21.10.2014ACVR1B antibody (pAb)

Rabbit Anti-Human/Mouse Activin A Receptor Type IB (ACTR-IB, ACVRLK4, ALK4, ALK -4)

Instruction Manual

Catalog Number PK-AB718-4793 Synonyms ACVR1B Antibody: Activin A receptor type IB, -like 4, Activin receptor type 1B precursor, ACTR-IB, ACVRLK4, ALK4, ALK-4 Description Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling . Activins signal through a heteromeric complex of receptor which include at least two type I and two type II receptors. ACVR1B, also known as activin receptor-like kinase 4 (ALK4), is a type I receptor for activin and plays major roles in cell differentiation, growth arrest and apoptosis. Like another type I activin receptor ACVR1C, ACVR1B can mediate signaling by ligands such as Nodal, Xnr1, GDF- 1/3, activin B and activin AB. In Xenopus embryos, expression of a dominant-negative form of ACVR1B blocked all mesoderm-inducing ligands, while expression of a dominant negative ACVR1C only blocked Nodal and Xnr1 signaling, suggesting that the ACVR1B and ACVR1C possess distinct functions. At least three isoforms of ACVR1B are known to exist. This antibody is predicted to have no cross-reactivity to ACVR1 or ACVR1C. Quantity 100 µg Source / Host Rabbit Immunogen ACVR1B antibody was raised against a 14 amino acid peptide near the carboxy terminus of the human ACVR1B. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse Specificity At least three isoforms of ACVR1B are known to exist. This antibody is predicted to have no cross- reactivity to ACVR1 or ACVR1C. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. ACVR1B antibody can be used for detection of ACVR1B by Western blot at 1 µg/mL. Images Available upon request. References Tsuchida K, Sawchenko PE, Nishikawa S, et al. Molecular cloning of a novel type I receptor serine/ kinase for the TGF beta superfamily from rat brain. Mol. Cell. Neurosci. 1996; 7:467–78. Reissmann E, Jornvall H, Blokzijl A, et al. The orphan receptor ALK7 and the activin receptor ALK4 mediate signaling by proteins during vertebrate development. Dev. 2001; 15:2010– 22. Tsuchida K, Nakatani M, Yamakawa N, et al. Activin isoforms signal through type I receptor serine/threonine kinase ALK7. Mol. Cell Endocrinol. 2004; 220:59-65.

Images Available upon request. Related Products Cat.No. PK-AB718-4793P - ACVR1B Peptide Cat. No. PK-AB718-1305 - Human Kidney Tissue Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014