143 Notul. odonatol., Vol. 1, No. 9, pp. 141-156, June I, 1982

The chromosome numbers of sixteen species from the

Arun Valley, eastern Nepal

B. Kiauta and M. Kiauta

Department of Cytogenetics and Cytotaxonomy, University of Utrecht, Padualaan 8,

Utrecht, The Netherlands

The male Abstract — list of spp. of which tillarga(Fabr.) were not previously reported

chromosome numbers are reported and brief from Nepal.

descriptive notes on the karyotypic morpho-

logy provided includes Calicnemia miniata Introduction

Sel. (2n = 25, n = 13, m) (Platycnemididae), The following is the list of species collected

= = Anax guttatus (Ramb.) (2n 15, n 8, m, April 18-30, 1979 in the Upper Arun Valley,

XO) (Aeshnidae) and intermedia Kosi Zone, eastern Nepal. Calicnemia minia-

Sel. = 13. all of Neurothem degener (n m) (). ta, Anax guttatus and is inter-

A. which are new for cytology. guttatus is media degenerare new for cytology. The first

the aberrant member the cytologically most of mentioned species and Tholymis tillarga were

and genus so far examined, micrographs are not recorded previously from the Nepalese

shown of its divisional C. the principal stages. territory; others are listed here for the sake

found miniata was breeding on permanently of distributional records only. All specimens

rocks covered and Dr wet by forest litter moss, were examined by M.A. Lieftinck,

without open water. This sp. and Tholymis Rhenen. The Feulgen slides and specimens Notul. Vol. June 144 odonatol., I. No. 9, pp. 141-156, I, 1982

are/will be deposited in the authors' collec- gradation is more or less pronounced, and the

tion. is smallest of sex element always the the set, or,

at most, equal to m at primary spermatocyte

list — Annotated metaphase. (3) It is perhaps worthwhile to

Platycnemididae — Calicnemia miniata mention that we have found C. miniata

also Sel. (3 S- in a forest between the Irkua and breedingin a similar habitat at Kodari, on

Bokua The Kholas, 550 m, Apr. 27). habitat is the Nepal-Tibet frontier (1700 m, June 4,

wet, shady forest ground, without open water. 1973), where it is accompanied by C. eximia

Larvae were collected on permanently wet Sel. and by the above mentioned undescribed rocks, covered by organic litter, mud and species. The latter two species breed also in

Teneral individuals and exuviae waterfalls, but differ in this from C. moss. were respect

also the time of visit. 2n = present at our — 25, pulverulans, which wehave always collected in

of n = 13, m-bivalent similar or larger size than rapid streams of the Kathmandu Valley

I X at m (cf. Fig. I). (Godavari) only.

Coenagrionidae Ceriagrion coro-

mandelianum (Fabr.) (2 <5, Katikyegat, right

bank of Arun, North of Tumlingtar, 425 m.

= Apr. 28). — n 14. The smallest bivalent at m

and I hardly recognizable as an m, equal in size

to X.

Chlorocyphidae — The generalkary-

otypic features of the three species listed below

are identical, viz. n = 12. m: at m I, one

bivalent distinctly large.

Rhinocypha quadrimaculata Sel. (I

bank of Arun, 445 Fig. I. Calicnemia miniala Sel., $ (Platycne- Dingla area, right m, Apr.

— = There 13 in the mididae): primary spermatocyte metaphase. 29). are no n complements

specimen examined (cf. KIAUTA. 1975, p.

42). At m X the second smallest ofthe m NOTES: (I) At the moment of the 1, set,

minute. preparation of this note. Dr M.A. Lieftinck

R. Sel. (2 Khola, has largely completed his taxonomic revision trifasciata $. Sanghua of the For this he has track. 600 m, Apr. 26). At genus. purpose re- Japhu-Bumling

I, and X than in the examined all of our previously published m m significantly larger

former in Calicnemia material from Nepal, and has preceding species; the many figures

from the second that the of hardly distinguishable informed us (Dec. 6. 1981) names

smallest bivalent. two taxa. published earlier by KIAUTA

be corrected. R. unimaculata Sel. (4 <5. Dingla area, (1975), should The correct name

bank ofArun, Chauabesi,445 of ”C. nipalica”. listed in the said publication, right opposite m,

and C. 29; 1 S Sanghua Khola. Japhu-Bumling p. 43 pp. 72-73, fig. I. is pulverulans Apr.

2n = At track, 600 m. Apr. 26). 23. m I. m Sel., while the material identified as "Calicne-

is and X usually large. In some m-l figures there mia sp." (p. 43 and pp. 72-73. fig. 3) pertain-

should are 13 or more elements, due to ing to an undescribed species. (2) It precocious

of the also segregation one or more of medium- be emphasized here, as is apparent from

-sized bivalents. the above mentioned fig. 3. that the karyotype

Euphaeidae Anisopleura comes of the new species is significantly different

Sel. (1 <5, Khola, from all other so far known Calicnemia Sanghua Japhu-Bumling

n = 13, karyotypes. At variance with other Calicne- track, 600 m, Apr. 26). m.

chi- Calopterygidae Neurohasis c. mia laxa, the metaphase-lelements in the new

nensis (L.) (2 (5. Dingla area, right bank of species, including the univalent sex chromo-

Arun, Chauabesi, 445 m, 29). some, are almost uniform is si/e. In all other opposite Apr.

the 2n = 23, n = 12, m. examined members of genus the size Notul. Vol. No. 141-156, June 1, 1982 145 odonatol., 1, 9, pp.

— — sobrina Aeshnidae Anax guttatus(Burm.) (1 Libellulidae Brachydiplax

<5, Katikyegat, right bank of Arun, North of (Ramb.) (1 5, Katikyegat, right bank ofArun,

— = — Tumlingtar, 425 m, Apr. 28). 2n= 15, n 8, North of Tumlingtar, 425 m, Apr. 28).

m, XO. This is a secondary complement, 2n = 25, n= 13, m. originating in fusion 2-by-2 of the primary Crocothemis servilia (Dru.) ( 1 $, rice fields

left bank of karyotype autosomes, leaving the m-elements between Hile-Tumlingtar, Arun,

= and the X unaffected. Consequently, the 370 m, Apr. 14). — n 13. m. pachytene and anaphase-II behaviour of the X Diplacodes nebulosa (Fabr.). (2 $, rice

is normal (cf. Figs 2-5), This is cytologically fields between Hile-Tumlingtar, left bank of

= m the most aberrant species yet encountered in Arun, 370 m, Apr. 18). n 13, (largeat m I).

the Neurothemis intermedia Sel. genus. degener (I 5.

= Tumlingtar, 400 m, Apr. 30). — n 13, m.

Orthetrum luzonicum Br. (I 5. Japhu, 680

= = m m, Apr. 25) — 2n 25, n 13, m. At I, X is

the second smallest of the set.

O. pruinosum neglectum (Ramb.) (1 5.

Japhu-Bumling track, 600 m, Apr. 26). —

n= 13, m.

Tholymis tillarga(Fabr.) (2 5, Katikyegat,

right bank of Arun, North ofTumlingtar,425

= = m, Apr. 28). — 2n 25, n 13, m (minute).

Tramea basilaris burmeisteri Kirby (2 5.

Katikyegat, right bank of Arun, north of

= Tumlingtar,425 m, Apr. 28). — n 13, m (at

m 1 similar in size to X).

Reference — KIAUTA, B„ 1975. Cytotaxo-

nomy of with special reference to Figs 2-5. Anax (Ramb.), $ (Aeshni- gutlalus the Nepalesefauna. Nepal Research Center, dae), stages of maturation divisions, principal Kathmandu. the mode showing XO ofsex determination in

the secondary karyotype: (2) spermatogonial Received January 4, 1982

metaphase;— (3) pachytene; — (4) metaphase

I; — (5) early anaphase 11, lateral view.