Saitozyma Wallum Fungal Planet Description Sheets 459

458 Persoonia – Volume 42, 2019

Saitozyma wallum Fungal Planet description sheets 459

Fungal Planet 945 – 19 July 2019 Saitozyma wallum Gogorza Gondra, J. Kruse, McTaggart, Boekhout & R.G. Shivas, sp. nov.

Etymology. Derived from the word ‘wallum’, which in the Kabi Kabi lan- Notes — Saitozyma wallum is the fifth species described in guage is the name for Banksia aemula, the plant species from which this this genus of basidiomycetous yeasts and filamentous fungi (Liu fungus was isolated in the Sunshine Coast, Australia. et al. 2015a). Saitozyma was proposed for yeasts in the flavus Classification — Trimorphomycetaceae, Tremellales, Tre clade sensu Liu et al. (2015b), which is equivalent to the pod mellomycetes. zolicus clade sensu Boekhout et al. (2011). Saitozyma contains species formerly assigned to Cryptococcus and Bullera, namely On MYPGA (Malt 0.3 %, Yeast 0.3 %, Peptone 0.5 %, Glucose C. flavus, C. paraflavus, C. podzolicus and B. ninhbinhensis. 1 %, Agar 1.5 %), after 5 d at 25 °C, colony is raised, smooth, Saitozyma wallum was isolated using a spore fall technique glossy, cream to white, 1–1.5 mm with an entire margin; cells (Pennycook & Newhook 1978) from the abaxial surface of a are subglobose to ellipsoidal, 2–5 × 1.5–3.5 μm, occurring leaf of Banksia aemula, collected in wallum heathland on Bribie singly or in small clusters and proliferating by polar budding Island. The wallum heathland is floristically diverse and endemi- on a narrow base. Sexual spores, pseudohyphae or hyphae cally rich, restricted to coastal parts of southern Queensland were not observed. Fermentation and assimilation of carbon and northern New South Wales (Keith et al. 2014). compounds – see MycoBank MB827331. Saitozyma wallum had high sequence identity to S. podzolica Typus. Australia, Queensland, Bribie Island, S27°00'11.3" E153°07'14.1", (GenBank NR_073213, 451/483 base pairs, 93 % in the ITS on leaves of Banksia aemula (Proteaceae), 21 Feb. 2018, R.A. Gogorza region; GenBank NG_058283.1, 847/894 base pairs, 95 % in Gondra, N.V. Wolter, M.D.E. Shivas & R.G. Shivas (holotype preserved the LSU region) and S. ninhbinhensis (GenBank AB261011, as metabolically inactive culture BRIP 66859; culture ex-type BRIP 66859, ITS and LSU sequences GenBank MH793357and MH793355, MycoBank 541/583 base pairs, 93 % in the LSU region) in a BLAST search MB827331). against sequences from ex-types. Saitozyma wallum was sister to S. podzolica (CBS 6819) and an as yet unpublished Saito zyma species (GenBank AB720988) isolated from the bark of a cinnamon tree in India. There was intraspecific diversity within S. wallum as evidenced by two SNPs in the ITS region of three specimens. Phylogram obtained from a maximum likelihood search in IQ-TREE v. 1.7 beta, with a GTR gamma FreeRate heterogeneity model of evolution and dif- ferent rates for ITS and LSU ribosomal DNA loci (command -spp -m GTR+R). aRLT values (≥ 0.9) and bootstrap support values (≥ 75 %) from 10 000 replicates are shown above nodes. Asterisks (*) indicate ex-type sequences.

Colour illustrations. Banksia aemula in wallum heathland on Bribie Island, Australia. Colonies on MYPG agar; budding cells. Scale bars = 1 cm, 1 mm, 10 μm.

R. Adrian Gogorza Gondra, P.O. Box 80125, 3508 TC Utrecht, University Utrecht, The Netherlands; e-mail: [email protected] Alistair R. McTaggart, Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St Lucia 4069, Australia; e-mail: [email protected] Teun Boekhout, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands and Institute of Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam, Amsterdam, The Netherlands; e-mail: [email protected] Julia Kruse & Roger G. Shivas, Centre for Crop Health, University of Southern Queensland, Toowoomba 4350, Australia; e-mail: [email protected] & [email protected]