Systematics of Spinybacked Treefrogs (Hylidae
Zoologica Scripta Systematics of spiny-backed treefrogs (Hylidae: Osteocephalus): an Amazonian puzzle JULIAN FAIVOVICH,JOSE M. PADIAL,SANTIAGO CASTROVIEJO-FISHER,MARIANA M. LYRA,BIANCA V. M. BERNECK,CELIO F. B. HADDAD,PATRICIA P. IGLESIAS,PHILIPPE J. R. KOK,ROSS D. MACCULLOCH, MIGUEL T. RODRIGUES,VANESSA K. VERDADE,CLAUDIA P. TORRES GASTELLO,JUAN CARLOS CHAPARRO,PAULA H. VALDUJO,STEFFEN REICHLE,JIRI MORAVEC,VACLAV GVOZDIK,GIUSSEPE GAGLIARDI-URRUTIA,RAFFAEL ERNST,IGNACIO DELARIVA,DONALD BRUCE MEANS, ~ ALBERTINA P. LIMA,J.CELSA SENARIS &WARD C. WHEELER Submitted: 17 September 2012 Jungfer, K.-H., Faivovich, J., Padial, J. M., Castroviejo-Fisher, S., Lyra, M.L., Berneck, Accepted: 5 April 2013 B.V.M., Iglesias, P.P., Kok, P. J. R., MacCulloch, R. D., Rodrigues, M. T., Verdade, doi:10.1111/zsc.12015 V. K., Torres Gastello, C. P., Chaparro, J. C., Valdujo, P. H., Reichle, S., Moravec, J., Gvozdık, V., Gagliardi-Urrutia, G., Ernst, R., De la Riva, I., Means, D. B., Lima, A. P., Senaris,~ J. C., Wheeler, W. C., Haddad, C. F. B. (2013). Systematics of spiny-backed treefrogs (Hylidae: Osteocephalus): an Amazonian puzzle. —Zoologica Scripta, 00, 000–000. Spiny-backed tree frogs of the genus Osteocephalus are conspicuous components of the tropi- cal wet forests of the Amazon and the Guiana Shield. Here, we revise the phylogenetic rela- tionships of Osteocephalus and its sister group Tepuihyla, using up to 6134 bp of DNA sequences of nine mitochondrial and one nuclear gene for 338 specimens from eight coun- tries and 218 localities, representing 89% of the 28 currently recognized nominal species. Our phylogenetic analyses reveal (i) the paraphyly of Osteocephalus with respect to Tepuihyla, (ii) the placement of ‘Hyla’ warreni as sister to Tepuihyla, (iii) the non-monophyly of several currently recognized species within Osteocephalus and (iv) the presence of low (<1%) and overlapping genetic distances among phenotypically well-characterized nominal species (e.g.
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