ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

VOLUME 4 * NUMBER 2 * AUGUST 1973 EDITORIAL BOARD GLADYS L. HOBBY, Editor-in-Chief (1975) U. S. Veterans Administration and the Infectious Disease Research Institute, East Orange, N.J. WALTER D. CELMER, Editor (1975) EDWARD W. HOOK, Editor (1976) Pfizer Inc., Groton, Conn. University of Virginia, Charlottesville JOEL G. FLAKS, Editor (1976) LEON H. SCHMIDT, Editor (1974) University of Pennsylvania, Philadelphia Southern Research Institute, Birmingham, Ala.

Fred Allison, Jr. (1973) M. Gorman (1974) T. J. Perun (1974) Theodore Anderson (1973) H. Grisebach (1974) Burton M. Pogeli (1975) Robert Austrian (1974) Arthur P. Grollman (1975) K. E. Price (1973) John Bennett (1973) Jack Gwaltney (1974) C. Reilly (1975) Konrad E. Bloch (1975) Fred Hahn (1973) R. W. Rickards (1974) E. Borowski (1973) William Hewitt (1974) R. W. Riddell (1974) D. Buyske (1974) Lillian V. Holdeman (1976) Richard Roberts (1973) Bernard E. Cabana (1975) Richard Hornick (1974) L. Sabath (1973) Yves Chabbert (1974) Milton Huppert (1974) Arthur K. Saz (1973) Patricia Charache (1974) George Gee Jackson (1974) F. C. Sciavolino (1973) Ernest Chick (1973) Keith Jensen (1974) Piero Sensi (1974) Leighton E. Cluff (1974) E. H. Kass (1973) Robert W. Sidwell (1975) Kenneth W. Cochran (1975) Donald Kaye (1973) David Smith (1974) Frank Collins (1973) Wm. Kirby (1973) Sigmund S. Socransky (1974) John Corcoran (1974) Vernon Knight (1974) Earle Spaulding (1974) Julian Davies (1973) Calvin Kunin (1975) Gene Stolierman (1974) Bernard Davis (1973) Mark Lepper (1974) R. Sutherland (1973) Rober DesPrez (1973) Robert G. Loudon (1974) Morton N. Swartz (1973) Liebor Ebringer (1974) Thomas H. Maren (1974) Ralph Tompsett (1973) H. L. Ennis (1974) Lester Mitscher (1974) John P. Utz (1973) David S. Feingold (1975) S. Mitsuhashi (1974) D. Vazquez (1974) Maxwell Finland (1973) R. B. Morin (1973) G. H. Wagman (1974) Robert J. Fitzgerald (1974) Bernard Moss (1975) B. Weisblum (1973) Anthony J. Glazko (1975) L. Nickell (1974) Temple Williams (1973) Harry Gooder (1973)

ROBERT A. DAY, Managing Editor, 1913 I St., N. W., Washington, D.C. 20006

EX OFFICIO L. LEON CAMPBELL, President (1973-1974) PHILIPP GERHARDT, Vice-President (1973-1974) DONALD E. SHAY, Secretary T. J. CARSKI, Treasurer

Antimicrobial Agents and Chemotherapy, a publication of N.W., Washington, D.C. 20006 (area 202 833-9416). Corre- the American Society for Microbiology, 1913 I St., N.W., spondence from ASM members relating to membership Washington, D.C. 20006, is devoted to the dissemination of dues, member subscriptions, changes of address, incorrect knowledge relating to all aspects of antimicrobial agents and journals, etc., should be directed to the Executive Secretary, chemotherapy, including cancer chemotherapy. Antimicro- American Society for Microbiology, 1913 I St., N.W., Wash- bial Agents and Chemotherapy is published monthly, and ington, D.C. 20006. Published monthly by the ASM at 428 the twelve numbers are divided into two volumes per year. E. Preston St., Baltimore, Md. 21202. The subscription price is $40 (foreign, $41) per year. Single copies are $4.00 (foreign, $4.25). Members of the American Society for Microbiology mnay receive Antimicrobial Agents and Chemotherapy as part of their dues. Correspondence Second class postage paid at Washington, D.C. 20006, and relating to subscriptions, reprints, defective copies, avail- at additional mailing offices. ability of back issues, lost or late proofs, disposition of sub- Made in the United States of America. mitted manuscripts, and general editorial matters should Copyright i 1973, American Society for Microbiology be directed to the ASM Publications Office, 1913 I St., All Rights Reserved. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY INSTRUCTIONS TO AUTHORS Submit manuscripts in duplicate (original and one Abstract. An Abstract appears at the beginning of carbon) to ASM Publications Office, 1913 I St., N.W., each paper. The Abstract should not exceed 250 words. Washington, D.C. 20006. Literature Cited. In the text, references are cited by General policy. Any manuscript submitted must be number. The Literature Cited section should be typed a report of unpublished original research, which is not in alphabetical order, by first author, and numbered. being considered for publication elsewhere. Each Names ofjournals are abbreviated according to Chem- manuscript should present the results of an independ- ical Abstracts Service Source Index (American Chemi- ent, cohesive study; "series" papers are discouraged. cal Society, 1970). Citations of abstracts, theses, "un- A manuscript accepted and published by this journal published data," "personal communication," and must not be published again in any form without the "in press" will not be accepted in the Literature Cited. consent of ASM. Tables. Each table should be typed on a separate A charge of $25 per printed page is assessed for page. The data should be arranged so that columns of publication. Most institutions and granting agencies like material read down, not across. The headings in the United States permit the payment of publication should be sufficiently clear so that the meaning of the charges as a part of their general research support. It data will be understandable without reference to the is recognized, however, that such funds are not always text. Explanatory footnotes are permitted, but detailed available to laboratories outside the United States. descriptions of the experiments are not. The materials In such cases, the cost of publication will be borne by and methods used to gain the data should properly the American Society for Microbiology. remain in the section of that name. The "editorial style" of this journal essentially fol- Figures. A complete set of figures, preferably glossy lows the CBE Style Manual (3rd ed., AIBS, 1972). photographs, should accompany each of the two Genetics symbols should essentially follow the recom- copies of the manuscript. Each figure should be num- mendations of Demerec et al. (Genetics 54:61, 1966) bered and should include the name of the author, as updated and used by Taylor (Bacteriol. Rev. 34:155, either in the margin or on the back (marked lightly 1970) and Sanderson (Bacteriol. Rev. 34:176, 1970). with a soft pencil). Graphs (submit as photographs) The standard italicized, lower-case, three-letter symbol should be finished drawings not needing further art- should be used for genotype designation, and care work or type-setting. Absolutely no part of a graph should be taken to avoid using this symbol for the should be typewritten (except the legend, which should designation of phenotype. The latter can be abbrevi- be typed on a separate page). All lettering should be ated in one form or another, but should be stated in done with a lettering set. Most graphs will be reduced words at first use in the text. Biochemical abbreviations to one-column width, and all elements in the drawing and nomenclature should essentially follow "Bio- should be prepared to withstand this reduction. The chemical Nomenclature" in Handbook ofBiochemistry legend of the figure should provide enough informa- (2nd ed., 1970. H. A. Sober, ed. The Chemical Rubber tion so that the figure is understandable without Co., Cleveland, p. A4-A24). Normally, abbreviations reference to the text. Experimental details from Mate- (except those of standard units of measurement and rials and Methods should not be repeated in figure symbols of the elements) should be defined and intro- legends. duced parenthetically at first use in the text. Enzyme Nomenclature of microorganisms. The name of a activity should be expressed in terms of international species is a binary combination consisting of the name units (Enzyme Nomenclature, Elsevier Publishing Co., of the genus followed by a specific epithet. In general, 1965), and the EC number should be given parenthet- the nomenclature presented in Bergey's Manual oj ically at first use in the text. In expressing lengths, Determinative Bacteriology (7th ed., 1957) is used. If weights, and volumes, the prefixes nano (n) and pico an author challenges this nomenclature, his own judg- (p) should be used instead of millimicro (m,u) and ment will be followed, but the name in Bergey's micromicro (;,A). Express lengths in nanometers (nm; Manual should follow in parentheses the first time the 109 m) or in micrometers (Mm; 10" m) instead of name is used in the text and in the Abstract. millimicrons (mnu; 10-im), microns (i: 10 m), or Notes. The accepted form for Notes is somewhat Angstroms (A; 10-1im). Express parts per million different from the foregoing. Contributors should con- (ppm) as micrograms per milliliter (isg/ml), micro- sult a recent issue for style. Notes should not exceed grams per gram (i,g/g), or microliters per liter (pliters/ 500 words. The Abstract should not exceed 25 words. liter), as appropriate. In general, measurements should Copyright. Once a paper has been published in this be expressed in terms of standard international metric journal, which is a copyrighted publication, the legal units. The journal reserves the privilege of editing ownership of all parts of the paper, including the illus- manuscripts to make them conform with the adopted trations, has passed from the author to the ASM. If style. the same author, or any author, wishes to republish Form of manuscript. All parts of the manuscript material previously published in this journal, he must should be typed double-space or, preferably, triple- first receive written permission from ASM. space. Most manuscripts can and should be divided Reprints. Reprints (in multiples of 100) may be pur- into the following sections: Abstract, Introduction, chased by contributors. A table showing the cost of Materials and Methods, Results, Discussion, Ac- reprints, and an order form, will be sent with the knowledgments, and Literature Cited. proof.