DOCSLIB.ORG

Production of Fish Cake from Siamese Mud Carp

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Production of Fish Cake from Siamese Mud Carp International Journal of Applied Engineering Research ISSN 0973-4562 Volume 13, Number 2 (2018) pp. 1332-1335 © Research India Publications. http://www.ripublication.com Production of Fish Cake from Siamese Mud Carp 1Minh N. P.*, and 2Nga N. H. 1Faculty of Food Technology - Biotech, Dong A University, Da Nang City, Vietnam. 2Department of Health, Soc Trang province, Vietnam. *Corresponding author Abstract The Siamese mud carp (Henicorhynchus siamensis) is a species of freshwater cyprinid fish, a variety of Asian From the tenth to eleventh months of the lunar calendar, the carp native to the Mekongand Chao Phraya flooding season comes to Mekong Delta. But that marks the Rivers in Southeast Asia, especially start of the migratory Siamese mud carp (Henicorhynchus in Cambodia, Laos, Thailand and Vietnam. Henicorhynchus siamensis) season, a vast multitude of fish species in the siamensis is a synchronous, i.e. single spawned species that Mekong River. In Vietnam, catching or farming aquatic clearly shows a single peak of gonadosomatic index in June to resources forms a vital part of rural people's livelihoods and September and the highest in August (Suvarnaraksha et al. contributes a major source of protein. With a huge volumn of 2010). During the wet season, this species migrates into Siamese mud carp ( ) caught a year, Henicorhynchus siamensis floodplains for spawning (Fukushima et al. 2014). Eggs and Vietnamese farmers ussually process them into fermented fish larvae grow in the floodplains and the larvae migrate back to sauce. There is not much research mentioned to processing rivers when the floodwaters begin to recede at the starting of this valuable fish. In order to improving the added value of dry season (Rainboth 1996; Fukushima et al. 2014). this fish, we investigated a production of fish cake from Henicorhynchus siamens is also adapts to lentic Siamese mud carp. By investigation different parameters environmental conditions, such as lakes and reservoirs, and probably affecting to gelation, we noticed that we would get contributes a significant portion in fish catches the best physicochemical properties and sensory (Suvarnaraksha et al. 2010). Not only does this fish provide characteristics of fish cake by the addition of carrageenan protein, but also vitamins and minerals to the people (Roos et 1.0%: gelatin 1.0%. al. 2007). Keywords: Siamese mud carp, fish cake, carrageenan, Siamese mud carp is a major source of income for many physicochemical properties gelatin, gelation, families in the delta in the flooding season (Piyathap Avakul, 2015). A family can catch 100 kg of the fish or above a day on average, which is retailed to local residents or wholesaled to INTRODUCTION traders. The volume of Siamese mud carp caught a year Hydrocolloids are a heterogeneous group of long chain depends on rainfall in the rainy season. Viraxay Bandavong et polymers (polysaccharides and proteins) characterised by their al. (2016) performed isolation and screening of protease property of forming viscous dispersions and/or gels when producing halotolerant bacteria from fermented freshwater dispersed in water. Presence of a large number of hydroxyl (- fishes. In this research, they used Siamese mud carp as a OH) groups markedly increases their affinity for binding material for examining. We didn’t see any research mentioned water molecules rendering them hydrophilic compounds. to fish cake production from Siamese mud carp. Therefore, we Further, they produce a dispersion, which is intermediate focused on examining different aspects affecting to siamese between a true solution and a suspension, and exhibits the mud carp cake production such as carrageenan, gelatin properties of a colloid. Hydrocolloids have a wide array of concentration supplemented into fish paste, mixture of functional properties in foods. These include thickening, carrageenan and gelatin. gelling, emulsifying, stabilisation, and controlling the crystal growth of ice and sugar though the basic properties for which hydrocolloids find extensive use are thickening and gelling. MATERIAL & METHOD Hydrocolloids disperse in water to give a thickening or Material viscosity producing effect. This water-thickening property is common to all hydrocolloids and is the prime reason for their We catched Siamese mud carp from An Giang province, overall use (Dipjyoti Saha, 2010). Carrageenan is a Vietnam.. After harvesting, they must be kept in ice chest (< hydrocolloid that finds maximum application because of its 4oC) and conveyed to laboratory within 2 hours for ability to form gels at much lower concentrations compared to experiments. Besides Siamese muad carp, we also used other any other gelling agent (Puvanenthiran et al. 2003; de materials such as NaCl, monosodium glutamate (MSG), Vries 2004; Verbeken et al 2004). Gelatin exhibits a wide pepper, sugar, garlic, carrageenan, gelatin. Lab utensils and range of functional properties. Gelatin gels melt at relatively equipments included grinder, weight balance, thermometer, low temperature (melt-in mouth). Gelatin melts at much lower autoclave, ice chest, texture analyzer. We evaluated moisture temperature because the junction zones are only bound by content, texture, color in fish cake. weak hydrogen bonds (Dipjyoti Saha, 2010). 1332 International Journal of Applied Engineering Research ISSN 0973-4562 Volume 13, Number 2 (2018) pp. 1332-1335 © Research India Publications. http://www.ripublication.com Research method Table 1. Effect of carrageenan concentration to texture firmness of fish cake Investigate the effect of carrageenan to physicochemical properties and sensory characteristics of fish cake Carrageenan Texture firmness of fish cake Siamese mud carp was kept under 4oC in 2 hours before being concentration (%) (g/mm2) grinded thoroughly. Salt 1%, MSG 0.2%, sugar 0.5%, pepper Control 8.72d 2% and garlic 2.5% were used as food ingredients. It was grinded into paste in 2 minutes at 0-4oC. Then we added 1.0 9.85c different carrageenan concentration (1.0, 1.5, 2.0, 2.5 and b 3.0%) into fish paste. Fish cake was then formed and 1.5 11.79 o sterilized at 121 C in 10 minutes. Texture, color, and moisture 2.0 12.01ab were tested to identify the optimal carrageenan concentration. 2.5 12.13a a Investigate the effect of gelatin to physicochemical 3.0 12.19 properties and sensory characteristics of fish cake Siamese mud carp was kept under 4oC in 2 hours before being Table 2. Effect of carrageenan concentration to color of fish grinded thoroughly. Salt 1%, MSG 0.2%, sugar 0.5%, pepper cake 2% and garlic 2.5% were used as food ingredients. It was grinded into paste in 2 minutes at 0-4oC. Then we added Carrageenan concentration Color of fish cake (a different gelatin concentration (1.0, 1.5, 2.0, 2.5 and 3.0%) (%) value) into fish paste. Fish cake was then formed and sterilized at Control 57.45c 121oC in 10 minutes. Texture, color, and moisture were tested 1.0 58.73bc to identify the optimal carrageenan concentration. 1.5 59.91b 2.0 62.13ab Investigate the effect of mixture (carrageenan: gelatin) to a physicochemical properties and sensory characteristics of 2.5 62.58 fish cake 3.0 62.67a Siamese mud carp was kept under 4oC in 2 hours before being grinded thoroughly. Salt 1%, MSG 0.2%, sugar 0.5%, pepper Table 3. Effect of carrageenan concentration to moisture 2% and garlic 2.5% were used as food ingredients. It was content of fish cake grinded into paste in 2 minutes at 0-4oC. Then we added different carragenan: gelatin ratio (2.0%:0%, 0:2.0%, Carrageenan concentration Moisture content of fish cake 1.0%:1.0%, 1.5%:0.5, 0.5%:1.5%, respectively) into fish (%) (%) paste. Fish cake was then formed and sterilized at 121oC in 10 Control 65.01c minutes. Texture, color, and moisture were tested to identify the optimal carrageenan concentration. 1.0 66.74b b 1.5 66.98 2.0 69.23a Statistical analysis 2.5 69.56a Data were statistically summarized by Statgraphics. 3.0 69.64a RESULT & DISCUSSION From table 1, 2 and 3 we will get the best physicochemical Effect of carrageenan to physicochemical properties and properties and sensory characteristics of fish cake by the sensory characteristics of fish cake addition of carrageenan 2.0%. Similarly, Nicomrat et al. (2016) demonstrated the effect of carrageenan on quality of Hydrocolloids are widely used in many food formulations to frozen Moo yor. The result showed that carrageenan favorably improve quality attributes and shelf-life. The two main uses affected cooking loss and expressible moisture content better are as thickening and gelling agents. Hydrocolloids with their than tapioca starch. unique characteristics are of great interest in processed meat due to their abilities to bind water and form gels (Candogan and Kolsarici, 2003). Effect of gelatin to physicochemical properties and sensory characteristics of fish cake Gel formation is the phenomenon involving the association or cross-linking of the polymer chains to form a three 1333 International Journal of Applied Engineering Research ISSN 0973-4562 Volume 13, Number 2 (2018) pp. 1332-1335 © Research India Publications. http://www.ripublication.com dimensional network that traps or immobilises the water Effect of mixture (carrageenan: gelatin) to within it to form a rigid structure that is resistant to flow. The physicochemical properties and sensory characteristics of formation of gel involves the association of randomly fish cake dispersed polymer segments in dispersion in such a way so as Table 7. Effect of carrageenan: gelatin ratio to texture to form a three-dimensional network that contains solvent in firmness of fish cake the interstices. Ratio of carrageenan:gelatin Texture firmness of fish cake (g/mm2) Table 4. Effect of gelatin concentration to texture firmness of c fish cake Carrageenan 2.0% 12.01 Gelatin 2.0% 11.97c Gelatin concentration (%) Texture firmness of fish a cake (g/mm2) Carrageenan 1.0%: Gelatin 1.0% 14.24 b Control 8.72d Carrageenan 1.5%: Gelatin 0.5% 13.17 b 1.0 9.93c Carrageenan 0.5%: Gelatin 1.5% 13.08 1.5 11.55b 2.0 11.97ab Table 8.
Load more

Recommended publications
  • Rede Nordeste De Biotecnologia Programa De Pós-Graduação Em Biotecnologia
    Rede Nordeste de Biotecnologia Programa de Pós-Graduação em Biotecnologia SULIMARY OLIVEIRA GOMES CARACTERIZAÇÃO GENÔMICA E GENÉTICA DO CARANGUEJO Cardisoma guanhumi (Crustacea, Decapoda, Brachyura) TERESINA 2016 SULIMARY OLIVEIRA GOMES CARACTERIZAÇÃO GENÔMICA E GENÉTICA DO CARANGUEJO Cardisoma guanhumi (Crustacea, Decapoda, Brachyura) Tese apresentada ao Programa de Pós-Graduação da Rede Nordeste de Biotecnologia – Embrapa Meio- Norte – Universidade Federal do Piauí, como requisito para a obtenção do grau de Doutor em Biotecnologia. Área de Concentração: Biotecnologia em Agropecuária Orientador: Dr. Fábio Mendonça Diniz Teresina 2016 1 FICHA CATALOGRÁFICA Serviço de Processamento Técnico da Universidade Federal do Piauí Biblioteca Comunitária Jornalista Carlos Castello Branco Serviço de Processamento Técnico G633c Gomes, Sulimary Oliveira. Caracterização genômica e genética do caranguejo Cardisoma guanhumi (Crustacea, Decapoda, Brachyura) / Sulimary Oliveira Gomes – 2016. 74 f ; il. Tese (Doutorado em Biotecnologia - RENORBIO) – Universidade Federal do Piauí, 2016. “Orientador Prof. Dr. Fábio Mendonça Diniz.” 1. Conservação. 2. Gecarcinidae. 3. Microssatélites. 4. Sequenciamento. I. Titulo. CDD 595.386 Título: CARACTERIZAÇÃO GENÔMICA E GENÉTICA DO CARANGUEJO Cardisoma guanhumi (Crustacea, Decapoda, Brachyura) Autor (a): Sulimary Oliveira Gomes Aprovado em: ___/___/_____ Banca examinadora: __________________________________________ Prof. Dr. Adalberto Socorro da Silva Universidade Federal do Piauí (Membro externo) __________________________________________
    [Show full text]
  • Analysis of Genome Survey Sequences and SSR Marker Development for Siamese Mud Carp, Henicorhynchus Siamensis, Using 454 Pyrosequencing
    Int. J. Mol. Sci. 2012, 13, 10807-10827; doi:10.3390/ijms130910807 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Article Analysis of Genome Survey Sequences and SSR Marker Development for Siamese Mud Carp, Henicorhynchus siamensis, Using 454 Pyrosequencing Feni Iranawati *,†, Hyungtaek Jung *,†, Vincent Chand, David A. Hurwood and Peter B. Mather Earth, Environmental and Biological Sciences, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Queensland 4000, Australia; E-Mails: [email protected] (V.C.); [email protected] (D.A.H.); [email protected] (P.B.M.) † These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: [email protected] (F.I.); [email protected] (H.J.); Tel.: +61-7-3138-4152 (F.I. & H.J.); Fax: +61-7-3138-2330 (F.I. & H.J.). Received: 5 July 2012; in revised form: 30 July 2012 / Accepted: 24 August 2012 / Published: 29 August 2012 Abstract: Siamese mud carp (Henichorynchus siamensis) is a freshwater teleost of high economic importance in the Mekong River Basin. However, genetic data relevant for delineating wild stocks for management purposes currently are limited for this species. Here, we used 454 pyrosequencing to generate a partial genome survey sequence (GSS) dataset to develop simple sequence repeat (SSR) markers from H. siamensis genomic DNA. Data generated included a total of 65,954 sequence reads with average length of 264 nucleotides, of which 2.79% contain SSR motifs. Based on GSS-BLASTx results, 10.5% of contigs and 8.1% singletons possessed significant similarity (E value < 10–5) with the majority matching well to reported fish sequences.
    [Show full text]
  • Henicorhynchus Lobatus Ecological Risk Screening Summary
    Henicorhynchus lobatus (a carp, no common name) Ecological Risk Screening Summary U.S. Fish and Wildlife Service, June 2012 Revised, September 2018 Web Version, 2/15/2019 Image: Smithsonian Institution. Licensed under CC BY-NC-SA 3.0. Available: http://eol.org/data_objects/18161264. (September 2018). 1 Native Range and Status in the United States Native Range From Baird and Allen (2011): “This species is widespread in the Mekong, and also found in the Mae Khlong and Chao Phraya basins.” “Native: Cambodia; Lao People's Democratic Republic; Thailand; Viet Nam” Status in the United States This species has not been reported as introduced or established in the United States. There is no indication that this species is in trade in the United States. Means of Introductions in the United States This species has not been reported as introduced or established in the United States. 1 Remarks The synonym Gymnostomus lobatus was also used when researching in preparation of this report. From Baird and Allen (2011): “Scientific Name: Gymnostomus lobatus (Smith, 1945)” “Considered by some authors to be in the genus Henicorhynchus, or Cirrhinus.” 2 Biology and Ecology Taxonomic Hierarchy and Taxonomic Standing From ITIS (2018): “Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Actinopterygii Class Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Henicorhynchus Species Henicorhynchus lobatus Smith, 1945” From Fricke et al. (2018): “Current status: Valid as Henicorhynchus lobatus Smith 1945. Cyprinidae: Labeoninae.” Size, Weight, and Age Range From Froese and Pauly (2018): “Max length : 15.0 cm SL male/unsexed; [Baird et al.
    [Show full text]
  • Impacts of Plastic Pollution on Freshwater Aquatic, Terrestrial and Avian Migratory Species in the Asia and Pacific Region | 1
    IMPACTS OF PLASTIC POLLUTION ON FRESHWATERN AQUATIC, TERRESTRIAL AND AVIAN MIGRATORY SPECIES IN W THE ASIA AND PACIFIC REGION E Impacts of Plastic Pollution on Freshwater Aquatic, Terrestrial and Avian Migratory Species in the Asia and Pacific Region | 1 Prepared for the Secretariat of the Convention on Migratory Species (CMS) by the National Oceanography Centre (NOC), UK, July 2021. AUTHORS Alice A Horton Isobelle Blissett ACKNOWLEDGEMENTS CMS Secretariat and CounterMEASURE II Migratory Species Focal Area Team: Clara Nobbe, Head of CMS Terrestrial Species Reynaldo Molina, Migratory Species Focal Area Coordinator Dr. Zeb Hogan, CMS COP-Appointed Councillor for Freshwater Fish Dr. Tilman Schneider, CMS Associate Programme Officer Andrea Dekrout, CMS EU Programme Manager CounterMEASURE II Scientific Advisory Group and Focal Areas: Prof. Dr. Atsuhiko Isobe, Dr. Shin'ichiro Kako, Dr. Purvaja Ramachandran, and Dr. Manmohan Sarin, Scientific Advisory Group Kakuko Yoshida, Chief Technical Advisor Makoto Tsukiji, Mekong Focal Area Coordinator Reuben Gergan, Ganges Focal Area Consultant Simon Beasley (NOC) for obtaining images. Dunia Sforzin (CMS) for the layout. Thanks go to all image owners for permission to use their images in this report. COVER IMAGE Indian Elephants: © Tharmapalan Tilaxan ISBN: 978-3-937429-32-8 © 2021 CMS. This publication may be reproduced in whole or in part and in any form for educational and other non-profit purposes without special permission from the copyright holder, provided acknowledgement of the source is made. The CMS Secretariat would appreciate receiving a copy of any publication that uses this publication as a source. No use of this pu- blication may be made for resale or for any other commercial purposes whatsoever without prior permission from the CMS Secretariat.
    [Show full text]
  • Geometric Morphometrics and Phylogeny of the Catfish Genus Mystus Scopoli (Siluriformes:Bagridae) and North American Cyprinids (Cypriniformes)
    Geometric Morphometrics and Phylogeny of the Catfish genus Mystus Scopoli (Siluriformes:Bagridae) and North American Cyprinids (Cypriniformes) by Shobnom Ferdous A dissertation submitted to the Graduate Faculty of Auburn University in partial fulfillment of the requirements for the Degree of Doctor of Philosophy Auburn, Alabama December 14, 2013 Copyright 2013 by Shobnom Ferdous Approved by Jonathan W. Armbruster, Department of Biological Sciences, Auburn University & Committee Chair Craig Guyer, Department of Biological Sciences, Auburn University Michael C. Wooten, Department of Biological Sciences, Auburn University Lawrence M. Page, Curator of Fishes, Florida Museum of Natural History Abstract Understanding the evolution of organismal form is a primary concern of comparative biology, and inferring the phylogenetic history of shape change is, therefore, a central concern. Shape is one of the most important and easily measured elements of phenotype, and shape is the result of the interaction of many, if not most, genes. The evolution of morphological traits may be tightly linked to the phylogeny of the group. Thus, it is important to test the phylogenetic dependence of traits to study the relationship between traits and phylogeny. My dissertation research has focused on the study of body shape evolution using geometric morphometrics and the ability of geometric morphometrics to infer or inform phylogeny. For this I have studied shape change in Mystus (Siluriformes: Bagridae) and North American cyprinids. Mystus Scopoli 1771 is a diverse catfish group within Bagridae with small- to medium-sized fishes. Out of the 44 nominal species worldwide, only 30 are considered to be part of Mystus. Mystus is distributed in Turkey, Syria, Iraq, Iran, Afghanistan, Pakistan, India, Nepal, Sri Lanka, Bangladesh, Myanmar, Thailand, Malay Peninsula, Vietnam, Sumatra, Java and Borneo.
    [Show full text]
  • Life History of the Riverine Cyprinid Henicorhynchus Siamensis (Sauvage, 1881) in a Small Reservoir by A
    Journal of Applied Ichthyology J. Appl. Ichthyol. (2010), 1–6 Received: December 20, 2009 Ó 2010 Blackwell Verlag, Berlin Accepted: August 17, 2010 ISSN 0175–8659 doi: 10.1111/j.1439-0426.2010.01619.x Life history of the riverine cyprinid Henicorhynchus siamensis (Sauvage, 1881) in a small reservoir By A. Suvarnaraksha1,2,3, S. Lek2, S. Lek-Ang2 and T. Jutagate1 1Faculty of Agriculture, Ubon Ratchathnai University, Warin Chamrab, Ubon Ratchathani, Thailand; 2University of Toulouse III, Laboratoire Dynamique de la Biodiversite´, CNRS – UPS, Toulouse Cedex, France; 3Faculty of Fisheries Technology and Aquatic Resources, Maejo University, Sansai, Chiangmai, Thailand Summary candidates for a fish stock enhancement program to increase The riverine species, Henicorhynchus siamensis (Sauvage 1881), fish production in inland water bodies in the region (Jutagate, is an important source of protein and an economical fish for 2009). the rural population of inland Indochina. Investigated in the Knowledge of the life cycles of many important Southeast present study were the reproductive feeding aspects and Asia freshwater fish species is still very fragmentary, especially growth of H. siamensis living in a lake system. The gonado- when they inhabit an uncommon environment (Volbo-Jørgen- somatic index peaked in August, which was delayed compared sen and Poulsen, 2000). Given the importance of H. siamensis to river fish, and individuals took 1.5 years to attain the length to fisheries in many parts of major Southeast Asian rivers, this of 50% maturity (about 200 mm). Stomach contents were study aimed to investigate the key facets of the H. siamensis dominated by phytoplankton and showed considerable sea- life history (Froese et al., 2000), i.e.
    [Show full text]
  • Analysis of Genome Survey Sequences and SSR Marker Development for Siamese Mud Carp, Henicorhynchus Siamensis, Using 454 Pyrosequencing
    Int. J. Mol. Sci. 2012, 13, 10807-10827; doi:10.3390/ijms130910807 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Article Analysis of Genome Survey Sequences and SSR Marker Development for Siamese Mud Carp, Henicorhynchus siamensis, Using 454 Pyrosequencing Feni Iranawati *,†, Hyungtaek Jung *,†, Vincent Chand, David A. Hurwood and Peter B. Mather Earth, Environmental and Biological Sciences, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Queensland 4000, Australia; E-Mails: [email protected] (V.C.); [email protected] (D.A.H.); [email protected] (P.B.M.) † These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: [email protected] (F.I.); [email protected] (H.J.); Tel.: +61-7-3138-4152 (F.I. & H.J.); Fax: +61-7-3138-2330 (F.I. & H.J.). Received: 5 July 2012; in revised form: 30 July 2012 / Accepted: 24 August 2012 / Published: 29 August 2012 Abstract: Siamese mud carp (Henichorynchus siamensis) is a freshwater teleost of high economic importance in the Mekong River Basin. However, genetic data relevant for delineating wild stocks for management purposes currently are limited for this species. Here, we used 454 pyrosequencing to generate a partial genome survey sequence (GSS) dataset to develop simple sequence repeat (SSR) markers from H. siamensis genomic DNA. Data generated included a total of 65,954 sequence reads with average length of 264 nucleotides, of which 2.79% contain SSR motifs. Based on GSS-BLASTx results, 10.5% of contigs and 8.1% singletons possessed significant similarity (E value < 10–5) with the majority matching well to reported fish sequences.
    [Show full text]
  • Genes Involved in Sex Determination Process
    Vol. 14(5), pp. 400-411, 4 February, 2015 DOI: 10.5897/AJB2014.14116 Article Number: 03298F150279 ISSN 1684-5315 African Journal of Biotechnology Copyright © 2015 Author(s) retain the copyright of this article http://www.academicjournals.org/AJB Full Length Research Paper Development of microsatellite markers for use in breeding catfish, Rhamdia sp. Marília Danyelle Nunes Rodrigues1*, Carla Giovane Ávila Moreira2, Harold Julian Perez Gutierrez1, Diones Bender Almeida3, Danilo Streit Jr.4 and Heden Luiz Marques Moreira3 1Laboratório de Engenharia Genética Animal – LEGA/Instituto de Biologia/Programa de Pós Graduação em Zootecnia. Universidade Federal de Pelotas; Pelotas, RS. Brasil. 2Programa Pós-graduação em Genética e Biologia Molecular – Universidade Federal do Rio Grande do Sul. LEGA/ Universidade Federal de Pelotas; Pelotas, RS. Brasil. 3Laboratório de Engenharia Genética Animal – LEGA/Instituto de Biologia. Universidade Federal de Pelotas; Pelotas, RS. Brasil. 4Universidade Federal do Rio Grande do Sul. Departamento de Zootecnia, Universidade Federal do Rio Grande do Sul; Porto Alegre, RS, Brasil. Received 20 August, 2014; Accepted 26 January, 2015 Microsatellites markers for catfish, Rhamdia sp. were developed using Next Generation. A shotgun paired-end library was prepared according to standard protocol of Illumina Nextera DNA Library Kit with dual indexing, paired-end reads of 100 base pairs and grouped with other species. From a single race, five million readings obtained were analyzed with the program PAL_FINDER_v0.02.03. perl script was used to extract readings containing microsatellites with di-, tri-, tetra-, penta- and hexanucleotides from five million readings obtained in sequencing. Readings were grouped and used in Primer3 version 2.0.0 to design primers when GC content was greater than 30%, melting temperatures was within 58 to 65°C, with 2°C maximum difference between primers, the last 2 nucleotides in 3’extremity are G or C, and maximum poli-N of 4 nucleotides.
    [Show full text]
  • Mud Carp (Henicorhynchus Siamensis and H. Lobatus) Elucidated by Otolith Microchemistry
    Potential Effects of Hydroelectric Dam Development in the Mekong River Basin on the Migration of Siamese Mud Carp (Henicorhynchus siamensis and H. lobatus) Elucidated by Otolith Microchemistry Michio Fukushima1*, Tuantong Jutagate2, Chaiwut Grudpan2, Pisit Phomikong2, Seiichi Nohara1 1 National Institute for Environmental Studies, Tsukuba, Japan, 2 Department of Fisheries, Faculty of Agriculture, Ubon Ratchathani University, Ubon Ratchathani, Thailand Abstract The migration of Siamese mud carp (Henicorhynchus siamensis and H. lobatus), two of the most economically important fish species in the Mekong River, was studied using an otolith microchemistry technique. Fish and river water samples were collected in seven regions throughout the whole basin in Thailand, Laos and Cambodia over a 4 year study period. There was coherence between the elements in the ambient water and on the surface of the otoliths, with strontium (Sr) and barium (Ba) showing the strongest correlation. The partition coefficients were 0.409–0.496 for Sr and 0.055 for Ba. Otolith Sr- Ba profiles indicated extensive synchronized migrations with similar natal origins among individuals within the same region. H. siamensis movement has been severely suppressed in a tributary system where a series of irrigation dams has blocked their migration. H. lobatus collected both below and above the Khone Falls in the mainstream Mekong exhibited statistically different otolith surface elemental signatures but similar core elemental signatures. This result suggests a population originating from a single natal origin but bypassing the waterfalls through a passable side channel where a major hydroelectric dam is planned. The potential effects of damming in the Mekong River are discussed. Citation: Fukushima M, Jutagate T, Grudpan C, Phomikong P, Nohara S (2014) Potential Effects of Hydroelectric Dam Development in the Mekong River Basin on the Migration of Siamese Mud Carp (Henicorhynchus siamensis and H.
    [Show full text]
  • Download E-Book (PDF)
    African Journal of Biotechnology Volume 14 Number 5, 4 February, 2015 ISSN 1684-5315 ABOUT AJB The African Journal of Biotechnology (AJB) (ISSN 1684-5315) is published weekly (one volume per year) by Academic Journals. African Journal of Biotechnology (AJB), a new broad-based journal, is an open access journal that was founded on two key tenets: To publish the most exciting research in all areas of applied biochemistry, industrial microbiology, molecular biology, genomics and proteomics, food and agricultural technologies, and metabolic engineering. Secondly, to provide the most rapid turn-around time possible for reviewing and publishing, and to disseminate the articles freely for teaching and reference purposes. All articles published in AJB are peer- reviewed. Submission of Manuscript Please read the Instructions for Authors before submitting your manuscript. The manuscript files should be given the last name of the first author Click here to Submit manuscripts online If you have any difficulty using the online submission system, kindly submit via this email [email protected]. With questions or concerns, please contact the Editorial Office at [email protected]. Editor-In-Chief Associate Editors George Nkem Ude, Ph.D Prof. Dr. AE Aboulata Plant Breeder & Molecular Biologist Plant Path. Res. Inst., ARC, POBox 12619, Giza, Egypt Department of Natural Sciences 30 D, El-Karama St., Alf Maskan, P.O. Box 1567, Crawford Building, Rm 003A Ain Shams, Cairo, Bowie State University Egypt 14000 Jericho Park Road Bowie, MD 20715, USA Dr. S.K Das Department of Applied Chemistry and Biotechnology, University of Fukui, Japan Editor Prof. Okoh, A. I. N.
    [Show full text]
  • Análise Do Crescimento E Identificação De Loci Potencialmente Amplificáveis Em Peixe-Rei
    0 UNIVERSIDADE FEDERAL DE PELOTAS Programa de Pós-Graduação em Zootecnia Tese Análise do crescimento e identificação de loci potencialmente amplificáveis em peixe-rei Rafael Aldrighi Tavares Pelotas, 2014 1 RAFAEL ALDRIGHI TAVARES Análise do crescimento e identificação de loci potencialmente amplificáveis em peixe-rei Tese apresentada ao Programa de Pós- Graduação em Zootecnia da Universidade Federal de Pelotas, como requisito parcial à obtenção do título de Doutor em Ciências (Área de conhecimento: Melhoramento Genético Animal). Orientador: Prof. Dr. Nelson José Laurino Dionello Co-Orientador: Prof. Dr. Heden Luiz Marques Moreira Pelotas, 2014 Universidade Federal de Pelotas / Sistema de Bibliotecas Catalogação na Publicação T231a Tavares, Rafael Aldrighi TavAnálise do crescimento e identificação de loci potencialmente amplificáveis em peixe-rei / Rafael Aldrighi Tavares ; Nelson José Laurino Dionello, orientador ; Heden Luiz Marques Moreira, coorientador. — Pelotas, 2014. Tav55 f. TavTese (Doutorado) — Programa de Pós-Graduação em Zootecnia, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 2014. Tav1. Melhoramento genético. 2. Marcadores moleculares. 3. Peixes nativos. 4. Híbrido. I. Dionello, Nelson José Laurino, orient. II. Moreira, Heden Luiz Marques, coorient. III. Título. CDD : 639.3 Elaborada por Gabriela Machado Lopes CRB: 10/1842 2 Banca Examinadora - Dr. Nelson José Laurino Dionello - Dr. Sérgio Renato Noguez Piedras - Dr. Cleber Bastos Rocha - Dr. Juvêncio Luis Osório Fernandes Pouey - Dr. Diones Bender Almeida 3 “A cada dia a natureza produz o suficiente para nossa carência. Se cada um tomasse o que lhe fosse necessário, não havia pobreza no mundo e ninguém morreria de fome”. (Ghandi) 4 Dedico esse trabalho a minha esposa, minha filha, meus familiares, amigos e professores, que juntos proporcionaram esse momento de realização.
    [Show full text]
  • Fisheries Report
    USAID Mekong ARCC Climate Change Impact and Adaptation Study Fisheries Report February 2014 This publication was produced for submission to the United States Agency for International Development. It was prepared for USAID Mekong ARCC by ICEM and DAI. The contents of this document are the sole responsibility of ICEM and DAI and do not necessarily reflect the views of the US Government. USAID Mekong ARCC Climate Change Impact and Adaptation Study Fisheries Report Program Title: USAID Mekong Adaptation and Resilience to Climate Change (USAID Mekong ARCC) Sponsoring USAID Office: USAID/Asia Regional Environment Office Contract Number: AID-486-C-11-00004 Contractor: DAI Date of Publication: February 2014 This publication has been made possible by the support of the American People through the United States Agency for International Development (USAID). The contents of this document are the sole responsibility of International Centre for Environmental Management (ICEM) and Development Alternatives Inc. (DAI) and do not necessarily reflect the views of USAID or the United States Government. USAID MEKONG ARCC CLIMATE CHANGE IMPACT AND ADAPTATION STUDY Citation: ICEM (2013) USAID Mekong ARCC Climate Change Impact and Adaptation on Fisheries. Prepared for the United States Agency for International Development by ICEM - International Centre for Environmental Management Study team: Jeremy Carew-Reid (Team Leader), Tarek Ketelsen (Modeling Theme Leader), Jorma Koponen, Mai Ky Vinh, Simon Tilleard, Toan To Quang, Olivier Joffre (Agriculture Theme Leader), Dang Kieu Nhan, Bun Chantrea, Rick Gregory (Fisheries Theme Leader), Meng Monyrak, Narong Veeravaitaya, Truong Hoanh Minh, Peter-John Meynell (Natural Systems Theme Leader), Sansanee Choowaew, Nguyen Huu Thien, Thomas Weaver (Livestock Theme Leader), John Sawdon (Socio-economics Theme Leader),Try Thuon, Sengmanichanh Somchanmavong, and Paul Wyrwoll The USAID Mekong ARCC project is a five- year program (2011-2016) funded by the USAID Regional Development Mission for Asia (RDMA) in Bangkok.
    [Show full text]