MASTDISCS® ID Interpretation of results Nitrocefin Discs Positive – Development of a red colour in the area of the disc where the culture was applied. Note the D59 colour change does not normally develop over the whole of the disc. Intended use Negative – No colour change. For the rapid detection of -lactamase enzymes in isolated colonies of Neisseria gonorrhoeae, , A positive result should be interpreted as resistance to Staphylococcus spp., Haemophilus influenzae and or activity. Susceptibility should anaerobic . be confirmed by standard growth-dependent susceptibility testing methods. Negative results imply but do not FOR IN VITRO DIAGNOSTIC USE ONLY guarantee susceptibility.

Contents Quality control 50 discs in a light-resistant plastic vial (D59). Check for signs of deterioration. Quality control must be performed with at least one organism to demonstrate a Formulation* positive reaction and at least one organism to demonstrate 6mm diameter filter paper discs impregnated with a negative reaction. Do not use the product if the Nitrocefin. reactions with the control organisms are incorrect. The list below illustrates a range of performance control strains Storage and shelf life which the end user can easily obtain. Store in the freezer below minus 10C in the containers provided until the expiry date shown on the pack label. Test Organisms Result Allow to equilibrate to room temperature before opening Bacteroides fragilis Positive then return to freezer storage immediately after use. NCTC 9343 Staphylococcus aureus Positive Precautions NCTC 12973 For in vitro diagnostic use only. Observe approved Clostridoides difficile Negative biohazard precautions and aseptic techniques. To be NCTC 11204 used only by adequately trained and qualified laboratory personnel. Sterilise all biohazard waste before disposal. Limitations Refer to Product Safety Data sheet. For most bacterial strains a positive results will develop within 5 minutes. However, positive reactions for some Materials required but not provided staphylococci and anaerobic species may take up to 60 Standard microbiological supplies and equipment such as minutes to develop. loops, MAST® culture media, swabs, applicator sticks, incinerators and incubators, etc., as well as serological Detection of staphylococcal -lactamase is enhanced by and biochemical reagents and additives such as blood. testing growth from around the outer edge of the zone of inhibition of an Oxacillin 1 µg disc (MASTDISCS® AST Procedure OX1 or OX1C). 1. Place the required number of Nitrocefin discs into a clean empty Petri dish or onto a microscope slide. References 2. Discs may be moistened with one drop of deionised Bibliography available on request. water. Do not over-moisten. 3. Using a sterile loop or applicator stick remove several well-isolated and similar colonies and smear onto the surface of a disc. Alternatively: moisten the disc with one drop of deionised water, then holding the disc in forceps, wipe across a colony on an agar plate. 4. Observe the inoculated disc for the development of a red colour.

IFU117 GB 06/20 V6 MAST is a Registered Trademark ATCC is a trademark of the American Type Culture Collection, Manassas, Virginia, USA *Formulation may change to meet performance criteria