Publications Collection for Thermo Scientific High Content Products
2013 Edition / Version 2 What is High Content Screening (HCS)? High content screening (HCS), also known as high content analysis, image cytometry, quantitative cell analysis or automated cell analysis, is an automated method that is used to identify substances that alter the phenotype of a cell in a desired manner. This technology is primarily used in biological research and drug discovery and combines fluorescent microscopy, automated cell calculations, and phenotyping using image processing algorithms and informatics tools for the user to make decisions about a treatment.
Thermo Scientific High Content Products The portfolio of High Content Analysis products includes assay development and screening tools like the Thermo Scientific™ ArrayScan™ XTI High Content Analysis (HCA) Reader and the Thermo Scientific™ CellInsight™ NXT High Content Screening (HCS) Platform. Multiple tools are available for assay development on the ArrayScan XTI HCA Reader like the Thermo Scientific™ X1 large field-of-view, high resolution camera; Live Cell Chamber; and the new Confocal Module, while our software products like Thermo Scientific™ HCS Studio™ Software enable users to develop and make decisions about our assays. With the Thermo Scientific™ HCS101 Class and the diverse portfolio of reagents and consumables, we enable scientists to increase their efficiency with their platform while generating more knowledge about the cell.
More knowledge about cellular information • More measurements and data about cells More knowledge evolved into products and their response • More out-of-the-box reagents validated Cellular • More information than other cell-based assays for high content Information • More flexibility in instrumentation to address new assay needs More knowledge about cells in their context • More information in the context of a Validated Contextual living cell SOlutions Relevance • More tools to characterize complex more biologies Knowledge
More knowledge in how to execute HCA Scientific validity though literature • More technical resources focused on • More peer-reviewed publications in the high content Experienced Scientific most relevant and respected journals Execution Validity • More experience in using, developing, • Automated solutions to increase throughput and executing on high content assays
For more information on Thermo Scientific™ High Content Products, and a full list of applications by application area, go to www.thermoscientific.com/highcontent Applications in High Content Thermo Scientific High Content Tools have been used in hundreds of different applications in all types of laboratories across the world. The unique technology that High Content provides, along with the value that Thermo Scientific Products bring to scientists, enable discoveries across a broad range of applications. This collection of applications demonstrates the sheer number and breadth of peer-reviewed work on Thermo Scientific High Content Products since High Content was introduced in the late 1990s. Number of Publications per Year 180 The Growth of Applications for High Content Published Application Areas Using 160 Focused originally around screening applications in drug Thermo Scientific High Content Products 140 discovery, High Content Technology addressed more 50+ 120 simple assays in signaling pathways, cell health, and 100 proliferation applications. As the technology grew in 80 adoption and capabilities through the 2000s, the number 60 of assays addressable by High ContentNumber grew asof well.Publications per Year 40 180In 2013, there are 50+ application areas represented 20 20 in published literature, demonstrating the breadth and Published Application Areas Using 0 160 Thermo Scientific High Content Products 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 broad 2008 functionality2009 2010 of2011 the 2012 Thermo Scientific High Content 140 Platforms. 3 Cumulative Publications Per Year using Thermo Scientific120 High Content Platforms 50+ 1999 2005 2013 900 100 Core application areas (Cytotoxicity, Signaling, Angiogenesis, etc.) addressed 800 P80eer-Reviewed Publications Support Technology over the years with Thermo Scientific High Content Products. 60 700 Thermo Scientific High Content Products: •40 Average 115+ peer-reviewed publications per year (2010–2012) 600 20 •20 Average more than nine publications per month since 2010 500 • Have 30% of publications in some of the most prestigious science journals (e.g., Cell, PNAS, Nature) 0 400 • Have1997 >50% 1998 of papers 1999 in 2000 some 2001of the 2002most prestigious 2003 2004 cell 2005 biology 2006 journals 2007 2008 2009 2010 2011 2012
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200 Cumulative Publications Per Year using Thermo Scientific High Content Platforms 1999 2005 2013 900 100
0 800 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 700
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With a rate of more than 115 new peer-reviewed publications per year (2010–2012), we offer the most published platform for High Content Analysis. publications Collection
1. Abdelwahab, S.I. et al. Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evid. Based Complement. Alternat. Med. 2012 (2012).
2. Aberg, M., Johnell, M., Wickstrom, M. & Siegbahn, A. Tissue Factor/ FVIIa Prevents The Extrinsic Pathway of Apoptosis by Regulation Of The Tumor Suppressor Death-Associated Protein Kinase 1 (DAPK1). Thromb. Res. 127, 141-148 (2011).
3. Aberg, M., Johnell, M., Wickstrom, M., Widunder, A. & Siegbahn, A. Simvastatin Reduces the Production Of Prothrombotic Prostasomes in Human Prostate Cancer Cells. Thromb. Haemost. 100, 655-662 (2008).
4. Aberg, M., Wickstrom, M. & Siegbahn, A. Simvastatin Induces Apoptosis In Human Breast Cancer Cells in a NFκB-Dependent Manner And Abolishes The Anti-Apoptotic Signaling of TF/FVIIa and TF/FVIIa/FXa. Thromb. Res. 122, 191-202 (2008).
5. Abraham, V.C., Samson, B., Lapets, O. & Haskins, J.R. Automated Classification of Individual Cellular Responses Across Multiple Targets. Preclinica 2, 349-355 (2004).
6. Abraham, V.C., Taylor, D.L. & Haskins, J.R. High Content Screening Applied to Large-Scale Cell Biology. Trends Biotechnol. 22, 15-22 (2004).
7. Abraham, V.C., Towne, D.L., Waring, J.F., Warrior, U. & Burns, D.J. Application of a High-Content Multiparameter Cytotoxicity Assay to Prioritize Compounds Based on Toxicity Potential in Humans. J. Biomol. Screen. 13, 527-537 (2008).
8. Achiwa, H. & Lazo, J.S. PRL-1 Tyrosine Phosphatase Regulates c-Src Levels, Adherence, and Invasion in Human Lung Cancer Cells. Cancer Res. 67, 643-650 (2007).
9. Achoui, M. et al. In Vitro and In Vivo Anti-Inflammatory Activity of 17-O-Acetylacuminolide through the Inhibition of Cytokines, NF-κB Translocation and IKKβ Activity. PLoS One 5 (2010).
10. Agler, M. et al. A High-Content Glucocorticoid Receptor Translocation Assay for Compound Mechanism-of- Action Evaluation. J. Biomol. Screen. 12, 1029-1041 (2007).
11. Albert, B.J. et al. Meayamycin Inhibits pre-mRNA Splicing and Exhibits Picomolar Activity Against Multidrug Resistant Cells. Mol. Cancer Ther. 8, 2308-2318 (2009).
12. Aldridge, B.B., Gaudet, S., Lauffenburger, D.A. & Sorger, P.K. Lyapunov Exponents and Phase Diagrams Reveal Multi-Factorial Control Over TRAIL-Induced Apoptosis. Mol. Syst. Biol. 7, 553 (2011).
13. Alejandro, E.U. & Johnson, J.D. Inhibition of Raf-1 Alters Multiple Downstream Pathways to Induce Pancreatic -Cell Apoptosis. J. Biol. Chem. 283, 2407-2417 (2008).
14. Alejandro, E.U. et al. Acute Insulin Signaling in Pancreatic Beta-Cells Is Mediated by Multiple Raf-1 Dependent Pathways. Endocrinology 151, 502-512 (2010).
15. Alejandro, E.U. et al. Pancreatic β-cell Raf-1 is Required for Glucose Tolerance, Insulin Secretion, and Insulin 2 Transcription. FASEB J. 25, 3884-3895 (2011).
16. Alexander, L. et al. Inhibition of Envelope-Mediated CD4+-T-Cell Depletion by Human Immunodeficiency Virus Attachment Inhibitors. Antimicrob. Agents Chemother. 53, 4726-4732 (2009). 17. Alvarez-Fernandez, M., Halim, V.A., Aprelia, M., Mohammed, S. & Medema, R.H. Protein Phosphatase 2A (B55α) Prevents Premature Activation of Forkhead Transcription Factor FoxM1 by Antagonizing Cyclin A/Cyclin- dependent Kinase-mediated Phosphorylation. J. Biol. Chem. 286, 33029-33036 (2011).
18. Anderson, L.J., Lin, K., Compton, T. & Wiedmann, B. Inhibition of Cyclophilins Alters Lipid Trafficking and Blocks Hepatitis C Virus Secretion. Virol J. 8, 329 (2011).
19. Anderton, M.J. et al. Induction of Heart Valve Lesions by Small-Molecule ALK5 Inhibitors. Toxicol. Pathol. 39, 916-924 (2011).
20. Ang, F., Wong, A.P.Y., Ng, M.M.L. & Chu, J.J.H. Small Interference RNA Profiling Reveals The Essential Role Of Human Membrane Trafficking Genes In Mediating The Infectious Entry Of Dengue Virus. Virol J. 7, 24 (2010).
21. Annes, J.P. et al. Adenosine Kinase Inhibition Selectively Promotes Rodent And Porcine Islet β-Cell Replication. Proc. Natl. Acad. Sci. U. S. A. 109, 3915-3920 (2012).
22. Arsic, N. et al. A novel function for Cyclin A2: Control of cell invasion via RhoA signaling. J. Cell Biol. 196, 147- 162 (2012).
23. Arya, A. et al. Chloroform Fraction of Centratherum anthelminticum (L.) Seed Inhibits Tumor Necrosis Factor Alpha and Exhibits Pleotropic Bioactivities: Inhibitory Role in Human Tumor Cells. Evid. Based Complement. Alternat. Med. 2012 (2012).
24. Asada, M. et al. DNA Binding - Dependent Glucocorticoid Receptor Activity Promotes Adipogenesis Via Krüppel-Like Factor 15 Gene Expression. Lab. Invest. 91, 203-215 (2011).
25. Astle, M.V. et al. AKT Induces Senescence In Human Cells Via mTORC1 and p53 in the Absence of DNA Damage: Implications For Targeting mTOR During Malignancy. Oncogene 31, 1949-1962 (2012).
26. Atienzar, F.A. et al. The Use of Real-Time Cell Analyzer Technology in Drug Discovery: Defining Optimal Cell Culture Conditions and Assay Reproducibility with Different Adherent Cellular Models. J. Biomol. Screen. 16, 575- 587 (2011).
27. Auld, K.L. et al. Estrogen-Related Receptor α Regulates Osteoblast Differentiation Via Wnt/β-Catenin Signaling. J. Mol. Endocrinol. 48, 177-191 (2012).
28. Ayoub, N. et al. The Carboxyl Terminus of Brca2 Links the Disassembly of Rad51 Complexes to Mitotic Entry. Curr. Biol. 19, 1075-1085 (2009).
29. Azzariti, A. et al. Aurora B Kinase Inhibitor AZD1152: Determinants of Action and Ability to Enhance Chemotherapeutics Effectiveness in Pancreatic and Colon Cancer. Br. J. Cancer 104, 769-780 (2011).
30. Baba, T. et al. A Rat Model for Choroidal Neovascularization Using Subretinal Lipid Hydroperoxide Injection. Am. J. Pathol. 176, 3085-3097 (2010).
31. Badura, L. et al. An Inhibitor of Casein Kinase Iε Induces Phase Delays in Circadian Rhythms under Free- Running and Entrained Conditions. J. Pharmacol. Exp. Ther. 322, 730-738 (2007).
32. Baffert, F. et al. Potent and Selective Inhibition of Polycythemia by the Quinoxaline JAK2 Inhibitor NVP- BSK805. Mol. Cancer Ther. 9, 1945-1955 (2010).
33. Bagchi, S. et al. The P2Y2 Nucleotide Receptor Interacts with αv Integrins to Activate Go and Induce Cell Migration. J. Biol. Chem. 280, 39050-39057 (2005). 34. Bajenaru, M.L. et al. β1 Integrin-Focal Adhesion Kinase (FAK) Signaling Modulates Retinal Ganglion Cells Survival. Invest. Ophthalmol. Vis. Sci. 53, 3482- (2012).
35. Banwell, E.F. et al. Rational Design And Application Of Responsive α-Helical Peptide Hydrogels. Nat Mater 8, 596-600 (2009).
36. Bao, L. et al. Pre-Clinical Development Of A Bi-Functional Cancer Cell Homing, PKCε Inhibitory Peptide For The Treatment Of Head And Neck Cancer. Cancer Res. 69, 5829-5834 (2009).
37. Bao, R. et al. Targeting Heat Shock Protein 90 with CUDC-305 Overcomes Erlotinib Resistance in Non-Small Cell Lung Cancer. Mol. Cancer Ther. 8, 3296-3306 (2009).
38. Bao, Y. et al. A Cell-Based Assay to Screen Stimulators of the Hippo Pathway Reveals the Inhibitory Effect of Dobutamine on the YAP-Dependent Gene Transcription. J. Biochem. 150, 199-208 (2011).
39. Bardelle, C. & Boros, J. Development of a High-Content High-Throughput Screening Assay for the Discovery of ATM Signaling Inhibitors. J. Biomol. Screen. 17, 912-920 (2012).
40. Barnard, R., Barnard, A., Salmon, G., Liu, W. & Sreckovic, S. Histamine-Induced Actin Polymerization in Human Eosinophils: An Imaging Approach For Histamine H4 Receptor. Cytometry A 73, 299-304 (2008).
41. Barrows, N.J., Sommer, C.L., Garcia-Blanco, M.A. & Pearson, J.L. Factors Affecting Reproducibility between Genome-Scale siRNA-Based Screens. J. Biomol. Screen. 15, 735-747 (2010).
42. Bartlett, N.W. et al. A New Member of The Interleukin 10-Related Cytokine Family Encoded By A Poxvirus. J. Gen. Virol. 85, 1401-1412 (2004).
43. Basu, P., Ghosh, R.N., Grove, L.E., Klei, L. & Barchowsky, A. Angiogenic Potential of 3-Nitro-4-Hydroxy Benzene Arsonic Acid (Roxarsone). Environ. Health Perspect. 116, 520-523 (2008).
44. Bauer, P.O. et al. Inhibition of Rho Kinases Enhances the Degradation of Mutant Huntingtin. J. Biol. Chem. 284, 13153-13164 (2009).
45. Baum, P. et al. Phenocopy – A Strategy to Qualify Chemical Compounds During Hit-to-Lead and/or Lead Optimization. PLoS One 5 (2010).
46. Bedard, K.M. et al. Isoflavone Agonists of IRF-3 Dependent Signaling Have Antiviral Activity against RNA Viruses. J. Virol. 86, 7334-7344 (2012).
47. Bednarek, R. et al. Ku80 as a Novel Receptor for Thymosin 4 That Mediates Its Intracellular Activity Different from G-actin Sequestering. J. Biol. Chem. 283, 1534-1544 (2008).
48. Behfar, A. et al. Cardiopoietic Programming Of Embryonic Stem Cells For Tumor-Free Heart Repair. J. Exp. Med. 204, 405-420 (2007).
49. Beith, J.L., Alejandro, E.U. & Johnson, J.D. Insulin Stimulates Primary β-Cell Proliferation via Raf-1 Kinase. Endocrinology 149, 2251-2260 (2008).
50. Benjannet, S. et al. Effects of the Prosegment and pH on the Activity of PCSK9: Evidence For Additional Processing Events. J. Biol. Chem. 285, 40965-40978 (2010).
51. Berlinicke, C.A. et al. High-Content Screening Data Management For Drug Discovery in a Small- to Medium-Size Laboratory: Results of a Collaborative Pilot Study Focused on User Expectations as Indicators of Effectiveness. J. Lab. Autom. 17, 255-265 (2012). 52. Bertelsen, M. Multiplex Analysis of Inflammatory Signaling Pathways Using A High-Content Imaging System. Methods Enzymol. 414, 348-363 (2006).
53. Bertelsen, M. & Sanfridson, A. Inflammatory Pathway Analysis Using A High Content Screening Platform. Assay Drug Dev. Technol. 3, 261-271 (2005).
54. Bhawe, K.M., Blake, R.A., Clary, D.O. & Flanagan, P.M. An automated Image Capture and Quantitation Approach to Identify Proteins Affecting Tumor Cell Proliferation. J. Biomol. Screen. 9, 216-222 (2004).
55. Bier, C. et al. Allosteric Inhibition of Taspase1’s Pathobiological Activity By Enforced Dimerization In Vivo. FASEB J. 26, 3421-3429 (2012).
56. Bierut, L.J. et al. Novel Genes Identified In A High-Density Genome Wide Association Study for Nicotine Dependence. Hum. Mol. Genet. 16, 24-35 (2007).
57. Bilodeau, S., Kagey, M.H., Frampton, G.M., Rahl, P.B. & Young, R.A. SetDB1 Contributes to Repression Of Genes Encoding Developmental Regulators And Maintenance of ES Cell State. Genes Dev. 23, 2484-2489 (2009).
58. Bjorklund, M. et al. Identification of Pathways Regulating Cell Size and Cell-Cycle Progression by RNAi. Nature 439, 1009-1013 (2006).
59. Blackmore, M.G. et al. High Content Screening of Cortical Neurons Identifies Novel Regulators of Axon Growth. Mol. Cell. Neurosci. 44, 43-54 (2010).
60. Blackmore, M.G. et al. Krüppel-Like Factor 7 Engineered for Transcriptional Activation Promotes Axon Regeneration in the Adult Corticospinal Tract. Proc. Natl. Acad. Sci. U. S. A. 109, 7517-7522 (2012).
61. Blin, G. et al. A Purified Population of Multipotent Cardiovascular Progenitors Derived From Primate Pluripotent Stem Cells Engrafts in Postmyocardial Infarcted Nonhuman Primates. J. Clin. Invest. 120, 1125-1139 (2010).
62. Boeckeler, K., Rosse, C., Howell, M. & Parker, P.J. Manipulating Signal Delivery - Plasma-Membrane ERK Activation in aPKC-Dependent Migration. J. Cell Sci. 123, 2725-2732 (2010).
63. Bonner, D.K. et al. Intracellular Trafficking of Polyamidoamine – Polyethylene Glycol Block Copolymers in DNA Delivery. Bioconjug. Chem. 22, 1519-1525 (2011).
64. Borchert, K.M. et al. High-Content Screening Assay for Activators of the Wnt/Fzd Pathway In Primary Human Cells. Assay Drug Dev. Technol. 3, 133-141 (2005).
65. Borikova, A.L. et al. Rho Kinase Inhibition Rescues the Endothelial Cell Cerebral Cavernous Malformation Phenotype. J. Biol. Chem. 285, 11760-11764 (2010).
66. Borner, G.H.H. et al. Multivariate Proteomic Profiling Identifies Novel Accessory Proteins of Coated Vesicles. J. Cell Biol. 197, 141-160 (2012).
67. Bouck, D.C., Shu, P., Cui, J., Shelat, A. & Chen, T. A High Content Screen Identifies Inhibitors of Nuclear Export of Forkhead Transcription Factors. J. Biomol. Screen. 16, 394-404 (2011).
68. Brandstaetter, H., Kendrick-Jones, J. & Buss, F. Myo1c Regulates Lipid Raft Recycling to Control Cell Spreading, Migration And Salmonella Invasion. J. Cell Sci. 125, 1991-2003 (2012).
69. Brave, S.R. et al. Assessing the Activity of Cediranib, a VEGFR-2/3 Tyrosine Kinase Inhibitor, against VEGFR-1 and Members of the Structurally Related PDGFR Family. Mol. Cancer Ther. 10, 861-873 (2011). 70. Breier, J.M., Radio, N.M., Mundy, W.R. & Shafer, T.J. Development of a High-Throughput Screening Assay for Chemical Effects on Proliferation and Viability of Immortalized Human Neural Progenitor Cells. Toxicol. Sci. 105, 119-133 (2008).
71. Brew, C.T. et al. Indole-3-Carbinol Inhibits MDA-MB-231 Breast Cancer Cell Motility and Induces Stress Fibers and Focal Adhesion Formation by Activation of Rho Kinase Activity. Int. J. Cancer 124, 2294-2302 (2009).
72. Brickelmaier, M. et al. Identification and Characterization of Mefloquine Efficacy against JC Virus In Vitro. Antimicrob. Agents Chemother. 53, 1840-1849 (2009).
73. Brisson, M. et al. Discovery and Characterization of Novel Small Molecule Inhibitors of Human Cdc25B Dual Specificity Phosphatase. Mol. Pharmacol. 66, 824-833 (2004).
74. Brummond, K.M., Mao, S., Shinde, S.N., Johnston, P.J. & Day, B.W. Design and Synthesis of a Library of Tetracyclic Hydroazulenoisoindoles. J. Comb. Chem. 11, 486-494 (2009).
75. Bruner, J. et al. Work-Cell Approach to Automating High Content Screening. JALA 8, 66-67 (2003).
76. Buchser, W.J., Laskow, T.C., Pavlik, P.J., Lin, H.-M. & Lotze, M.T. Cell-Mediated Autophagy Promotes Cancer Cell Survival. Cancer Res. 72, 2970-2979 (2012).
77. Buchser, W.J., Pardinas, J.R., Shi, Y., Bixby, J.L. & Lemmon, V.P. 96-Well Electroporation Method For Transfection Of Mammalian Central Neurons. BioTechniques 41, 619-624 (2006).
78. Buchser, W.J., Slepak, T.I., Gutierrez-Arenas, O., Bixby, J.L. & Lemmon, V.P. Kinase/Phosphatase Overexpression Reveals Pathways Regulating Hippocampal Neuron Morphology. Mol. Syst. Biol. 6, 391 (2010).
79. Buckbinder, L. et al. Proline-Rich Tyrosine Kinase 2 Regulates Osteoprogenitor Cells and Bone Formation, and Offers an Anabolic Treatment Approach for Osteoporosis. Proc. Natl. Acad. Sci. U. S. A. 104, 10619-10624 (2007).
80. Buglio, D. et al. Essential Role of TAK1 in Regulating Mantle Cell Lymphoma Survival. Blood 120, 347-355 (2012).
81. Cappell, K.M. et al. Multiple Cancer Testis Antigens Function To Support Tumor Cell Mitotic Fidelity. Mol. Cell. Biol. 32, 4131-4140 (2012).
82. Carter, C.A. Multiplexed High Content Screening Reveals That Cigarette Smoke Condensate-Altered Cell Signaling Pathways Are Accentuated Through FAK Inhibition in Human Bronchial Cells. Science 336, 1186-a- (2012).
83. Cathcart, M.C. et al. Examination of Thromboxane Synthase as a Prognostic Factor and Therapeutic Target In Non-Small Cell Lung Cancer. Mol. Cancer 10, 25 (2011).
84. Chan, E.Y.W., Longatti, A., McKnight, N.C. & Tooze, S.A. Kinase-Inactivated ULK Proteins Inhibit Autophagy via Their Conserved C-Terminal Domains Using an Atg13-Independent Mechanism. Mol. Cell. Biol. 29, 157-171 (2009).
85. Chantong, B., Kratschmar, D.V., Nashev, L.G., Balazs, Z. & Odermatt, A. Mineralocorticoid and Glucocorticoid Receptors Differentially Regulate NF-κB activity and Pro-Inflammatory Cytokine Production In Murine BV-2 Microglial Cells. J. Neuroinflammation 9, 260 (2012).
86. Chen, H.-W. et al. Curcumin Inhibits Lung Cancer Cell Invasion and Metastasis through the Tumor Suppressor HLJ1. Cancer Res. 68, 7428-7438 (2008). 87. Chen, J. et al. An RNAi Screen Identifies Additional Members Of The Drosophila Integrator Complex And A Requirement For Cyclin C/Cdk8 in snRNA 3’-End Formation. RNA 18, 2148-2156 (2012).
88. Chen, J. et al. The Bcl-2/Bcl-XL/Bcl-w Inhibitor, Navitoclax, Enhances the Activity of Chemotherapeutic Agents In Vitro and In Vivo. Mol. Cancer Ther. 10, 2340-2349 (2011).
89. Chen, Y. et al. Developing and Applying A Gene Functional Association Network For Anti-Angiogenic Kinase Inhibitor Activity Assessment In An Angiogenesis Co-Culture Model. BMC Genomics 9, 264 (2008).
90. Chen, Z. et al. 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) Induces Phosphorylation of Eukaryotic Elongation Factor-2 (eEF2): A Cautionary Note on the Anticancer Mechanism of an eEF2 Kinase Inhibitor. J. Biol. Chem. 286, 43951-43958 (2011).
91. Cheng, F. et al. KSHV Reactivation from Latency Requires Pim-1 and Pim-3 Kinases to Inactivate the Latency- Associated Nuclear Antigen LANA. PLoS Pathog. 5 (2009).
92. Cheng, S.M. et al. HIV-1 Transactivator Protein Induction of Suppressor of Cytokine Signaling-2 Contributes to Dysregulation of IFNγ Signaling. Blood 113, 5192-5201 (2009).
93. Cheung, P. & Dennis, J.W. Mgat5 and Pten Interact to Regulate Cell Growth and Polarity. Glycobiology 17, 767-773 (2007).
94. Cheung, P. et al. Metabolic Homeostasis And Tissue Renewal Are Dependent on β1,6GlcNAc-Branched N-Glycans. Glycobiology 17, 828-837 (2007).
95. Chow, A. et al. Polarized Secretion of Interleukin (IL)-6 and IL-8 by Human Airway Epithelia 16HBE14o- Cells in Response to Cationic Polypeptide Challenge. PLoS One 5 (2010).
96. Chresta, C.M. et al. AZD8055 Is a Potent, Selective, and Orally Bioavailable ATP-Competitive Mammalian Target of Rapamycin Kinase Inhibitor with In vitro and In vivo Antitumor Activity. Cancer Res. 70, 288-298 (2010).
97. Chuong, C., Katz, J., Pauley, K.M., Bulosan, M. & Cha, S. RAGE Expression and NF-κB Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line. J. Cell. Physiol. 221, 430-434 (2009).
98. Ciossek, T., Julius, H., Wieland, H., Maier, T. & Beckers, T. A Homogeneous Cellular Histone Deacetylase Assay Suitable For Compound Profiling And Robotic Screening. Anal. Biochem. 372, 72-81 (2008).
99. Cipres, A. et al. Sceptrin, a Marine Natural Compound, Inhibits Cell Motility in a Variety of Cancer Cell Lines. ACS Chem. Biol. 5, 195-202 (2010).
100. Claus, C., Tzeng, W.P., Liebert, U.G. & Frey, T.K. Analysis of the Selective Advantage Conferred by a C-E1 Fusion Protein Synthesized by Rubella Virus DI RNAs. Virology 369, 19-34 (2007).
101. Clement, C.M., Dandepally, S.R., Williams, A.L. & Ibeanu, G.C. A Synthetic Analog Of Verbenachalcone Potentiates NGF-Induced Neurite Outgrowth And Enhances Cell Survival In Neuronal Cell Models. Neurosci. Lett. 459, 157-161 (2009).
102. Cole, M.F., Johnstone, S.E., Newman, J.J., Kagey, M.H. & Young, R.A. Tcf3 Is An Integral Component Of The Core Regulatory Circuitry Of Embryonic Stem Cells. Genes Dev. 22, 746-755 (2008).
103. Collins, M.A. Generating ‘Omic Knowledge’: The Role of Informatics in High Content Screening. Comb. Chem. High Throughput Screening 12, 917-925 (2009).
104. Colombo, R. et al. Targeting the Mitotic Checkpoint for Cancer Therapy with NMS-P715, an Inhibitor of MPS1 Kinase. Cancer Res. 70, 10255-10264 (2010). 105. Conway, B.R. et al. Quantitative Analysis Of Agonist-Dependent Parathyroid Hormone Receptor Trafficking In Whole Cells Using A Functional Green Fluorescent Protein Conjugate. J. Cell. Physiol. 189, 341-355 (2001).
106. Conway, B.R. et al. Quantification of G-Protein Coupled Receptor Internalization Using G-Protein Coupled Receptor-Green Fluorescent Protein Conjugates with the ArrayScanTM High-Content Screening System. J. Biomol. Screen. 4, 75-86 (1999).
107. Coppé, J.P. et al. A Human-Like Senescence-Associated Secretory Phenotype Is Conserved in Mouse Cells Dependent on Physiological Oxygen. PLoS One 5 (2010).
108. Courilleau, C. et al. The Chromatin Remodeler p400 ATPase Facilitates Rad51-Mediated Repair of DNA Double-Strand Breaks. J. Cell Biol. 199, 1067-1081 (2012).
109. Cucchi, U. et al. Phosphorylation of TCTP as a Marker for Polo-like Kinase-1 Activity In Vivo. Anticancer Res. 30, 4973-4985 (2010).
110. Culbert, A.A. et al. MAPK-activated Protein Kinase 2 Deficiency in Microglia Inhibits Pro-inflammatory Mediator Release and Resultant Neurotoxicity: Relevance To Neuroinflammation In A Transgenic Mouse Model Of Alzheimer Disease. J. Biol. Chem. 281, 23658-23667 (2006).
111. Cummings, J. et al. Preclinical Evaluation of M30 and M65 ELISAs as Biomarkers of Drug Induced Tumor Cell Death And Antitumor Activity. Mol. Cancer Ther. 7, 455-463 (2008).
112. Cuvelier, G.D. et al. Anti-CD13 Antibodies In Children With Extensive Chronic Graft-Versus-Host Disease And Their Relation To Soluble CD13 After Allogeneic Blood And Marrow Transplantation From a Children’s Oncology Groups Study, ASCT0031. Bone Marrow Transplant. 45, 1653-1657 (2010).
113. Dahl, J.P. et al. Characterization of the WAVE1 Knock-Out Mouse: Implications for CNS Development. J. Neurosci. 23, 3343-3352 (2003).
114. Davey, R.E., Onishi, K., Mahdavi, A. & Zandstra, P.W. LIF-mediated Control Of Embryonic Stem Cell Self- Renewal Emerges Due To An Autoregulatory Loop. FASEB J. 21, 2020-2032 (2007).
115. Davies, A.H. et al. YB-1 Evokes Susceptibility To Cancer Through Cytokinesis Failure, Mitotic Dysfunction, and HER2 Amplification. Oncogene 30, 3649-3660 (2011).
116. Davies, B.R. et al. Preclinical Pharmacology of AZD5363, an Inhibitor of AKT: Pharmacodynamics, Antitumor Activity, and Correlation of Monotherapy Activity with Genetic Background. Mol. Cancer Ther. 11, 873-887 (2012).
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