RANGAP1 Antibody Cat. No.: 57-264
RANGAP1 Antibody
Western blot analysis of RANGAP1 using rabbit polyclonal RANGAP1 Antibody immunohistochemistry analysis in RANGAP1 Antibody using 293 cell lysates (2 ug/lane) either formalin fixed and paraffin embedded human colon tissue nontransfected (Lane 1) or transiently transfected (Lane 2) followed by peroxidase conjugation of the secondary with the RANGAP1 gene. antibody and DAB staining.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
This RANGAP1 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 20-49 amino acids from the N-terminal region of human RANGAP1.
TESTED APPLICATIONS: IHC-P, WB
For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50
September 30, 2021 1 https://www.prosci-inc.com/rangap1-antibody-57-264.html PREDICTED MOLECULAR 64 kDa WEIGHT:
Properties
This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: RANGAP1
ALTERNATE NAMES: Ran GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD
ACCESSION NO.: P46060
PROTEIN GI NO.: 1172922
GENE ID: 5905
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 BACKGROUND: gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
REFERENCES: 1) Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008)
2) Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007)
3) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
September 30, 2021 2 https://www.prosci-inc.com/rangap1-antibody-57-264.html 4) Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)
ANTIBODIES FOR RESEARCH USE ONLY.
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September 30, 2021 3 https://www.prosci-inc.com/rangap1-antibody-57-264.html