Polyclonal Antibody to ITIH1 / IGHEP1 (Center) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP52255PU-N Polyclonal Antibody to ITIH1 / IGHEP1 (Center) - Aff - Purified

Alternate names: ITI heavy chain H1, Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Inter-alpha-trypsin inhibitor heavy chain H1, Serum-derived hyaluronan-associated protein Quantity: 0.4 ml Concentration: lot specific Background: The protein encoded by this gene is the heavy chain of a serine protease inhibitor that may serve to carry hyaluronan in plasma. This gene is part of a cluster of similar genes on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. Uniprot ID: P19827 NCBI: NP_001159906 GeneID: 3697 Host / Isotype: Rabbit / Ig Immunogen: KLH conjugated synthetic peptide between 613-643 amino acids from the Central region of Human ITIH1 / IGHEP1 Format: State: Liquid purified Ig fraction Purification: Protein A column, followed by peptide affinity purification Buffer System: PBS containing 0.09% (W/V) Sodium Azide as preservative Applications: ELISA: 1/1000. Western Blot: 1/100-1/500. Flow Cytometry: 1/10-1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognizes Human ITIH1 / IGHEP1 (Center). Add. Information: Molecular Weight: 101389 Da Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: So, H.C., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (1), 103-113 (2010) Scott, L.J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(18):7501-7506(2009) Hamm, A., et al. BMC Cancer 8, 25 (2008): Scarchilli, L., et al. J. Biol. Chem. 282(41):30161-30170(2007) Bunkenborg, J., et al. Proteomics 4(2):454-465(2004)

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130815 1 / 2 AP52255PU-N: Polyclonal Antibody to ITIH1 / IGHEP1 (Center) - Aff - Purified

Pictures: Western blot analysis of ITIH1 / IGHEP1 Antibody (Center) Cat.-No AP52255PU-N in HL-60 cell line lysates (35ug/lane). This demonstrates the ITIH1 antibody detected the ITIH1 protein (arrow).

Flow cytometric analysis of HL-60 cells using ITIH1 / IGHEP1 Antibody (Center) Cat.-No AP52255PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti- rabbit secondary antibodies were used for the analysis.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130815 2 / 2