For Research Use Only CD11c/ Alpha X

Polyclonal antibody www.ptgcn.com Catalog Number:17342-1-AP 24 Publications

Catalog Number: GenBank Accession Number: Recommended Dilutions: Basic Information 17342-1-AP BC038237 WB 1:500-1:1000 Size: GeneID (NCBI): IHC 1:50-1:500 300 μg/ml 3687 Source: Full Name: Rabbit integrin, alpha X (complement Isotype: component 3 receptor 4 subunit) IgG Calculated MW: Purification Method: 1169 aa, 129 kDa Antigen affinity purification Observed MW: Immunogen Catalog Number: 150 kDa AG11380

Applications Tested Applications: Positive Controls: FC, IHC, WB,ELISA WB : blood tissue; Cited Applications: IHC : human tonsillitis tissue; mouse spleen tissue, FC, IF, IHC, WB human spleen tissue Species Specificity: human, mouse Cited Species: human, mouse, pig Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Integrins are receptors that are heterodimers composed of non-covalently associated α and β Background Information subunits. CD11c/ is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including /, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in adhesion and chemotaxis.

Notable Publications Author Pubmed ID Journal Application Ryutaro Hara 33178566 Asia Pac Allergy IHC,IF Chia-Chien Hsieh 29042292 Food Chem Toxicol IHC Xiong Zhang 29028547 Int Immunopharmacol IHC

Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 4006900926 E: [email protected] W: ptgcn.com Group brand and is not available to purchase from any other manufacturer. Selected Validation Data

human blood were subjected to SDS PAGE followed Immunohistochemical analysis of paraffin- Immunohistochemical analysis of paraffin- by western blot with 17342-1-AP (ITGAX antibody) embedded human tonsillitis tissue slide using embedded human tonsillitis tissue slide using at dilution of 1:500 incubated at room temperature 17342-1-AP (CD11c/Integrin alpha X Antibody) at 17342-1-AP (CD11c/Integrin alpha X Antibody) at for 1.5 hours. dilution of 1:200 (under 10x lens). Heat mediated dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HL-60 cells were stained with 0.2ug CD11c/Integrin alpha X antibody (17342-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488- conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.