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2018 Spring Elective 1. NEURAL! 15. KIDNEY! ! 16. BLADDER! 29. THYROID/! 2. HEART! 17. TESTIS! Parathyroid! / vessels! 18. PROSTATE! 30. ADRENAL! 3. ! 19. UTERUS! 31. PITUITARY! ! 20. OVARY! ---Eyes! 4. LIVER! 21. BREAST! ---Sinuses! 5. PANCREAS! 22. PLACENTA! 6. ! 7. STOMACH! 24. SKIN! 8. SMALL INTESTINE! 25. 9. COLON! 26. SMOOTH MUSCLE, , ADIPOSE! 10. SPLEEN! 11. TONSIL! 27. CARTILAGE! TUMORS! 12. ! 28. BONE! Assess for 13. LYMPH NODES! metastasis! 14. ! 1. Plan to examine blood and serum for abnormalities before planning histopathology studies

2.Looking at multiple tissues, from multiple animals is important to help explain abnormalities in one , in one animal. The organs do communicate with each other in vivo !!!

3. Plan to examine animals after they have been back-crossed into at least ~8 generations, to avoid artefacts

4. Plan to examine tissues from an initial set of 24 animals: --6 male and 6 female littermate; --6 male and 6 female gene altered

Squamous epithelium-- postive Cuboidal epithelium- around sweat glands --keratin positive (trichrome stain will help see this better) Sebaceous glands --vimentin Blood vessels--CD31 bundles Innate immune cells--CD45 etc

Keratinizing squamous epithelium of epidermis of skin

Dermis with sweat glands and sebaceous glands and hair follicles

Human : sweat and sebaceous glands Sweat glands: • Eccrine glands are found in all skin, with highest density in palms and soles. • Involved in Thermoregulation • Apocrine glands Sebaceous glands: • Produce oily sebum • Found on all parts of the skin except palms of hands and soles of feet in humans

Mice have sweat glands only on paws Sebaceous gland surrounding the base of a hair follicle and cervix has no keratin layer, no sweat glands, no hair follicles, no sebaceous glands etc. Melanocytes comprise from 5% to 10% of the cells in the basal layer of epidermis. Although their size can vary, melanocytes are typically 7 µm in length. The difference in skin color between lightly and darkly pigmented individuals is due not to the number (quantity) of melanocytes in their skin, but to the melanocytes' level of activity (quantity and relative amounts of eumelanin and pheomelanin). This process is under hormonal control, including the MSH and ACTH peptides that are produced from the precursor proopiomelanocortin. Albinos lack an enzyme called tyrosinase. Tyrosinase is required for melanocytes to produce melanin from the amino acid tyrosine.[8]

Mouse skin epidermis is usually very thin

Mouse skin and cartilage

INFLAMMATION AND REPAIR Is a protective response, where the goal is to rid the body of the initial cause of injury and the consequences ACUTE: relatively short duration. There is an alteration of blood vesels such that there is an exudation of fluid and plasma , with an emigration of leukocytes, predominantly neutrophils, into the focus of injury. CHRONIC: is of longer duration and is associated with the accumulation of and macrophages and allowing the repair process to occur, using angiogenesis and/ or . An example of abnormally thickened mouse skin epidermis as a result of inflammation An example of Trichrome stains being used to quatify scarring in dermal tissue Sections of skin and gut are usually good controls for TUNEL assays for apoptosis CELL DEATH: necrosis (occurs from the progressive degradative action of enzymes on the lethally injured cells) apoptosis: -programmed destruction of cells during embryogenesis -hormone dependent involution in the adult - cell deletion in proliferating cell populations, immune cells, tumors, etc.

Normal skin

Squamous carcinoma Carcinoma with mitoses

Transitional epithelium of the bladder epithelium: PseudoStratified Columnar NOT Stratified Squamous!

During pregnancy, there is rapid growth of the terminal ducts of the glandwith hyperplasia of the epithelium. In the second half of pregnancy there is an increase on size of the parenchymal cells and the alveoli are distended with secretions Normal cells ! !!!Carcinoma cells with ! from smear of cervix ! !altered nuclear: cytoplasmic ratio! !!!!

Markers of Differentiation Smooth Muscle Actin (SMA)

Normal Hyperplasia Tumor Identify organ/ lesion! Classify lesion: well-defined margins?! H&E of the carcinoma in a human breast Prognostic Markers in Human Breast Robbins and Kumar textbook of Pathology description of the process of malignant progression and metastasis! Identify organ/ lesion! Metastasis to Classify lesion: well-defined Brain margins?! Location of the mammary glands in the mouse! The mammary glands are greatly modified and enlarged sweat glands. The ducts and acini (i.e. the gland parenchyma) are formed mainly from cuboidal epithelial cells, with myoepithelial cells present to aid secretion. The contains elastic fibres and adipose tissue. Connective tissue lamellae divide the parenchyma into lobules. ! ! In the inactive mammary gland, the interstitial spaces between lobes and ducts are filled mainly with fat. There are a few alveoli present compared to those in the lactating gland, and the ducts and lobes of the gland are smaller than when the gland is in its active phase. ! ! During early pregnancy, the epithelial cells of the glandular tissue proliferate rapidly to form the buds which eventually enlarge to form the alveoli. As pregnancy develops, the fat and connective tissue between lobes and ducts is replaced by secretory tissue.

http://137.222.110.150/calnet/mammary1/page2.htm#section2! http://tgmouse.compmed.ucdavis.edu/SRW2/13SRhm.htm Rodent Mammary Gland Mouse mammary gland stages seen as a whole mount preparation With an example of a paraffin section H&E! Defective branching of alveolar mammary glands KO Wt What are the cell / tissue types you will see in breast tissue?

• Cuboidal cells--keratin positive • • Blood vessels • Fibroblasts • Nerve fibers • Innate immune cells

And in mouse mammary glands: #4 has a distinctive surrounded by breast ducts and lobules Non-epithelial supporting tissues:

Connective tissue:

• Endothelial cells and blood vessels • Collagen, fibroblasts important stromal elements • Elastic tissue • Reticular tissue • Adipose tissue • Bone • Cartilage • Muscle: • Skeletal—voluntary, striated, parallel fibers, eccentric nuclei • Cardiac—involuntary, striated, central nuclei • Smooth muscle—involuntary, central nuclei Bone has to be decalcified Bone and before it can be examined using the usual histochemical methods

REMOVE AS MUCH MUSCLE as possible

Fix in Cal-Ex II –Fisher Cat. No. CS511-1D (buffered formalin and

but do not let them be exposed to this for more than 3 days

Fix and then decalcify in EDTA if immunostains are to be done Decalcification solutions HCl; Formalin+ HCl; EDTA only- for slow decalcification for IHC BONE and CARTILAGE! ! BONE has to be decalcified before histologic examination! !Although there are orthopedic labs that use specialized !! !microtomes to section through undecalcified bone ! !to obtain information! CARTILAGE: ! --fibro cartilage, resembles dense connective tissue, is found in intervertebral discs, the pubic , in the attachment of some , and between the dense connective tissue of and capsules! --hyaline cartliage, consists of cartilage matrix and embedded in cartilage cavities called the lacunae! ---- in locations where support with flexibility is required as in the , of the ear etc.! Cartilage is a type of dense connective tissue . It is composed of cells called chondrocytes which are dispersed in a firm gel-like , called the matrix.

Cartilage is avascular (contains no blood vessels) and nutrients are diffused through the matrix. The main purpose of cartilage is to provide a framework upon which bone deposition could begin.

Another important purpose of cartilage is to provide smooth surfaces for the movement of articulating .

Hyaline Cartilage is the most abundant type of cartilage.

Elastic cartilage

Fibrocartilage is the most abundant type of cartilage.

The name hyaline is derived from the Greek word hyalos, meaning glass.

This refers to the translucent matrix or ground substance, that is made predominantly of type II collagen.

Hyaline cartilage is found lining bones in joints (articular cartilage) .

It is also present inside bones, serving as a center of or bone growth. In addition, hyaline cartilage forms the embryonic . Chondrocytes develop in the ). As they mature, they are moved deeper into the cartilage. There they actually secrete the matrix that traps them. Isogenous groups (small nests of chrondrocytes) result from repeated cell division.

Elastic cartilage is found in the pinna of the ear and several tubes, such as the walls of the auditory and eustachian canals and .

Elastic cartilage is similar to hyaline cartilage but contains elastic bundles ( ) scattered throughout the matrix.

This provides a tissue which is stiff yet elastic.

Fibrocartilage

is a specialized type of cartilage found in areas requiring tough support or great tensile strength, such as between intervertebral disks, the pubic and other symphyses ,

and at sites connecting tendons or to bones . . A tissue covering the bone that brings blood and lymph vessels, as well as nerves, to it ! *Compact bone (also known as cortical bone). Dense deposits of minerals - chiefly calcium phosphate - and collagen. These are arranged in concentric circles around a central through which blood and lymph vessels as well as nerves pass. ! *Spongy bone (also known as trabecular or cancellous bone). The mineral deposits are arranged as a system of struts. Bone marrow fill the spaces between. ! *Bone marrow. Some bones, such as the femur, also contain a central cavity filled with bone marrow. Bone marrow contains the stem cells that gives rise to all the types of blood cells. ! *. Prior to puberty, this disk of cartilage produces more cartilage which then is converted into more bone. In this way, the bone grows lengthwise.

Osteoclasts (under the influence hormones, destroy bone) , are multinucleated giant cells, found within bone marrow, or adjacent to bone.! : is the pink matrix composed of type 1 collagen fibers and ground substance ( and osteocalcin) and made by When osteoid gets mineralized (calcium) = calcified bone

Osteoblasts secrete alkaline phosphatase secrete: Tartrate resistant acid phosphatase (TRAP)

Resting chondrocytes!

Proliferating chondrocytes!

Maturing chondrocytes!

Zone of calcification! Synovial joint space!

hypertrophied cartilage zone!

Calcified cartilage zone!

ossification zone!

Bone marrow

Bone and marrow with precursor bone forming cells at different stages of development!

Osteoblasts (build bone) are found lining bony spicules! Histo-chemistry methods Examples: Alizarin Red and Alcian blue on cleared embryos to examine bone and cartilage carefully

Safranin-O may also be used Clear tissues and stain with AlizarinRed and Alcian Blue for bone and cartilage!

Histochemical stains review • Alcian Blue: mucin and for cartilage—blue • Alizarin Red: Bone—red • Elastic stain: elastic fibers—black • Giemsa on blood smears • Oil Red O: for stored lipids—red--only on FROZEN sections • PAS: basement membranes—colon control magenta mucins • PTAH for striations—black, skeletal and cardiac • Reticulin--black • Safranin-O: Cartilage--red • Sirius Red: for collagen--red • Trichrome: for collagen—colon control (blue) • Von Kossa: calcifications, black A joint (the place where two bones meet) is surrounded by a capsule that protects and supports it. The joint capsule is lined with a type of tissue called synovium, which produces that lubricates and nourishes joint tissues. In rheumatoid , the synovium becomes inflamed, causing warmth, redness, swelling, and pain. As the disease progresses, the inflamed synovium invades and damages the cartilage and bone of the joint. Surrounding muscles, ligaments, and tendons become weakened. also can cause more generalized bone loss that may lead to osteoporosis http://www.niams.nih.gov/hi/topics/ (fragile bones that are prone to fracture). Degenerative Joint disease

http://www.umm.edu/patiented/articles/ what_osteoarthritis_000035_1.htm Normal vertebral bone Osteoporotic vertebral bone This is the synovium in rheumatoid arthritis.

There is chronic inflammation with lymphocytes and plasma cells that produce the blue areas beneath the nodular proliferations.

This "pannus" is destructive and produces erosion of the articular cartilage, eventually destroying the joint. Gouty arthritis results from Chronic gout leads to deposion of deposition of sodium urate urates into a chalky mass known crystals in joints. The joint most as a "tophus". Such tophi can often affected is the first MP joint destroy the joint and adjacent bone (big toe) as seen here. as seen here radiographically in sequential radiographs of the same foot). In most, but not all, cases there is hyperuricemia. Osteosarcoma . ! The tissue is recognizable as cartilage, and there are chondrocytes in clear spaces, but there is no orderly pattern. At the bottom, this can be seen invading and destroying bone. : Skin, mammary glands, anterior pituitary, internal ear, corneal epithelium, lens Neuroectoderm: neural crest, melanocytes, neural tube, nerves, adrenal medulla, Schwann cells, retina, pineal body, posterior pituitary

ENDODERM: Epithelium of , bronchi, lungs, GI Tract, liver, pancreas, urachus, , thyroid gland, tympanic cavity, tonsils, parathyroids

MESODERM:Muscles, connective tissue, Bone, serous membranes, blood and lymph cells, spleen cardiovascular and lymphatic systems, urogenital system, including gonads, ducts and accessory glands.