Journal of Pure & Applied Sciences www.Suj.sebhau.edu.ly ISSN 2521-9200 Received 12/05/2019 Revised 21/08/2019 Published online 25/11/2019

Lentinula edodes

[email protected]

Lentinula

PDA Potato Dextrose Agar edodes 7 3 24±0.1 02

02 2.0 8 02 7 . 00 Lentinula edodes Training on commercial mushroom Propagation: Shitake mushroom Lentinula edodes as a model *S. Isslugi , A. Eshtawe , R. Rashid Department of Botany, Faculty of Science, University of Tripoli, Libya *Corresponding author: [email protected] Abstract For the purpose of training on commercial propagation of mushroom mycelium, a biopsy of mushroom Lentinula edodes was taken from the bottom of the cap and injected into PDA Potato Dextrose Agar in Petri dishes which were then incubated at 24±0.1C. After 3 days of injection, growth of the mushroom mycelium began to spread. After 7 days, a halo of mycelium was formed around the tissue biopsy. After 20 days, growth of the mushroom mycelium was completed on the whole of the dish. Due to the fact, commercial form of mycelium which is used in mushroom farms involves sterilized cereal seeds, Mycelium of every Petri dish was divided into 8 discs which were then mixed with sterilized wheat seeds in flasks and incubated at 24±0.1C. Shiitake mushroom mycelium began to emerge and spread after 7 days, spread clearly within 14 days and colonized the wheat seeds completely after 21 days. The seeds were then ready to be used for inoculation of compost in mushroom farms. Keywords: Cultivation of Shiitake mushroom, Lentinula edodes, Small and medium incubators.

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0 0222 022 32 Lentinula edodes Agaricus spp Pleurotus spp. -2 02 02 30 : 1-2 , Autoclave 3-1 1 3 1 , , .Agar, Dextrose 22 Culture media: 2 -2 :Potato Dextrose Agar (PDA) 50g Potato [7]. 5g Dextrose 5g Agar lentinan 2 3 1 . Lentinula cm9 02 022 02 022 0 0 [4] 1-3-3 Dextrose 2 Agar 2 . 1-3-4 82 . 1-3-5 000 Autoclave . 02 02 1-3-6 0 cm9 PDA

0 . 1-3-7 . : Lentinula edodes 1 4 Kingdom: Mycota Phylum: Class: Order: Family: Genus: Lentinula Species: Lentinula edodes : 1-5

PDA 1 2

JOPAS Vol.18 No. 4 2019 201 Training on commercial mushroom Propagation: Shitake mushroom Lentinula edodes…. Isslugi et al.

Mother culture 2 4

Lacto phenol

: 5-2 Mother culture

PDA 2 2

3 2

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2 3

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5 2

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PDA 3 8

2 6

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PDA 8 3

00 3 (3-12). 9 3 PDA

[6] [1]

9 3 3 PDA Sylvan : 3-2 222 Lentinula edodes 02 3

[5]

11 3

11 3

: 3-3

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