Anti-Human CD64 (Fc Gamma Receptor 1) SAFIRE Purified Catalog Number :06711-25 RUO: for Research Use Only

Technical Data Sheet

Anti-Human CD64 (Fc gamma Receptor 1) SAFIRE Purified Catalog Number :06711-25 RUO: For Research Use Only. Not for use in diagnostic procedures.

Product Information

Clone: 10.1 Format/Conjugate: SAFIRE Purified Concentration: 1 mg/mL Reactivity: Human Laser: Not Applicable Peak Emission: Not Applicable Peak Excitation: Not Applicable Filter: Not Applicable Brightness (1=dim,5=brightest): Not Applicable Isotype: Mouse IgG1, kappa Formulation: Phosphate-buffered aqueous solution, ph7.2. Storage: Product should be kept at 2-8°C. Applications: FC, FA, IHC

Description

The 10.1 monoclonal antibody specifically reacts with human CD64, a 75kDA type I transmembrane glycoprotein that is the high affinity receptor for IgG. CD64 is expressed on macrophages and dendritic cells and is involved in antibody-dependent cellular toxicity, cytokine release phagocytosis, and endocytosis. The 10.1 antibody is reported to recognize the EC3 epitope of CD64.

Preparation & Storage The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinitychromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein. Application Notes The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. It is recommended that the reagent be titrated for optimal performance for each application. References

1.Schlossman, S. F. (1995).;Leucocyte typing V: White cell differentiation antigens: Proceedings of the Fifth International Workshop and Conference, Held in Boston, USA 3-7 November, 1993. Oxford University Press.

2. Dougherty, G. J., Selvendran, Y., Murdoch, S., Palmer, D. G., Hogg, N. (1987). The human mononuclear phagocyte high‐affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1.;European journal of immunology,17(10), 1453-1459.

3. Indik, Z. K., Hunter, S., Huang, M. M., Pan, X. Q., Chien, P., Kelly, C., ... Schreiber, A. D. (1994). The high affinity Fc gamma receptor (CD64) induces phagocytosis in the absence of its cytoplasmic domain: the gamma subunit of Fc gamma RIIIA imparts phagocytic function to Fc gamma RI.;Experimental hematology,;22(7), 599-606.