For Research Use Only
PFKM Polyclonal antibody Catalog Number:55028-1-AP Featured Product 27 Publications www.ptglab.com
Catalog Number: GenBank Accession Number: Purification Method: Basic Information 55028-1-AP NM_000289 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 600 μg/ml by 5213 WB 1:500-1:3000 Nanodrop and 527 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:3000 method using BSA as the standard; phosphofructokinase, muscle for WB Source: IHC 1:20-1:200 Calculated MW: IF 1:10-1:100 Rabbit 85 kDa Isotype: Observed MW: IgG 75-85 kDa
Applications Tested Applications: Positive Controls: IF, IHC, IP, WB,ELISA WB : human skeletal muscle tissue, HeLa cells, HepG2 Cited Applications: cells, human brain tissue IF, WB IP : mouse skeletal muscle tissue, mouse liver tissue Species Specificity: IHC : human skeletal muscle tissue, human, mouse, rat Cited Species: IF : HeLa cells, human, mouse, pig, rat Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and Two domains Background Information subfamily. PFKM catalyze the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular mass of 85, 82 and 93 kDa. The antibody is specific to PFKM.
Notable Publications Author Pubmed ID Journal Application Wei Xu 32935679 Food Funct WB Pengjuan Zhang 31714070 Anal Chem WB Min Peng 34022203 Exp Cell Res IF
Storage: Storage Store at -20°C. Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage
For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data
human skeletal muscle tissue were subjected to WB result of PFKM antibody (55028-1-AP; 1:1000; IP Result of anti-PFKM (IP:55028-1-AP, 4ug; SDS PAGE followed by western blot with 55028-1- incubated at room temperature for 1.5 hours) with Detection:55028-1-AP 1:1500) with mouse skeletal AP (PFKM antibody) at dilution of 1:800 incubated sh-Control and sh-PFKM transfected HeLa cells. muscle tissue lysate 2200ug. at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin- Immunohistochemical analysis of paraffin- Immunofluorescent analysis of HeLa cells using embedded human skeletal muscle tissue slide embedded human skeletal muscle tissue slide 55028-1-AP (PFKM antibody) at dilution of 1:25 and using 55028-1-AP (PFKM Antibody) at dilution of using 55028-1-AP (PFKM Antibody) at dilution of Rhodamine-Goat anti-Rabbit IgG. 1:50 (under 10x lens). 1:50 (under 40x lens).