Study on inheritance of microsatellite markers in hybrid of chalcaburnus chalcoides and vimba persa in southern part of

Item Type monograph

Authors Rezvani Gilcolaei, Sohrab; Rouholahi, Sh.; Shojaie, H.; Laloei, F.; Taghavi, M.J.; Nayerani, M.

Publisher Iranian Fisheries Science Research Institute

Download date 06/10/2021 04:26:44

Link to Item http://hdl.handle.net/1834/39653 (Chalcalburnus Chalcoides) ( persa) (Chalcalburnus Chalcoides) (Vimba vimba persa)

(Vimba vimba persa) (Chalcalburnus Chalcoides)

DNA

DNA

PCR

PCR

PCR

LCO3 LCO1 Rru2 Lidll Z7-8 Z1-2 Z9-10

Ne Na

Rst Fst

Chalcalburnus chalcoides

Vimba vimba persa

DNA

DNA

PCR

UVI DOC Version V.99.4 PCR

Chi-square F1

AMOVA RST FST

TFPGA Gene Alex

LCO3 ,

P<0.05 Rru2 LCO1 ,Lid11

Z9-10 Z7-8 Z1-2

Chalcalburnus Vimba vimba persa

chalcoides

Berg, 1949

Krasznai, 1987

Coad, 1995

DNA

DNA RFLP

Elo et al, 1997

Sugaya et al, 2002;

Gharibkhani et al, 2009

Peng et al, 2005

Aladin and

Plotnikov, 2004

Gobidae Clupeidae

Coad, 1995

1 fractional

Mesopotamia

Coad 1995

(Winfield and Nelson, 1991)

Phylum: Chordata Subphylum: Vertebrata

Class: Osteichthyes Subclass:

Order: Sub order: Cyprinidei

Family:Cyprinidae Subfamily: Alburninae

Genus: Chalcalburnus Species: chalcoides (Güldenst?dt, 1772)

Yildirm et al, 2003 Chalcalburnus belvica Chalcalburnus mossulensis Chalcalburnus tarichi Chalcalburnus sellal Chalcalburnus chalcoides

Chalcalburnus chalcoides

sepid kooli kas kooli shah kooli

Seelaube aslak

Coad, 1999 Shemaya

Euphrates Tigris

IUCN

Kiabi et al, 1999; Dikov and Zivkov, 2004

Olah Holcik

Coad, 1995

Coad, 1995

2 International Union for Conservation of Natural Resources (Winfield and Nelson, 1991) Phylum: Chordata Subphylum: Vertebrata

Class: Osteichthyes Subclass: Actinopterygii

Order: Cypriniformes Sub order: Cyprinidei

Family:Cyprinidae Subfamily:

Genus: Vimba Species: vimba Subspecies: persa (Pallas, 1814)

Vimba vimba vimb

Vimba vimba tenella

Vimba vimba persa

Vimba vimba persa

Cooli Kooli

Caspian vimba Kaspiiski Chemospinka Garasol

Coad, 1999

IUCN

Kiabi et al, 1999

zebra

Stelkens et al, 2008

niche

. Beach et al, 2006

Beach et al, 2006

Campton, 1987

Poly, 1997

(Unver et al., 2005) Chalcalburnus Leuciscus

Coad, 1995

Poly, 1997

Johann Gregor Mendel

Pisum sativum

3 Menedian Inheritance

I

http://en.wikipedia.org/wiki/mendelian_inheritance

4 Dominant allele 5 Recessive allele 6 Low of segregration 7 Low of Independent assortment

Caralli-Sforza and Edwards, 1967; Rezvani et al, 2006

NJ

Tamura et al, 2007

UPGMA

Tamura et al, 2007

Nyx Nxx N

8 Neighbor-joining method 9 Unweighted Pair-Group Method Using an Arithmetic Avreage

Chi-square

E O

FST,RST

FST

RST

10 Hardy Weinberg Equlibrium

FST

He HT

AMOVA FST

VWP VAR VAP

Beardmore et al, 1997

11 Gene or Hetrozygosity Variation

p He

N Ho

Na

Ne

P

y x Jy ,Jx ,Jxy Ne

12 Alleles Number 13 Effective Alleles 14 Genetic distance and Genetic identity

FST Nm

15 Gene flow

DNA

Okumus and Ciftic, 2003

Chistiakov et al, 2006

DNA

Okumus and Ciftci, 2003 nDNA DNA

DNA

DNA

AC n

Chistiakov et al , 2006 DNA

SSLPs SSR

19 SSMs STMs

mtDNA Toth et al, 2000 STMs

kbp

kbp

Wright, 1994

16 Simple sequence repeat 17 Simple sequence length polymorphisms 18 Short tandem repeats motifs 19 Simple sequence 20 Sequence tagged microsatellite sites 21 Kilo base pair

PCR DNA

QTL

Wright and

Bentzen, 1994

DNA

bp 24PCR

Stutter

(O'Reilly et al, 1998 PCR

22 Quantitative Trait Loci 23 Null alleles 24 Polymerase chain reaction

RAPD Jamshidi

Hypophthalmichthys nobilis Ctenopharyngodon idella

F1 RAPD

Poecilia reticulata RAPD Foo

F1

F1 RAPD

Banks 27

Oncorhynchus tschawytscha Chinook Novel

DNA Sugaya

Penaeus japanicus kumura

DNA

Thunnus DNA Takagi

albacares

25 Random Amplified Polymorphic 26 Fingerprints 27 Genetic linkage mapping

Wang Fenneropenaeus chinesis EST-SSR

F.chinesis

Peng

Mystus nemurus

F1

Zhan Argopecten irradians

Crassostrea Li

gigas

Paralabrax kelp bass Vogel

clathratus

Oreochromis DNA Rashed

niloticus Coad, 1995

ovaprim

F1

DNA

DNA DNA

SDS STE

K

K

DNA

DNA

DNA

DNA

.

DNA

Samboork and Russell, 2006

DNA

TBE

28 Sampler

TBE

DNA

Samboork and DNA DNA

Russell, 2006

Shimoda et al, 1999;

Hamilton and Tyler, 2007 L L L L R S L C C C Y i r i d u d O O O P 1 - 1 2 5 1 5 1 3 R R F F : - : C - A A R F C A R F R R T F G - - : A F A : F A : : G T : T G C : R G : C T A T T C G C T A T : G A G T A A A G T T T G T A A A A G T G C C G C C C G T G A G G C C G A A A A G T T A A T T A C G C G C A G A A G G C G A G C G T T A C G A G G A A G C A T A G C C A C T C T T T T T T C C A A A T G T G A C T C G A A A C T T T A C T C G C T A T T C C T T A T T A G G T A A A T G C A G G A G C T A A G A T A A C A T G T T T G G C C G G A G C C G A G T T G A A G A C G T A A T C G A T C C C A A G T G T C T A T A A A G T G A G T A A G C C A G A A A T A A A A T A G T T G A G G T A A C T G T A T G A T A T G T G A T G T C C G C T G G T A A A A T T C G [ T G T [ [ C ( ( ( C [ G G T C C T A ] A A T 6 T T G G [ [ T [ ) ) ) ( T G T 9 A 7 5 A A A ( ( A G A ] C C ) ] 2 ( T T 8 ] [ T T A A G 9 C C C A G ) ) G A ] 1 2 ] ] 1 ) 3 2 0 0 C ] 6 5 T 1 4 A T A A A A A A A A Y Y Y Y B B B 1 1 1 3 3 3 3 1 1 1 1 1 1 1 2 2 2 8 8 8 8 7 7 7 7 7 7 7 3 3 3 8 7 7 7 8 6 2 1 8 7 9

Z Z Z 9 7 1 - - - 1 8 2 0 R R F F R - - - - F G A - A A - C C T G G T T G G T G C C G G T A T G C A A A G G T C T C T G C T C T C G A A G T A C C C G A T A A A G C A G G T T G A G C T C C C A G A C T C G T A T G C G T G A G T G C G T G T C A T G A C C G T A A A C G G G T C G C T T ( ( ( S S S h h h i i i m m m 1 1 1 o o o 9 9 9 d d d 9 9 9 a a a 9 9 9 ) ) ) e e e t t t a a a l l l . . . , , ,

DNA

MgCl2 PCR Taq polymeras dNTP

Fei et al, 2007

PCR

PCR PCR

PCR dNTPs MgCl2 DNA

Shafiqur et al, 2008

PCR

(min)

. . .

PCR http://www.mun.ca/biology/scarr/PCR_simplified.html

PCR

PCR

MBI Fermentas pBR322 DNA/AluI Marker, 20bp DNA PCR

Rodzen and May, 2002 PCR

TBE (10X)

TEMED

PCR TBE (1X)

TBE (1X)

DNA

DNA

29 spacer

. A

. B

.

NaoH . NaBH4 . C

NaOH

. NaBH4

.

C

A

B

C

DNA

DNA PCR pBR322 DNA/AluI

Rezvani, 1997 Marker, 20,

UVI DOC Version V.99.04 UVI DOC0080 XD

PCR

PCR

UVI DOC Version V.99.04

Peng et al, 2005 Chi-square

(UPGMA)

31 TFPGA version 1.3

Salari et al, 2008 Nei (1978)

30 Tools for Population Genetic Analyses 31 10000 permutations conducted 32 Molecular Evolutionary Genetics Analysis

version 4 NJ

(Tamura et al., 2007) (MEGA) software version 4.0 MEGA

Nei (1972, 1978)

33 AMOVA FST RST X

Peakwall and Smouse, 2005 Gene Alex version .

33 Analysis of Molecular Variance

. . .

. . .

DNA

DNA

DNA DNA

PCR

DNA

PCR

PCR pBR322 DNA/AluI Marker, 20 PCR

LCO3

PCR

LCO3

LCO3

LCO1

PCR

LCO1

200 LCO1

Rru2

PCR

Rru2

Rru2

Lid11

PCR

Lid11

Lid11 100

Z7-8

PCR

Z7-8

Z7-8

Z1-2

PCR

Z1-2

Z1-2

Z9-10

PCR

Z9-10

Z9-10

DNA

LCO3 Z7-8

LCO1 LCO3 Rru2 Lid11 Z7-8 Z9-10 Z1-2

LCO3 LCO1

Chi-square Rru2 Lid11

P 0.05

Z9-10 Z7-8 Z1-2

Z1-2 Z9-10 Z7-8 Z1-2

Z9-10 Z7-8 F1

LCO1 LCO3

Lid11 Rru2 Z9-10 Z7-8 Z1-2

Z9-10 Z7-8 Z1-2

P 0.05 F1 P 1 F 1 1 F F

LCO3

LCO1

Lid11

Rru2

Z1-2

Z7-8

Z9-10

LCO3

LCO3 F1

LCO1

LCO1 F1

Rru2

Rru2 F1

Lid11

Lid11 F1

Z9-10

Z9-10 F1

Z1-2

Z1-2 F1

Z7-8

Z7-8 F1

Ne Na

Z7-8

LCO3 LCO1

Z7-8

LCO3

n =10 n =10 n=10 Na Ne Na Ne Na Ne . . . Z1-2

. . . Z9-10

. . . Z7-8

. . . Lid11

. . . Rru2

. . . LCO3

. . . LCO1

......

(He) (Ho) Ho . . Lid11 LCO1 LCO3 Z7-8 Z9-10 Z1-2 LCO3 Rru2 . . He Z7-8 . LCO3 .

He Ho

He Ho

n =10 n =10 n=10

Ho He Ho He Ho He . . . . . Z1-2 . . . . . Z9-10 . . . . . Z7-8 . . . . Lid11 . . . . . Rru2 . . . . . LCO3 . . . LCO1 ......

P< P< Rru2 Lid11

LCO1 LCO3 Rru2 Lid11 Z7-8 Z9-10 Z1-2

ns Ns ** Ns Ns ns ns

ns Ns Ns * Ns ns ns

ns Ns Ns ns Ns ns ns Key: ns=not significant , * P<0.05 , ** P<0.01 , *** P<0.001

Rst Fst

Rst Fst

Fst

Nm Rst Fst

Nm

Nm Rst Fst

Fst

. * . * .

. . * .

. . .

(P<0.01) *

Rst

. . .

. . .

. . .

Nm)

Nm .

.

.

GeneAlex 1972 Nei (1972) Nei 0.710 0.851

(Nei, 1972)

. . .

. . .

. . .

(1978) Nei

MEGA NJ UPGMA

TFPGA

( Nei, 1978) UPGMA c a b a MEGAversion 4.0

g f b e d UPGMA(Nei, 1978) hybrid siyah shah

MEGAversion 4.0 ( Nei, 1978) NJ

b2 b1 b a2 a1 a b a

b 2

b

b 1

a 1

a 2

a NJ(Nei,1978) hybrid siyah shah

TFPGA ( Nei, 1978) UPGMA

b2 b1 b version 1.3

( Nei, 1978) UPGMA

0.525 0.350 0.175 0.700 0.000 b 1 b 2 b

a

DNA

RFLP

Elo et al, 1997 DNA

DNA

DNA PCR

DNA

Elo et al, 1997

RAPD AFLP

Backcross

Peng et al, 2005

34 Interspecific hybridization 35 Population subdivision

Huvet et al, 2001; Selvamani et al, 2001; Xu et al, 2001; Rodzen and May, 2005

(Vimba vimba persa,

Chacalburnus chalcoides

Peng

2005

2009 Li Mystus nemurus

2009 Rashed Crassostrea gigas Pacific oyster

Oreochromis

36 GenBank

Mystus nemurus Peng et al, 2005 Oreochromis niloticus Rashed et al, 2009 Crassotrea gigas Li et al, 2009 Chalcalburnus chalcoides Vimba vimba persa

. . 2000 Dewoody and Avise (Dewoody and Avise, 2000

.

. . .

.

2008 Salari

. .

. . Dewoody &Advise et al.,2000 . Rochycentron canadum . Salari et al, 2008 Vimba vimba persa . Chalcalburnus chalcoides . Vimba vimba persa . .

H

Fei et

al, 2007

Dewoody and Avise, 2000

. . Ho

. .

Dewoody and Avis, 2000 Ho= .

. . He

Z7-8

LCO3

Fei Beadmore et al, 1997

Cirrohina moliterolla Mud carp DNA 2007

. . He . . Ho

Kavan Cirrohina moliterolla

2009

.

Dewoody and Avis, 2000

Ho . . Dewoody &Advise et al.,2000 . Cirrohina moliterolla . . Fei et al, 2007 kutum . Kavan et al, 2009 Rochycentron canadum . Salari et al, 2008 Acipenser gueldenstaedtii . . Chalcalburnus chalcoides . Vimba vimba persa . .

Fei

2008 Salari

Pikeperch 2009 Gharibkhani

Acipenser gueldenstaedtii

Poteaux Salmo trutta Mediteranean brown trout

1999

Salmo trutta Poteaux et al, 1999 Cirrohina moliterolla Fei et al, 2007 Rochycentron canadum Salari et al, 2008 Sander luciperca Gharibkhani et al, 2009 Acipenser gueldenstaedtii Vimba Chalcalburnus chalcoides vimba persa

Hou, 2007

DNA

2005 Lundrigan

UPGMA

Fst

Rachycentron canadum

Imsiridou

RFLP Leuciscus cephalus 1998

Fst

Fst

Fst

Fst Tevfic Dorak, 2010

2009 Rashed

RAPD

F1

Fst RAPD F1

Fst

(Nm) Fst

Nm<

Beacham et al, 2000 Nm<

P< Fst

Nm< Nm

Fst

Fst Oreochromis niloticus Rashed et al, 2009 Vimba vimba persa Vimba Chalcalburnus chalcoides vimba persa

Lid11 Rru2 LCO1 LCO3

2003 Takagi P 0.5

Thunnus albacares DNA

DNA 2002 Sugaya

2009 Li Penaeus japanicus

Pacific oyster

2009 Vogel Kelp bass

RAPD 2009 Jamshidi

RAPD

F1

Penaeus japanicus Sugaya et al, 2002 Thunnus albacares Takagi et al, 2003 Crassotrea gigas Li et al, 2009 Paralabrax clathratus Vogel et al, 2009 grass carp and bighead carp Jamshidi et al, 2009 Vimba Chalcalburnus chalcoides vimba persa

Z9-10 Z7-8 Z1-2

Zhan et al, 2007; Peng et al, 2005; Corte-Real et al, 1994

Zhan

Sugaya Argopecten irradians bay scallop EST-SSR

2002

2005 Peng

DNA 1994 Corte-Real

Corte-Real

2002 Sugaya 2005 Peng

Chinook Novel 1999 Banks

Oncorhynchus tschawytscha salmon

38

virus-mediated

2007 Zhan

2002 Sugaya

Zhan

Sugaya et al, 2002

37 meiotic drive 38 prezygotic 39 gene conversion 40 postzygotic event 41 Differential viability 42 Differential functionality of gamete

PCR priming site

Banks et al, 1999

bivalve larvae Corte-Real et al, 1994

Oncorhynchus tschawytscha Banks et al, 1999

Penaeus japanicus Sugaya et al, 2002

Mystus nemurus Peng et al, 2005

Argopecten irradians Zhan et al, 2007

Vimba Chalcalburnus chalcoides vimba persa

QTL

bottle nock

PCR

Acipenser gueldenstaedtii

PCR-RFLPs

Rachycentron

canadum

. . . . .

. .

.

Barbus

PCR-RFLP capito

Vimba vimba persa

Vimba vimba persa

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Abstract: In order to study the Inheritance of Microsatellite Markers in the hybrid of Chalcalburnus chalcoides and Vimba vimba persa in the Southern part of the Caspian Sea, hybridization between the species was carried out during spring (2009) in Shahid Ansari reproduction and culture workstation. 30 samples from the produced larva and also small piece of dorsal and pectoral fin of their parents were collected and stored in pure ethanol (96%) and transferred to the biotechnology of the Caspian Ecology Academy. DNA was extracted from fin samples of the parents, whilst the whole F1 progeny was used to isolate the DNA by Phenol-chloroform method. Quantity and quality of extracted DNA was determined using 1% Agarose electrophorus. DNA was used for PCR ampilification of 10 microsatellite loci. The amplifications were run on 6% Polyacrylamid electrophorus. The present study showed that 7 out of 10 markers were polymorphic and variated between the parents. Goodness of fit to the Mendelian inheritance ratio was determined by X2 analysis. 4 primers were segregrated according to Mendelian expectations at a significance level of P 0.05. Based on UPGMA dendrogram (Nei, 1978) two different clusters were obtained in which Chalcalburnus chalcoides and hybrid were in the same cluster but Vimba vimba persa was in the other. It seems that Vimba vimba persa is a joint ancestor for these two. High heterozygosity presence in parents and their F1 shows the high potential of genetic variety in Chalcalburnus chalcoides and Vimba vimba persa which from the aquaculture management and conservation view is important and considering the high genetic variety in their hybrid, the hybrid has the sufficient potential for race improvement. Key word: Chalcalburnus chalcoides, Vimba vimba persa, hybrid. Microsatellite, Caspian Sea

Ministry of Jihad e Agriculture AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION Caspian Sea Ecology Research Center Inland Waters Aquaculture Research Center

Title : Study on inheritance of microsatellite markers in hybrid of chalcaburnus chalcoides and vimba vimba persa in southern part of Caspian Sea Apprpved Number: 4-12-12-89030 Author: Sohrab Rezvani Gilcolaei Executor : Sohrab Rezvani Gilcolaei Collaborator : Sh. Rouholahi, H. Shojaie, F. Laloei, M.J.Taghavi, M. Nayerani Advisor(s): A.Salari Location of execution : Guilan & Mazandarn provinces Date of Beginning : 2010 Period of execution : 1Year Publisher : Iranian Fisheries Research Organization Circulation : 20 Date of publishing : 2011 All Right Reserved . No Part of this Publication May be Reproduced or Transmitted without indicating the Original Reference

MINISTRY OF JIHAD - E - AGRICULTURE AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION- Caspian Sea Ecology Research Center Inland Waters Aquaculture Research Center

Title: Study on inheritance of microsatellite markers in hybrid of chalcaburnus chalcoides and vimba vimba persa in southern part of Caspian Sea

Executor : Sohrab Rezvani Gilcolaei

Registration Number 2010.1132