RFC2 Antibody Cat. No.: 61-510

RFC2 Antibody

Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term), 1:200 dilution,counter stained with Hematoxylin. Positive cells identified with Flow cytometry analysis of K562 cells (bottom histogram) arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery. compared to a negative control cell (top histogram).FITC- (Provided by Hirotaka Ata,University of South Alabama, conjugated goat-anti-rabbit secondary antibodies were Dept of Biochem and Mol Biol) used for the analysis.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine

This RFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 17-46 amino acids from the N-terminal region of human RFC2.

TESTED APPLICATIONS: Flow, IHC-P, WB

October 6, 2021 1 https://www.prosci-inc.com/rfc2-antibody-61-510.html For WB starting dilution is: 1:1000

APPLICATIONS: For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

PREDICTED MOLECULAR 39 kDa WEIGHT:

Properties

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by PURIFICATION: dialysis

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: RFC2

Replication factor C subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 ALTERNATE NAMES: subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40, RFC2

ACCESSION NO.: P35250

PROTEIN GI NO.: 2507300

GENE ID: 5982

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of BACKGROUND: five distinct subunits of 145, 40, 38, 37, and 36.5 kD. RFC2 is the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of RFC2 gene has been associated with Williams syndrome.

October 6, 2021 2 https://www.prosci-inc.com/rfc2-antibody-61-510.html 1) Tomida,J., J. Biol. Chem. 283 (14), 9071-9079 (2008) Gupte,R.S., Cell Cycle(2), 323-329 REFERENCES: (2005)

ANTIBODIES FOR RESEARCH USE ONLY.

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October 6, 2021 3 https://www.prosci-inc.com/rfc2-antibody-61-510.html