Anti-TANK2 / Tankyrase 2 Antibody (ARG64774)

ARG64774 Package: 100 μg anti-TANK2 / Tankyrase 2 antibody Store at: -20°C

Summary

Product Description Goat Polyclonal antibody recognizes TANK2 / Tankyrase 2

Tested Reactivity Hu

Predict Reactivity Ms, Rat, Dog

Tested Application WB

Specificity Fusion protein containing 73 amino acids from region amino acid 800-900 (according to NP_079511.1). No crossreactivity expected with TANK1.

Host Goat

Clonality Polyclonal

Isotype IgG

Target Name TANK2 / Tankyrase 2

Antigen Species Human

Immunogen Recombinant fusion protein containing 73 a.a. from region a.a. 800-900 (according to NP_079511.1)

Conjugation Un-conjugated

Alternate Names ARTD6; PARP5B; TRF1-interacting ankyrin-related ADP-ribose polymerase 2; Tankyrase-related protein; PARP5C; TNKS-2; Tankyrase-like protein; EC 2.4.2.30; Poly [ADP-ribose] polymerase 5B; Tankyrase II; pART6; TANK2; Tankyrase-2; PARP-5c; ADP-ribosyltransferase diphtheria toxin-like 6; PARP-5b; TNKL

Application Instructions

Application Note WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw 127 kDa

Properties

Form Liquid

Purification Purified from goat serum by ammonium sulphate precipitation followed by affinity chromatography using Protein G.

Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA

Preservative 0.02% Sodium azide

Stabilizer 0.5% BSA

Concentration 0.5 mg/ml

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links GeneID: 80351 Human

Swiss-port # Q9H2K2 Human

Gene Symbol TNKS2

Gene Full Name tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2

Function Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP- ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP- ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Stimulates 26S proteasome activity. [UniProt]

Research Area Gene Regulation antibody; Signaling Transduction antibody

Images

ARG64774 anti-TANK2 antibody WB image

Western Blot: 1). Mock transfection; 2) TANK2 (lane 4) and TANK1 (lane 2) expressing plasmid transfected HEK293 cell lysate standed with ARG64774 anti-TANK2 antibody. Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4°C.