RANGAP1 Antibody Cat. No.: 57-264

RANGAP1 Antibody

Western blot analysis of RANGAP1 using rabbit polyclonal RANGAP1 Antibody immunohistochemistry analysis in RANGAP1 Antibody using 293 cell lysates (2 ug/lane) either formalin fixed and paraffin embedded human colon tissue nontransfected (Lane 1) or transiently transfected (Lane 2) followed by peroxidase conjugation of the secondary with the RANGAP1 . antibody and DAB staining.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

This RANGAP1 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 20-49 amino acids from the N-terminal region of human RANGAP1.

TESTED APPLICATIONS: IHC-P, WB

For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50

September 30, 2021 1 https://www.prosci-inc.com/rangap1-antibody-57-264.html PREDICTED MOLECULAR 64 kDa WEIGHT:

Properties

This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: RANGAP1

ALTERNATE NAMES: GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD

ACCESSION NO.: P46060

PROTEIN GI NO.: 1172922

GENE ID: 5905

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 BACKGROUND: gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.

REFERENCES: 1) Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008)

2) Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007)

3) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

September 30, 2021 2 https://www.prosci-inc.com/rangap1-antibody-57-264.html 4) Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)

ANTIBODIES FOR RESEARCH USE ONLY.

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September 30, 2021 3 https://www.prosci-inc.com/rangap1-antibody-57-264.html