Supplementary materials: Exploring the Panx1 interactome

Exploring the Pannexin 1 interactome: In silico cross-analyses with postsynaptic and neuropsychiatric disorder susceptibility

Simona D Frederiksen1, Leigh E Wicki-Stordeur1, Juan C Sanchez-Arias1, and Leigh Anne Swayne1

1Division of Medical Sciences, University of Victoria, Victoria, BC Canada

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Supplementary materials: Exploring the Panx1 interactome

Fig. S1: Comprehensive overview of the workflow within the current study from research questions, material and methods to results.

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Supplementary materials: Exploring the Panx1 interactome

Fig. S2: Mouse STRING -protein interaction (PPI) network of 99 Panx1-interacting proteins located at the postsynapse. Eight out of the 16 clusters within the PPI network were composed of at least 5 Panx1-interacting proteins.

Reference

Szklarczyk, D., Gable, A.L., Lyon, D., Junge, A., Wyder, S., Huerta- Cepas, J., Simonovic, M., Doncheva, N.T., Morris, J.H., Bork, P., et al. (2019). STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic acids research 47 (D1), D607-D613.

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Supplementary materials: Exploring the Panx1 interactome

Table S1: Fifty-four Panx1-interacting proteins within the overrepresented intercellular protein transport (GO:0006886) GO term.

UniProt ID Gene symbol Protein description P68033 Actc1 , alpha cardiac muscle 1 P61164 Actr1a Alpha-centractin Q8R5C5 Actr1b Beta-centractin Q99JY9 Actr3 Actin-related protein 3 Q641P0 Actr3b Actin-related protein 3B P61205 Arf3 ADP-ribosylation factor 3 P84084 Arf5 ADP-ribosylation factor 5 P35564 Canx Calnexin Q3UX62 Ccdc114 Coiled-coil domain-containing protein 114 Q9D7S9 Chmp5 Charged multivesicular body protein 5 Q9Z0H8 Clip2 CAP-Gly domain-containing linker protein 2 O55029 Copb2 subunit beta' Q9ERK4 Cse1l Exportin-2 Q8BZN6 Dock10 Dedicator of cytokinesis protein 10 P50396 Gdi1 GDP dissociation inhibitor alpha Q9D7M1 Gid8 Glucose-induced degradation protein 8 homolog P61979 Hnrnpk Heterogeneous nuclear ribonucleoprotein K Q8VI75 Ipo4 Importin-4 Q60960 Kpna1 Importin subunit alpha-5 O35345 Kpna6 Importin subunit alpha-7 P70168 Kpnb1 Importin subunit beta-1 Q91YK0 Lrrc49 Leucine-rich repeat-containing protein 49 P46735 Myo1b Unconventional -Ib P21271 Myo5b Unconventional myosin-Vb P08556 Nras GTPase NRas P57722 Pcbp3 Poly(rC)-binding protein 3 P62492 Rab11A Ras-related protein Rab11A P46638 Rab11B Ras-related protein Rab11B P35283 Rab12 Ras-related protein Rab12 Q91V41 Rab14 Ras-related protein Rab14 Q8K386 Rab15 Ras-related protein Rab15 P62821 Rab1A Ras-related protein Rab1A Q923S9 Rab30 Ras-related protein Rab30 O35963 Rab33B Ras-related protein Rab33B Q6PHN9 Rab35 Ras-related protein Rab35 Q9JKM7 Rab37 Ras-related protein Rab37 Q8BHD0 Rab39A Ras-related protein Rab39A Q8BHC1 Rab39B Ras-related protein Rab39B Q9CZT8 Rab3B Ras-related protein Rab3B P62823 Rab3C Ras-related protein Rab3C P35276 Rab3D Ras-related protein Rab3D Q8CG50 Rab43 Ras-related protein Rab43 P56371 Rab4A Ras-related protein Rab4A P35279 Rab6A Ras-related protein Rab6A

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Supplementary materials: Exploring the Panx1 interactome

P61294 Rab6B Ras-related protein Rab6B P55258 Rab8A Ras-related protein Rab8A Q9D4V7 Rabl3 Rab-like protein 3 P62835 Rap1A Ras-related protein Rap-1A Q9CQS8 Sec61b Protein transport protein Sec61 subunit beta Q99LG2 Tnpo2 Transportin-2 Q6PAM1 Txlna Alpha-taxilin Q80XC3 Usp6nl USP6 N-terminal-like protein Q9D2N9 Vps33a -associated protein 33A P59016 Vps33b Vacuolar protein sorting-associated protein 33B

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Supplementary materials: Exploring the Panx1 interactome

Table S2: Thirty-five Panx1-interacting proteins within the overrepresented vesicle-mediated transport (GO:0016192) GO term.

UniProt ID Gene symbol Protein description P68033 Actc1 Actin, alpha cardiac muscle 1 P61164 Actr1a Alpha-centractin Q8R5C5 Actr1b Beta-centractin Q99JY9 Actr3 Actin-related protein 3 Q641P0 Actr3b Actin-related protein 3B P61205 Arf3 ADP-ribosylation factor 3 P84084 Arf5 ADP-ribosylation factor 5 P35564 Canx Calnexin Q9Z0H8 Clip2 CAP-Gly domain-containing linker protein 2 O08585 Clta light chain A O55029 Copb2 Coatomer subunit beta' Q9QXK3 Copg2 Coatomer subunit gamma-2 Q8BZN6 Dock10 Dedicator of cytokinesis protein 10 P50396 Gdi1 Rab GDP dissociation inhibitor alpha Q8VD75 Hip1 Huntingtin-interacting protein 1 P17879 Hspa1b Heat shock 70 kDa protein 1B P16627 Hspa1l Heat shock 70 kDa protein 1-like P17156 Hspa2 Heat shock-related 70 kDa protein 2 Q9CQM2 Kdelr2 ER lumen protein-retaining receptor 2 P46735 Myo1b Unconventional myosin-Ib P21271 Myo5b Unconventional myosin-Vb P08556 Nras GTPase NRas P46460 Nsf Vesicle-fusing ATPase P35283 Rab12 Ras-related protein Rab12 P56371 Rab4A Ras-related protein Rab4A P35279 Rab6A Ras-related protein Rab6A P61294 Rab6B Ras-related protein Rab6B P55258 Rab8A Ras-related protein Rab8A P62835 Rap1A Ras-related protein Rap1A O08547 Sec22b Vesicle-trafficking protein SEC22b Q9D662 Sec23b Protein transport protein Sec23B Q6PAM1 Txlna Alpha-taxilin Q9D2N9 Vps33a Vacuolar protein sorting-associated protein 33A P59016 Vps33b Vacuolar protein sorting-associated protein 33B

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Supplementary materials: Exploring the Panx1 interactome

Table S3: Thirty-seven Panx1-interacting proteins within the overrepresented GTPase activity (GO:0003924) GO term.

UniProt ID Gene symbols Protein description P58252 Eef2 2 Q8C0D5 Efl1 Elongation factor-like GTPase 1 P21278 Gna11 Guanine nucleotide-binding protein subunit alpha-11 P27600 Gna12 Guanine nucleotide-binding protein subunit alpha-12 P27601 Gna13 Guanine nucleotide-binding protein subunit alpha-13 Q8CGK7 Gnal Guanine nucleotide-binding protein G(olf) subunit alpha P62880 Gnb2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 Q60766 Irgm1 Immunity-related GTPase family M protein 1 P08556 Nras GTPase NRas P62492 Rab11A Ras-related protein Rab11A P46638 Rab11B Ras-related protein Rab11B P35283 Rab12 Ras-related protein Rab12 Q91V41 Rab14 Ras-related protein Rab14 Q8K386 Rab15 Ras-related protein Rab15 P62821 Rab1A Ras-related protein Rab1A Q923S9 Rab30 Ras-related protein Rab30 O35963 Rab33B Ras-related protein Rab33B Q6PHN9 Rab35 Ras-related protein Rab35 Q9JKM7 Rab37 Ras-related protein Rab37 Q8BHD0 Rab39A Ras-related protein Rab39A Q8BHC1 Rab39B Ras-related protein Rab39B Q9CZT8 Rab3B Ras-related protein Rab3B P62823 Rab3C Ras-related protein Rab3C P35276 Rab3D Ras-related protein Rab3D Q8BMG7 Rab3gap2 Rab3 GTPase-activating protein non-catalytic subunit Q8CG50 Rab43 Ras-related protein Rab43 P56371 Rab4A Ras-related protein Rab4A P35279 Rab6A Ras-related protein Rab6A P61294 Rab6B Ras-related protein Rab6B P55258 Rab8A Ras-related protein Rab8A Q9D4V7 Rabl3 Rab-like protein 3 P62835 Rap1A Ras-related protein Rap1A Q9D662 Sec23b Protein transport protein Sec23B P42209 Sept1 Septin-1 P42208 Sept2 Septin-2 Q80UG5 Sept9 Septin-9 Q80XC3 Usp6nl USP6 N-terminal-like protein

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