The Journal of Experimental Medicine pdf versions have beencorrected. Please notethat Y. EugeneChin’s nameappearedintheauthorlineasEugene incorrectly Y. Chin. The htmland Vol. 205,No.1,January21, 2008. Jay X.Tang, JonathanS.Reichner, andMinsooKim Nicole A.Morin,Patrick W. Oakes,Young-Min Hyun,DooyoungLee,Y. EugeneChin,MichaelR.King,TimothyA.Springer, MotomuS Nonmuscle myosinheavychainIIAmediatesintegrinLFA-1de-adhesionduringTlymphocytemigration Medicine ofExperimental The Journal Pages 195–205. CORRECTION
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on May 5, 2008 2008 5, May on www.jem.org from Downloaded The Journal of Experimental Medicine o.25 o , January 21, 2008 Vol. 205,No. 1, $30.00 JEM ©TheRockefeller University Press fl TIRF, totalinternalrefl siRNA, smallinterferingRNA; ROCK, Rho-associatedkinase; muscle myosinheavychainIIA; tory lightchain;MyH9,non- molecule; MLC,myosinregula- ICAM, intercellularadhesion ential interferencecontrast; Abbreviations used:DIC,diff [email protected] Kim: Minsoo CORRESPONDENCE uorescence. ection er- 195-205
Y. Eugene Motomu and ing infl cruitment todamagedorinfected tissuesdur- role inregulatingleukocyte adhesion andre- pressed onmostleukocytesand playsamajor actions. TheintegrinLFA-1 (or cell membrane receptorsthatmediatecell Integrins areafamilyof as tractionsitesoverwhichleukocytesmigrate. extracellular matrixorothercellsandfunction in infl and isacrucialstepinthedevelopmentof leukocytes iscriticalforimmunesurveillance the immunesystem.Extravasationofblood migration playsacentralroleinthefunctionof competent cellsthroughoutthebody,leukocyte Bycontributingtothepositioningofimmuno- antagonist thatinhibitsonlyaffi molecule antagonistthatinhibitsbothLFA-1affi affecting LFA-1activationbychemokineCXCL-12.Thisdefectwasreversedasmall defective taildetachment,anddecreasedlymphocytemigrationonICAM-1,without inhibition oftheassociationMyH9withLFA-1resultsinextremeuropodelongation, chain IIA(MyH9)isrecruitedtoLFA-1attheuropodofmigratingTlymphocytes, and during Tlympho that regulateseffi about integrinlymphocytefunction dynamic lymphocytemigration.Althoughagreatdealofinformationhasbeenlearned Precise spatialandtemporalregulationofcelladhesionde-adhesioniscriticalfor 4 3 2 1 NicoleA. during T lymphocytemigration LFA-1 de-adhesion mediates integrin Nonmuscle myosin heavy chainIIA link betweenLFA-1andthecytoskeletonthatiscriticalforde-adhesion. tion, uropodaladhesiondependsonLFA-1avidity, whereMyH9servesasakeymechanical cytes, whereasactiveLFA-1islocalizedtotheiranterior. Thus, duringTlymphocytemigra- revealed thatinactiveLFA-1isselectivelylocalizedtotheposteriorofpolarizedTlympho- microscopy ofthecontactzonebetweenmigratingTlymphocytesandICAM-1substrate
University ofRochester, Rochester, NY14642 and Immunology, DavidH.SmithCenter for Vaccine BiologyandImmunology, AabInstituteofBiomedicalSciences, Department ofChemicalEngineering, The CBRInstituteforBiomedicalResearch,HarvardMedicalSchool,Boston,MA02115 Department ofPhysics, BrownUniversity, Providence,RI02912 Department ofSurgery, RhodeIslandHospitalandBrownMedicalSchool,Providence,RI02903 www.jem.org/cgi/doi/ tegrins mediateleu ammatory responses.Duringmigration, – extracellular matrix,andcell Minsoo ammation.
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