Kir2.2 (KCNJ12) (NM 021012) Human Tagged ORF Clone Product Data

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Kir2.2 (KCNJ12) (NM_021012) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: Kir2.2 (KCNJ12) (NM_021012) Human Tagged ORF Clone Tag: TurboGFP Symbol: KCNJ12 Synonyms: hIRK; hIRK1; hkir2.2x; IRK-2; IRK2; kcnj12x; KCNJN1; Kir2.2; Kir2.2v Vector: pCMV6-AC-GFP (PS100010) E. coli Selection: Ampicillin (100 ug/mL) Cell Selection: Neomycin ORF Nucleotide >RG207051 representing NM_021012 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGACCGCGGCCAGCCGGGCCAACCCCTACAGCATCGTGTCATCGGAGGAGGACGGGCTGCACCTGGTCA CCATGTCGGGCGCCAACGGCTTCGGCAACGGCAAGGTGCACACGCGGCGCAGGTGCCGCAACCGCTTCGT CAAGAAGAATGGCCAGTGCAACATTGAGTTCGCCAACATGGACGAGAAGTCACAGCGCTACCTGGCTGAC ATGTTCACCACCTGTGTGGACATCCGCTGGCGGTACATGCTGCTCATCTTCTCGCTGGCCTTCCTTGCCT CCTGGCTGCTGTTCGGCATCATCTTCTGGGTCATCGCGGTGGCACACGGTGACCTGGAGCCGGCTGAGGG CCGGGGCCGCACACCCTGTGTGATGCAGGTGCACGGCTTCATGGCGGCCTTCCTCTTCTCCATCGAGACG CAGACCACCATCGGCTACGGGCTGCGCTGTGTGACGGAGGAGTGCCCGGTGGCCGTCTTCATGGTGGTGG CCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGTGCCATCATGGCCAAGATGGCAAGGCC CAAGAAGCGGGCACAGACGCTGCTGTTCAGCCACAACGCCGTGGTGGCCCTGCGTGACGGCAAGCTCTGC CTCATGTGGCGTGTGGGTAACCTGCGCAAGAGCCACATTGTGGAGGCCCATGTGCGCGCGCAGCTCATCA AGCCGCGGGTCACCGAGGAGGGCGAGTACATCCCGCTGGACCAGATCGACATCGATGTGGGCTTCGACAA GGGCCTGGACCGCATCTTTCTGGTGTCGCCCATCACCATCTTGCATGAGATTGACGAGGCCAGCCCGCTC TTCGGCATCAGCCGGCAGGACCTGGAGACGGACGACTTTGAGATCGTGGTCATCCTGGAAGGCATGGTGG AGGCCACAGCCATGACCACCCAGGCCCGCAGCTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTT TGAGCCCGTGCTCTTCGAGGAGAAGAACCAGTACAAGATTGACTACTCGCACTTCCACAAGACCTATGAG GTGCCCTCTACGCCCCGCTGCAGTGCGAAGGATCTGGTAGAGAACAAGTTCCTGCTGCCCAGCGCCAACT CCTTCTGCTACGAGAACGAGCTGGCCTTCCTGAGCCGTGACGAGGAGGATGAGGCGGACGGAGACCAGGA CGGCCGAAGCCGGGACGGCCTCAGCCCCCAGGCCAGGCATGACTTTGACAGACTCCAGGCTGGCGGCGGG GTCCTGGAGCAGCGGCCCTACAGACGGGAGTCAGAGATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 3 Kir2.2 (KCNJ12) (NM_021012) Human Tagged ORF Clone – RG207051

Protein Sequence: >RG207051 representing NM_021012 Red=Cloning site Green=Tags(s)

MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLAD MFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVMQVHGFMAAFLFSIET QTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLC LMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPL FGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYE VPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGG VLEQRPYRRESEI

TRTRPLE - GFP Tag - V

Chromatograms: https://cdn.origene.com/chromatograms/ja2656_f04.zip Restriction Sites: SgfI-MluI Cloning Scheme:

Plasmid Map:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 3 Kir2.2 (KCNJ12) (NM_021012) Human Tagged ORF Clone – RG207051

ACCN: NM_021012 ORF Size: 1299 bp OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at [email protected] or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_021012.5 RefSeq Size: 5230 bp RefSeq ORF: 1302 bp Locus ID: 3768 UniProt ID: Q14500 Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane Gene Summary: This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith- Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]