Catalog # Aliquot Size

U207-911-05 3 x 5 nmol U207-911-20 3 x 20 nmol U207-911-50 3 x 50 nmol

UBA7 (UBE1L) siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # U207-911

Lot # Z2109-13

Specificity Formulation UBA7 (UBE1L) siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human UBA7 (UBE1L) expression. shipped at room temperature.

Product Description Reconstitution Protocol UBA7 (UBE1L) siRNA is a pool of three individual synthetic Briefly centrifuge the tubes (maximum RCF 4,000g) to siRNA duplexes designed to knock-down human UBA7 collect lyophilized siRNA at the bottom of the tube. (UBE1L) mRNA expression. Each siRNA is 19-25 bases in Resuspend the siRNA in 50 µl of DEPC-treated water length. The accession number is NM_003335. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot D8; UBE2; UBA1B; UBA7; UBE1L 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or UBA1 (UBE1) U201-30G below -20oC. After resuspension, siRNA stock solutions ≥2 UBA2 (SAE2) Protein U202-30G µM can undergo up to 50 freeze-thaw cycles without UBA3 (UBE1C) Protein U203-30G significant degradation. For long-term storage, it is UBA7 (UBE1L) Protein U207-30G recommended that the siRNA is stored at -70oC. For most Protein U06-54H favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background UBA7 or or ubiquitin-like modifier activating enzyme 7 is a UBA7 (UBE1L) siRNA Set I member of the E1 ubiquitin-activating enzyme family siRNA duplexes targeted against three exon regions which is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) Catalog # U207-911 degradation and apoptosis in acute promyelocytic Lot # Z2109-13 leukemia, where it is involved in the conjugation of the Packaging Specifications 2.5 nmol/tube for 3 x 5nmol ubiquitin-like interferon-stimulated gene 15 protein. UBA7 Format Lyophilized powder is expressed in a variety of tissues, including normal lung, Stability 1yr at -70oC from date of shipment but highly expressed in a large series of lung cancer cell Storage & Shipping The lyophilized powder is stable for at lines (1). UBA7 may represent a second, autosomal least 4 weeks at room temperature. It is member of the ubiquitin-activating gene family, which recommended that the lyophilized and resuspended siRNAs are stored at play a role in the ubiquitin conjugation pathway, which is or below -20oC. After resuspension, of central importance in all eukaryotes (2). siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For References long-term storage, it is recommended 1. Carritt, B.et.al: A gene from human that the siRNA is stored at -70oC. For most favorable performance, avoid region 3p21 with reduced expression in small cell lung repeated handling and multiple cancer. Cancer Res. 52: 1536-1541, 1992. freeze/thaw cycles. 2. Kok, K.et.al: A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme. Proc. Nat. Acad. Sci. 90: 6071-6075, 1993.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.