Study on inheritance of microsatellite markers in hybrid of chalcaburnus chalcoides and vimba vimba persa in southern part of Caspian Sea
Item Type monograph
Authors Rezvani Gilcolaei, Sohrab; Rouholahi, Sh.; Shojaie, H.; Laloei, F.; Taghavi, M.J.; Nayerani, M.
Publisher Iranian Fisheries Science Research Institute
Download date 06/10/2021 04:26:44
Link to Item http://hdl.handle.net/1834/39653 (Chalcalburnus Chalcoides) (Vimba vimba persa) (Chalcalburnus Chalcoides) (Vimba vimba persa)
(Vimba vimba persa) (Chalcalburnus Chalcoides)
DNA
DNA
PCR
PCR
PCR
LCO3 LCO1 Rru2 Lidll Z7-8 Z1-2 Z9-10
Ne Na
Rst Fst
Chalcalburnus chalcoides
Vimba vimba persa
DNA
DNA
PCR
UVI DOC Version V.99.4 PCR
Chi-square F1
AMOVA RST FST
TFPGA Gene Alex
LCO3 ,
P<0.05 Rru2 LCO1 ,Lid11
Z9-10 Z7-8 Z1-2
Chalcalburnus Vimba vimba persa
chalcoides
Berg, 1949
Krasznai, 1987
Coad, 1995
DNA
DNA RFLP
Elo et al, 1997
Sugaya et al, 2002;
Gharibkhani et al, 2009
Peng et al, 2005
Aladin and
Plotnikov, 2004
Gobidae Cyprinidae Clupeidae
Coad, 1995
1 fractional
Mesopotamia
Coad 1995
(Winfield and Nelson, 1991)
Phylum: Chordata Subphylum: Vertebrata
Class: Osteichthyes Subclass: Actinopterygii
Order: Cypriniformes Sub order: Cyprinidei
Family:Cyprinidae Subfamily: Alburninae
Genus: Chalcalburnus Species: chalcoides (Güldenst?dt, 1772)
Yildirm et al, 2003 Chalcalburnus belvica Chalcalburnus mossulensis Chalcalburnus tarichi Chalcalburnus sellal Chalcalburnus chalcoides
Chalcalburnus chalcoides
sepid kooli kas kooli shah kooli
Seelaube aslak
Coad, 1999 Shemaya
Euphrates Tigris
IUCN
Kiabi et al, 1999; Dikov and Zivkov, 2004
Olah Holcik
Coad, 1995
Coad, 1995
2 International Union for Conservation of Natural Resources (Winfield and Nelson, 1991) Phylum: Chordata Subphylum: Vertebrata
Class: Osteichthyes Subclass: Actinopterygii
Order: Cypriniformes Sub order: Cyprinidei
Family:Cyprinidae Subfamily: Leuciscinae
Genus: Vimba Species: vimba Subspecies: persa (Pallas, 1814)
Vimba vimba vimb
Vimba vimba tenella
Vimba vimba persa
Vimba vimba persa
Cooli Kooli
Caspian vimba Kaspiiski Chemospinka Garasol
Coad, 1999
IUCN
Kiabi et al, 1999
zebra
Stelkens et al, 2008
niche
. Beach et al, 2006
Beach et al, 2006
Campton, 1987
Poly, 1997
(Unver et al., 2005) Chalcalburnus Leuciscus
Coad, 1995
Poly, 1997
Johann Gregor Mendel
Pisum sativum
3 Menedian Inheritance
I
http://en.wikipedia.org/wiki/mendelian_inheritance
4 Dominant allele 5 Recessive allele 6 Low of segregration 7 Low of Independent assortment
Caralli-Sforza and Edwards, 1967; Rezvani et al, 2006
NJ
Tamura et al, 2007
UPGMA
Tamura et al, 2007
Nyx Nxx N
8 Neighbor-joining method 9 Unweighted Pair-Group Method Using an Arithmetic Avreage
Chi-square
E O
FST,RST
FST
RST
10 Hardy Weinberg Equlibrium
FST
He HT
AMOVA FST
VWP VAR VAP
Beardmore et al, 1997
11 Gene or Hetrozygosity Variation
p He
N Ho
Na
Ne
P
y x Jy ,Jx ,Jxy Ne
12 Alleles Number 13 Effective Alleles 14 Genetic distance and Genetic identity
FST Nm
15 Gene flow
DNA
Okumus and Ciftic, 2003
Chistiakov et al, 2006
DNA
Okumus and Ciftci, 2003 nDNA DNA
DNA
DNA
AC n
Chistiakov et al , 2006 DNA
SSLPs SSR
19 SSMs STMs
mtDNA Toth et al, 2000 STMs
kbp
kbp
Wright, 1994
16 Simple sequence repeat 17 Simple sequence length polymorphisms 18 Short tandem repeats motifs 19 Simple sequence 20 Sequence tagged microsatellite sites 21 Kilo base pair
PCR DNA
QTL
Wright and
Bentzen, 1994
DNA
bp 24PCR
Stutter
(O'Reilly et al, 1998 PCR
22 Quantitative Trait Loci 23 Null alleles 24 Polymerase chain reaction
RAPD Jamshidi
Hypophthalmichthys nobilis Ctenopharyngodon idella
F1 RAPD
Poecilia reticulata RAPD Foo
F1
F1 RAPD
Banks 27
Oncorhynchus tschawytscha Chinook Novel
DNA Sugaya
Penaeus japanicus kumura
DNA
Thunnus DNA Takagi
albacares
25 Random Amplified Polymorphic 26 Fingerprints 27 Genetic linkage mapping
Wang Fenneropenaeus chinesis EST-SSR
F.chinesis
Peng
Mystus nemurus
F1
Zhan Argopecten irradians
Crassostrea Li
gigas
Paralabrax kelp bass Vogel
clathratus
Oreochromis DNA Rashed
niloticus Coad, 1995
ovaprim
F1
DNA
DNA DNA
SDS STE
K
K
DNA
DNA
DNA
DNA
.
DNA
Samboork and Russell, 2006
DNA
TBE
28 Sampler
TBE
DNA
Samboork and DNA DNA
Russell, 2006
Shimoda et al, 1999;
Hamilton and Tyler, 2007 L L L L R S L C C C Y i r i d u d O O O P 1 - 1 2 5 1 5 1 3 R R F F : - : C - A A R F C A R F R R T F G - - : A F A : F A : : G T : T G C : R G : C T A T T C G C T A T : G A G T A A A G T T T G T A A A A G T G C C G C C C G T G A G G C C G A A A A G T T A A T T A C G C G C A G A A G G C G A G C G T T A C G A G G A A G C A T A G C C A C T C T T T T T T C C A A A T G T G A C T C G A A A C T T T A C T C G C T A T T C C T T A T T A G G T A A A T G C A G G A G C T A A G A T A A C A T G T T T G G C C G G A G C C G A G T T G A A G A C G T A A T C G A T C C C A A G T G T C T A T A A A G T G A G T A A G C C A G A A A T A A A A T A G T T G A G G T A A C T G T A T G A T A T G T G A T G T C C G C T G G T A A A A T T C G [ T G T [ [ C ( ( ( C [ G G T C C T A ] A A T 6 T T G G [ [ T [ ) ) ) ( T G T 9 A 7 5 A A A ( ( A G A ] C C ) ] 2 ( T T 8 ] [ T T A A G 9 C C C A G ) ) G A ] 1 2 ] ] 1 ) 3 2 0 0 C ] 6 5 T 1 4 A T A A A A A A A A Y Y Y Y B B B 1 1 1 3 3 3 3 1 1 1 1 1 1 1 2 2 2 8 8 8 8 7 7 7 7 7 7 7 3 3 3 8 7 7 7 8 6 2 1 8 7 9
Z Z Z 9 7 1 - - - 1 8 2 0 R R F F R - - - - F G A - A A - C C T G G T T G G T G C C G G T A T G C A A A G G T C T C T G C T C T C G A A G T A C C C G A T A A A G C A G G T T G A G C T C C C A G A C T C G T A T G C G T G A G T G C G T G T C A T G A C C G T A A A C G G G T C G C T T ( ( ( S S S h h h i i i m m m 1 1 1 o o o 9 9 9 d d d 9 9 9 a a a 9 9 9 ) ) ) e e e t t t a a a l l l . . . , , ,
DNA
MgCl2 PCR Taq polymeras dNTP
Fei et al, 2007
PCR
PCR PCR
PCR dNTPs MgCl2 DNA
Shafiqur et al, 2008
PCR
(min)
. . .
PCR http://www.mun.ca/biology/scarr/PCR_simplified.html
PCR
PCR
MBI Fermentas pBR322 DNA/AluI Marker, 20bp DNA PCR
Rodzen and May, 2002 PCR
TBE (10X)
TEMED
PCR TBE (1X)
TBE (1X)
DNA
DNA
29 spacer
. A
. B
.
NaoH . NaBH4 . C
NaOH
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.
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DNA
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Rezvani, 1997 Marker, 20,
UVI DOC Version V.99.04 UVI DOC0080 XD
PCR
PCR
UVI DOC Version V.99.04
Peng et al, 2005 Chi-square
(UPGMA)
31 TFPGA version 1.3
Salari et al, 2008 Nei (1978)
30 Tools for Population Genetic Analyses 31 10000 permutations conducted 32 Molecular Evolutionary Genetics Analysis
version 4 NJ
(Tamura et al., 2007) (MEGA) software version 4.0 MEGA
Nei (1972, 1978)
33 AMOVA FST RST X
Peakwall and Smouse, 2005 Gene Alex version .
33 Analysis of Molecular Variance
. . .
. . .
DNA
DNA
DNA DNA
PCR
DNA
PCR
PCR pBR322 DNA/AluI Marker, 20 PCR
LCO3
PCR
LCO3
LCO3
LCO1
PCR
LCO1
200 LCO1
Rru2
PCR
Rru2
Rru2
Lid11
PCR
Lid11
Lid11 100
Z7-8
PCR
Z7-8
Z7-8
Z1-2
PCR
Z1-2
Z1-2
Z9-10
PCR
Z9-10
Z9-10
DNA
LCO3 Z7-8
LCO1 LCO3 Rru2 Lid11 Z7-8 Z9-10 Z1-2
LCO3 LCO1
Chi-square Rru2 Lid11
P 0.05
Z9-10 Z7-8 Z1-2
Z1-2 Z9-10 Z7-8 Z1-2
Z9-10 Z7-8 F1
LCO1 LCO3
Lid11 Rru2 Z9-10 Z7-8 Z1-2
Z9-10 Z7-8 Z1-2
P 0.05 F1 P 1 F 1 1 F F
LCO3
LCO1
Lid11
Rru2
Z1-2
Z7-8
Z9-10
LCO3
LCO3 F1
LCO1
LCO1 F1
Rru2
Rru2 F1
Lid11
Lid11 F1
Z9-10
Z9-10 F1
Z1-2
Z1-2 F1
Z7-8
Z7-8 F1
Ne Na
Z7-8
LCO3 LCO1
Z7-8
LCO3
n =10 n =10 n=10 Na Ne Na Ne Na Ne . . . Z1-2
. . . Z9-10
. . . Z7-8
. . . Lid11
. . . Rru2
. . . LCO3
. . . LCO1
......
(He) (Ho) Ho . . Lid11 LCO1 LCO3 Z7-8 Z9-10 Z1-2 LCO3 Rru2 . . He Z7-8 . LCO3 .
He Ho
He Ho
n =10 n =10 n=10
Ho He Ho He Ho He . . . . . Z1-2 . . . . . Z9-10 . . . . . Z7-8 . . . . Lid11 . . . . . Rru2 . . . . . LCO3 . . . LCO1 ......
P< P< Rru2 Lid11
LCO1 LCO3 Rru2 Lid11 Z7-8 Z9-10 Z1-2
ns Ns ** Ns Ns ns ns
ns Ns Ns * Ns ns ns
ns Ns Ns ns Ns ns ns Key: ns=not significant , * P<0.05 , ** P<0.01 , *** P<0.001
Rst Fst
Rst Fst
Fst
Nm Rst Fst
Nm
Nm Rst Fst
Fst
. * . * .
. . * .
. . .
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Rst
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Nm .
.
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GeneAlex 1972 Nei (1972) Nei 0.710 0.851
(Nei, 1972)
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. . .
. . .
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MEGA NJ UPGMA
TFPGA
( Nei, 1978) UPGMA c a b a MEGAversion 4.0
g f b e d UPGMA(Nei, 1978) hybrid siyah shah
MEGAversion 4.0 ( Nei, 1978) NJ
b2 b1 b a2 a1 a b a
b 2
b
b 1
a 1
a 2
a NJ(Nei,1978) hybrid siyah shah
TFPGA ( Nei, 1978) UPGMA
b2 b1 b version 1.3
( Nei, 1978) UPGMA
0.525 0.350 0.175 0.700 0.000 b 1 b 2 b
a
DNA
RFLP
Elo et al, 1997 DNA
DNA
DNA PCR
DNA
Elo et al, 1997
RAPD AFLP
Backcross
Peng et al, 2005
34 Interspecific hybridization 35 Population subdivision
Huvet et al, 2001; Selvamani et al, 2001; Xu et al, 2001; Rodzen and May, 2005
(Vimba vimba persa,
Chacalburnus chalcoides
Peng
2005
2009 Li Mystus nemurus
2009 Rashed Crassostrea gigas Pacific oyster
Oreochromis
36 GenBank
Mystus nemurus Peng et al, 2005 Oreochromis niloticus Rashed et al, 2009 Crassotrea gigas Li et al, 2009 Chalcalburnus chalcoides Vimba vimba persa
. . 2000 Dewoody and Avise (Dewoody and Avise, 2000
.
. . .
.
2008 Salari
. .
. . Dewoody &Advise et al.,2000 . Rochycentron canadum . Salari et al, 2008 Vimba vimba persa . Chalcalburnus chalcoides . Vimba vimba persa . .
H
Fei et
al, 2007
Dewoody and Avise, 2000
. . Ho
. .
Dewoody and Avis, 2000 Ho= .
. . He
Z7-8
LCO3
Fei Beadmore et al, 1997
Cirrohina moliterolla Mud carp DNA 2007
. . He . . Ho
Kavan Cirrohina moliterolla
2009
.
Dewoody and Avis, 2000
Ho . . Dewoody &Advise et al.,2000 . Cirrohina moliterolla . . Fei et al, 2007 Rutilus frisii kutum . Kavan et al, 2009 Rochycentron canadum . Salari et al, 2008 Acipenser gueldenstaedtii . . Chalcalburnus chalcoides . Vimba vimba persa . .
Fei
2008 Salari
Pikeperch 2009 Gharibkhani
Acipenser gueldenstaedtii
Poteaux Salmo trutta Mediteranean brown trout
1999
Salmo trutta Poteaux et al, 1999 Cirrohina moliterolla Fei et al, 2007 Rochycentron canadum Salari et al, 2008 Sander luciperca Gharibkhani et al, 2009 Acipenser gueldenstaedtii Vimba Chalcalburnus chalcoides vimba persa
Hou, 2007
DNA
2005 Lundrigan
UPGMA
Fst
Rachycentron canadum
Imsiridou
RFLP Leuciscus cephalus 1998
Fst
Fst
Fst
Fst Tevfic Dorak, 2010
2009 Rashed
RAPD
F1
Fst RAPD F1
Fst
(Nm) Fst
Nm<
Beacham et al, 2000 Nm<
P< Fst
Nm< Nm
Fst
Fst Oreochromis niloticus Rashed et al, 2009 Vimba vimba persa Vimba Chalcalburnus chalcoides vimba persa
Lid11 Rru2 LCO1 LCO3
2003 Takagi P 0.5
Thunnus albacares DNA
DNA 2002 Sugaya
2009 Li Penaeus japanicus
Pacific oyster
2009 Vogel Kelp bass
RAPD 2009 Jamshidi
RAPD
F1
Penaeus japanicus Sugaya et al, 2002 Thunnus albacares Takagi et al, 2003 Crassotrea gigas Li et al, 2009 Paralabrax clathratus Vogel et al, 2009 grass carp and bighead carp Jamshidi et al, 2009 Vimba Chalcalburnus chalcoides vimba persa
Z9-10 Z7-8 Z1-2
Zhan et al, 2007; Peng et al, 2005; Corte-Real et al, 1994
Zhan
Sugaya Argopecten irradians bay scallop EST-SSR
2002
2005 Peng
DNA 1994 Corte-Real
Corte-Real
2002 Sugaya 2005 Peng
Chinook Novel 1999 Banks
Oncorhynchus tschawytscha salmon
38
virus-mediated
2007 Zhan
2002 Sugaya
Zhan
Sugaya et al, 2002
37 meiotic drive 38 prezygotic 39 gene conversion 40 postzygotic event 41 Differential viability 42 Differential functionality of gamete
PCR priming site
Banks et al, 1999
bivalve larvae Corte-Real et al, 1994
Oncorhynchus tschawytscha Banks et al, 1999
Penaeus japanicus Sugaya et al, 2002
Mystus nemurus Peng et al, 2005
Argopecten irradians Zhan et al, 2007
Vimba Chalcalburnus chalcoides vimba persa
QTL
bottle nock
PCR
Acipenser gueldenstaedtii
PCR-RFLPs
Rachycentron
canadum
. . . . .
. .
.
Barbus
PCR-RFLP capito
Vimba vimba persa
Vimba vimba persa
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Abstract: In order to study the Inheritance of Microsatellite Markers in the hybrid of Chalcalburnus chalcoides and Vimba vimba persa in the Southern part of the Caspian Sea, hybridization between the species was carried out during spring (2009) in Shahid Ansari reproduction and culture workstation. 30 samples from the produced larva and also small piece of dorsal and pectoral fin of their parents were collected and stored in pure ethanol (96%) and transferred to the biotechnology of the Caspian Ecology Academy. DNA was extracted from fin samples of the parents, whilst the whole F1 progeny was used to isolate the DNA by Phenol-chloroform method. Quantity and quality of extracted DNA was determined using 1% Agarose electrophorus. DNA was used for PCR ampilification of 10 microsatellite loci. The amplifications were run on 6% Polyacrylamid electrophorus. The present study showed that 7 out of 10 markers were polymorphic and variated between the parents. Goodness of fit to the Mendelian inheritance ratio was determined by X2 analysis. 4 primers were segregrated according to Mendelian expectations at a significance level of P 0.05. Based on UPGMA dendrogram (Nei, 1978) two different clusters were obtained in which Chalcalburnus chalcoides and hybrid were in the same cluster but Vimba vimba persa was in the other. It seems that Vimba vimba persa is a joint ancestor for these two. High heterozygosity presence in parents and their F1 shows the high potential of genetic variety in Chalcalburnus chalcoides and Vimba vimba persa which from the aquaculture management and conservation view is important and considering the high genetic variety in their hybrid, the hybrid has the sufficient potential for race improvement. Key word: Chalcalburnus chalcoides, Vimba vimba persa, hybrid. Microsatellite, Caspian Sea
Ministry of Jihad e Agriculture AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION Caspian Sea Ecology Research Center Inland Waters Aquaculture Research Center
Title : Study on inheritance of microsatellite markers in hybrid of chalcaburnus chalcoides and vimba vimba persa in southern part of Caspian Sea Apprpved Number: 4-12-12-89030 Author: Sohrab Rezvani Gilcolaei Executor : Sohrab Rezvani Gilcolaei Collaborator : Sh. Rouholahi, H. Shojaie, F. Laloei, M.J.Taghavi, M. Nayerani Advisor(s): A.Salari Location of execution : Guilan & Mazandarn provinces Date of Beginning : 2010 Period of execution : 1Year Publisher : Iranian Fisheries Research Organization Circulation : 20 Date of publishing : 2011 All Right Reserved . No Part of this Publication May be Reproduced or Transmitted without indicating the Original Reference
MINISTRY OF JIHAD - E - AGRICULTURE AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION- Caspian Sea Ecology Research Center Inland Waters Aquaculture Research Center
Title: Study on inheritance of microsatellite markers in hybrid of chalcaburnus chalcoides and vimba vimba persa in southern part of Caspian Sea
Executor : Sohrab Rezvani Gilcolaei
Registration Number 2010.1132