Lot Number: RD2184992 Reticulon-1A (MON160) Product Data Sheet Tested Species Reactivity Details Hamster (Hm) Catalog Number: MA1-06810 (Hu) Size: 100 µL Mouse (Ms) Class: Monoclonal Rat (Rt) Type: Antibody Clone: MON160 Tested Applications Dilution * Host / Isotype: Mouse / IgG1 Western Blot (WB) 1:50-1:500 Partially purified bacterially Immunocytochemistry (ICC) Assay Dependent Immunogen: expressed reticulon-1A (NSP-A) hybrid Immunohistochemistry (Frozen) 1:50-1:100 (IHC (F)) Immunohistochemistry (Paraffin) 1:50-1:100 Form Information (IHC (P)) Form: Liquid Flow Cytometry (Flow) 1:50-1:100 Concentration: 1mg/ml * Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls. Purification: purified Storage Buffer: PBS Preservative: 0.09% sodium azide Storage Conditions: 4°C or -20°C if preferred

Product Specific Information General Information MA1-06810 detects reticulon-1A (NSP-A) in human, mouse, hamster, and Recently, a novel gene family has been identified and characterized, rat samples. designated the Reticulons because the encoded by these genes are anchored to the membranes of the . Reticulon-1 was MA1-06810 has sucessfully been used in Western blotting, FACS, ICC and formerly designated NSP for Neuroendocrine-Specific-Protein, because it is immunohistochemistry procedures. By Western blot it detects a ~135 kDa specifically expressed in neural and neuroendocrine tissues. The NSP-gene band representing reticulon-1A. has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, The MA1-06810 immunogen is a partially purified bacterially expressed i.e. reticulon-1A (NSP-A), reticulon-1B (NSP-B), and reticulon-1C reticulon-1A (NSP-A) hybrid protein. (NSP-C). These proteins were found to be anchored to membranes of the For Research Use Only. Not for use in diagnostic procedures. Not for endoplasmic reticulum through their common carboxy-terminal regions. resale without express authorization. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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