The National Cancer Institute at Frederick

What is it? Where is it?

Story on page 13.

NCI Acting Director Niederhuber JUNE 2006 Visits NCI-Frederick IN THIS ISSUE Recently, NCI-Frederick was Researchers in Dr. Niederhuber’s honored by an early-morning visit laboratory are using a “wounding” Science Today 3 from NCI Acting Director Dr. John model to study the effects Niederhuber, who met informally with of extracellular matrixes or NCI-Frederick 4 NCI-Frederick staff and then toured microenvironments on tumor growth NIAID’s Vaccine Pilot Plant (VPP). Platinum Publications 6 Dr. Niederhuber, a nationally Technology Transfer Branch 10 recognized surgeon and immunologist, has investigated the transcriptional Frederick Employee Diversity and translational regulation of a B- Team 11 lymphocyte protein, tyrosine kinase BLK, its role in signal transduction Poster People Profi le 12 and its oncogenic properties, and has worked on The Poster Puzzler 13 developing a Did You Know 14 mouse model for chemically induced Spring Research Festival 16 pancreatic ductal adenocarcinoma. Environment, Health, and Safety He is a prolifi c Program 18 author of scientifi c Student Intern Awards 21 papers as well as textbooks. Outreach and Special Despite his many duties as Acting Programs 22 Director, Dr. Niederhuber still carves out “escape time” for laboratory research. Campus Improvement He continues his own special interest Committee 24 in cancer stem cells and the tumor Summer Fun 25 microenvironment through his work and the role that stem cells play—how within the NCI Intramural Research the stem cells relate to the tumor Fisher BioServices 26 Program and his research program in the microenvironment—to understand the Center for Cancer Research. biology of tumor metastasis and to SAIC-Frederick, Inc. 27 With a smile, he said, “It’s important eventually identify targets to block the Data Management Services 28 to me,” and he explained that “it’s pathway activity. Under investigation also important to keep my pulse on are the conditions that favor stem Wilson Information Services what’s happening in laboratories. I cells, such as hypoxia, and therapeutic Corporation 29 think I have a better understanding of avenues that may be available by the challenges of research if I continue manipulating the tumor environment New Faces at NCI-Frederick 30 to do some research myself; that I with hypoxia-inducing factors. can better serve both intramural and Another group in his laboratory Café NCI-Frederick 31 extramural researchers if I wrestle with is working on stem cells in the Employment Opportunities 32 the same things they do each day.” continued on page 2 Dr. Niederhuber Visits NCI-Frederick continued from page 1 endocervix and studying the relation A tall man, Dr. Niederhuber loves infl uenza and anticipates the VPP will of tissue stem cells to HPV infection. basketball and played while he was at play a central role in manufacturing Detrick. However, instead of playing candidate vaccines in these programs. NCI-Frederick: for the offi cers’ team, he was a “ringer” Visionary Risk-Taking for the enlisted men’s team. “I wanted NCI-Frederick Huge When asked how NCI-Frederick to play on their team because they had Resource for the Nation could most effectively contribute a better record,” he said, laughing. Dr. Niederhuber commented about to the broad mission of NCI and Somehow, he and his teammates his visit, “It’s very impressive. NCI- the country’s initiatives in public managed to keep his offi cer status Frederick has a wonderful ability health, Dr. Niederhuber replied, hidden, and he believes his team won to help other institutes. It’s a huge “NCI-Frederick is unique in that it the championship while he was here. resource for the country, and not is managed as a Federally Funded “We had great fun playing,” he said. something that many extramural Research and Development Contract, investigators know much about, but which affords fl exibility, and is VPP Tour they need to know what we are doing a wonderful part of the overall After meeting and talking with a at NCI-Frederick.” package—visionary risk-taking.” number of NCI-Frederick researchers Taking a moment to consider his He added that NCI-Frederick and other employees, the entourage responsibilities as NCI’s Acting complements the overall intramural visited the National Institute of Director, Dr. Niederhuber concluded, and extramural programs of all of Allergy and Infectious Diseases “It’s an honor and a pleasure to be NCI; the scientists benefi t from the (NIAID) Vaccine Pilot Plant (VPP) on leading the NCI at this time; I’m really “enabling” technologies that we the outskirts of Frederick. enjoying my new role. I thought I develop and maintain. Dr. Criss Tarr, director of the VPP, knew a lot about the workings of NCI, One of Dr. Niederhuber’s dreams who led a tour around the plant, but I’m learning a lot more. Each day would be to see NCI-Frederick explained that 50% of the Vaccine brings new discoveries in science, used more and more as a national Research Center (VRC)/NIAID not just at the NCI, but all over the biomedical resource center. “We funding for developing the facility was country. I’m amazed at the quality of need to develop a way that all these for HIV, and the other half was for science and the scientists, and excited resources can be used by other biodefense and emerging infectious about the impact that we’ve had on the grantees—a team could come to diseases. The VRC is currently disease just in the last decade. NCI is NCI-Frederick, do research here, working on vaccines for HIV, Ebola, leading research in cancer, not just in then take their data back to their Marburg, West Nile virus, and the U.S., but in the world.” home environment. I’d like to lessen the barriers between intramural and extramural partnerships. Developing partnerships is very exciting for us.” Dr. Niederhuber Once Stationed at Fort Detrick Actually, April was not Dr. Niederhuber’s fi rst visit to Frederick. While in the army in the 1960s, he was stationed at Fort Detrick, doing biological research, and lived on base. He volunteered to be a “guinea pig” for many vaccines. “I was shot up so many times, I’m amazed I never became ill. And we never knew what we were getting—the vaccines had numbers, but no names,” he said with a chuckle.

The NCI-Frederick Poster 2 June 2006 Science Today

New Use for TAP: structural or catalytic (enzymatic) 2 hybrid). Both approaches have Studying Mammalian Cells proteins. The complexity of the drawbacks: the biochemical methods entire repertoire of proteins (aka the may not be sensitive or specifi c The word “tap” means different proteome) of the human is estimated to enough, and the genetic methods things to different people—a plumbing be about 1 million different molecules. tend to identify lots of false positives, fi xture, the light knocking sound causing the researcher to spend on a door produced by someone’s TAPping into Networks much time investigating false leads. knuckles. But to Dr. Deb Chatterjee, How do you get from 30,000 genes These shortcomings were the driving Associate Director of the Protein to 1 million proteins? Each gene force that led to Drs. Chatterjee and Expression Laboratory (PEL) of the can generate diversity at the RNA Kaczmarczyk’s ongoing development Research Technology Program (RTP), level through alternative splicing, of tandem affi nity purifi cation (TAP). TAP stands for Tandem Affi nity and each splice variant can in turn Purifi cation, an emerging analytical be post-translationally modifi ed by technique to study the workings of adding different types and numbers of cancer cells in a new way. sugar molecules and by sculpting the Understanding how normal and protein backbone itself with exo- and cancer cells operate requires, among endopeptidases. These processes can other things, knowing which proteins generate the tremendous diversity inside the cells associate with each of cell types in the human through other. The networks of communication differential expression of the 30,000 inside cells rely on specifi c proteins genes, the basic details of which are fi nding each other, binding and being still being deciphered by molecular modifi ed, leaving and fi nding other developmental biologists. But how partners, or causing their binding does each cell type organize the tens partners to continue carrying chemical of thousands of proteins and their messages to new proteins. A richer activities? By organizing the proteins knowledge of these communication into networks. “pipelines” in cancer cells may yield Through the work of many opportunities to intervene with drugs researchers, we have learned that Schematic diagram of Tandem Affi nity and disrupt corrupted networks or the proteome of each cell type is Purifi cation (TAP). restore those that have gone awry. organized into networks. Proteins TAP is a tool to study these protein- “communicate” with one another Think of TAP (see fi gure) as a protein interactions, but until now TAP through close physical contact network-building tool. For example, has been most intensively applied to or interaction. Researchers have if you’re interested in what causes yeast cells (which don’t get cancer). developed both laboratory and prostate cancer, one starting point for However, over the past year, Dr. computational methods to understand a diagnostic or treatment gene target Chatterjee and his colleague, Dr. Stan the composition of each network could be a prostate cell component, Kaczmarczyk, have improved TAP so and the interaction of individual e.g., the androgen receptor (AR), a that it can be applied to mammalian networks with other networks. The nuclear protein receptor that plays a cells in a rigorous, highly parallel way. proteins of a particular cell type may role in turning on the transcription of Molecular biology is based on the contain hundreds of networks, each particular genes in the cell nucleus following tenets: comprising 10 to 20 proteins. of a prostate cell. Once one of the 1) All organisms contain genes How do you study protein– AR-interacting proteins binds to AR, encoded by nucleic acid. For example, protein interaction or inter-protein AR is activated and is translocated the human genomic sequence reveals communication? Historically, protein into the nucleus, binds to a specifi c approximately 30,000 different genes. interactions have been studied genomic location (Androgen Response 2) Each gene is transcribed to using biochemical approaches (e.g., Element, ARE), and specifi cally form an RNA molecule (messenger, using antibodies to pull a particular stimulates mRNA production. ribosomal, micro). protein down from a cell extract and 3) Messenger ribonucleic acids examining what else comes along for continued on page 5 or mRNAs can be translated into the ride) or genetic methods (yeast

The NCI-Frederick Poster 3 June 2006 NCI-Frederick: Helping Others in Biodefense

NCI-Frederick: Helping BDP Aids USAMRIID at the same time. That was pretty Others in Biodefense much unheard of at the time, but it’s [Editor’s note: The following article is In 1998 Dr. Robert Ulrich, chief a tremendous way to be able to get adapted from an article by Karen Fleming- of the Department of Immunology a large number of agents into early Michael, Public Affairs Specialist, U.S. Army at USAMRIID, enlisted the clinical trials,” Dr. Reynolds said. Medical Research and Materiel Command, Biopharmaceutical Development Being able to quickly produce and which appeared in the Fort Detrick Standard, Program (BDP) at NCI-Frederick test the potential of many drugs, April 13, 2006.] to create pilot lots of a recombinant vaccines, and therapies, they believed, A warehouse-sized plant for making vaccine to protect warfi ghters from would help weed out the promising biodefense and HIV vaccines, and SEB, the second most common source from the not-so-promising products three of the Army’s biodefense of food poisoning outbreaks, and the so commercial companies would be vaccines owe at least part of their cause of staphylococcal toxic shock willing to take them on to further successes to NCI-Frederick’s syndrome. SEB has long been on the testing and FDA licensure for cancer willingness to lend a hand. list of bioterror agents because of patients. Typically, 5 to 10 percent of NCI-Frederick’s expertise in creating its ability to incapacitate or kill 80 proposed products make it to FDA small batches of drugs and vaccines percent or more of people exposed to licensure, so the more that can be for clinical trials aided the U.S. an aerosol form of it. vetted, the more that can help cancer Army Medical Research Institute of “At the time (1998) there were very patients. The NCI-Frederick program Infectious Diseases (USAMRIID) few options for making the vaccine. works on an average of 10 to 12 in getting pilot lots of vaccines They [NCI-Frederick] were one of projects a year to increase their odds for anthrax, Venezuelan equine the few games in town interested in of success. encephalitis (VEE) and staphylococcal working with us,” Dr. Ulrich said. “It enterotoxin B (SEB). NCI-Frederick’s was sort of a two-way inquiry: They Collaborations Mutually experience in building the facility wanted to build the facility up and Benefi cial where those lots are produced (the Alhough the BDP Biopharmaceutical Development and USAMRIID have Program) also helped the National very different missions, Institute of Allergy and Infectious their collaborations Diseases (NIAID) develop its own have been benefi cial. contractor-operated 126,000-square- The Army got the three foot Vaccine Pilot Plant. products it ordered from a close-by neighbor, and the NCI program, which today employs more than 125 people and has 40,000 square feet of lab space, broadened its collaborate with us, and portfolio of biopharmaceuticals. we had three projects: the recombinant SEB, the “We’re quite open to doing what we recombinant protective can for people because it feeds back antigen for Bacillus into our main mission.” anthracis, and VEE vaccine.” “It’s amazing how far the science “Our feeling has always been that if The BDP began in the 1980s to goes beyond the boundaries of a we can help serve the needs of other produce multiple biopharmaceuticals, particular disease or application,” government agencies, we should do like monoclonal antibodies and Dr. Stephen Creekmore said. Dr. it,” said Dr. Craig Reynolds, associate cytokines. “We really felt that with Creekmore is chief of the Biological director of NCI-Frederick. the proper policies, procedures and Resources Branch, which manages the protections, we could, in a single BDP. “We’re quite open to doing what facility, produce multiple agents we can for people because it feeds

The NCI-Frederick Poster 4 June 2006 NCI-Frederick: Helping Others in Biodefense back into our main mission. I can’t tell Because NCI-Frederick had the National Interagency Biodefense you how much we learned from that already established current good Campus (NIBC) being built at Fort rPA (recombinant protective antigen) manufacturing practices, or cGMP, Detrick. In fact, the NCI-Frederick project. Huge amounts.” NIAID turned to that institute for is considered a key member of a Offering the BDP’s surplus capacity help. “In many ways, NCI is a key confederation along with all the to the biodefense institute also made pioneer in the fi eld of government- campus partners. “There’s no good business sense. Dr. Reynolds owned cGMP space, and the bottom doubt that these demonstrations of said, “When we have a time when line is that we wanted to build on their interagency cooperation have really something isn’t lined up, we always success,” Dr. Gomez said. “A lot of helped to feed the NIBC,” he said. have a list of other products that can the infrastructure that we accessed was “Our collocation with the campus and go in the queue.” established by NCI as part of the BDP, the agencies will be a tremendous and we were able to leverage that. It advantage to us. It will be to our Expertise Shared with NIAID has been a very fruitful relationship.” advantage to have been here and been The NCI-Frederick also shared its Unlike the BDP, NIAID’s Vaccine a productive partner.” “how to” expertise with the National Pilot Plant doesn’t expect to be able Institute of Allergy and Infectious to offer up its services Diseases when that institute began to outside customers. building the Vaccine Pilot Plant as part “Right now we’re of its Vaccine Research Center. “The focused on developing VRC’s philosophy of accelerating our own products, timelines for vaccine development is and there is no excess facilitated by the organizational focus capacity. In the future, of the VRC, the integration of bench, should circumstances translational and clinical research allow, the pilot plant under one roof, and the ability to will be available for reliably access contract manufacturing products of interest capacity, whether via outsourcing to to the VRC, via VRC vendors, or via the new Vaccine Pilot collaborations,” Dr. Plant,” said Dr. Phil Gomez, director Gomez said. of Vaccine Production at NIAID’s Dr. Reynolds believes these Vaccine Research Center. collaborations spurred the creation of continued from page 3 column. Secondary bits of proteins are by replacing much shorter and better Hybrid AR Molecule included in the AR fusion to ensure purifi cation handles for ease of use. Demonstrates Use of TAP that AR has in fact been pulled out. The TAP technology, which may The interacting proteins can also ultimately become useful to the By constructing a hybrid AR be identifi ed following the affi nity research community as a core service, molecule (depicted in the fi gure column by gently separating the was developed from the SAIC on page 3), Drs. Chatterjee and proteins in the complex and sizing Technology Development Fund, with Kaczmarczyk have shown that TAP them by liquid chromatography additional funding from the Offi ce of can be used to “fi sh out” interacting and mass spectrometry. Using this the Director of Scientifi c Operations. proteins using any starting protein. approach, several known interacting TAP and other PEL services such In their experiment, they included proteins have been confi rmed and a as gene cloning, protein expression/ additional protein pieces from other number of new, interesting proteins purifi cation, and microbial and proteins to fi sh out the AR “bait” and were identifi ed. Currently, PEL is eukaryotic cell propagation services can AR interacting proteins. For instance, collaborating with NCI scientists Drs. be accessed by contacting Dr. James a synthetic peptide from protein A Ettore Appella, Amar Klar, and Jorge Hartley, Director of the PEL, 301-846- (two copies) is attached to AR in Toro to identify interacting proteins 7374, [email protected], or Dr. Deb this example to allow its affi nity using this TAP technology. The Chatterjee at PEL, Building 327, 301- purifi cation on an IgG-Sepharose technology is being further fi ne-tuned 846-6893 ([email protected]).

The NCI-Frederick Poster 5 June 2006 Platinum Highlight

Dr. Amar J.S. Klar differs from the new strand because Gene Regulation and Chromosome Biology Laboratory, of a somatically installed epigenetic Center for Cancer Research imprint that affects gene regulation. According to Dr. Klar, “To 10 years at the Cold Spring Harbor accomplish cellular differentiation Laboratory, where he became by the yeast paradigm, the diploid director of the Delbruck Laboratory organisms must evolve a nonrandom in 1985. In 1988, he joined NCI- strand-sorting process during mitosis.” Frederick as a Senior Scientist. His article describes his group’s Presently he holds the Senior fi nding “that such a chromosome- Biomedical Services position. specifi c, cell-type-regulated, selective- Dr. Klar’s work involves versus-random strand segregation gene silencing and mating-type mechanism indeed exists as an switching in yeast. His group ordinary cellular process in mouse determined that sister cells of cells.” He notes that “this mechanism fi ssion yeast differ from each might explain stem cell division and other developmentally, simply general cellular differentiation, as well because one daughter cell inherits as left/right axis differentiation of our Dr. Amar Klar earned his PhD in the older “Watson” DNA strand, brain hemispheres and visceral organs, the fi eld of microbiology from the and the other one inherits the older by mediating critical asymmetric cell University of Wisconsin at Madison, “Crick” strand. The older strand divisions in respective tissues during in 1977. He was a staff member for embryogenesis.”

Athanasios Armakolas and Amar J. S. Klar Cell Type Regulates Selective Segregation of Mouse Chromosome 7 DNA Strands in Mitosis Science 311(5764):1146–1149, 2006 Both older “Watson” (W) strand–containing chromatids are segregated to one daughter After chromosome replication, sister for cellular differentiation and for cell, and both older “Crick” chromatid copies are generally thought evolving strategies for developmental (C)–containing ones go to the to segregate randomly to daughter mechanisms. other daughter cell in mitosis cells. However, sister chromatids of embryonic stem cells differ in their DNA strands, with and endoderm mouse each chromatid inheriting one older cells. So, the WW:CC strand that is paired to a newly pattern is discovered. synthesized strand. Genetic analysis W strands are thick with a homologous chromosome lines; C are thin; pair indicated nonrandom chromatid older are continuous; distribution in embryonic stem cells. and younger ones are A biased segregation pattern was unfi lled. also found in all 100 endoderm cells examined, but not in any of the 165 neuroectoderm cells. In contrast, the mesoderm, cardiomyocyte, and pancreatic cells exhibited a random mode of segregation. Strand distribution mechanisms regulated For complete article and details on the fi gure, go to by cell type may have consequences http://www.sciencemag.org/cgi/content/full/311/5764/1146.

The NCI-Frederick Poster 6 June 2006 Platinum Publications

The following 54 articles have been selected Suh HC, Gooya J, Renn K, Friedman Koff WC, Johnson PR, Watkins DI, from a quarterly listing of publications in 17 of AD, Johnson PF, Keller JR. C/EBPα Burton DR, Lifson JD, Hasenkrug KJ, the most prestigious science journals. determines hematopoietic cell fate in McDermott AB, Schultz A, Zamb TJ, multipotential progenitor cells by inhibit- Boyle R, Desrosiers RC. HIV vaccine ing erythroid differentiation and inducing design: insights from live attenuated SIV Applied Biological Sciences myeloid differentiation. Blood 2006. vaccines. Nat Immunol 7(1):19–23, 2006. Edgar R, McKinstry M, Hwang J, Oppenheim AB, Fekete RA, Giulian Kottilil S, Shin K, Jackson JO, Reitano G, Merril C, Nagashima K, Adhya S. Cellular Immunology and KN, O’Shea MA, Yang J, Hallahan CW, High-sensitivity bacterial detection using Immune Regulation Lempicki R, Arthos J ,Fauci AS. Innate immune dysfunction in HIV infection: biotin-tagged phage and quantum-dot Hel Z, Tsai WP, Tryniszewska E, Nacsa effect of HIV envelope-NK cell interac- nanocomplexes. Proc Natl Acad Sci USA J, Markham PD, Lewis MG, Pavlakis tions. J Immunol 176(2):1107–1114, 2006. 103(13):4841–4845, 2006. GN, Felber BK, Tartaglia J, Franchini G. Improved vaccine protection from simian AIDS by the addition of nonstruc- Biochemistry tural simian immunodefi ciency virus Immunobiology Li M, Laco GS, Jaskolski M, Rozycki genes. J Immunol 176(1):85–96, 2006. Khan T, Hixon JA, Stauffer JK, Lin- J, Alexandratos J, Wlodawer A, Gust- coln E, Back TC, Brenner J, Lockett S, china A. Crystal structure of human T-cell Wright HV, Bailey D, Kashyap M, Nagashima K, Powell D, Wigginton JM. leukemia virus protease, a novel target for Kepley CL, Drutskaya MS, Nedospasov Therapeutic modulation of Akt activity anticancer drug design. Proc Natl Acad Sci SA, Ryan JJ. IL-3-mediated TNF produc- and antitumor effi cacy of interleukin-12 USA 102(51):18332–18337, 2005. tion is necessary for mast cell develop- against orthotopic murine neuroblastoma. ment. J Immunol 176(4):2114–2121, 2006. J Natl Cancer Inst 98(3):190–202, 2006.

Cell Biology Zhang N, Yang D, Dong H, Chen Q, Ortaldo JR, Winkler-Pickett R, Wiggin- ton J, Horner M, Bere EW, Mason AT, Acharya U, Edwards MB, Jorquera Dimitrova DI, Rogers TJ, Sitkovsky M, Bhat N, Cherry J, Sanford M, Hodge RA, Silva H, Nagashima K, Labarca P, Oppenheim JJ. Adenosine A2a receptors DL, Young HA. Regulation of ITAM-pos- Acharya JK. Drosophila melanogaster induce heterologous desensitization of itive receptors: role of IL-12 and IL-18. scramblases modulate synaptic transmis- chemokine receptors. Blood 2006. Blood 107(4):1468-1475, 2006. sion. J Cell Biol 173(1):69–82, 2006. Zhang R, Lifson JD, Chougnet C. Armakolas A, Klar AJS. Cell type Evolution Failure of HIV-exposed CD4(+) T cells regulates selective segregation of mouse Johnson WE, Eizirik E, Pecon-Slattery to activate dendritic cells is reversed by chromosome 7 DNA strands in mitosis. J, Murphy WJ, Antunes A, Teeling E, restoration of CD40/CD154 interactions. Science 311(5764):1146–1149, 2006. O’Brien SJ. The Late Miocene radiation Blood 107(5):1989–1995, 2006. of modern Felidae: A genetic assessment. Chen B, Mariano J, Tsai YC, Chan AH, Science 311(5757):73–77, 2006. Cohen M, Weissman AM. The activity of a human endoplasmic reticulum-associat- Immunology ed degradation E3, gp78, requires its Cue HIV Cicala C, Arthos J, Martinelli E, Censo- plano N, Cruz CC, Chung E, Selig SM, domain, RING fi nger, and an E2-binding Frahm N, Kiepiela P, Adams S, Linde Van Ryk D, Yang J, Jagannatha S, Chun site. Proc Natl Acad Sci USA 103(2):341– CH, Hewitt HS, Sango K, Feeney ME, TW, Ren P, Lempicki RA, Fauci AS. 346, 2006. Addo MM, Lichterfeld M, Lahaie MP, R5 and X4 HIV envelopes induce distinct Pae E, Wurcel AG, Roach T, St John gene expression profi les in primary periph- Horikawa L, Chiang YJ, Patterson T, MA, Altfeld M, Marincola FM, Moore eral blood mononuclear cells. Proc Natl Feigenbaum L, Leem SH, Michishita E, C, Mallal S, Carrington M, Heckerman Acad Sci USA 103(10):3746–3751, 2006. Larionov V, Hodes RJ, Barrett JC. Dif- D, Allen TM, Mullins JI, Korber BT, ferential cis-regulation of human versus Goulder PJR, Walker BD, Brander C. Yao ZJ, Cui YZ, Watford WT, Bream mouse TERT gene expression in vivo: Control of human immunodefi ciency virus JH, Yamaoka K, Hissong BD, Li D, Identifi cation of a human-specifi c repres- replication by cytotoxic T lymphocytes Durum SK, Jiang QO, Bhandoola A, sive element. Proc Natl Acad Sci USA targeting subdominant epitopes. Nat Im- Hennighausen L, O’Shea JJ. Stat5a/b are 102(51):18437–18442, 2005. munol 7(2):173–178, 2006. essential for normal lymphoid develop- ment and differentiation. Proc Natl Acad Sci USA 103(4):1000–1005, 2006.

The NCI-Frederick Poster 7 June 2006 Platinum Publications

Mechanisms of Signal Mantena SR, Kannan A, Cheon YP, Molecular Biology, Pathology, Transduction Li QX, Johnson PF, Bagchi IC, Bagchi and Genetics MK. C/EBP beta is a critical mediator Chen KQ, Iribarren P, Hu JY, Chen JH, Chiang YJ, Nguyen ML, Gurunathan of steroid hormone-regulated cell prolif- Gong WH, Cho EH, Lockett S, Dunlop S, Kaminker P, Tessarollo L, Campisi J, eration and differentiation in the uterine NM, Wang JM. Activation of toll-like re- Hodes RJ. Generation and characteriza- epithelium and stroma. Proc Natl Acad Sci ceptor 2 on microglia promotes cell uptake tion of telomere length maintenance in USA 103(6):1870–1875, 2006. of Alzheimer disease-associated amyloid tankyrase 2-defi cient mice. Mol Cell Biol beta peptide. J Biol Chem 281(6):3651– 26(6):2037–2043, 2006. Nanda A, Karim B, Peng ZS, Liu 3659, 2006. GS, Qiu WP, Gan C, Vogelstein B, St Fong LG, Ng JK, Lammerding J, Vick- Croix B, Kinzler KW, Huso DL. Tumor Lee HS, Bong YS, Moore KB, Soria K, ers TA, Meta M, Cote N, Gavino B, Qiao endothelial marker 1 (Tem1) functions Moody SA, Daar IO. Dishevelled medi- X, Chang SY, Young SR, Yang SH, Stew- in the growth and progression of ab- ates ephrinB1 signaling in the eye fi eld art CL, Lee RT, Bennett CF, Bergo MO, dominal tumors. Proc Natl Acad Sci USA through the planar cell polarity pathway. Young SG. Prelamin A and lamin A appear 103(9):3351–3356, 2006. Nat Cell Biol 8(1):55–63, 2006. to be dispensable in the nuclear lamina. J Clin Invest 116(3):743–752, 2006. Ott MG, Schmidt M, Schwarzwaelder Li WQ, Jiang Q, Aleem E, Kaldis P, K, Stein S, Siler U, Koehl U, Glimm H, Khaled AR, Durum SK. IL-7 promotes Gold B, Merriam JE, Zernant J, Kuhlcke K, Schilz A, Kunkel H, Naun- T-cell proliferation through destabilization Hancox LS, Taiber AJ, Gehrs K, dorf S, Brinkmann A, Deichmann A, of p27Kip1. J Exp Med 2006. Cramer K, Neel J, Bergeron J, Barile Fischer M, Ball C, Pilz I, Dunbar C, Du GR, Smith RT, Hageman GS, Dean M, Y, Jenkins NA, Copeland NG, Luthi U, Pandhare J, Cooper SK, Phang JM. Allikmets R, Chang S, Yannuzzi LA, Hassan M, Thrasher AJ, Hoelzer D, von Proline oxidase, a proapoptotic gene, is in- Merriam JC, Barbazetto I, Lerner Kalle C, Seger R, Grez M. Correction of duced by troglitazone—Evidence for both LE, Russell S, Hoballah J, Hageman J, X-linked chronic granulomatous disease peroxisome proliferator-activated recep- Stockman H. Variation in factor B (BF) by gene therapy, augmented by insertional tor gamma-dependent and -independent and complement component 2 (C2) genes activation of MDS1-EVI1, PRDM16 or mechanisms. J Biol Chem 281(4):2044– is associated with age-related macular SETBP1. Nat Med 12(4):401–409, 2006. 2052, 2006. degeneration. Nat Genet 2006.

Yu Q, Park JH, Doan LL, Erman B, Hanson JA, Gillespie JW, Grover A, Feigenbaum L, Singer A. Cytokine signal Membrane Transport, Structure, Tangrea MA, Chuaqui RF, Emmert- transduction is suppressed in preselec- Function, and Biogenesis Buck MR, Tangrea JA, Libutti SK, tion double-positive thymocytes and Gattis JL, Washington AV, Chisholm Linehan WM, Woodson KG. Gene restored by positive selection. J Exp Med MM, Quigley L, Szyk A, McVicar promoter methylation in prostate tumor- 203(1):165–175, 2006. DW, Lubkowski J. The structure of the associated stromal cells. J Natl Cancer extracellular domain of triggering receptor Inst 98(4):255–261, 2006. expressed on myeloid cells like transcript- Medical Sciences 1, and evidence for a naturally occurring Hruban RH, Adsay NV, Albores-Saave- Helgadottir A, Manolescu A, Helgason soluble fragment. J Biol Chem 2006. dra J, Anver MR, Biankin AV, Boivin A, Thorleifsson G, Thorsteinsdottir U, GP, Furth EE, Furukawa T, Klein A, Gudbjartsson DF, Gretarsdottir S, Mag- Mattera R, Tsai YC, Weissman AM, Klimstra DS, Kloppel G, Lauwers GY, nusson KP, Gudmundsson G, Hicks A, Bonifacino JS. The Rab5 guanine nucleo- Longnecker DS, Luttges J, Maitra Jonsson T, Grant SFA, Sainz J, O’Brien tide exchange factor Rabex-5 binds ubiq- A, Offerhaus GJA, Perez-Gallego L, SJ, Sveinbjornsdottir S, Valdimars- uitin (Ub) and functions as a Ub ligase Redston M, Tuveson DA. Pathology of son EM, Matthiasson SE, Levey AI, through an atypical Ub-interacting motif genetically engineered mouse models of Abramson JL, Reilly MP, Vaccarino and a zinc fi nger domain. J Biol Chem pancreatic exocrine cancer: consensus V, Wolfe ML, Gudnason V, Quyyumi 281(10):6874–6883, 2006. report and recommendations. Cancer Res AA, Topol EJ, Rader DJ, Thorgeirsson 66(1):95–106, 2006. G, Gulcher JR, Hakonarson H, Kong A, Stefansson K. A variant of the gene Microbiology encoding leukotriene A4 hydrolase confers Buck CB, Day PM, Thompson CD, ethnicity-specifi c risk of myocardial in- Lubkowski J, Lu WY, Lowy DR, Schil- farction. Nat Genet 38(1):68–74, 2006. ler JT. Human alpha-defensins block papillomavirus infection. Proc Natl Acad Sci USA 103(5):1516–1521, 2006. The NCI-Frederick Poster 8 June 2006 Platinum Publications

Reilly KM, Broman KW, Bronson RT, Oncogene Wexler-Cohen Y, Johnson BT, Puri Tsang S, Loisel DA, Christy ES, Sun Hara T, Abe M, Inoue H, Yu LR, A, Blumenthal R, Shai Y. Structur- ZH, Diehl J, Munroe DJ, Tuskan RG. Veenstra TD, Kang YH, Lee KS, Miki ally altered peptides reveal an important An imprinted locus epistatically infl uences T. Cytokinesis regulator ECT2 changes role for N-terminal heptad repeat bind- Nstr1 and Nstr2 to control resistance to its conformation through phosphoryla- ing and stability in the inhibitory action nerve sheath tumors in a neurofi broma- tion at Thr-341 in G2/M phase. Oncogene of HIV-1 peptide DP178. J Biol Chem tosis type 1 mouse model. Cancer Res 25(4):566–578, 2006. 281(14):9005–9010, 2006. 66(1):62–68, 2006. Liu Y, Borchert GL, Surazynski A, Hu Waalkes MP, Liu J, Ward JM, Powell CA, Phang JM. Proline oxidase activates Protein Synthesis, DA, Diwan BA. Urogenital carcinogen- both intrinsic and extrinsic pathways for Post-translation Modifi cation, esis in female CD1 mice induced by in apoptosis: the role of ROS/superoxides, and Degradation utero arsenic exposure is exacerbated NFAT and MEK/ERK signaling. Onco- Chen ZQ, Dong J, Ishimura A, Daar I, by postnatal diethylstilbestrol treatment. gene 2006. Cancer Res 66(3):1337–1345, 2006. Hinnebusch A, Dean M. The essential vertebrate ABCE1 protein interacts with eu- Ishimura A, Lee HS, Bong YS, Saucier karyotic initiation factors. J Biol Chem 2006. C, Mood K, Park EK, Daar IO. Onco- Neoplasia genic Met receptor induces ectopic struc- Mitchell MS, Tozser J, Princler G, Boyd KE, Xiao YY, Fan K, Poholek A, tures in Xenopus embryos. Oncogene 2006. Lloyd PA, Auth A, Derse D. Synthesis, Copeland NG, Jenkins NA, Perkins AS. processing, and composition of the virion- Sox4 cooperates with Evi1 in AKXD-23 Sterneck E, Zhu S, Ramirez A, Jorcano associated HTLV-1 reverse transcriptase. myeloid tumors via transactivation of pro- JL, Smart RC. Conditional ablation of J Biol Chem 281(7):3964–3971, 2006. viral LTR. Blood 107(2):733–741, 2006. C/EBP beta demonstrates its keratino- cyte-specifi c requirement for cell survival Herranz M, Martin-Caballero J, and mouse skin tumorigenesis. Oncogene RNA: Structure, Metabolism, Fraga MF, Ruiz-Cabello J, Flores JM, 25(8):1272–1276, 2006. Desco M, Marquez V, Esteller M. The and Catalysis novel DNA methylation inhibitor zebu- Dash C, Marino JP, Le Grice SFJ. Ex- larine is effective against the develop- Phagocytes amining Ty3 polypurine tract structure and function by nucleoside analog interference. ment of murine T-cell lymphoma. Blood Biswas SK, Gangi L, Paul S, Schioppa J Biol Chem 281(5):2773–2783, 2006. 107(3):1174–1177, 2006. T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago Gan J, Tropea JE, Austin BP, Court Yang HS, Matthews CP, Clair T, Wang L, Nebuloni M, Mantovani A, Sica DL, Waugh DS, Ji X. Structural insight Q, Baker AR, Li CCH, Tan TH, Colburn A. A distinct and unique transcriptional into the mechanism of double-stranded NH. Tumorigenesis suppressor Pdcd4 program expressed by tumor-associated RNA processing by ribonuclease III. Cell down-regulates mitogen-activated protein macrophages (defective NF-kappa B and 124(2):355–366, 2006. kinase kinase kinase kinase 1 expression enhanced IRF-3/STAT1 activation). Blood to suppress colon carcinoma cell invasion. 107(5):2112–2122, 2006. Mol Cell Biol 26(4):1297–1306, 2006. Transplantation Protein Structure and Folding Welniak LA, Kuprash DV, Tumanov Neuroscience AV, Panoskaltsis-Mortari A, Blazar BR, El Omari K, Ren J, Bird LE, Bona MK, Sun K, Nedospasov SA, Murphy WJ. Garcia-Fresco GP, Sousa AD, Pillai Klarmann G, LeGrice SFJ, Stammers Peyer patches are not required for acute AM, Moy SS, Crawley JN, Tessarollo DK. Molecular architecture and ligand rec- graft-versus-host disease after myeloab- L, Dupree JL, Bhat MA. Disruption of ognition determinants for T4 RNA ligase. lative conditioning and murine alloge- axo-glial junctions causes cytoskeletal dis- J Biol Chem 281(3):1573–1579, 2006. organization and degeneration of Purkinje neic bone marrow transplantation. Blood 107(1):410–412, 2006. neuron axons. Proc Natl Acad Sci USA Prabakaran P, Gan J, Feng Y, Zhu Z, 103(13):5137–5142, 2006. Choudhry V, Xiao X, Ji X, Dimitrov DS. Structure of SARS coronavirus receptor- binding domain complexed with neutral- izing antibody. J Biol Chem 2006.

The NCI-Frederick Poster 9 June 2006 Technology Transfer Branch (TTB)

It’s a Small Patent deceptive intent, and if disclosure you’re going down the roller coaster World after All of the invention through a foreign instead of up it now? application wouldn’t be detrimental Welcome back to the wonderful to national security (35 U.S.C. §181 world of patenting. As you may Secrecy of certain inventions and Twist #3: Informal recall from previous Technology withholding of patent), patent law Collaboration Agreements Transfer Branch articles published in provides a process for granting of For those who prefer the smoother the September and December 2005 a retroactive foreign fi ling license. ride of a merry-go-round or water NCI-Frederick Poster, the diagram Without it, your new discovery may fl ume to a monster roller coaster, for following and understanding the not be eligible for patent protection TTB suggests keeping lines of patenting process is not unlike the in the U.S. The only way to “lift the communication open and active with maps of some well known amusement bar” is to obtain a retroactive foreign foreign collaborators, especially in parks: As much as one may study the fi ling license by fi ling a petition with the patent process. When entering map or diagram, the translation of the U.S. Patents and Trademarks into even an informal collaboration, what is on the paper to what actually Offi ce and hope such will be granted, put an agreement in place that “is” can be quite an eye-opening which is a long, costly, and legally speaks to patenting. NCI has a new experience. And even if one has arduous process for the inventor and Collaboration Agreement (CA) that managed to maneuver through one the inventor’s employer (35 U.S.C. is perfect for informal collaborations area of the park or process without too §185 Patent barred for fi ling without with language agreeing to work much trepidation, i.e., the kiddy rides license). The U.S. inventor faces other together on publication and patenting. or U.S. patent process, the experience complications if an application is fi led One of the terms of the CA says: can change from easygoing to the in a foreign country without a foreign The Parties agree to work together hair-raising twists and turns of a roller fi ling license if the inventor’s failure to to make the results of their research coaster ride with little warning. obtain the license was not due to error publicly available; however, before or was with deceptive intent. These either Party submits a paper or complications may include criminal abstract for publication, the other Twist #1: Need for a Foreign charges and fi nes. This would be the Party shall have thirty (30) days to Filing License hair-raising part of the ride. review the proposed publication to Here is a real-world example: ensure that Confi dential Information The NCI-Frederick Laboratory of is protected. The disclosing Party may Cure-for-Cancer is involved in an request in writing that the proposed informal collaboration with a foreign Twist #2: Differences between publication be delayed for up to thirty university. The foreign university U.S. and Foreign Patent Laws (30) additional days as necessary to located in Azerbaijan wants to fi le a Another element U.S. inventors fi le a patent application. Data that are Azerbaijanian patent application on a want to be aware of is the difference generated by either Party under the discovery that was made during your between U.S. patent law and foreign Research Plan (“Research Data”) will collaboration. Your Azerbaijanian patent laws in naming inventors on be kept confi dential until published collaborator graciously includes your applications. In the U.S., inventorship or a corresponding patent application name as an inventor. This may seem is a legal determination; the application has been fi led. harmless enough, until you realize a must include all inventors who made The CA may be just the ticket to foreign fi ling license was not obtained. independent, conceptual contributions keep your research and resulting Under U.S. patent law, a foreign- to the invention. In many foreign inventions on the fast track without fi ling license must be obtained from countries, inventorship is more of a too many unexpected twists and turns. the United States Patent Offi ce business or good-will decision than Your laboratory’s NCI Technology Commissioner of Patents prior to a legal decision. Therefore, a foreign Transfer Specialist can help you put a fi ling a patent application in a foreign colleague may think naming a U.S. CA or other appropriate transactional country on an invention made in collaborator as an inventor on a foreign agreement in place to make the most the U.S.A. (35 U.S.C. §184 Filing application is an act of good will, not of your collaborative efforts and to of application in foreign country). realizing he or she may actually be protect you and your inventions as you If a foreign patent application is creating a patent law nightmare for enjoy your adventure in the wonderful fi led through error and without the American collaborator. Feel like world of patenting!

The NCI-Frederick Poster 10 June 2006 Frederick Employee Diversity Team

Honey and Diversity

Since last month’s Spring Research Melissa plundered bee hives of Festival was symbolized by the their honey to sustain Zeus. When honeybee, we thought you might enjoy Melissa’s role in protecting Zeus was reading a bit about the use of honey in discovered, Cronos transformed her cultures around the world. into an earthworm. Zeus later took In many cultures, honey has pity on Melissa and transformed her associations that go far beyond its into a honeybee. This mythology is use as a food. In literature, religion evidenced in the scientifi c name for and folk belief, honey is frequently a honeybees, Apis meliffera. symbol of human or godly kindness, Even the Bard had words to say or a symbol for that which is pleasant about honey: “He is not worthy and desirable. Those knowledgeable of the honeycomb, that shuns the about Judeo-Christian culture may hives because the bees have stings” recall that Moses’ Promised Land is (Shakespeare, William. Locrine, described as “fl owing with milk and Act III:iii). honey.” The signifi cance of honey carries over to Jewish tradition, in that References an apparent exception was made for Bishop, Holley. Robbing the Bees. it in Jewish dietary laws: Although Free Press, 2005 Teresa Ramirez, Laboratory of insects and their products are normally Experimental Immunology, and considered unclean, honey is deemed a http://www.geocities.com/Area51/ Paul Miller, Chairperson, Frederick kosher food product. Dungeon/4785/Greek.html Employee Diversity Team, display the Honey Symbolizes Kindness Diversity Team’s cookbook and movie http://www.herbsnhoney.com/bees.htm guide. The publications were given out In Buddhist theology, the festival at the Spring Research Festival. To of Madhu Purnima in late summer http://www.ibra.org.uk/ join the Diversity Team, e-mail Paul commemorates Buddha’s making Miller at [email protected]. peace among his disciples by http://www.wsu.edu/~delahoyd/ retreating into the wilderness. Buddha shakespeare/locrine3.html was nourished in his hideaway by a monkey bringing him honey to eat. On Madhu Purnima, Buddhists remember this kindness by ceremoniously giving honey to monks. The monkey’s gift is Winners of Diversity Movie Tickets frequently depicted in Buddhist art. Vishnu, Preserver and Protector of the Win a free movie pass from the Diversity Team! Just answer the questions Hindu trinity of gods, is often depicted posted at the Diversity display case in the Café NCI-Frederick, Building 549. as a blue bee on a lotus fl ower. Congratulations to the lucky winners from February and March: Honey plays an important role in Greek mythology—one that is today Alan Brooks Tiffany Gee Fung Lian commemorated in the names not Shawn Brown Roxanne Gibson Daniel Oleyar only of humans, but bees themselves. Samitabh Chakroborti Ann Gamero Beverly Studebaker Melissa was a nymph who extended Eileen Downey Yolanda Goines Martha Summers kindnesses to the infant Zeus while Kathy Easterday Jeanne Hazzard Tim Waybright he was hidden away from his father, Erica Emeigh Chistian Kloc Alexander Zdanov Cronus, the lord of the universe.

The NCI-Frederick Poster 11 June 2006 Poster People Profi le: Fran Hostetler

What Do You Do? which means fi nding food items that Loves the People Here are low calorie and low fat, while still Frances “Fran” Hostetler fi rst came trying to keep the ‘steak-and-potato’ Interacting with people on campus to Ft. Detrick in 1983, as a soldier. people happy.” is her favorite part of her job. “The She was manager of “the old club,” absolute best part of my job is which is now the community the interaction I can have with activities center. After fi ve years the patrons who come in to at Ft. Detrick, she went to Ft. use the facility. I have had the Dix, New Jersey, where she was a opportunity to meet some of Drill Instructor for two years. She the nicest and most interesting returned to Ft. Detrick to work as a people,” she said, adding, cook at the club, and, when the Café “Without this job, I would never NCI-Frederick opened its doors in have known any of these people.” 1996, she started there as a cook. As she puts it, “The rest is history.” In Her Free Time Offi cially, she is the Facilities The owner of three standard Manager, but, she says, she does poodles, she says her three “boys” everything from mopping fl oors are her pride and joy. She shows to preparing the annual budget. “I two of them in obedience class, really try to keep the patrons happy which, she said, “has taught me by providing a variety of good, patience and humility.” Other healthy food options at a reasonable favorite activities include running price, and by providing a friendly and working out. She believes greeting and a good atmosphere to “there’s nothing like a long run enjoy their meal or break,” she said at the end of the day to relieve in a recent interview. the tension.” Trying to keep a few thousand people happy at Fran Hostetler Career Began in the Army mealtime must inspire some long Facilities Manager, Café NCI-Frederick Ms. Hostetler learned about food runs, indeed. service in the Army, having attended school in all phases of the business and taken several classes in fi nancial management while still on active duty. “I have the Army to thank for most of my education in food service,” she commented, but she indicated that experience has taught her the most. “I guess the best teacher of all was the fact that I have spent the last 40 years of my life in food service of one kind or another.” Changes in Eating Habits Created Challenges The biggest single change she has witnessed over the years is in the kind of food people are eating now. “People are now more into healthy eating,

The NCI-Frederick Poster 12 June 2006 Poster Puzzler What is it? Where is it?

Your challenge, should you decide to accept it, is to correctly identify the item and its location from the picture to the right. Clue: It’s somewhere at Fort Detrick/NCI-Frederick. Win a framed photograph of the Poster Puzzler by e-mailing your guess, along with your name, e-mail address, and daytime phone number, to Poster Puzzler at poster@ncifcrf. gov. Alternatively, you can send us your guess, along with your name and daytime phone number on one of The Poster forms found on the front of The Poster stands in the lobbies of Buildings 426 and 549. All entries must be received by Friday, July 28, 2006, and the winner will be drawn from all correct answers received by that date.

Good luck and good hunting!

The Poster Puzzler: A Piece of the Rock

The March Poster Puzzler can probably be best described by what it’s not: it’s not a picture of a mountain range taken from a hot air balloon; nor is it the fossil of a manatee that washed up from the antediluvian deep 100,000 years ago. The March Puzzler is a rock in the grassy area across from Building 538. Measuring just over fi ve feet in length, this limestone rock has lain in quiet vigil over the area for much longer than any of us have been here. In the early 1950s, it saw the construction of the U.S. Army’s Aerobiological Laboratories in Building 376, and the U.S. Army Chemical and Physical Laboratory in Building 538. Nearly 40 years later, it saw the installation of the modular buildings now housing Human Resources (Building 371) and the HR Recruiting and the NCI Ethics offi ces (Building 372). This sturdy guardian also shows signs (war wounds) of confl ict with lawn mowers over the years. Yet through it all, it has remained true and solid as a, well, rock.

Special thanks to Rocky (no pun intended) Follin, FME, for the Congratulations to our March 2006 Poster information in this article. Puzzler joint winners: Jami Troxler, Research Technician, and Michael Malasky, Research Thanks to all the participants in the March 2006 Poster Puzzler! Assistant CORE Laboratory

The NCI-Frederick Poster 13 June 2006 Did You Know?

NCI-Frederick Garners Awards

NIH Plain Language Award was scientifi c advisor to, the Werner and was the fi rst recipient of the NCI- Kirsten Student Intern Program; NIH Directors’ Award for Mentoring. NCI- established and still runs the Summer Dr. Young commented that after Frederick’s Student Seminar Series; recently reviewing the accomplishments of fi rst Annual developed a student training program the other winners, “I am honored by Report on ethics and the conduct of scientifi c the fact that I was also chosen for this Executive research (visit the Web site http://web. award. Although I knew I had been Summary ncifcrf.gov/campus/ethicscourse/); nominated, I was full of some disbelief has been and has served for three years on the when I received the call that I had awarded an NCI Introduction to Cancer Research been selected. It is a great privilege to Honorable Career selection committee. As a work here at NCI-Frederick, and my Mention mentor and member of the research attempts to improve the quality of this in the career program, Dr. Young sponsors workplace represent my commitment 2005 NIH a student and has helped select and to recognize this privilege by devoting Plain Language Awards competition. place disadvantaged minority students some of my efforts to the organization Produced in fall 2004, the 16- interested in biochemical research. as a whole.” page booklet was developed Dr. Young also brings humor to his The American Society for Public following extensive interviews with work with young people. In speaking to Administration and the National representatives from Charles River student groups, he often presents “The Academy of Public Administration Laboratories, Data Management Top 12 Rules to Remember for Working jointly present these annual awards for Services, SAIC-Frederick, Inc., and in a Laboratory.” Three are: take excellence in public service. The awards Wilson Information Services, Inc. your work seriously, but not yourself were established in 1983 “to honor The staff of Scientifi c Publications, seriously; only work with people individuals whose accomplishments Graphics & Media and other SAIC who like chocolate; and you can go are models of public service within information specialists worked closely anywhere you want if you look serious and outside the work environment with NCI-Frederick administration in and carry a rack of microfuge tubes. …[and] who exhibit the highest developing both content and design. standards of excellence, dedication, Having also achieved a Gold Award in and accomplishment over a sustained the 2005 MarCom Creative Awards and period of time and who are creative and an Award of Distinction in the 2005 skilled career managers at all levels of Communicator Print Media Awards, the public service,” according to the Web summary has now been recognized for site, http://www.napawash.org/about_ excellence in graphic design, effective academy/npsa2006winners.html. communication, and readability. Dr. Young is currently investigating new methods to combine National Public Service Award cytokine treatment with use of NCI-Frederick Principal Investigator chemotherapeutic drugs in the Dr. Howard Young, Laboratory of preclinical development of possible Experimental Immunology, received new protocols to treat cancer. He a National Public Service Award has been involved in a collaborative in April. The award recognized project with the U.S. Army Medical his efforts to improve the work Research Institute for Infectious environment of NCI-Frederick and Disease, which led to important NIH. Over the years, Dr. Young has fi ndings on the mechanisms involved been involved in a number of events to Dr. Young is a two-time recipient of in the host response to Ebola virus that end: he founded, and for fi ve years the National Institute of Health’s Merit infection and anthrax toxin. His many chaired, the Spring Research Festival; Award. He is an elected fellow in the other scientifi c accomplishments participated in the initiation of, and American Academy of Microbiology, are refl ected in more than 240 peer- reviewed papers.

The NCI-Frederick Poster 14 June 2006 Did You Know?

TITLE 4—FLAG AND SEAL, Notice the regulation includes both SEAT OF GOVERNMENT, U.S. citizens and our foreign visitors. AND THE STATES CHAPTER It is an honor to have foreign guests 1—THE FLAG training with us at NCI-Frederick. We invite our visitors to join us in Sec. 9. Conduct during hoisting, honoring our fl ag. Many visitors’ lowering or passing of fl ag. countries have suffered the loss of During the ceremony of hoisting war heroes, just as we have. As these or lowering the fl ag or when the visitors stand at attention to our fl ag, fl ag is passing in a parade or in they may think of their countries and review, all persons present except the freedoms they have that were Do You Know Your those in uniform should face the bought at such a high price. fl ag and stand at attention with the U.S. Flag Laws and right hand over the heart. Those Respecting the Flag Regulations? present in uniform should render Now, you may ask, “What about the military salute. When not in when I am driving my car?” [Editors’ note: In this “season” of Memorial uniform, men should remove their Offi cer Pineiro, NIH police, Day, Flag Day, and the Fourth of July, it headdress with their right hand explained that when we hear the bugle seems appropriate to print the article below and hold it at the left shoulder, calls, we should stop, get out of our about U.S. fl ag customs and courtesies. Since the hand being over the heart. vehicle, and salute, as noted in the fl ag we work on a military post, an occasional reminder of proper fl ag etiquette can’t hurt. Aliens should stand at attention. regulations above. Sadly, although We thank Ann Rogers, Laboratory of Cancer The salute to the fl ag in a moving Offi cer Pineiro is required, as an Prevention, for writing this article.] column should be rendered at the offi cer of the law, to render respect to moment the fl ag passes. the fl ag, since very few people here I was brought up to respect, honor, stop when “Retreat” is played, he and love our fl ag and those who have —From the U.S. Code Online via must focus his attention on the traffi c, died fi ghting to give me my freedom. GPO Access [wais.access.gpo.gov]; forcing him to forgo his duty to his I was an “Army brat” in many ways: laws in effect as of January 7, 2003; country to oversee our safety. I was born on a military installation; document not affected by public laws “Retreat” does not simply tell us both parents served in the military; enacted between January 7, 2003, and that it is 5:00 p.m. and our work day an uncle was killed in World War II; February 12, 2003 [CITE: 4USC9]. is done. Each day, as we listen to two sons served in the Armed Forces; the news about the fi ghting in Iraq, and now I work at Ft. Detrick. Thus, “Retreat” provides us the opportunity I am sensitive to the rules of military to refl ect on those making the supreme etiquette. Each day, when I hear the sacrifi ce. Often, as I listen to “Retreat” bugle calls at 5:00 p.m., I face the and “To the Colors,” I pray for those in fl ag and put my hand over my heart. the military or just focus my thoughts, Each day, I also see folks hurrying with a grateful heart, on them and their to their cars, apparently unaware of willingness to pay a high price for me. what is taking place and what their Now, when you hear the bugle response should be. (Ft. Detrick has calls, get out of your car and stand at put in speakers all over the post to attention for just those few minutes ensure that we hear “Retreat” and “To to honor and respect our fl ag. Better the Colors” being played at the fl ag- yet, watch the soldiers perform this lowering ceremony.) ceremony in front of Building 810; it will stir your heart. Flag Regulations The U.S. Code of Regulations explains our responsibility during fl ag ceremonies or when the fl ag passes us in a parade.

The NCI-Frederick Poster 15 June 2006 Spring Research Festival

Spring Research Festival Celebrates Tenth Year

On May 17 and 18, the corner of New in 2006 Ditto and Sultan swarmed with the activity of the tenth annual Spring New this year was the presence of the Research Festival. Hundreds of National Interagency Confederation vendors, exhibitors, and scientists took for Biological Research, a part in the popular event to showcase collaborative working group of all the science underway at the National the medical research and advanced Cancer Institute at Frederick and biotechnology organizations from the Fort Detrick. biotechnology and biodefense sides of the campus. The Confederation Successful Formula comprises the CDC, DHS, NCI- The Spring Research Festival has Frederick, NIAID, USAMRIID, and attracted big crowds every year since USDA. Representatives were on it began 10 years ago, when, according hand to discuss the mission of the to festival founder Dr. Howard Young, Confederation and the large, scale it drew nearly 150 vendors and 30 model of the new National Interagency exhibitors. While a few features have Biodefense Campus at Ft. Detrick. been modifi ed over the years, the basic Another new feature, according to format of the festival has remained the Ms. Parrott, was the display of all same: posters, technology, exhibits, posters on both days, an important and, of course, food. change that allowed extra time for The most signifi cant change came viewing the full range of scientifi c fi ve years ago, when, Dr. Young fi ndings. Over 160 posters were explained, the Offi ce of the Director presented, and nearly 200 vendors and adopted the festival “as a regular, 42 exhibitors from both within and established NCI-Frederick showcase outside the community participated in of the excellent science being the event. performed by the NCI, the Army, and the USDA.” This change included Other Events the selection of Ms. Cheryl Parrott as The keynote speaker was Dr. Daniel Chair of the Organizing Committee, Erlanson, of Sunesis Pharmaceuticals, which, Dr. Young noted, “provided Inc., who presented “Fragment- ‘institutional memory,’” and helped based Drug Discovery at Fluxional defi ne the responsibilities of the Sites in Proteins,” describing the use various offi ces that contribute to the of Tethering® in a fragment-based festival’s success. As an “offi cial” approach to drug discovery. The event, the festival was assured of Scientifi c Library offered movies to enough volunteers and, equally both entertain and educate viewers important, appropriate funding. about the honeybee, this year’s mascot. The festival has drawn increasing In spite of the “rinse-cycle/dry cycle” numbers every year from all across weather, Ms. Parrott commented, “We campus. Dr. Young refl ected, “I believe had wonderful attendance. This is truly that all the groups here at Ft. Detrick an all-institute success because there’s now see the Festival as an event in so much teamwork involved.” which participation is worthwhile and expected by their communities. This For more information on the 2006 was evident to me this year by the Spring Research Festival, visit the signifi cant number of posters from the Web site, http://web.ncifcrf.gov/ Army and the USDA.” events/springfest/default.asp.

The NCI-Frederick Poster 16 June 2006 Spring Research Festival

Congratulations to the 2006 Spring Research Festival Poster Winners Associate Director Dr. Ester Rozenblum — Laboratory of Molecular Technology (New Technology) Laboratory of Molecular Technology (LMT) Technology Development Associate Scientist John M. Roman — Laboratory of Protomics and Analytical Technologies (New Technology) Comparative Separation of Estrogens by HPLC-MS and SFC-MS Investigators David DeShazer — USAMRIID Bacteriology Division (Infectious Pathogens) A New Secretion System in Burkholderia mallei Is Required for Virulence and Secretion of an Immunogenic Protein Farivar M. Eskandari — USDA-ARS-Foreign Disease Weed Science Research Unit (New Technology) Discovery of a Cercosporella Leaf Spot on Russian Knapweed from Montana Postdoctoral Fellows Melissa M. McKay — Laboratory of Protein Dynamics and Signaling (Biochemistry) Effects of Caspase-dependent Cleavage of KSR1 on TNFα;–mediated apoptosis Veronica Hall — Laboratory of Experimental Immunology (Cancer Biology) Friend or Foe? IFN-Gamma Mediated Induction of Pro-Metastatic Gene Expression in the Tumor Microenvironment Dr. Cyril Berthet — Mouse Cancer Genetics Program (Developmental and Cell Biology) Combined loss of Cdk2 and Cdk4 Results in Embryonic Lethality and Rb Hypophosphorylation William C. Dunty, Jr. — Cancer and Developmental Biology Laboratory (Developmental and Cell Biology) Wnt3a/beta-catenin Signaling Regulates Mesoderm Fate and Vertebrate Segmentation by Controlling a Network of Transcription Factors Joseph C. Manimala — Laboratory of Medicinal Chemistry (Diagnostics and Therapeutics) Development of a Novel Carbohydrate Microarray and its Applications Ana Romero — Laboratory of Experimental Immunology (Immunology) Development of a Type I Interferon Resistant Clone Implicates STAT2 as a Critical Mediator in the Induction of Apoptosis. Rashmi Jalah — Vaccine Branch, CCR (Immunology) Optimization of Expression of Human and Rhesus Interleukin-15 (IL-15) for In Vivo DNA Delivery Dr. Zhen Xiao — Laboratory of Proteomics and Analytical Technologies (Molecular Biology) Analysis of the Extracellular Matrix and Vesicle Proteomes in Osteoblasts Laura Carim-Todd — Mouse Cancer Genetics Program (Molecular Biology) A Conserved Amino Acid Sequence that Increases TrkB Signaling Drs. Cassio Baptista and Kelly Banfi eld — Laboratory of Molecular Technology (New Technology) Virus-Microarray and Monoclonal Antibody-Microarray James Gattis — Macromolecular Crystallography Laboratory (Structural Biology & Chemistry) The Structure of the Immunoglobulin-like Domain of TLT-1 and Detection of a Natually Occuring Soluble Fragment Cristina Bergamaschi — Vaccine Branch, CCR (Vaccines and Gene Therapy) Modulation of Immunogenicity of SIV and HIV Antigens Using Fusion Proteins with Lysosomal-Associated Membrane Protein-1 (LAMP-1) J.Mohamad Fakruddin — Laboratory of Human Retrovirology (Virology) Identifi cation of Novel Anti-HIV-1 Role for IL-27 Hongzhan Xu — HIV Drug Resistance Program (Virology) Identifi cation of APOBEC3G Mutants that Exhibit a Defect in Virion Incorporation Dr. Galina N. Nikolenko — HIV Drug Resistance Program (Virology) A Novel Mechanism of HIV-1 Drug Resistance: Mutations in the Reverse Transcriptase Connection Domain Enhance AZT Resisitance. Olga A. Nikolaitchik — HIV Drug Resistance Program (Virology) Effect of GAG Mutations on Recombination and RNA Packaging in HIV-1 Research Associates Erik Harris — Laboratory of Functional Genomics (Drug Development and Delivery) A Sterol Mesylate Activator of CEBPa Signaling Induces Monocytic Differentiation in Human Leukemia Cells In Vitro and In Vivo. Senior Research Associate Claudia Stewart — Laboratory of Molecular Technology (Informatics) LMT LIMS continued on page 31 The NCI-Frederick Poster 17 June 2006 Environment, Health, and Safety Program

How Safe Is Your provide safety information to their lab continually changes, it is essential that Laboratory? personnel. In addition to taking safety laboratory staff be commensurately seriously, safe labs have these items in trained in new technical challenges Is your laboratory safe? If you said common: and any changes in the potential risks yes, how do you know it is safe? Would associated with their work. others in your lab agree? Do you think • Environment, Health, and Safety However, suppose a lab supervisor someone from EHS would agree? rules and procedures are followed; stresses to a technician the need to recover higher yields of viral particles. Answer this Question • Pathogen and rDNA registrations, To achieve the higher yields, the (Honestly!) to Determine If alarm notifi cations, safety plans, technician decides to change the Your Lab Is Safe: etc., are current and accurate; standard laboratory procedure to Is everyone who works in your include the use of sharps. While this lab thoroughly familiar with the • Chemicals, biologicals, and type of change in a protocol may seem workplace hazards, and does each radiologicals are well-organized, benign, it is not! Using sharps in a person routinely use appropriate properly stored, and labeled; laboratory that handles potentially controls, such as good lab work infectious materials signifi cantly practices, fume hoods, biosafety • Lab personnel wear appropriate increases the risk of occupational cabinets, and personal protective personal protective equipment (e.g., exposure. Generally, using sharps equipment, for these hazards? safety eyewear, gloves) and use during manipulation of human If you said yes, you are working in a appropriate controls; pathogens or potentially infectious safe lab! materials (for other than parenteral • Personnel receive basic training injection of animals) is not allowed Who Ensures on health and safety hazards and at NCI-Frederick, unless the IBC has That Your regulations, and are current with specifi cally reviewed and approved Lab Is Safe? re-training requirements; the procedure. Principal investigators, • Safety information is regularly lab managers, incorporated into group meetings and supervisors have the primary and is documented; and responsibility for ensuring that the laboratory and other work areas • Lab personnel regularly use EHS as Are You Prepared to Respond to under their supervision are safe a resource—EHS and lab managers a Biological Emergency? places to work. To make sure all interact frequently. laboratory personnel use safety Dealing with laboratory accidents procedures appropriate for the Biological Safety and the IBC should be an integral part of laboratory materials being handled, the workers Before initiating work with training. Are you prepared to respond must be made aware of the nature of pathogens, human-derived cells or effectively? Whether your exposure the material (e.g., infectious versus materials, or recombinant DNA, is contact with skin, mucosal inactivated pathogens), and the risks principal investigators are required membranes, or from a puncture wound associated with the material. The to register the work with the NCI- or cut, these sorts of events constitute active management of laboratory Frederick Institutional Biosafety signifi cant exposures and require practices remains a signifi cant role Committee (IBC). An integral part of immediate attention. Are you trained of supervisory staff and ensures safe the registration process ensures that to take the following steps? work practices. all laboratory personnel have been apprised of the risks associated with 1. Immediately initiate fi rst aid. Wash Secrets to Managing a Safe Lab their work. However, implementing contaminated skin for about 20 What are some secrets to managing safe work practices does not mean minutes with a povidone-iodine a safe laboratory? The primary secret that laboratory activities are simply scrub. Ensuring povidone-iodine is this: the lab manager or principal registered with the IBC. Since scrubs are readily available in investigator takes safety seriously. scientifi c research rapidly evolves, and your laboratory is a good start to Lab managers and supervisors should the scope of work within a laboratory immediate fi rst aid response to

The NCI-Frederick Poster 18 June 2006 Environment, Health, and Safety Program

a potential biological exposure, • What was the pedigree of the cell in the example cited earlier in this especially if a laboratory-associated line used to produce the virus? article? viral vector is involved (If a Finally, it is imperative that the mucosal membrane, such as your • Are there other hazardous materials principal investigator and/or those eye, is exposed, fl ush the membrane involved in the exposure? identifi ed in protocols be fully aware at an eyewash station for at least of their laboratory activities, be 15 minutes). Many factors are considered knowledgeable about the hazards when contemplating post-exposure involved with their lab’s work, and 2. Notify the laboratory manager or prophylaxis. When dealing with take responsibility for ensuring that supervisor as soon as feasible. retroviral vectors and/or live virus, lab personnel are trained in appropriate time is important. Although approved hazard mitigation measures and safe 3. Notify Occupational Health Services standard operating procedures and laboratory practices and procedures. (301-846-1096) about the incident approved IBC registration documents The best way to deal with a potential as soon as possible. If there is an may be in place, the only way exposure is to provide the necessary emergency after hours, call 911. EHS and the IBC can assure safe safety systems to ensure the exposure practices and procedures are being event is avoided altogether. Before you report to OHS, you should followed is through the diligence of You may already have summer answer several questions for yourself: the supervisor, mandatory training interns in your laboratory. Ensure for technicians (with verifi cation of that students as well as staff are • What infectious material was I their competency), and continuously knowledgeable about laboratory working with (bloodborne patho- monitoring performance. safety. Please use EHS as a resource! gens, viruses, viral vectors, etc.)? Recommendations to ensure safe lab If your lab does not have everything a practices may also include changing safe lab should, contact us. We will be • What was the infectious dose of the equipment and procedures. For delighted to help get your lab on track material I was manipulating? example, would a retractable needle to being safe. EHS can be reached at device have avoided the exposure 301-846-1451.

Safety Quiz: What’s Wrong with This Picture?

Can you fi nd at least 12 safety violations in the picture at right?

Answers on page 26

The NCI-Frederick Poster 19 June 2006 Environment, Health, and Safety Program

Fitness Challenge 2006 is dietary choices throughout the holidays. It’s never too late to join, or re- Heating Up! Check the Web site for details. join, this facility-wide effort. You must have an on-line account in NCI-Frederick’s Fitness Challenge Don’t Forget to Record order to have your individual and 2006 is heating up along with the Your Progress! organizational progress be counted. To seasons. In case you haven’t yet heard The only way to see your own create your personal account, log on to of this program, here are the basics: personal progress as well as add your the Web site for instructions, visit the the goals are that NCI-Frederick results to those of your directorate is to CHI in Building 549 between 12:00 collectively lose 1 TON (2,000 log on to the Challenge Web site: and 1:00 PM on Wednesdays, or call pounds) of body weight; walk, run, OHS at 301-846-1096 for additional or bike AROUND THE WORLD http://saic.ncifcrf.gov/fi tnesschallenge/ assistance or information. (~25,000 miles); and perform 1 YEAR (8,760 hours) of other fi tness Record your activities in the “Fitness How Does Your Group activities throughout 2006. All of the Tracker,” a feature that allows you Stack Up? organizations at NCI-Frederick are to view your personal progress and At press time, the Applied in competition with one another to compare it with all others in your own Developmental Directorate was claim the greatest success by the end directorate and the facility as a whole. leading the charge, with the most of the year. Got it? Good…so, let’s get You may also view the progress of all number of pounds lost per person. moving! directorates, so you can see how your These statistics are based on the data directorate stacks up against the others entered by participants, so don’t forget You Have Lots of Support (see table and fi gure below.) to record your results. Occupational Health Services (OHS) has partnered with the Scientifi c It’s Not Too Late to Participate Library’s Center for Health Information The staff at OHS are encouraged (CHI) to support this program. The CHI by the positive results this program offers a wide variety of diet, nutrition, continues to have, and we feel and fi tness links on its Web site (http:// fortunate to be able to play a part in www-library.ncifcrf.gov/chi.aspx) that helping our fellow NCI-Frederick can help employees develop a healthier employees develop and maintain a lifestyle. The CHI also offers individual positive lifestyle. Visit the Web site assistance with registering and tracking to keep informed of our upcoming your progress on the Fitness Challenge events, fi nd regular fi tness tips, and get Web site, every Wednesday from 12:00 healthy recipes! PM to 1:00 PM. OHS has also worked closely with Data Management Systems (DMS) to provide an interactive Web site (http:// We’re Getting There! saic.ncifcrf.gov/fi tnesschallenge/) to Here’s how we’re doing as a group. All information is based on data entered support the Challenge. The Fitness into the Fitness Tracker as of June 9. We look great in the “Other” category, but Tracker, health information, fi tness we need to crank it up in weight loss and biking, running, and walking. We’ve tips, “Find-A-Buddy,” recipes, and got less than six months left, so let’s go, NCI-Frederick – we can do this! links to useful Web sites are just some Average Percentage of the resources you’ll fi nd on this Activity Total Goal PerPerson Accomplished dynamic Web site. The monthly “Lunch and Learn” Pounds lost 4 512 2,000 26% programs provide information you Miles Biked 83 3,230 can use to meet your personal goals. Miles Run 39 1,815 Upcoming events will cover subjects Miles Walked 36 3,949 including fi tness tips for beginners, the Total, bike, run, walk 8,994 25,000 36% pros and cons of fad diets, how to get your family involved, and controlling Hours Other 60 5,427 8,760 62%

The NCI-Frederick Poster 20 June 2006 Student Intern Awards

High School Student “Removal of Estrogenic Compounds whom 4 were SIP students. Three Interns Win Awards in Dairy Waste Lagoons by Ferrate current-year SIP students winning at Science Fair (VI): Oxidation/Coagulation.” awards for microbiology were Sean Mr. Remsberg will be starting his McCann, First Place (mentors, Drs. Werner H. Kirsten Student Internship internship this summer with Dr. Lucy Anderson and Yih-Horng Shiao); Program (SIP) students came home Nadya Tarasova in the Structural Danielle Guiffre, Second Place with top honors from the 25th Biophysics Laboratory. First runner up (mentors, Drs. Robert Blumenthal and Annual Science and Engineering was Frederick High student Cortney Amy Jacobs); and Charles Zhu, Third Fair on March 25. This annual event Ragatz for her fi rst-place medicine Place (mentors, Drs. James McMahon is sponsored through a partnership and health/gerontology project, and Barbara Giomarelli). In addition, between Frederick County Public “B-Catenins Role in Drug Resistance.” Teddy Kamata (mentor, Dr. Ira Daar), Schools and the Frederick Jaycees. Ms. Ragatz is a current SIP student an incoming SIP student, won Second MedImmune provided funds for this whose mentor is Dr. Susan Mertins of Place in biochemistry. year’s fair. the Screening Technologies Branch. Please join The Poster staff in Out of about 120 participating Together, these students represented congratulating all the participants middle and high school students, the Frederick County at the International in the science fair for a job well grand prize was awarded to Jarrett Science and Engineering Fair in done, and all the SIP mentors for Remsberg of Middletown High for Indianapolis, Indiana, in May. encouraging these bright future his environmental science project, First-, second-, and third-place scientists! awards went to 12 participants, of

The March Poster Puzzler winners: Michael Malasky, Research Assistant, and Jami Troxler, Research Technician CORE Laboratory, pictured here with Paul Miller, Executive Editor of The Poster, in front of Building 538.

The NCI-Frederick Poster 21 June 2006 Outreach and Special Programs

Students Not Immune microscope so that the students to Science could study the cells. All the items served to emphasize her Recently, biologist Robin Winkler- point that “every class you have Pickett, Laboratory of Experimental is very important. Language Immunology, took on a class of sixth-, arts—I have to know how to seventh-, and eighth-grade special write; the calculators—we have education students with disabilities to know our math, so we can ranging from cerebral palsy, emotional graph our data; and even social disturbances, and speech and language studies classes help because we impairment to learning disabilities. work with people from all over Ms. Winkler-Pickett provided the the world,” she explained. students with a culminating activity on their study of the immune system, dovetailing her lesson “Ka-pow! Outta here!” into the class’s previous studies By the time the students looked at of human biological systems. the mouse cells, Ms. Winkler-Pickett “It was really great because had prepared them thoroughly, with they remembered things information geared to their level. A from the beginning of the natural teacher, Ms. Winkler-Pickett semester and shared personal led one student, reluctant to approach experiences,” she said. the microscope and the cancer cells, “I explained that sometimes through logical steps to understand the people are sick, perhaps idea of compatibility markers. with an infection, and take antibiotics, or they might have “These are mouse cells, and you’re cancer. When I asked, ‘Does anybody a human,” she said, reminding him know anybody who’s had cancer?’ one of her earlier statement about the young man, who had stayed very quiet body’s response to a foreign object: until then, raised his hand and said, “The body says, ‘Ka-pow! You’re ‘My grandmother.’ He knew how worn outta here!’ to the foreign object.” down she was from the treatments, and he understood what his grandmother “Now,” she asked him, “even if was going through.” these things get on you, they’re not human, so what’s your body going “Every class you have is very to do?” important.” The child answered, “Pow! Outta In addition to CDs and here!” After that, he was eager to other teaching materials examine the cancer cells. that Barbara Birnman’s Elementary Outreach staff “He just amazed me that he got provided, Ms. Winkler-Pickett that concept of compatibility,” Ms. brought in some living mouse Winkler-Pickett said. cancer cells in a fl ask, pictures of the mice from which the cells had been taken, a graph, a calculator, one of her published articles, and a big

The NCI-Frederick Poster 22 June 2006 Outreach and Special Programs

Mrs. Winkler-Pickett noted from other classes would come in that as she used scientifi c to see the pictures, too, and my kids terminology with the explained what they had learned.” For students, she defi ned the example, Ms. Whipp’s students could terms. If she forgot, Brenda explain that the mice with no hair had Whipp, the special education no immune system and were more teacher, would prompt her by susceptible to disease, “and they could saying, “Now, Ms. Pickett, relate the mouse cells to human cells what is that?” that make up our own tissue.” Noting that Mrs. Winkler- Pickett encouraged the A Huge Heart for Science students to ask lots of Ms. Winkler-Pickett pointed out that questions, Ms. Whipp the students “are getting concepts that said, “This was one of the other people just don’t get. I certainly culminating activities at the end of can see them someday in a support the science unit. And so, it was a big function or mechanism, at NCI- deal for them, that they would have a Frederick. These kids have a huge real scientist come and talk with us. heart and enthusiasm for science.” And the kids were very curious. They knew who Ms. Pickett was, because I now have some students telling they had met her in December, and her me that they want to be son, Adam, is in the class, but to see in a science fi eld. her in her professional role was a bit different.” Ms. Whipp agreed. “They’re very inquisitive. In special ed, one of No One’s Ever too Old the things that you’re constantly to Play with Dry Ice asking your students is, ‘Where Mrs. Winkler-Pickett chuckled do you want to go from here?’ I now as she added, “I had two have some students telling me that instructions from Adam, which they want to be in a science fi eld. That were to talk about ‘cloning’ lesson was a wonderful eye-opener for mice, which is kind of his term, them.” and to bring dry ice. No matter how old you are, there is always going to be a fascination with dry ice. When we pulled out the dry ice, water, and the liquid soap, and made dry ice bubbles, they were excited. I had hands all over the place!” Perhaps one of the best outcomes of the lesson was that these students were so excited about what they’d learned, they wanted to discuss their new knowledge with relative strangers, teachers, and students from other classes. Ms. Whipp explained, “Kids

The NCI-Frederick Poster 23 June 2006 Campus Improvement Committee

Campus Improvement Committee Stays Busy Campus Is Improved with Flower Power

Many of the fl owers you see in glorious bloom around the campus are here thanks to the efforts of our industrious Campus Improvement Committee. In March the committee organized about 20 volunteers to plant 400 geranium seedlings in small pots. These were cared for in one of the USDA greenhouses, and were transplanted in May throughout the facility. Volunteers also came together to plant a few thousand seeds of colorful annuals, including four-o-clocks, pansies, zinnias, marigolds, and rudibekias, Signs of Improvement which were also transplanted throughout the facility. Other signs of Campus Improvement Committee activity are, literally, signs. New building number signs were installed throughout the spring and provide easy identifi cation of all buildings. More important, this project Our Own Wye Oaks Take Root is part of a larger, identity plan. “Our intent is to identify our buildings as If you haven’t seen them yet, you belonging to NCI and to get a couple should stop by to pay your respects to of hundred more NCI logos on display the two Wye Oak seedlings that the on our campus,” Ken Michaels, committee planted last fall on the east chairman of the Signage Committee, and west sides of Building 560. These recently explained. The new signs are the offspring of the mighty Wye have a clean, crisp look and, along Oak, which stood in quiet majesty for with the banners, instantly identify over 400 years in the village of Wye NCI-Frederick to personnel and Mills, Maryland. The oldest and the visitors alike. largest white oak tree in the nation, the Wye Oak was declared the living The Campus Improvement symbol of Maryland’s state tree in Committee is always interested in 1939. A severe storm brought the tree getting community input. If you have down in June 2002, but, thanks to the an idea of how we can improve the Campus Improvement Committee, we NCI-Frederick campus, we would like can share in its legacy. The seedlings to hear from you. Submit your ideas have wintered well, and appear to be or comments on-line through our Web making themselves at home on our site: http://web.ncifcrf.gov/campus/ campus. committees/campus.asp.

The NCI-Frederick Poster 24 June 2006 Summer Fun

Summer Fun Right Here in Register for swimming lessons, Bowling Parties: Can be customized Our Own Backyard! Session IV: July 24-28, Session begins to your next event. Call 301-619-2816 July 31. For information, call the for details. The Morale, Welfare, and Recreation Outdoor Pool, 301-619-2368. Paint Ball Course: Now open, Directorate (MWR) of Fort Detrick Battle of the Beast: August 5. Old Area B. This is one of the best paint offers programs and services to time bull riding competition at the ball courses in the area, with great benefi t the health and well-being of all W.J. Bar Ranch. Call 301-619-2957 prices and fun for all. Groups are personnel on base. As employees of for details. welcome by advance reservation. For NCI-Frederick or its contractors, we Nallin Farm Reservations details, call 301-619-0247. may take advantage of these services, The Nallin Farm Recreational Area Swimming: Buy your pool pass now! too. Some of the activities are listed is complete with three shelters, Pool pass entitles holder unlimited here, but a complete listing of services a grill pavilion, horseshoe pits, a use of both indoor and outdoor pools: and activities may be found on the stocked trout pond, playground, and The outdoor pool, located next to the MWR Web site, http://www.detrick. bathrooms. Groups must reserve tennis courts, is open from Memorial army.mil/wellbeing. the area seven days in advance. For Day through Labor Day. The indoor details, contact the Directorate of pool, located in the Odom Fitness Enjoy movies under the stars! Morale, Welfare, and Recreation, 301- Center, is open year round. Telephone: July 7, Blue and Gray Field, 8:30 p.m. 619-2711 from 10:00 a.m.–4:00 p.m., 301-619-2368 for more information. Check the Web site for details. Monday through Friday. V-Mail Delivery: Send an old Community Yard Sale: July 15, Blue Community Fun Fest: September 9. fashion V-Mail to your special military and Gray Field, 8:00 a.m.–2:00 p.m. Watch for advertisement. This is one person (in Landstuhl Army Regional Make money while you clean out of Fort Detrick’s most popular family Medical Center in Germany, or in Iraq those closets, garages, and storerooms! events of the year. or Afghanistan). Call 301-619-7510 $10.00 for two tables, bring your own for details. chairs. For more information, call 301-619-3237.

PALS

From head to toe, there were giggles galore at the Play and Learning Station (PALS) on “Silly Hat” day, May 9, and “Silly Sock” day, May 11.

The NCI-Frederick Poster 25 June 2006 NCI-Frederick Central Repository: Fisher BioServices

Update on the Information to date, including a description of the and a percentage of visual checks. Technology Initiative new system, timeline for transition, Some data conversion issues require and data conversion issues; discussion interface with repository users for Response from user surveys over of current Web forms and future Web resolution. This process has begun. the past several years has indicated access; and open discussion. The Many investigators have heard that the top two repository operational workshop provided an opportunity for from our staff and responded to our needs of the scientifi c community are: users to share their ideas and concerns. inquiries. 1) convenient real-time electronic Workshop evaluations provided access to sample data, and 2) the valuable information on which future New Fields Required ability to interface electronically with discussions will be based. Copies of Additionally, the new system has the repository for specimen input the presentations can be found on the several required fi elds which are not and withdrawal. We heard you. After Central Repository Web site, http:// available in the current system (see three years of evaluation, planning, www.ncifcrf.gov/repository/cr/info_ slide 33 of the IT Details presentation programming, and testing, the roll-out tech.asp. found on the Web site). Of importance of the new system is scheduled for are study name and study ID. Prior October 1, 2006. Over Two Million Records to transition, users will have the To Be Transferred opportunity to indicate their preferences Workshop Held in January to New System for the research study names and On January 26, 2006, Fisher Transitions of this magnitude study IDs. Additionally, users will BioServices hosted a workshop involve gathering of requirements, be able to reserve BSI Label IDs for to share with repository users the programming, testing, and data specimens which will be submitted to background, plans, and timeline for conversion. Of these four, data the repository. The Central Repository transition from the current Central conversion has the most unknowns Web site will be updated soon to Repository inventory system to the and can be the most problematic. The allow repository users to indicate their BioSpecimen Inventory – II (BSI- current system has over two million preferences for these fi elds. Fisher II) system, produced by Information records which will be converted BioServices staff encourages you Management Services. The one-day and transferred to BSI-II. Fisher to visit the site regularly. Updates workshop was well attended by a good BioServices has analyzed current regarding the timeline, information cross-section of people involved in data using statistical analysis and about training, frequently asked one way or another with repository 20 unique fi eld identifi ers. We have questions, and opportunities for input operations. also developed a variety of quality and feedback are available on the Web The workshop was presented in four control checks to ensure accuracy site listed earlier. parts: background, history, and goals upon transition. These include of the initiative; update on progress 100% automated data comparison

Answers to Safety Quiz on page 19:

1. The researcher is transferring 5. The researcher has no eye 10. Sharps (needles) are improperly material by mouth pipetting. protection. stored and have not been placed 2. There’s a mug—and, 6. The gas cylinder should be on the in a sharps container. presumably, a drink—on the lab fl oor, and secured. 11. Radioactive isotope vials counter. 7. The hot plate is on a combustible are incorrectly stored on the 3. The researcher has not fully surface (the Sigma catalog). benchtop and are not labeled. fastened his lab coat. 8. The waste container is not closed. 12. The bench paper is glossy 4. The researcher is not wearing 9. The waste container is not labeled/ side up. gloves. tagged with contents.

The NCI-Frederick Poster 26 June 2006 SAIC-Frederick, Inc.

and product development, the alliance Industry Organization (BIO), in helps employers develop the tools Chicago. As part of the Maryland and education necessary to become Expo booth, the NCI-Frederick recognized as excellent places to work. contingent distributed information and answered questions about the SAIC-Frederick, Inc., Signs WFO/EA (Work for Others/Economy Agreement with FITCI Act) programs as they apply to NCI- Partly as an outgrowth of our Frederick’s services and expertise now participation in the Work for Others available to private industry and other program, SAIC-Frederick, Inc., government agencies. SAIC-Frederick, Inc. Earns has signed an agreement with the Speakers at the convention included Fourth Annual Frederick Innovative Technology former President Bill Clinton, Health Alliance for Workplace Center, Inc. (FITCI), a technology and Human Services Secretary business “incubator” located at Hood Michael O. Leavitt, actor Bernie Mac, Excellence Award College. Under the agreement, client and award-winning journalist Neil For the fourth consecutive year, companies at FITCI may be able to Cavuto. Nearly 20,000 people from 62 SAIC-Frederick, Inc., has received the obtain special or unique services from countries attended. “Seal of Approval” from the Alliance NCI-Frederick, especially those related BIO represents more than 1,100 for Workplace to the laboratories within the Research biotechnology companies, academic Excellence Technology and Biopharmaceutical institutions, state biotechnology (formerly the Development Programs, which may centers and related organizations MD Work~Life not be available elsewhere. Each across the United States and 31 other Alliance). The request will be evaluated, with our nations. BIO members are involved annual award response dependent upon available in the research and development of recognizes capacity to provide the work. Each healthcare, agricultural, industrial, and visionary action would be subject to NCI review environmental biotechnology products. employers that view workplace and approval. excellence as a strategic business FITCI, a non-profi t organization imperative, creating workplaces where founded in 2004, provides local employees can achieve success in entrepreneurs with fully equipped all aspects of their professional and offi ce space, as well as the shared use personal lives, while enhancing the of various Hood facilities and support business bottom line. equipment. Commercial and wet Publication Awards Dr. Larry Arthur, president and laboratory spaces are also available. Two products of Scientifi c CEO of SAIC-Frederick, Inc., stated In addition, FITCI staff provides its Publications, Graphics & Media that “Receipt of this recognition for customers with business plan reviews; recently were recognized with four years in a row demonstrates our consulting services; a mentoring an Award of Distinction and an continued focus and commitment program; networking, marketing, and Honorable Mention through the to our employees and their need public relations assistance; periodic annual Communicator competition, to balance work and personal life. seminars on business topics; and an international awards competition We recognize that our employees’ funding assistance. that recognizes outstanding work in dedication to excellence in all aspects all specialties in the communications of cancer and AIDS research is the NCI-Frederick Represented fi eld. This year 5,214 entries were foundation of our success and that our at BIO 2006 in Chicago judged in the Print Media competition. people are our greatest resource.” Dr. Carl Garland, Research The Award of Distinction is awarded The Alliance for Workplace Technology Program; Mary Lou for projects that are judged to “exceed Excellence is a 501(c)3 nonprofi t Siegle, Human Resources; and industry standards” in communicating organization funded by Montgomery Dr. Bruce Crise, Gene Expression a message or idea. About 18% of the County, Maryland, and corporate Laboratory, RTP, recently attended BIO entries won this award. Honorable sponsors. Through its key strategies 2006, the 14th annual international Mention certifi cates are granted to of training, management consulting convention of the Biotechnology continued on page 28 The NCI-Frederick Poster 27 June 2006 Data Management Services (DMS)

Data Management both administrative and scientifi c Requests for service can also be Services: Computers and programming needs, as well as Web placed via the C&SS Web site (http:// Statistical Support design and development services. css.ncifcrf.gov/helpdesk) at any time. Visit the C&SS Web site at Although perhaps most widely http://css.ncifcrf.gov or call Site-Licensed Software known for our Microcomputer Support 301-846-1060 for information about Available from the Helpdesk! and Web Development services, C&SS custom development services available C&SS, in conjunction with the NCI, also offers many other services to the from C&SS. has worked to secure site licenses for NCI-Frederick community. In this many of the programs in broad use at issue of The Poster we highlight some Technology Advocacy and NCI-Frederick. To view the growing of these other services. Consultation list of software available from the As NCI-Frederick’s information Helpdesk, visit the C&SS Web site Statistical Consultation technology experts, C&SS continually at: http://css.ncifcrf.gov/helpdesk/ The Statistical Consultation explores and evaluates new software.asp or contact the Computer group provides a wide array of technologies that could benefi t the Services Helpdesk to borrow the mathematical and statistical consulting user community and further NCI- software or request installation services to the NCI-Frederick Frederick’s mission. C&SS staff would assistance. scientifi c community. The director be happy to meet with you to discuss and consulting statisticians work your specifi c technology needs. in collaboration with principal Contacting C&SS investigators through all facets of the Computer Software Training Computer Services Helpdesk scientifi c process: from development Watch for postings and e-mail Web: http://css.ncifcrf.gov/helpdesk and formulation of research and notifi cations for the summer schedule of E-mail: [email protected] statistical hypotheses through design software training classes. Phone: 301-846-5115 of experiments and statistical analyses, Please see the Computer Software preparation of technical reports and Training Web site at http://css.ncifcrf. Hours of Operation: modern graphics, to preparation of gov/training for information or to 8:00 a.m.–5:00 p.m., formal scientifi c documents and register for classes. Monday through Friday publications in peer-reviewed journals. The Computer Services Helpdesk provides the NCI-Frederick NCI-Frederick Webmasters Custom Software Development community with a single point of Phone: 301-846-6700 Our team of analysts and developers contact for computer assistance, E-mail: [email protected] employs the most modern methods information, service, and support. The and tools to create custom software Helpdesk is staffed from 8:00 a.m. to Other Inquiries solutions to meet the unique needs 5:00 p.m., Monday through Friday, Phone: 301-846-1060 and requirements of NCI-Frederick. excluding NCI-Frederick holidays. Our staff can assist you with continued from page 27 Rogers. The design and production summary (which also won an Award those entries that “meet the high team included Allen Kane, Kathy of Distinction in this competition last standards of the industry.” Green, Nancy Parrish, and Maritta year). Connie Suders led the content Grau. update and development, assisted Honorable Mention: by Maritta Grau and Nancy Parrish; Advanced Technologies. The four- Award of Distinction: Kathy Green and Allen Kane provided color brochure and accompanying Executive Summary, 2005 Annual graphic re-design. family of eight inserts described Report. This 16-page, four-color SPGM has won 11 awards and nine services available through the publication won in the “Other— honorable mentions in various juried Economy Act/Work for Others Executive Summary” category. competitions during the past two provisions and were produced for Published in autumn 2005, the project years. Carmen Clark, Carl Garland and Keith updated the previous executive

The NCI-Frederick Poster 28 June 2006 Wilson Information Services Corporation (WISCO)

Librarian’s Picks Something New in the Center for Health Recycling has many Information (CHI) benefi ts, some not even related to the environment. Do you eat lunch in the Café? Do The Library holds its Book you attend seminars in the Conference & Media Swap every Center? If so, why not drop by the October, where you can take Library to see how you are doing with advantage of someone else’s weight control and heart healthiness? bibliophilia, for free! Here For your convenience, a self-service is a sample of titles our most recent Song of Solomon by Toni Morrison blood pressure monitoring system has participants had to choose from: A powerful, sensual, and poetic been installed, as well as a scale. It’s exploration of four generations of quick, it’s easy, and it’s private (Rex Angels and Demons by Dan Brown a family. Readers will discover a and Rosie are still in residence, but An explosive international thriller century’s worth of secrets, ghosts, they are sworn to secrecy). So why not from the author of The Da Vinci Code. and troubles. give it a try? Blood Work by Michael Connelly A Virtuous Woman by Kaye Gibbons A spine-tingling manhunt guaranteed Author Gibbons creates a multi- Classes at the to boost the blood pressure. layered and indelibly convincing Scientifi c Library The Devil Wears Prada by portrait of two seemingly ill-matched Orientation Lauren Weisberger people who somehow miraculously July 19, August 9, September 13 A delightfully dishy novel about the make a marriage. 2:30 p.m. all-time most impossible boss in the history of impossible bosses. Be sure to leave room in your Making Sense of DNA The Face by Dean Koontz schedule for next year’s event. Who and Protein Sequences The Face will not leave Koontz fans knows, some of these titles may be on July 6, 12:30–3:00 p.m. disappointed with this bestselling the shelves again. novel of suspense and adventure. Entrez Gene Quick Start King of Torts by John Grisham July 26, 12:30–3:00 p.m. Grisham’s most unusual legal thriller yet. A compelling tale of corruption Unmasking Genes in of the mind and soul. the Human Genome Nights in Rodanthe by Nicolas Sparks August 22, 12:30–3:00 p.m. A timeless love story of two people who meet by chance in the small North Please call 301-846-5840 for more Carolina coastal town of Rodanthe. information. The Pilot’s Wife by Anita Shreve Shreve does a wonderful job weaving anguish, love, mystery, and resolution in The Pilot’s Wife. Portrait of a Lady by Henry James A story of intense poignancy, Henry James’ tale of love and betrayal still resonates with modern audiences. Shepherds Abiding by Jan Karon Scientifi c Library staff were The eighth novel in the bestselling busy as bees at their Spring Mitford Years series is a meditation Research Festival booth. on the best of all presents—the gift of one’s heart.

The NCI-Frederick Poster 29 June 2006 New Faces at NCI-Frederick

NCI-Frederick Welcomes New Staff Ninety-one people joined our Facility in January, February, and March 2006.

NCI-Frederick welcomes… Charles Rivers Laboratories Christopher Badorrek Yihui Gong welcomes… Suhwan Chang Said El Kassouli Judy Downs Ken Fujii Anna Ilinskaya Jordan Irvin Tinoush Moulaei Charles Early, Jr. Ping Sun Andreas Sundgren

SAIC-Frederick, Inc., welcomes… Selam Abebe David Circle Havic Muchapondwa Jeffrey Appel Jeffrey Clogston Irene Mueller Adil Asheer Talisa Creavalle Aurachalee Musikabhumma Rolanda Bailey Heather Cronise-Santis Mary Nichols Paul Biser Charles Davis Tiffany Nikirk David Blythers Norma Diaz-Mayoral Amelie Ntsama Brian Boland Timothy Dotson Michael Osmun William Bowbliss Kimberly Dreyer Demetria Parks Mikhail Bubunenko Charles Early, Jr. Traci Parlett Susan Chinkuli-Mccready Shaun Einolf Marianne Rivera-Silva Erica Emeigh Maria Romero-Izquierdo Andreas Sundgren Jessica Etzler Kathleen Rosenfeld Elizabeth Ferrell Vali Sevastita Mary Simpson Judy Downs Tamika George Yihui Gong Mark Slatcoff Lisa Gregory Thomas Smith, Jr. Linda Griffi th Drina Sta Iglesia Viktoriya Grinberg Sara Stallings Shannon Gupta Jacqueline Stoddard Tammy Harding Christopher Stutt Taquita Haygood Cung Thang Wayne Helm Martha Torres Herbert Higson Shirin Treadwell Paula Jacobs-Brooks Jennifer Troyer Christopher Badorrek Jiuping Ji Stephanie Wahl Ming Ji Thaung Waih-Kam Said El Kassouli Man-Shiow Jiang Edward Waterfi eld Emmanuel Jones Jennifer Wilder Awn Kam Kathleen Williams Rebecca Keyser Leon Williams Matthew Lamb Catherine Wilt Jean Lapadula Danny Wolff Julia Lee Jiwen Zheng Lavonia Logan April Lopez Heather Marshall Shane May Dawn Mcdowelle

The NCI-Frederick Poster 30 June 2006 Café NCI-Frederick

Café NCI-Frederick, Catering Services Available Building 549 Looking for food to go along with Great selections at great prices! your next meeting or offi ce party? The Café NCI-Frederick can help! The Café NCI-Frederick is open to Catering packages are available all personnel who work on the NCI- with a large selection of items to Frederick-Fort Detrick campus. If you’re choose from, at a reasonable cost. used to getting your breakfast or lunch Call 301-846-1750 to make your off campus, you know how expensive arrangements. and inconvenient it can get. The Café NCI-Frederick is your solution! The Café is open Monday through Friday, 7:00 a.m.–10:00 a.m. for Breakfast and Lunch Just breakfast, and 11:00 a.m.–2:00 p.m. Got Easier for lunch. If you would like to make Stop by before work to choose from a a suggestion to our menu selection, plentiful breakfast selection, including or if you have any questions, please eggs to order, breakfast sandwiches, call 301-846-1750. omelets, pancakes, sausage, biscuits and gravy, home fries, and more. You can check out the wide array of lunch continued from page 17 choices from the menu on-line at Students http://web.ncifcrf.gov/campus/cafe/, or Kimberly Shafer-Weaver — Laboratory of Cell-Mediated Immunity, CSP (Immunology) Modulating Tolerance of Tumor-Specifi c T Cells in a Murine Model of Prostate Cancer pick up a menu in the Café. Sean McCann — Laboratory of Comparative Carcinogenesis (Molecular Biology) This summer, the Café will feature a Allele-Specifi c Methylation Changes in the Non-Imprinting 45S rRNA Promoter Regions “grab-n-go” section of pre-made salads after Environmental Exposure and sandwiches, fresh fruit daily, and Technicians SPC Gibson, Christopher — DSD, USAMRIID (Biodefense) our popular, cold strawberry-and- Development of a Protocol for Reusing the CombiMatrix Hybridization Platform melon soup. Or, if you’re in the mood Alma Arnold — Image Analysis Laboratory (Cancer Biology) for a good salad, there are 21 items to Measuring Migration Potential of Astrocytes in Three Dimensions choose from in the salad bar, including Carrie Saucedo — Molecular Targets Development Group (Drug Development and Delivery) Solabellin, a Novel Anti-HIV Protein Isolated from Solanum umbellatum. 4 types of lettuce. Vickie Marshall — AIDS Vaccine Program (Genetics and Epidemiology) Phylogenetic Characterization of Virally-encoded MicroRNA’s in the Kaposi’s Sarcoma– Associated Herpesvirus (KSHV) Jeff Subleski — Laboratory of Experimental Immunology (Immunology) IL-18 + IL-12 Induces Antimetastatic Activity in the Liver by Modulating NK and NKT Cells Theodore Luck — Laboratory of Cell-Mediated Immunity, CSP (Immunology) Sensitivity Training: Optimizing the Granzyme B and Perforin ELISPOT Assays Danielle Fink — Neutrophil Monitoring Laboratory (Immunology) Nonspecifi c Deposition of IL-8 by Human Neutrophils during Chemotaxis In Vitro Krishna Moody — Division of Bacteriology, USAMRIID (Infectious Pathogens) Biochemical Analysis of Bacillus anthracis Spores from Fully Virulent Strains Wei Gao — Laboratory of Immunopathogenesis and Bioinformatics (Informatics) SNPit: A Genetic Software Analysis Tool for High Density Genotyping Association Studies Craig Cavin — USDA-ARS-Foreign Disease Weed Science Research Unit (NewTechnology) Host Range Determination of Colletotrichum gloeosporioides from Russian Thistle Jennifer Meyers — USAMRIID (Vaccines and Gene Therapy) CpG ODNs Enhance the Effi cacy of the Yersinia pestis F1-V Vaccine Assessed by Parenteral and Aerosol Challenges in a BALB/c Murine Model for Plague. Wendell Miley — AIDS Vaccine Program (Virology) Measurement of HCV Genotype 1-6 Viral Loads by Real-time Quantitative RT-PCR

The NCI-Frederick Poster 31 June 2006

The Poster Staff Employment Opportunities Executive Editor Paul Miller Associate Editor Please contact the individual contractor’s human resources Ken Michaels representatives or go to the contractor’s Web site for Managing Editor up-to-date, detailed information about jobs or research Maritta Grau and training opportunities and requirements. Co-Editor Nancy Parrish Production Editor Charles River Laboratories Kathy Green http://www.criver.com Lead Designer Tammy Schroyer Data Management Services Photography Editors http://css.ncifcrf.gov/about/dms.htm Jonathan Summers Marti Welch National Cancer Institute at Frederick Contributing Editors http://www.training.nih.gov/postdoctoral Administrative Resource Center Debbie Dixon SAIC-Frederick, Inc. Judi Carter http://saic.ncifcrf.gov Tanya Sappington www.saic.com Charles River Laboratories Cliff Hubbard Wilson Information Services Corporation Community Outreach http://www-library.ncifcrf.gov Barbara Birnman Julie Hartman Data Management Services Look for the Following Events Around Campus: Stephanie Sheppard Facilities Maintenance and Poster Puzzler—Entry Deadline: July 28, 2006 Engineering Deborah Dobbe Take Your Child To Work Day—July 12, 2006 Environment, Health, and Safety Fitness Challenge Learning Lunches—second Thursday of each month. Program Check the Web site for details: http://saic.ncifcrf.gov/fi tnesschallenge/ Alberta Peugeot Frederick Employee Diversity Team Farmers’ Market—Every Tuesday, 11:30 a.m.–1:30 p.m. (or sellout) Scott Keimig Fisher BioServices Kathleen Groover Summer Adventure Masterpieces Children’s Art Fair—July 25, 2006 Patricia Hindes Bake sale, lemonade stand, and silent auction to benefi t the NCI-Frederick SAIC-Frederick, Inc. Play and Learning Station (PALS) Child Care Center. Dave Bufter Science Today Bldg. 549 Café Conference Room, 11:00 a.m.–12:30 p.m. Paul Nisson Wilson Information Services Corporation Sue Wilson Robin Meckley Reminder: When you have a change in staff, such as new staff, a promotion, retirement, loss of staff, be sure to change the information on the NCI-Frederick database. You can do this online by Published four times a year by Scientifi c logging on to http://web.ncifcrf.gov/campus/phonebook/, or by contacting your human resources Publications, Graphics & Media for the National Cancer Institute at Frederick, representative. For more information, you may refer to the inside front cover of the NCI-Frederick Frederick, MD 21702. Telephone & Services Directory. http://web.ncifcrf.gov/ThePoster Comments or suggestions for The Poster may be directed to http://web.ncifcrf.gov/ThePoster

The National Cancer Institute at Frederick

Frederick, MD 21702-1201