13 g). In addition, the body weight when chickens aged 6, 8, 10 and 12 weeks in Chee KKU12 were higher than Chee N. Average dairy gains of Chee KKU12 were higher than Chee N. However, feed intake and feed conversion ratio were not different. The carcass quality was not different among groups. Weight of abdominal fat and gizzard of Chee KKU12 were higher than Chee N, especially in male. The result can be concluded that the Chee KKU 12 grew rapidly in open housing conditions, but the abdominal fat was higher than the Chee N.

Keywords: Growth performance; Carcass quality; Chee KKU 12; Chee N

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT009

The Effect of Oil and Non Structural Carbohydrate (NSC) Sources in Different Ratios of Roughage to Concentrate in Total Mixed Ration (TMR) Diet on Total Gas Production and Nutrient Digestibility on In Vitro System

Sirisan, S.*, Kenchaiwong, W., Pongthaisong, P., Piratae, S., and Ketphonthong, S.

Facultry of Veterinary Science, Mahasarakham University, Maha Sarakham 44000, Thailand

*Corresponding Author: [email protected]

Oil and non-structural carbohydrate (NSC) are the energy sources in different roughage proportion in the diets had important for digestibility and utilization of feed for ruminants. Gas production technique is a reflected to the rate of extent of dry matter digestion. Digestibility and gas production on in vitro systems could be evaluated the initial utility of diet before feeding to animals. Therefore, the aim of this study was to determine the several of oil and NSC source in different ratio of roughage in total mixed ration (TMR) diet on in vitro dry matter digestibility (IVDMD), in vitro neutral detergent fibre digestibility (IVNDFD) and total gas production. The experimental design was conducted by completely randomized design (CRD). The in vitro study was applied to determine IVDMD, IVNDFD and total gas production from eight experimental diets as follow: two source of oil (soybean oil and palm oil), four source of NSC (sugar, starch, cassava chip and corn meal) in several level of roughage as 30, 40, 50 and 60% of TMR diet. The results revealed that soybean oil and palm oil combination with starch, cassava chip and corn meal at the proportion of roughage of 30, 40 and 50% had highly significant improved IVDMD and total gas production but IVNDFD was decreased. Using oil combination with sugar is easily ruminal carbohydrate in high proportion of roughage as 60% in TMR diet; it does not improve nutrient digestion and total gas production. Therefore, this study suggested that can be used soybean oil, palm oil together with NSC sources, from starch, cassava chip and corn meal but must be in a roughage proportion that is not more than 50% in the TMR diet are resulting in increased IVDMD and total gas production.

Keywords: Oil; Non-structural carbohydrate (NSC); Digestibility; Gas production, in vitro technique

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT010

Effect of Dietary Papaya Peel and Baker’s Yeast Supplementation on Growth Performance, Hematological Value of Silver Barb (Barbonymus gonionotus) and Its Resistance to Aeromonas hydrophila

Mahattanee Phinyo1,2,*, Areeya Thaikosa1 and Eakapol Wangkahart3

1Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand 2Center for Agricultural Biotechnology, Naresuan University, Phitsanulok 65000, Thailand 3Division of Fisheries, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

The aim of this study was to investigate the effects of dietary papaya peels and baker’s yeast (Saccharomyces cerevisiae) supplementation on the growth, hematological value of Silver barb (Barbodes gonionotus) and disease resistance post A. hydrophila infection. The study comprised of four experimental diets, namely a control diet (C), 4% (w/w) papaya peel (P), 2% (w/w) baker’s yeast (Y), and 4% (w/w) papaya peel/2% (w/w) baker’s yeast (PY). After 8 weeks of feeding, silver barb fed with PY had significantly higher values of final body weight (14.53±0.29 g/fish) and specific growth rate (SGR, 1.87±0.05 %/day) compared to the control group (P<0.05). They also recorded improved feed conversion ratio (FCR) (P<0.05). However, the C and P diets did not exhibit significant differences in the FCR and SGR. During the feeding trial, no mortality was observed in any of the fish fed with the experimental diets. Silver barb fed PY had significantly higher red blood cells (RBC) and white blood cells (WBCs) than those in other diet groups (P<0.05). Intraperitoneal injection of 0.1 ml (0.5x107 CFU mL-1) of Aeromonas hydrophila into the silver barb resulted in increased mortality, that was significantly decreased with the intake of dietary baker’s yeast (P>0.05). In conclusion, the diet group with compositions of 4% papaya peel (P) showed no differences in growth performance and hematological parameters in the silver barb when compared with the control (C) group. However, the dietary inclusion of baker’s yeasts improved the growth performance, as well as resistance to A. hydrophila. These results indicate that papaya peel and baker’s yeast can be used as a feed additive for silver barb that might yield great economic benefits.

Keywords: Baker’s yeast; Silver barb; Papaya peels; Growth; Disease resistance

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT011

The Effect of the Seasons to the Changes of Water Quality and Sediment Quality in the Coastal Area of the Andaman Sea in Trang Province, Thailand

Dumrong Lohalaksanadech*, Porntep Virachawong, Chutinut Sujarit and Krisada Pramchuaim

Department of Food Industry and Fishery Product, Faculty of Science and Fishery Technology, Rajamangala University of Technology Srivijaya, Trang Campus, Trang 92150, Thailand

*Corresponding Author: [email protected]

This study is to investigate the changes of water quality and the sediment as well as the change of coliform bacteria in the coastal area of the Andaman Sea in Trang province, Thailand. The information gained from the study would be the way to prevent and manage the aquaculture system in the coastal area. The analysis of water quality was conducted by examining pH value, salinity, temperature, and dissolved oxygen. Also, the analysis of the sediment quality was carried out by examining pH value, electrical, conductivity, salinity, and the amount of organic matter. The groups of coliform bacteria were total coliform bacteria and fecal coliform bacteria. Water and sediment samples were collected from 8 stations with three examines at the same places during the months of January through to December 2017. The samples were collected once a month for 12 times. The result showed that the quantities of coliform bacteria of water samples during the hot and rainy seasons were 3.097±5,360.49 MPN/100 ml and 2182.55±3004.83 MPN/100 ml respectively, and the quantities of fecal coliform were equivalent to 1,625.12±3,204.03 and 2,835.46±4,635.20 MPN/100 ml. The result of physical and chemical water qualities found that the values of water temperature, the amount of dissolved oxygen, pH value, and conductivity in both hot and rainy seasons were indifferent. During the hot season the values of parameter were a little bit higher than in the dry season. The value of water salinity in the hot season was higher than in the rainy season. The results of sediment quality indicated that both coliform bacteria and fecal coliform bacteria in the rainy season was less than in the hot season. The analysis of physical and chemical sediment qualities examining the conductivity and the amount of organic matter during the hot season showed that the values of conductivity and the amount of organic matter were higher than in the rainy season. The analysis of the pH value showed no difference in both seasons. Therefore, the results of this study concluded that water and sediment qualities were suitable for aquaculture. Besides, coliform bacteria and fecal coliform contaminated more in water than in the sediment, especially in the hot season. Therefore, during the rainy season, there is a high risk of having aquatic animals that contaminated with coliform bacteria.

Keywords: Coastal; Water quality; Sediment; Coliform bacteria; Fecal

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT012

Microplastic Contamination Assessment in Freshwater Bivalves: A Case Study of Ubolratana Reservoir

Pattira Kasamesiri1,2,* and Wipavee Thaimuangphol1,2

1Faculty of Technology, Mahasarakham University, Thailand 2Research Unit of Excellence for Tropical Fisheries and Technology, Mahasarakham University, Thailand

*Corresponding Author: [email protected]

Microplastic pollution are widely spread in ocean and freshwater ecosystem but studies of plastic contamination in freshwater ecosystems remain limited. Contamination of microplastics in a commercial freshwater bivalve is an important issue which leads to human health risk. Common freshwater bivalves in the Ubolratana Reservior, Thailand were investigated for abundance, size, color and shape of microplastics. Five freshwater bivalve species were observed. Results showed that 519 particles were found in freshwater bivalves and 100% of the collected samples were polluted with microplastics at mean abundance of 2.661.39 particles per individual. The abundance of microplastics between species was significant difference (P<0.01). Abundance of microplastics was highest in Corbicula siamensis (3.151.53 particles per individual) with the most common size of microplastics filtered by bivalves at over 0.5 mm (65%), of which 55% were blue color and 83% were fiber shaped. Results revealed that fishing gear made by nylon may a major sources of microplastic contaminants in the Ubolratana Reservior

Keywords: Plastic pollution; Shellfish; Aquatic environment; Bivalve

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT013

Dietary Fishmeal Replacement with Crickets and Bamboo Caterpillar Meal for Cobia (Rachycentron canadum)

Supamas Sriwongpuk1,*, Pitchaya Chainak2 and Krit Kawchada1

1Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Pathumthani 12130, Thailand 2Phangnga Coastat Fisheries Research and Devetopment Center, Phangnga 82120, Thailand

*Corresponding Author: [email protected]

The study on replacing fishmeal with crickets (Gryllus testaceus) and bamboo caterpillar or bamboo worms (Omphisa fuscidentalis) in cobia diet. The objective are to study raw materials that can be used as a protein source to replace protein from fishmeal which has a lot of decline in nature from over-fishing and to study the growth rate of cobia fed from 3 different diet formulas: treatment 1 (control) was diet pill trials (experimental pellet diet) with 70% fish meal protein, treatment 2 was experimental pellet diet with 40% fishmeal protein mixed with 30% protein from cricket and treatment 3 was experimentally fed pellets containing 40% fish meal mixed with 30% bamboo caterpillar protein. The cobia fish (Rachycentron canadum Linnaeus, 1766) used in this study had an average initial length of 23.3±0.4 cm and an average initial weight of 85.83±3.87 grams and were fed with the experimental food for 60 days. The results showed that the cobia fish fed with all 3 formulas were not significantly different (P>0.05) in the growth rate (both weight and average length from the beginning to the end of the experiment), the survival rate, the feed conversion ratio (FCR) and the feed intake. The results concluded that all three types of raw materials above can be used as a renewable source of protein from fish meal and also help to preserve fishery resources.

Keywords: Dietary fishmeal replacement; Cobia; Rachycentron canadum; Crickets; Bamboo caterpillar

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT014

Immune Response Analysis of Fairy Shrimp Branchinella thailandensis Against Bacterial Black Disease by de novo Transcriptome Analysis

Eakapol Wangkahart1,*, Wipavee Thaimuagphol1 and La-orsri Sanoamuang2

1Division of Fisheries, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand 2Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand

*Corresponding Author: [email protected]

To enhance genomic resources and understand the molecular immune mechanisms underlying the response topathogens, we first performed a comparative gene transcription analysis from Aeromonas hydrophila-immunized Streptocephalus sirindhornae and from a control group through RNA-Seq technology, meanwhile the differentially expressed genes (DEGs) were investigated. In total, 92,597,114 clean reads were obtained and then assembled into 73,297 unigenes with an average length of 993 bp and an N50 of 1458 bp. Unigenes were annotated by comparing against nr, Swiss-Prot\KEGG\ COG\ KOG\ GO and Pfam databases, and 37,334 unigenes (50.9%) were annotated in at least one database. After bacterial challenge, 143 and 287 genes were identified as remarkably up-regulated or down-regulated, respectively, amongst 856 were associated with immune system. This study characterized a gene expression pattern for normal and A. hydrophila-immunized S. sirindhornae for the first time and sheds new light on the molecular mechanisms thus enabling future efforts on disease control programs in this valuable aquaculture species.

Keywords: Branchinella thailandensis; Streptocephalus sirindhornae; Gene expression; Transcriptome analysis; Immune response

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT015

Using the K-Nn Weather Generator Algorithms in Predicting Evaporation for Reference Crop Evapotranspiration

Surasit Punyawansiri* and Bancha Kwanyuen

Department of Irrigation Engineering, Faculty of Engineering, Kasetsart University, Nakhon Pathom, 73140, Thailand

*Corresponding Author: [email protected]

Evaporation is important for water planning, hydrological and crop water requirements. This study demonstrates the application of the K-Nearest Neighbor (K- NN) weather generator algorithms approach for predicting evaporation (mm). The approach was assessed using data series of evaporation of Phitsanulok Meteorological Station (WMO Index 48378) for 1962 to 2019 (the baseline period) as well as for 2021- 2040 (near future), 2041–2060 (medium future) and 2061–2080 (far future). Various statistic measures Pearson’s correlation coefficient (r), Mean Absolute Error (MAE), the Nash-Sutcliffe coefficient of efficiency (NSE) and Willmott’s index of agreement (d) were deployed to evaluate and compare the performance of the models during the baseline period is very good accurate. It was also found that the future change of evaporation tends to be higher. Moreover, the future local surface evaporation in 2021- 2040, 2041-2060 and 2061-2080 tends to be increasing than 1962-2019, were 1.74, 2.37, 2.55 (%), respectively .Therefore, the K-NN weather generator algorithms has reasonable skill to simulate for evaporation and can be adopted as an effective tool for incorporating climate change impacts in ecology, hydrology and agriculture.

Keywords: K-Nearest Neighbor; Evaporation; Weather generator; Algorithm

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT016

Growth of Caesalpinia sappan L. by Using Different Growing Media and Evaluation of Antioxidant Activity of Foliage

Supreena Srisaikham and Quanjai Rupitak*

Faculty of Agricultural Technology, Burapha University, Sa Kaeo Campus, Sa Kaeo 27160, Thailand

*Corresponding Author: [email protected]

Caesalpinia sappan L. is a herb and dyeing plant which is found naturally in Thailand. Cultivation is an alternative way to conserve C. sappan in nature. Farmers in local areas are interested in planting C. sappan because of its agricultural by-products. This study investigated the effects on C. sappan L. of rice husk, rice husk biochar, coconut coir dust and their combination as growing media on growth, TPC, TFC and antioxidant activity. The results showed that plant height, stem diameter, leaf length, leaf width, petiole length and leaf number increased from 1 to 8 months after transplanting to a field. All treatments produced greater plant height, stem diameter and leaf numbers after 8 months following transplanting, except for the coconut coir dust treatment (T4). In addition, there was a positive correlation between plant height and stem diameter. After three and a half years, stem diameter and the total flavonoid compound of C. sappan grown in rice husk (T2) were higher than control. At this period, there was no correlation between stem diameter and TPC, TFC or antioxidant activity. All the treatments of C. sappan produced diameters ranging from 49.2 to 69.9 mm. In conclusion, the growing media after composting had a greater effect on plant growth than non-composting. The identification of bioactive compounds and antioxidant activity from C. sappan for all treatments may play an important role in health benefits and for developing novel functional ingredients in animal feed.

Keywords: Antioxidant activity; Rice husk; Rice husk biochar; Coconut coir dust; Caesalpinia sappan

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT017

Effects of Probiotics on Survival, Blood Chemical of Nile Tilapia under Transportation Stress

Nantaporn Sutthi*

Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding Author: [email protected]

The effect of probiotic treatment in water on the control group (no probiotic); T1, 5.6x108 cfu g-1 of Saccharomyces cerevisiae; T2, and 1x106 cfu g-1 of Bacillus spp.; T3 was evaluated for survival and blood chemical in Nile tilapia (Oreochromis niloticus) under transportation stress. After Nile tilapia reared in aqueous probiotic for 120 days were used to test transportation stress for 4 h. There were no significant differences in survival rate during transportation and after transportation for all treatments. However, survival rate of the control group was significant decreased (p<0.05) after 24 h of transportation stress. Blood chemical profile showed significant differences for cortisol, glucose, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity, with T1 group higher than T2 and T3 groups. Moreover, T3 group showed lower malondialdehyde (MDA) than those of T1 and T2 groups at 24-72 h after transportation. We concluded that Nile tilapia reared for 120 days in aqueous probiotic showed improved health tolerance under transportation stress.

Keywords: Nile tilapia; Probiotic; Transportation stress; Survival rate

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT018

A Survey of Cattle Rumen Fluke at Butcher Shops in Maha Sarakham Province, Thailand

Kedsirin Sakwiwatkul1,*, Chakrapong Chaikong1, Chanyuth Thamwan1, Siripan Ponsan1, Dumrongrak Rakwongrit2, Julawut Kudhinnog1, Jiraporn Jugdee1 and Benchapan Intarikanon1

1Animal Sciences, Faculty of Technology, Mahasarakham University, Kantarawichai District, Maha Sarakham 44150, Thailand. 2Agricultural Technical officer, Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.

*Corresponding Author: [email protected]

Types and prevalence infection of rumen flukes were investigated in 213 slaughtered cattle from 7 butchers in Maha Sarakham province. The result found that beef was infected the parasites more than dairy (99.53% vs 0.47%). The females were found the parasites more than males (83.10% vs 16.90%). Non pregnant cattle were infected parasites more than pregnant cattle (97.65% vs 2.35%). The most prevalence of rumen fluke was in Bandindum butchers (64.52%) compared to Chaingyuen, Borabue, Kantharawichai and Wapipathum Districts. Calicopholon spp. was mostly found (96.49%) in infected cattle. The prevalence of rumen fluke in cattle in this area were associated with sex and type of the animal. However, age did not effect to the prevalence of the parasite. The present study demonstrated that gastrointestinal infection occurs frequently in cattle around Mahasarakham province, especially rumen flukes infections. The results indicated that a management system should be used for the control of parasitic infections in cattle farms in Mahasarakham province and slaughterhouse.

Keywords: Rumen fluke; Butcher shops; Maha Sarakham province

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT019

Utilzation of Melon Husk on Productive Performance, Egg Quality and Bone Quality of Laying Hens

Tawat Chinrasri*, Kedsirin Sakwiwatkul, Ariya Jandawong, Kanthima Chaiyaphod and Warinyupa Yasakha

Animal Sciences, Faculty of Technology, Mahasarakham University, Kantarawichai District, Maha Sarakham 44150, Thailand.

*Corresponding Author: [email protected]

Objectives of this study were to determine the effect of utilization of a dried melon husk on productive performance, egg quality and bone quality of laying hens. Using Completely Randomized Design (CRD) a total of 80, eighteen-week old, Rhode Island Red laying hens were separated into 4 treatments of 4 replicates and each replicates had 5 layer hens. The dietary treatments were T1: control diet, T2, T3 and T4: control diet plus 2.5%, 5% and 10% of melon husk respectively. The results indicated effect of using melon husk at level 2.5% resulted in improved feed intake in laying hen, but feed conversion ratio of hen decreased when compared with control group. Using melon husk at level 2.5% resulted in improved egg weight and egg yolk color when compared with control group. Effect of using melon husk at level 2.5% resulted in tibial bone length increased. But of tibial bone weight, tibial bone thickness and tibial bone ash did not difference This study concluded that melon husk improved egg yolk color by feeding, so melon husk can be used more efficiently for hens egg yolk pigmentation for consumers prefers the darkest yolk colors.

Keywords: Melon; Hen egg; Egg quality; Tibia; Laying hen

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

PAT020

Antimicrobial Activity of Some Herbal Plant Extraction and Biological Fermented Products from Nasinuan Sub-District, Maha Sarakham, Thailand

Panarat Phadee1,*, Waranyoo Kaewduangta1, Eakapol Wangkahart1 and Issaraporn Somboonwattanakul2

1Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Katarawichai, Maha Sarakham 40150, Thailand 2Department of Biotechnology, Faculty of Technology, Mahasarakham University, Katarawichai, Maha Sarakham 40150, Thailand

*Corresponding Author: [email protected]

Studies on the capacity of some herbal plants in Nasinuan Sub-district on antimicrobial activity of aquatic animal diseases. Green pome tree, Indian oak and Indian almond leaves were extracted using ethanol and methanol, in addition, the phytochemical screening of 10 components. The 18 bio-fermented herbal plant formula using EM and Bacillus subtilis were also carried out. Moreover, the MIC and MBC of the 3 herbal sets to the 4 strains of the genus Streptococcus and Aeromonas were examined. There was found that the methanol extracted herbal plants showed higher yield than ethanol extraction and the phytochemical of Green pome tree consists of Coumarin, Tannins, Steroids, Flavonoids and Saponins, respectively, phytochemical of Indian oak consists of Saponins, Terpenoid, Flavonoids, Cardiac glycosides, Tannins and Steroids, respectively, and phytochemical of Indian almond consists of Saponins, Terpenoid, Tannins, Flavonoids, Coumarin and Cardiac glycosides, respectively. The 18 Bio-fermented juice of 3 herbal plants showed pH 2.79-3.84 at week 8, and in 1st week found 102-104 CFU/ml microorganisms in the juices, while after 3-4 weeks could not found microorganisms in the juices. The IMC and MBC of Green pome tree, Indian oak and Indian almond extracted found that the methanol extracted showed high efficiency to bacteria than ethanol. This was showed that MIC and MBC of herbs were in the range of 650-2,500 and 312.5-2,500 ppm to Aeromonas spp. and Streptococcus spp., respectively. There was revealed that Indian almond extracted using methanol was the most effective, followed by methanol extracted Green pome tree and Indian oak, respectively. Whereas, the IMC and MBC of bio-fermented juice found that the formula 8 was the most effective, followed by formula 9, 3, 15, 12 and 7, respectively.

Keywords: Herbal plant extraction; Fermented herbal plant; Green pome tree; Indian oak; Indian almond

ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

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ICoFAB2020 International Conference on Food, Agriculture and Biotechnology

Note